Journal: Clinical Cancer Research

Article Title: Unique Cell Surface Expression of Receptor Tyrosine Kinase ROR1 in Human B-Cell Chronic Lymphocytic Leukemia

doi: 10.1158/1078-0432.ccr-07-1823

Figure Lengend Snippet: Fig. 2. ROR1protein expression on the surface of B-CLL cells. A, flow cytometry profiles of PBMC from a representative B-CLL patient. Four-color flow cytometry was carried out with a mouse anti-human CD19 mAb conjugated to PE, a mouse anti-human CD5 mAb conjugated toAPC, gahROR1pAb or gahROR2 pAb, swine anti-goat-FITC conjugate, and propidium iodide.The histograms on the right represent staining for ROR1 (green) and ROR2 (pink) in CD5+ CD19+ B-CLL cells (top) and CD5+ CD19- Tcells (bottom).The background signal with normal goat immunoglobulin or secondary antibody alone is shownin black. B, biotinylated cell surface proteins from B-CLL cells or normal B cells were separated by SDS-PAGE. Subsequent Western blotting with gahROR1pAb and donkey anti-goat-HRP conjugate detected a single band of f120 kDa in B-CLL cells (left lane). No band was detected in normal B cells (right lane). C, B-CLL cells were incubated with gahROR1pAb for1h at 4jC. Subsequently, the cells were washed and either left at 4jC (green) or incubated for 1h at 37jC in the absence (red) or presence (blue) of 3 Amol/L phenylarsine oxide, followed by flow cytometry analysis with swine anti-goat-FITC conjugate.The background signal with secondary antibody alone is shown in black.

Article Snippet: Affinity-purified polyclonal goat anti-human ROR1 (gahROR1 pAb), polyclonal goat anti-human ROR2 antibodies (gahROR2 pAb), and polyclonal goat anti-human CD5 antibodies (gahCD5 pAb) were used as primary antibodies (R&D Systems).

Techniques: Expressing, Flow Cytometry, Staining, SDS Page, Western Blot, Incubation

Journal: iScience

Article Title: Increased peritoneal B1-like cells during acute phase of human septic peritonitis

doi: 10.1016/j.isci.2024.110133

Figure Lengend Snippet:

Article Snippet: anti-CD5-VioBlue clone REA421 , Miltenyi Biotec , cat#130-123-287; RRID: AB_2811489.

Techniques: Recombinant, Blocking Assay, Software