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human cd34 cell nucleofector kit  (Amaxa)
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Amaxa human cd34 cell nucleofector kit
<t>CD133+/CD34+</t> cells isolated from freshly collected human umbilical cord bloods (from five different samples) were expanded on PES-nanofiber scaffolds with a serum-free expansion media containing growth factors. Cells were re-expanded for four more times and fold expansions were shown after each round of culture.
Human Cd34 Cell Nucleofector Kit, supplied by Amaxa, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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CD133+/CD34+ cells isolated from freshly collected human umbilical cord bloods (from five different samples) were expanded on PES-nanofiber scaffolds with a serum-free expansion media containing growth factors. Cells were re-expanded for four more times and fold expansions were shown after each round of culture.

Journal: Biomaterials

Article Title: Retention of stemness and vasculogenic potential of human umbilical cord blood stem cells after repeated expansions on PES-nanofiber matrices

doi: 10.1016/j.biomaterials.2014.06.037

Figure Lengend Snippet: CD133+/CD34+ cells isolated from freshly collected human umbilical cord bloods (from five different samples) were expanded on PES-nanofiber scaffolds with a serum-free expansion media containing growth factors. Cells were re-expanded for four more times and fold expansions were shown after each round of culture.

Article Snippet: Five times nanofiber-expanded stem cells were transfected with either GFP containing vector (pmaxGFP) or VIP vectors (VEGF IRES and PDGF in pAMFG vector) using a human CD34 cell Nucleofector kit (Amaxa Inc.) following manufacturer’s protocol.

Techniques: Isolation

Flowcytometric analysis for the expression levels of CD34, CXCR4, and CD133 molecules after each round of expansion of stem cells on PES-nanofiber scaffold. Three representative samples were showned out of five samples studied.

Journal: Biomaterials

Article Title: Retention of stemness and vasculogenic potential of human umbilical cord blood stem cells after repeated expansions on PES-nanofiber matrices

doi: 10.1016/j.biomaterials.2014.06.037

Figure Lengend Snippet: Flowcytometric analysis for the expression levels of CD34, CXCR4, and CD133 molecules after each round of expansion of stem cells on PES-nanofiber scaffold. Three representative samples were showned out of five samples studied.

Article Snippet: Five times nanofiber-expanded stem cells were transfected with either GFP containing vector (pmaxGFP) or VIP vectors (VEGF IRES and PDGF in pAMFG vector) using a human CD34 cell Nucleofector kit (Amaxa Inc.) following manufacturer’s protocol.

Techniques: Expressing

A. A representative flowcytometric analysis of five-times expanded cells for various markers related to the stem cells. B. Five independent flowcytometric analysis data is graphically presented in a cumulative form.

Journal: Biomaterials

Article Title: Retention of stemness and vasculogenic potential of human umbilical cord blood stem cells after repeated expansions on PES-nanofiber matrices

doi: 10.1016/j.biomaterials.2014.06.037

Figure Lengend Snippet: A. A representative flowcytometric analysis of five-times expanded cells for various markers related to the stem cells. B. Five independent flowcytometric analysis data is graphically presented in a cumulative form.

Article Snippet: Five times nanofiber-expanded stem cells were transfected with either GFP containing vector (pmaxGFP) or VIP vectors (VEGF IRES and PDGF in pAMFG vector) using a human CD34 cell Nucleofector kit (Amaxa Inc.) following manufacturer’s protocol.

Techniques: