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Image Search Results
Journal: Journal of Histochemistry and Cytochemistry
Article Title: Periarteriolar Glioblastoma Stem Cell Niches Express Bone Marrow Hematopoietic Stem Cell Niche Proteins
doi: 10.1369/0022155417749174
Figure Lengend Snippet: Details of Primary Antibodies Used for Chromogenic Immunohistochemistry.
Article Snippet:
Techniques: Immunohistochemistry, Marker, Expressing, Mutagenesis
Journal: Journal of Histochemistry and Cytochemistry
Article Title: Periarteriolar Glioblastoma Stem Cell Niches Express Bone Marrow Hematopoietic Stem Cell Niche Proteins
doi: 10.1369/0022155417749174
Figure Lengend Snippet: (A) Immunohistochemical localization of CXCR4 (A, B), SDF-1α (C, D), CatK (E, F), OPN (G–J), and CD44 (K, L) in serial cryostat sections of human glioblastoma. (A, B) CXCR4 expression was present in a cellular rim around a large arteriole (arrows) and in some endothelial cells (arrow head). (C, D). Strong SDF-1α expression was found in a thin rim around a large arteriole (arrows) and in endothelial cells (arrow head). (E, F). Strong CatK expression was found in a thin rim around a large arteriole (arrows) and low expression of CatK was found in endothelial cells (arrow head). (G–J). OPN expression was present in a thin rim around a large arteriole (arrows), in endothelial cells and cells in the arteriolar wall (arrow heads). (K, L) CD44 expression was found all over the section, but the positivity was strongest in a cellular rim around a large arteriole (arrows). A, C, E, G, I, K: bar = 40 µm; B, D, F, H, J, L: bar = 20 µm. Abbreviations: CXCR4, C-X-C chemokine receptor type 4; SDF-1α, stromal cell–derived factor-1α; CatK, cathepsin K; OPN, osteopontin.
Article Snippet:
Techniques: Immunohistochemical staining, Expressing, Derivative Assay
Journal: Journal of Histochemistry and Cytochemistry
Article Title: Periarteriolar Glioblastoma Stem Cell Niches Express Bone Marrow Hematopoietic Stem Cell Niche Proteins
doi: 10.1369/0022155417749174
Figure Lengend Snippet: Overview of the similarities between GSC niches in glioblastoma and HSC niches in bone marrow. (A) Hematoxylin and eosin staining of human glioblastoma tissue sections showing a GSC niche around an arteriole (yellow rectangle). 1, lumen of arteriole; 2, endothelial cells; 3, smooth muscle cells; 4, arteriolar adventitia; 5, GSCs; 6, glioblastoma cells; 7, venule. Scale bar = 40 µm. (B) Schematic representation of the GSC niche showing that SDF-1α/CXCR4 interactions and OPN/CD44 interactions at the arteriolar adventitia retain GSCs in the GSC niche and that CatK activity releases GSCs out of the niche, so that GSCs differentiate into glioblastoma progenitor cells. The numbers in the illustration correspond with the numbers in 8A. (C). Giemsa staining of murine bone marrow tissue, showing an HSC niche around an arteriole (yellow rectangle). I, lumen of arteriole; II, endothelial cells; III, smooth muscle cells of the arteriolar tunica media; IV, arteriolar adventitia; V, HSCs; VI, hematopoietic progenitor cells; VII, sinusoid; VIII, bone; IX, osteoblast; X, osteoclast. Scale bar = 60 µm. (D). Schematic representation of the HSC niche showing that SDF-1α/CXCR4 interactions and OPN/CD44 interactions at the arteriolar adventitia retain HSCs in the HSC niche and that CatK activity releases HSCs out of the niche, so that HSCs differentiate into hematopoietic progenitor cells in vicinity of sinusoids and subsequently migrate out of the HSC niche into the circulation. The numbers in the illustration correspond with the numbers in 8C. Abbreviations: GSC, glioblastoma stem cell; HSC, hematopoietic stem cell; SDF-1α, stromal cell–derived factor-1α; CXCR4, C-X-C chemokine receptor type 4; OPN, osteopontin; CatK, cathepsin K.
Article Snippet:
Techniques: Staining, Activity Assay, Derivative Assay