Journal: NPG Asia Materials

Article Title: Spatiotemporal regulation of endogenous MSCs using a functional injectable hydrogel system for cartilage regeneration

doi: 10.1038/s41427-021-00339-3

Figure Lengend Snippet: Fig. 10 MSC homing in cartilage defects at 7 days after the operation. A Immunofluorescent staining for CD44 and CD90. B, C Quantitative results for CD44- and CD90-positive cells.

Article Snippet: At 1 week postoperatively, samples were assessed by immunofluorescence staining for CD44 (1:100, NBP222530F, Novus) and CD90 (1:100, ab225, Abcam) to identify endogenous MSC recruitment.

Techniques: Staining

Journal: Cancers

Article Title: Overexpression of Cystine/Glutamate Antiporter xCT Correlates with Nutrient Flexibility and ZEB1 Expression in Highly Clonogenic Glioblastoma Stem-like Cells (GSCs)

doi: 10.3390/cancers13236001

Figure Lengend Snippet: High Gln/Glu ratios predict ZEB1 and xCT expression in primary GBM tumors. ( a ) Expanded regions from 1 H-NMR spectra showing Gln and Glu multiplets of metabolic extracts from seven primary glioblastoma (pGBM) patient samples. Spectra were normalized to the Glu content to visualize the differences in Gln/Glu ratios. ( b ) Immunoblotting of ZEB1, xCT, CD133, CD44, and cMYC protein in GBM patient samples arranged according to their Gln/Glu ratios in ascending order (loading control = β actin). A non-neoplastic brain sample of a trauma patient was blotted as a control. Abbreviations: ctrl, control; Gln, glutamine; Glu, glutamate; n.d., not detectable; pGBM, primary glioblastoma; ppm, parts per million. correspond to b.

Article Snippet: We applied the following primary antibodies and used the indicated dilutions: ZEB1 (1:2000, Sigma, #HPA027524), CD133 (1:250, Miltenyi Biotec, Bergisch Gladbach, Germany, #W6B3C1), c-Myc (1:1000, Thermo Fisher Scientific, #9E10), SOX2 (1:1000, Cell Signaling Technology, Cambridge, UK, #L1D6A2), xCT (1:1000, Thermo Fisher Scientific, #PA1-16893 and 1:1000, Cell Signaling Technologies, #12691), CD44 (1:2000, R&D Systems, Minneapolis, MN, USA, #AF3660), and β-actin (Thermo Fisher Scientific, #MA5-15739 and Cell Signaling Technology, #4970) were incubated overnight at 4 °C in 5% milk powder in Tris-buffered saline + 0.1% Tween-20 (TBST).

Techniques: Expressing, Western Blot, Control

Journal: The Journal of Experimental Medicine

Article Title: Peroxisome proliferator–activated receptor δ limits the expansion of pathogenic Th cells during central nervous system autoimmunity

doi: 10.1084/jem.20091663

Figure Lengend Snippet: CD4 + T cells from PPAR-δ −/− mice are hyperresponsive. Naive CD4 + (a) or CD19 + (b) cells were isolated from the spleens of WT or PPAR-δ −/− mice and were stimulated as indicated. Proliferation of these cells was measured using a [ 3 H]-thymidine incorporation assay. Values are means + SEM of triplicate cultures. (c and d) Splenocytes were harvested from nonimmunized WT or PPAR-δ −/− mice. The frequency of CD4 + cells that displayed a memory (CD44 high and CD62L low ; c) or activated (CD69 + or CD25 + ; d) phenotype in each group was determined. In d, values represent mean + SEM frequencies of n = 3–4 mice/group. Data in a–d are representative of two experiments. *, significantly different from WT.

Article Snippet: Antibodies directed against the following mouse cell surface antigens were used: CD4, CD8, CD19, CD11b, CD11c, CD44, CD45, CD62L, and F4/80 antigen (AbD Serotec).

Techniques: Isolation, Thymidine Incorporation Assay

Journal: Turkish Neurosurgery

Article Title: The relationship between the hypoxic process and cancer stem cells in meningioma

doi: 10.5137/1019-5149.jtn.27546-19.2

Figure Lengend Snippet: Figure 1: Cytoplasmic and nuclear staining of HIF1α, CD133, and CD44 antibodies. HIF1α (A-F); A: Strong nuclear staining, B: Moderate nuclear staining, C: Weak nuclear staining, D: Strong cytoplasmic staining, E: Moderate cytoplasmic staining, F: Weak cytoplasmic staining. CD133 (G-M); G: Strong cytoplasmic staining, H: Moderate cytoplasmic staining, I: Weak cytoplasmic staining, K: Strong nuclear staining, L: Moderate nuclear staining, M: Weak nuclear staining. CD44 (N-P); N: Strong cytoplasmic staining, O: Moderate cytoplasmic staining, P: Weak cytoplasmic staining.

Article Snippet: An immunohistochemical study was performed with HIF1α polyclonal antibody (1:200; Elabscience, USA), CD44 monoclonal antibody (1:500; Elabscience, USA), and CD133 polyclonal antibody (1:200; Elabscience, USA) using Ventana’s BenchMark Ultra automated system.

Techniques: Staining

Journal: Turkish Neurosurgery

Article Title: The relationship between the hypoxic process and cancer stem cells in meningioma

doi: 10.5137/1019-5149.jtn.27546-19.2

Figure Lengend Snippet: Figure 2: Mean H scores for cytoplasmic and nuclear staining of HIF1α, CD133, CD44 antibodies and comparative with grade.

Article Snippet: An immunohistochemical study was performed with HIF1α polyclonal antibody (1:200; Elabscience, USA), CD44 monoclonal antibody (1:500; Elabscience, USA), and CD133 polyclonal antibody (1:200; Elabscience, USA) using Ventana’s BenchMark Ultra automated system.

Techniques: Staining

Journal: Turkish Neurosurgery

Article Title: The relationship between the hypoxic process and cancer stem cells in meningioma

doi: 10.5137/1019-5149.jtn.27546-19.2

Figure Lengend Snippet: Figure 3: Comperative distribution of cases according to H score threshold values (HIF1α nuclear, CD133 cytoplasmic- nuclear, CD44 cytoplasmic) and grades.

Article Snippet: An immunohistochemical study was performed with HIF1α polyclonal antibody (1:200; Elabscience, USA), CD44 monoclonal antibody (1:500; Elabscience, USA), and CD133 polyclonal antibody (1:200; Elabscience, USA) using Ventana’s BenchMark Ultra automated system.

Techniques:

Journal: Turkish Neurosurgery

Article Title: The relationship between the hypoxic process and cancer stem cells in meningioma

doi: 10.5137/1019-5149.jtn.27546-19.2

Figure Lengend Snippet: Figure 5: Distribution of cases with high HIF1α nuclear H scores according to the H score threshold values of CD133 cytoplasmic- nuclear and CD44 cytoplasmic staining.

Article Snippet: An immunohistochemical study was performed with HIF1α polyclonal antibody (1:200; Elabscience, USA), CD44 monoclonal antibody (1:500; Elabscience, USA), and CD133 polyclonal antibody (1:200; Elabscience, USA) using Ventana’s BenchMark Ultra automated system.

Techniques: Staining