Journal: Journal of Traditional Chinese Medicine

Article Title: Saponin Ⅰ from Shuitianqi ( Rhizoma Schizocapasae Plantagineae ) inhibits metastasis by negatively regulating the transforming growth factor-β1/Smad7 network and epithelial-mesenchymal transition in the intrahepatic metastasis Bagg’s Albino/c mouse model

doi: 10.19852/j.cnki.jtcm.2024.04.006

Figure Lengend Snippet: A: Western blotting was used to detect the expression of CD31, CD34 and VEGF proteins. A1: model group; A2: cisplatin group; A3: SSPH Ⅰ (25 mg/kg) group; A4: SSPH Ⅰ (50 mg/kg) group; A5: SSPH Ⅰ (75 mg/kg) group. B: quantitative analysis of CD31, CD34 and VEGF proteins expression. C: immunohistochemical assay was used to detect the expression of CD31 protein in model group, cisplatin group and SSPH Ⅰ groups. C1: model group; C2: cisplatin group; C3: SSPH Ⅰ (25 mg/kg) group; C4: SSPH Ⅰ (50 mg/kg) group; C5: SSPH Ⅰ (75 mg/kg) group. magnification at ×200; scale bar, 20 μm, dyeing method of all pictures are the immunohistochemical DAB method. D: immunohistochemical assay was used to detect the expression of CD34 protein in model group, cisplatin group and SSPH Ⅰ groups. D1: model group; D2: cisplatin group; D3: SSPH Ⅰ (25 mg/kg) group; D4: SSPH Ⅰ (50 mg/kg) group; D5: SSPH Ⅰ (75 mg/kg) group. magnification at ×200; scale bar, 20 μm, dyeing method of all pictures are the immunohistochemical DAB method. E: immunohistochemical assay was used to detect the expression of VEGF protein in model group, cisplatin group and SSPH Ⅰ groups. E1: model group; E2: cisplatin group; E3: SSPH Ⅰ (25 mg/kg) group; E4: SSPH Ⅰ (50 mg/kg) group; E5: SSPH Ⅰ (75 mg/kg) group. magnification at ×200; scale bar, 20 μm, dyeing method of all pictures are the immunohistochemical DAB method. F: quantitative analysis of CD31, CD34 and VEGF proteins expression. G: enzyme linked immunosorbent assay was used to detect the expression of VEGF protein in serum. Model group: free diet + gavage with equal amount of normal saline per day; cisplatin group: model group + intraperitoneal injection with equal amount of cisplatin 2 mg/kg every other day; SSPH Ⅰ (25 mg/kg) group: model group + gavage with equal amount of SSPH Ⅰ 25 mg/kg per day; SSPH Ⅰ (50 mg/kg) group: model group + gavage with equal amount of SSPH Ⅰ 50 mg/kg per day; SSPH Ⅰ (75 mg/kg) group: model group + gavage with equal amount of SSPH Ⅰ 75 mg/kg per day. SSPH Ⅰ: Saponin I from Shuitianqi (Rhizoma Schizocapasae Plantagineae); VEGF: vascular endothelial growth factor; DAB: diaminobenzidine. All data was measured by one-way analysis, and Newman-Keuls test was performed for inter-group comparisons. All data was presented as mean ± standard deviation (n = 3). aP < 0.05, bP < 0.001 compared to the model group.

Article Snippet: Subsequently, sections were incubated in 5% BSA blocking fluid for 1 h, with subsequent ON incubation in primary antibodies at 4 ℃: E-cadherin, N-cadherin, Vimentin, TGF-β1(PTG, 21898-1-AP), Smad7, MMP-2, MMP-9, CD31, CD34 (Sino Biological Inc., 50589-T24), and VEGF (all dilution 1:200).

Techniques: Western Blot, Expressing, Immunohistochemical staining, Enzyme-linked Immunosorbent Assay, Saline, Injection, Standard Deviation

Journal: Acta Pharmacologica Sinica

Article Title: Brain protection against ischemic stroke using choline as a new molecular bypass treatment

doi: 10.1038/aps.2015.104

Figure Lengend Snippet: Effects of oral choline treatment on angiogenesis in the ischemic brains. (A) Macroscopic images of the pial vasculature. (B) Microscopic images of ischemic cerebral cortex sections immunohistochemically stained using an anti-CD34 antibody to analyze vessel density, scale bar=20 μm. (C) Vessel density (n=6). (D) Number of microvessels in the cortex (n=3). Mean±SEM. bP<0.05, cP<0.01 vs sham; eP<0.05, fP<0.01 vs pMCAO.

Article Snippet: The capillary density was examined using a primary rabbit anti-rat CD34 antibody (Bioss, Beijing, China).

Techniques: Staining

Journal: Frontiers in Neuroscience

Article Title: Trihydroxyethyl Rutin Provides Neuroprotection in Rats With Cervical Spinal Cord Hemi-Contusion

doi: 10.3389/fnins.2021.759325

Figure Lengend Snippet: CD34 immunohistochemical staining. (A) Sham group; (B) injury group; (C) T50 group; (D) T100 group; (E) statistical results of microvascular density showed that MVD of injury group, T50 group and T100 group were significantly lower than Sham group, and the MVD of T50 group and T100 group were significantly higher than injury group. * P < 0.05, comparison between each group and sham group; # P < 0.05, comparison between T50 group or T100 group and injury group.

Article Snippet: The resulting sections were soaked with 3% hydrogen peroxide to block endogenous peroxidase activity and then incubated overnight with rabbit polyclonal anti-CD34 antibody (1:1,000; Bioworld Technology, St. Louis Park, MN, United States).

Techniques: Immunohistochemical staining, Staining

Journal: Journal of Tissue Engineering and Regenerative Medicine

Article Title: In vivo analysis of vascularization and biocompatibility of electrospun polycaprolactone fibre mats in the rat femur chamber

doi: 10.1002/term.2868

Figure Lengend Snippet: HE staining, van Gieson staining, and immunohistochemical detection of CD 34: Representative histological stainings (a–o) 20 days after implantation into the femur chamber in Lewis rats. (a–c) HE staining of (1) granulation tissue, (2) implant, and (3) bone. (d–f) HE staining with higher magnification. (g–i) Collagen fibres were detected by van Gieson staining. (j–l) The presence of endothelial cells and accordingly the presence of vascular structures were confirmed by immunohistochemical detection of CD34. (m–o) Negative controls. Areas of the implant are marked (#), and arrows denote vascular structures [Colour figure can be viewed at wileyonlinelibrary.com ]

Article Snippet: For detection of capillaries, endothelial cells were immunohistochemically stained using a rabbit anti‐rat CD34 antibody (Hoelzel Diagnostika Handels GmbH, Koeln, Germany).

Techniques: Staining, Immunohistochemical staining