cd3ε Search Results


95
Miltenyi Biotec cd3ε microbead kit
Cd3ε Microbead Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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94
Multi Sciences (Lianke) Biotech Co Ltd anti mouse cd3
Anti Mouse Cd3, supplied by Multi Sciences (Lianke) Biotech Co Ltd, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Bio X Cell anti mouse cd3e
Anti Mouse Cd3e, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Bio X Cell anti cd3
Anti Cd3, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Bio X Cell soluble anticd28 pv 1
Soluble Anticd28 Pv 1, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Miltenyi Biotec cd3ε apc
Cd3ε Apc, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Miltenyi Biotec cd3
Figure 5. Distribution of NKG2D-expressing immune cells and their interaction with ligand-expressing brain resident cells in human stroke patients. A, Representative immunofluorescence staining of NKG2D receptor as well as <t>CD3+</t> T cells, CD4+ and CD8+ T cell subsets, CD56+ NK cells, and <t>CD56+/CD3+</t> NKT cells in the brain of human patients with stroke and control patients. B, Representative immunofluorescence staining of NKG2D ligands (ULBP1, −3, and − 4) as well as NeuN+ neurons, CX3CR1+ microglia, CD68+ monocytes/microglia, and glial fibrillary acidic protein (GFAP+) astrocytes in control brain tissue and stroke lesions. ULBP: cytomegalovirus UL16-binding protein. NK indicates natural killer cell; and NKT, natural killer T cell.
Cd3, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Miltenyi Biotec cd3e biotin
Figure 5. Distribution of NKG2D-expressing immune cells and their interaction with ligand-expressing brain resident cells in human stroke patients. A, Representative immunofluorescence staining of NKG2D receptor as well as <t>CD3+</t> T cells, CD4+ and CD8+ T cell subsets, CD56+ NK cells, and <t>CD56+/CD3+</t> NKT cells in the brain of human patients with stroke and control patients. B, Representative immunofluorescence staining of NKG2D ligands (ULBP1, −3, and − 4) as well as NeuN+ neurons, CX3CR1+ microglia, CD68+ monocytes/microglia, and glial fibrillary acidic protein (GFAP+) astrocytes in control brain tissue and stroke lesions. ULBP: cytomegalovirus UL16-binding protein. NK indicates natural killer cell; and NKT, natural killer T cell.
Cd3e Biotin, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Elabscience Biotechnology cd3ε pe cy5
CAR-T cell-induced tumor lysis was not sufficient to reduce VISTA expression on syngeneic activated T cell. (A) Gating strategy of flow cytometry. (B) Percentages of B-ALL cell death in vitro. B-ALL cell alone or co-cultured with purified T-cell stimulated with anti-CD3 and anti-CD28 (T activated ) in vitro were used as the control and T-cell groups, respectively. (C) The production of cytokines was evaluated in the co-culture cellular system for 6 hours. Naïve T cells were included as a control group without antibody activation (T unactivated ). (D) The representative flow cytometry histograms of VISTA levels. The T cells lacking CAR chimerism in the CAR-T groups were designated as GFP − T cells. Three independent experiments were repeated. B-ALL, B acute lymphoblastic leukemia; CAR, chimeric antigen receptor; FMO, fluorescence minus one; GFP, green fluorescent protein; VISTA, V-domain Ig suppressor of T-cell activation.
Cd3ε Pe Cy5, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Elabscience Biotechnology anti cd3ε percp cyanine5 5
CAR-T cell-induced tumor lysis was not sufficient to reduce VISTA expression on syngeneic activated T cell. (A) Gating strategy of flow cytometry. (B) Percentages of B-ALL cell death in vitro. B-ALL cell alone or co-cultured with purified T-cell stimulated with anti-CD3 and anti-CD28 (T activated ) in vitro were used as the control and T-cell groups, respectively. (C) The production of cytokines was evaluated in the co-culture cellular system for 6 hours. Naïve T cells were included as a control group without antibody activation (T unactivated ). (D) The representative flow cytometry histograms of VISTA levels. The T cells lacking CAR chimerism in the CAR-T groups were designated as GFP − T cells. Three independent experiments were repeated. B-ALL, B acute lymphoblastic leukemia; CAR, chimeric antigen receptor; FMO, fluorescence minus one; GFP, green fluorescent protein; VISTA, V-domain Ig suppressor of T-cell activation.
Anti Cd3ε Percp Cyanine5 5, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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93
Bio X Cell anti mouse cd3ε f ab 2 fragments
CAR-T cell-induced tumor lysis was not sufficient to reduce VISTA expression on syngeneic activated T cell. (A) Gating strategy of flow cytometry. (B) Percentages of B-ALL cell death in vitro. B-ALL cell alone or co-cultured with purified T-cell stimulated with anti-CD3 and anti-CD28 (T activated ) in vitro were used as the control and T-cell groups, respectively. (C) The production of cytokines was evaluated in the co-culture cellular system for 6 hours. Naïve T cells were included as a control group without antibody activation (T unactivated ). (D) The representative flow cytometry histograms of VISTA levels. The T cells lacking CAR chimerism in the CAR-T groups were designated as GFP − T cells. Three independent experiments were repeated. B-ALL, B acute lymphoblastic leukemia; CAR, chimeric antigen receptor; FMO, fluorescence minus one; GFP, green fluorescent protein; VISTA, V-domain Ig suppressor of T-cell activation.
Anti Mouse Cd3ε F Ab 2 Fragments, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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94
Proteintech anti mouse cd3a
CAR-T cell-induced tumor lysis was not sufficient to reduce VISTA expression on syngeneic activated T cell. (A) Gating strategy of flow cytometry. (B) Percentages of B-ALL cell death in vitro. B-ALL cell alone or co-cultured with purified T-cell stimulated with anti-CD3 and anti-CD28 (T activated ) in vitro were used as the control and T-cell groups, respectively. (C) The production of cytokines was evaluated in the co-culture cellular system for 6 hours. Naïve T cells were included as a control group without antibody activation (T unactivated ). (D) The representative flow cytometry histograms of VISTA levels. The T cells lacking CAR chimerism in the CAR-T groups were designated as GFP − T cells. Three independent experiments were repeated. B-ALL, B acute lymphoblastic leukemia; CAR, chimeric antigen receptor; FMO, fluorescence minus one; GFP, green fluorescent protein; VISTA, V-domain Ig suppressor of T-cell activation.
Anti Mouse Cd3a, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Figure 5. Distribution of NKG2D-expressing immune cells and their interaction with ligand-expressing brain resident cells in human stroke patients. A, Representative immunofluorescence staining of NKG2D receptor as well as CD3+ T cells, CD4+ and CD8+ T cell subsets, CD56+ NK cells, and CD56+/CD3+ NKT cells in the brain of human patients with stroke and control patients. B, Representative immunofluorescence staining of NKG2D ligands (ULBP1, −3, and − 4) as well as NeuN+ neurons, CX3CR1+ microglia, CD68+ monocytes/microglia, and glial fibrillary acidic protein (GFAP+) astrocytes in control brain tissue and stroke lesions. ULBP: cytomegalovirus UL16-binding protein. NK indicates natural killer cell; and NKT, natural killer T cell.

Journal: Journal of the American Heart Association

Article Title: Impact of NKG2D Signaling on Natural Killer and T‐Cell Function in Cerebral Ischemia

doi: 10.1161/jaha.122.029529

Figure Lengend Snippet: Figure 5. Distribution of NKG2D-expressing immune cells and their interaction with ligand-expressing brain resident cells in human stroke patients. A, Representative immunofluorescence staining of NKG2D receptor as well as CD3+ T cells, CD4+ and CD8+ T cell subsets, CD56+ NK cells, and CD56+/CD3+ NKT cells in the brain of human patients with stroke and control patients. B, Representative immunofluorescence staining of NKG2D ligands (ULBP1, −3, and − 4) as well as NeuN+ neurons, CX3CR1+ microglia, CD68+ monocytes/microglia, and glial fibrillary acidic protein (GFAP+) astrocytes in control brain tissue and stroke lesions. ULBP: cytomegalovirus UL16-binding protein. NK indicates natural killer cell; and NKT, natural killer T cell.

Article Snippet: In the degranulation assay, the following fluorescently labeled antibodies purchased from Miltenyi Biotec were used: CD3 (17A2, Cat. 130-118-849), CD45 (REA737, Cat. 130-110-803), CD8a (REA601, Cat. 130-120-822), NKG2D (CX5, Cat. 130-102-730), CD107a (REA777, Cat. 130-111-505), CD161 (REA1162, Cat. 130-120-510).

Techniques: Expressing, Immunofluorescence, Staining, Control, Binding Assay

CAR-T cell-induced tumor lysis was not sufficient to reduce VISTA expression on syngeneic activated T cell. (A) Gating strategy of flow cytometry. (B) Percentages of B-ALL cell death in vitro. B-ALL cell alone or co-cultured with purified T-cell stimulated with anti-CD3 and anti-CD28 (T activated ) in vitro were used as the control and T-cell groups, respectively. (C) The production of cytokines was evaluated in the co-culture cellular system for 6 hours. Naïve T cells were included as a control group without antibody activation (T unactivated ). (D) The representative flow cytometry histograms of VISTA levels. The T cells lacking CAR chimerism in the CAR-T groups were designated as GFP − T cells. Three independent experiments were repeated. B-ALL, B acute lymphoblastic leukemia; CAR, chimeric antigen receptor; FMO, fluorescence minus one; GFP, green fluorescent protein; VISTA, V-domain Ig suppressor of T-cell activation.

Journal: Journal for Immunotherapy of Cancer

Article Title: Expression of VISTA regulated via IFN-γ governs endogenous T-cell function and exhibits correlation with the efficacy of CD19 CAR-T cell treated B-malignant mice

doi: 10.1136/jitc-2023-008364

Figure Lengend Snippet: CAR-T cell-induced tumor lysis was not sufficient to reduce VISTA expression on syngeneic activated T cell. (A) Gating strategy of flow cytometry. (B) Percentages of B-ALL cell death in vitro. B-ALL cell alone or co-cultured with purified T-cell stimulated with anti-CD3 and anti-CD28 (T activated ) in vitro were used as the control and T-cell groups, respectively. (C) The production of cytokines was evaluated in the co-culture cellular system for 6 hours. Naïve T cells were included as a control group without antibody activation (T unactivated ). (D) The representative flow cytometry histograms of VISTA levels. The T cells lacking CAR chimerism in the CAR-T groups were designated as GFP − T cells. Three independent experiments were repeated. B-ALL, B acute lymphoblastic leukemia; CAR, chimeric antigen receptor; FMO, fluorescence minus one; GFP, green fluorescent protein; VISTA, V-domain Ig suppressor of T-cell activation.

Article Snippet: Prior to flow cytometry analysis, staining was performed using the following monoclonal antibodies: CD3 FITC (fluorescein isothiocyanate) (clone17A2; BioLegend), CD3 BV650 (clone17A2; BioLegend), CD3ε PE/Cy5 (clone145-2C11; Elabscience), CD4 PE/Cyanine7 (cloneGK1.5; BioLegend), CD4 Alex Flour700 (cloneRM4-5; BioLegend); CD8a APC (allophycocyanin) (clone53-6.7; BioLegend), CD8 APC/Cyanine7 (clone53-6.7; BioLegend); CD11b PB (Pacific Blue) (cloneM1/70; BioLegend), F4/80 BV605 (cloneBM8; BioLegend), MHC-II AF700 (cloneM5/114.15.2; BioLegend), CD19 PE-cf594 (clone6D5; BioLegend), CD19 BV510 (clone6D5; BioLegend), CD11c PE-cf594 (cloneN418; BioLegend), IFN-γ PE/Cyanine7 (cloneXMG1.2; BioLegend), TNF-α APC (cloneMP6-XT22; BioLegend); Granzyme B eFlour450 (cloneM5/114.15.2; Thermo Fisher Scientific); CD62L PB (cloneMEL-1; BD Biosciences), CD44 APC/eFlour780 (cloneIM7; BD Biosciences); PD-1 PE/Cyanine7 (clone RMP1-30; BioLegend), TIGIT (Vstm3) PE (clone1G9; BioLegend); NGFR (CD271) APC (cloneME204; BioLegend), VISTA (PD-1H) PE (cloneMIH63; BioLegend), CD69 BV510 (cloneH1.2F3; BioLegend), and CD20 APC/Cyanine7 (clone SA275A11; BioLegend).

Techniques: Lysis, Expressing, Flow Cytometry, In Vitro, Cell Culture, Purification, Control, Co-Culture Assay, Activation Assay, Fluorescence