Journal: Molecular medicine reports

Article Title: Isolation and characterization of minipig perivascular stem cells for bone tissue engineering.

doi: 10.3892/mmr.2018.9410

Figure Lengend Snippet: Figure 1. Stromal vascular fraction cell clustering from human liposuction by fluorescence activated cell sorting. (A and B) Viable cells and single cells were identified by their front side detection data through their physical properties. (C) Excluding hematopoietic cells via the expression of CD45. (D) Identification of pericytes and adventitial cells in the CD45‑ group based on CD34‑CD146+ and CD34+CD146‑. Different colors indicated different cell populations. P1, red; P2, blue; P3, green. The pink P4 indicates the CD45‑CD34‑CD146+ perithelial cell phenotype; The yellow P5 indicates the CD45‑CD34+CD146‑ adventitial cells phenotype. CD, cluster of differentiation; SSC, side‑scattered; FSC, forward‑scattered.

Article Snippet: Mp PSC antibodies were as follows: CD146-f luorescein isothiocyanate (FITC; 1:100; cat. no. MCA2141F; Bio-Rad Laboratories, Inc., Hercules, CA, USA), CD45-phycoerythrin (PE; 1:100; cat. no. SM563R; OriGene Technologies, Inc., Rockville, MD, USA) and CD34 (1:100; cat. no. ab81289; Abcam, Cambridge, MA, USA).

Techniques: Fluorescence, FACS, Expressing

Journal: Molecular medicine reports

Article Title: Isolation and characterization of minipig perivascular stem cells for bone tissue engineering.

doi: 10.3892/mmr.2018.9410

Figure Lengend Snippet: Figure 2. Minipig stromal vascular fraction cell clustering by fluorescence activated cell sorting. (A and B) Viable cells and single cells were identified by their front side detection data through their physical properties. (C) Excluding hematopoietic cells via the expression of CD45. (D) identification of pericytes and adventitial cells in the CD45‑ group based on CD34‑CD146+ and CD34+CD146‑. P1, red; P2, blue; P3, pink; P4, green; P5, light blue. CD, cluster of differentia tion; SSC, side‑scattered; FSC, forward‑scattered.

Article Snippet: Mp PSC antibodies were as follows: CD146-f luorescein isothiocyanate (FITC; 1:100; cat. no. MCA2141F; Bio-Rad Laboratories, Inc., Hercules, CA, USA), CD45-phycoerythrin (PE; 1:100; cat. no. SM563R; OriGene Technologies, Inc., Rockville, MD, USA) and CD34 (1:100; cat. no. ab81289; Abcam, Cambridge, MA, USA).

Techniques: Fluorescence, FACS, Expressing