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Image Search Results
Journal: Journal of autoimmunity
Article Title: Low-dose interleukin-2 fosters a dose-dependent regulatory T cell tuned milieu in T1D patients
doi: 10.1016/j.jaut.2015.01.001
Figure Lengend Snippet: Curves represent changes in Treg activation markers. Percentage of CD25highCD127− CD45RA− Tregs among CD4+ cells (A). CD25 MFI (B), percentage of GITR+ (C), CTLA-4+ (D) and basal pSTAT5 expression (E) in CD4+CD25highCD127−Foxp3+Tregs. Data are shown as mean ± SEM of patients’ values normalized by individual baseline values at different time points (the grey zone represents the 5 days IL-2 treatment). Results are for all 6 patients per group, except for pSTAT5 measurement for which 2, 3, 3 and 3 patients were studied for the 0, .33, 1 and 3 MIU doses, respectively.
Article Snippet: Monoclonal antibodies (mAbs) used were CD3-APCa750, CD4-ECD, CD8-APCa700, CD16-FITC, CD19-ECD, CD45RO-FITC, CD56-PCy7 and CD152-PE from Beckman Coulter; CD25-PE,
Techniques: Activation Assay, Expressing
Journal: Journal of autoimmunity
Article Title: Low-dose interleukin-2 fosters a dose-dependent regulatory T cell tuned milieu in T1D patients
doi: 10.1016/j.jaut.2015.01.001
Figure Lengend Snippet: PBMC from controls (n=4) or patients treated with 0.33 MIU (n=3), 1 MIU (n=2), and 3 MIU (n=3) of IL-2 were stimulated with IL-2 for 15 minutes and the activation of pSTAT5 was determined for CD4+ Foxp3+ Tregs and CD4+ Foxp3− CD45RA− TEM cells. (A) Dose response curves and (B) non linear regression analyses of the binding data for the indicated cell populations. The numbers on the regression curves are the EC50s.
Article Snippet: Monoclonal antibodies (mAbs) used were CD3-APCa750, CD4-ECD, CD8-APCa700, CD16-FITC, CD19-ECD, CD45RO-FITC, CD56-PCy7 and CD152-PE from Beckman Coulter; CD25-PE,
Techniques: Activation Assay, Binding Assay