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Image Search Results
Journal: Journal of the American Heart Association
Article Title: Impact of NKG2D Signaling on Natural Killer and T‐Cell Function in Cerebral Ischemia
doi: 10.1161/jaha.122.029529
Figure Lengend Snippet: Figure 5. Distribution of NKG2D-expressing immune cells and their interaction with ligand-expressing brain resident cells in human stroke patients. A, Representative immunofluorescence staining of NKG2D receptor as well as CD3+ T cells, CD4+ and CD8+ T cell subsets, CD56+ NK cells, and CD56+/CD3+ NKT cells in the brain of human patients with stroke and control patients. B, Representative immunofluorescence staining of NKG2D ligands (ULBP1, −3, and − 4) as well as NeuN+ neurons, CX3CR1+ microglia, CD68+ monocytes/microglia, and glial fibrillary acidic protein (GFAP+) astrocytes in control brain tissue and stroke lesions. ULBP: cytomegalovirus UL16-binding protein. NK indicates natural killer cell; and NKT, natural killer T cell.
Article Snippet: In the degranulation assay, the following fluorescently labeled antibodies purchased from
Techniques: Expressing, Immunofluorescence, Staining, Control, Binding Assay
Journal: Journal for Immunotherapy of Cancer
Article Title: Expression of VISTA regulated via IFN-γ governs endogenous T-cell function and exhibits correlation with the efficacy of CD19 CAR-T cell treated B-malignant mice
doi: 10.1136/jitc-2023-008364
Figure Lengend Snippet: CAR-T cell-induced tumor lysis was not sufficient to reduce VISTA expression on syngeneic activated T cell. (A) Gating strategy of flow cytometry. (B) Percentages of B-ALL cell death in vitro. B-ALL cell alone or co-cultured with purified T-cell stimulated with anti-CD3 and anti-CD28 (T activated ) in vitro were used as the control and T-cell groups, respectively. (C) The production of cytokines was evaluated in the co-culture cellular system for 6 hours. Naïve T cells were included as a control group without antibody activation (T unactivated ). (D) The representative flow cytometry histograms of VISTA levels. The T cells lacking CAR chimerism in the CAR-T groups were designated as GFP − T cells. Three independent experiments were repeated. B-ALL, B acute lymphoblastic leukemia; CAR, chimeric antigen receptor; FMO, fluorescence minus one; GFP, green fluorescent protein; VISTA, V-domain Ig suppressor of T-cell activation.
Article Snippet: Prior to flow cytometry analysis, staining was performed using the following monoclonal antibodies: CD3 FITC (fluorescein isothiocyanate) (clone17A2; BioLegend), CD3 BV650 (clone17A2; BioLegend),
Techniques: Lysis, Expressing, Flow Cytometry, In Vitro, Cell Culture, Purification, Control, Co-Culture Assay, Activation Assay, Fluorescence