Journal: Annals of the Rheumatic Diseases

Article Title: Premature senescence of T-cell subsets in axial spondyloarthritis

doi: 10.1136/annrheumdis-2014-206119

Figure Lengend Snippet: Telomere lengths are prematurely reduced in naïve CD4 + T cells of patients with aSpA and RA

Article Snippet: In a second step, CD45RA + and CD45RO + T cells were obtained via positive selection using CD45RA or CD45RO Microbeads (Miltenyi).

Techniques:

Journal: Annals of the Rheumatic Diseases

Article Title: Premature senescence of T-cell subsets in axial spondyloarthritis

doi: 10.1136/annrheumdis-2014-206119

Figure Lengend Snippet: Multivariate regression analysis for telomere lengths

Article Snippet: In a second step, CD45RA + and CD45RO + T cells were obtained via positive selection using CD45RA or CD45RO Microbeads (Miltenyi).

Techniques: Biomarker Discovery

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Direct stimulation of human T cells via TLR5 and TLR7/8: flagellin and R-848 up-regulate proliferation and IFN-gamma production by memory CD4+ T cells.

doi: 10.4049/jimmunol.175.3.1551

Figure Lengend Snippet: FIGURE 5. Memory T cells are more sensitive to Pam3CSK4, flagellin, and R-848 than naive T cells. Naive (CD45RA) () and memory (CD45RO) T cells (u) were purified from CD4 T cells by FACS sorting and either unstimulated or stimulated with IL-2 (100 U/ml for cytokine production or 20 U/ml for proliferation assay) or anti-CD2 mAbs (2 g/ml) in the absence or presence of 1 g/ml Pam3CSK4, 5 g/ml flagellin, or 1 g/ml R-848. IFN- was quantified in the 48-h cell-free supernatants (A), and proliferation was determined after 72 h (B). Results are expressed as mean SD, n 3, and are representative of the data obtained with the T cells of two of six healthy donors.

Article Snippet: CD4 CD45RO T cells were separated into CCR7 and CCR7 cells by FACS sorting using FITC-labeled anti-CD45RO mAb and PE-labeled antiCCR7 mAb (R&D Systems).

Techniques: Proliferation Assay

Journal: The Veterinary Quarterly

Article Title: Bovine Lymphocyte Intestinal Retention Defect (BLIRD): a novel recessive immunogenetic disorder in Holstein cattle

doi: 10.1080/01652176.2025.2566997

Figure Lengend Snippet: Blood count and tissue distribution of LPAM1 pos CD4 T cells in LRS and LRF/LRC animals. (A) White blood cell count (in 10 9 cells/L) in LRS and LRF/LRC animals. (B) Blood cell counts (in 10 9 cells/L) of lymphocytes, monocytes, neutrophils, eosinophils, and basophils in LRS (n = 36) and LRF/LRC (n = 40) animals. (C) Representative flow cytometry plots of CD45RO + cells among CD4 + T lymphocytes in peripheral blood (PBMCs) from LRS (n = 3) and LRF/LRC (n = 4) animals. (D) Representative flow cytometry plots of α4 + β7 + cells among CD45RO + CD4 + T cells in peripheral blood (PBMCs), mesenteric lymph node (mLN) and jejunal lamina propria (jLP) from LRS (n = 3) and LRF/LRC (n = 4) animals. (E) Quantification of CD45RO + cells among CD4 + T lymphocytes in peripheral blood (PBMCs) from LRS (n = 3) and LRF/LRC (n = 4) animals. (F) Quantification of α4 + β7 + cells among CD45RO + CD4 + T cells in peripheral blood (PBMCs), mesenteric lymph node (mLN) and jejunal lamina propria (jLP) from LRS (n = 3) and LRF/LRC (n = 4) animals.

Article Snippet: The antibodies used were: CD45 FITC (BioRad, Cat#MCA2220), CD4 Pacific Blue (BioRad, Cat#MCA1653), CD45RO Alexa Fluor 647 (BioRad, Cat#MCA2434), α4 integrin PE/Cyanine7 (BioLegend, Cat#304314) and integrin β7 PE (BioLegend, Cat#121006).

Techniques: Cell Counting, Flow Cytometry

Journal: Cell reports

Article Title: Mass Cytometry Reveals Global Immune Remodeling with Multi-lineage Hypersensitivity to Type I Interferon in Down Syndrome

doi: 10.1016/j.celrep.2019.10.038

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Mouse monoclonal anti-human CD45RO (clone UCHL1) , Fluidigm , Cat#3164007B; RRID:AB_2811092.

Techniques: Recombinant, Mass Cytometry, Software

Journal: Cardiovascular Diabetology

Article Title: Cardioprotective mechanism of ω-3 fatty acid icosapent ethyl (IPE) in cardiomyocytes: role in high glucose and shear stress-induced mechano-transduction dysregulation

doi: 10.1186/s12933-025-03033-8

Figure Lengend Snippet: Meta-analysis of beneficial effects of omega-3 fatty acids in patients with Cardiovascular and cardiometabolic diseases. A Forest plot of CVD patients treated with omega-3 fatty acids or placebo. Data are shown as mean change + SD. The graph was created using Stata software (version 16.0, Stata Corp., College Station, TX). B Forest plot of inflammation markers (IL-6, TNF-α, CRP) in patients with cardiometabolic diseases treated with omega-3 fatty acids or placebo. Data are shown as Mean + SD. The graph was created using Stata software (version 16.0, Stata Corp., College Station, TX)

Article Snippet: Membranes were blocked with 5% milk in TBS-T (Tween 20 buffered with 0.15% Tris pH 8) at room temperature for 1 h and then incubated with the following primary antibodies diluted in TBS-T: p-MST1 (1:1000) (#49,332, Cell signaling Technology), p-LATS1 (1:1000) (#8654, Cell signaling Technology), active YAP (1:1000) (#ab205270, Abcam), TAZ (1:1000) (#83,669, Cell signaling Technology), Oxidative stress defense western blot cocktail (1:1000) (# ab179843 Catalase, SOD1, TRX, Abcam), p-NF-kB (1:1000) (E-AB-68092, ELAB Science), IL-6 (1:1000) (E-AB-30095, Elabscience), SOD2 (1:1000) (#ab13533, Abcam), p-AMPK (1:1000) (E-AB-21121, ELAB Science), PPAR-α (1:1000) (#ab227074, Abcam), PPAR-γ (1:1000) (#2443, Cell signaling Technology), Integrin β3 (1:1000) (#13,166, Cell signaling Technology), Bcl-2 (1:1000) (E-AB-15522, ELAB Science), Bax (1:1000) (E-AB-22128, ELAB Science) overnight at 4°C.

Techniques: Software

Journal: Cardiovascular Diabetology

Article Title: Cardioprotective mechanism of ω-3 fatty acid icosapent ethyl (IPE) in cardiomyocytes: role in high glucose and shear stress-induced mechano-transduction dysregulation

doi: 10.1186/s12933-025-03033-8

Figure Lengend Snippet: IPE effects on inflammation and oxidative stress in a dynamic shear stress model of cardiomyocytes. A , B , E qRT-PCR for NF-kB ( A , left panel), IL-6 ( B , left panel), SOD2 ( E , left panel) in AC16 cells exposed to NG, NG FLOW, NG FLOW + IPE. β-Actin was used as internal control. The fold increase of mRNA expression compared with NG was calculated using the 2 −ΔΔCt method. Data are mean ± SEM. * P < 0.05 vs NG; ** P < 0.05 vs NG FLOW. Western blot for p-NF-kB ( A , right panel), IL-6 ( B , right panel), Catalase ( D ), SOD2 ( E , right panel) in cardiomyocytes AC16 in NG, NG FLOW, NG FLOW + IPE. The histograms show the densitometric analysis of 3 separate experiments representing the relative expression; NG value was set as 1. * P < 0.05 vs NG; ** P < 0.05 vs NG FLOW

Article Snippet: Membranes were blocked with 5% milk in TBS-T (Tween 20 buffered with 0.15% Tris pH 8) at room temperature for 1 h and then incubated with the following primary antibodies diluted in TBS-T: p-MST1 (1:1000) (#49,332, Cell signaling Technology), p-LATS1 (1:1000) (#8654, Cell signaling Technology), active YAP (1:1000) (#ab205270, Abcam), TAZ (1:1000) (#83,669, Cell signaling Technology), Oxidative stress defense western blot cocktail (1:1000) (# ab179843 Catalase, SOD1, TRX, Abcam), p-NF-kB (1:1000) (E-AB-68092, ELAB Science), IL-6 (1:1000) (E-AB-30095, Elabscience), SOD2 (1:1000) (#ab13533, Abcam), p-AMPK (1:1000) (E-AB-21121, ELAB Science), PPAR-α (1:1000) (#ab227074, Abcam), PPAR-γ (1:1000) (#2443, Cell signaling Technology), Integrin β3 (1:1000) (#13,166, Cell signaling Technology), Bcl-2 (1:1000) (E-AB-15522, ELAB Science), Bax (1:1000) (E-AB-22128, ELAB Science) overnight at 4°C.

Techniques: Shear, Quantitative RT-PCR, Control, Expressing, Western Blot