Journal: Developmental dynamics : an official publication of the American Association of Anatomists

Article Title: Rodent monocyte-derived macrophages do not express CD163: Comparative analysis using macrophages from living boreoeutherians.

doi: 10.1002/dvdy.70036

Figure Lengend Snippet: FIGURE 9 Immunohistological comparison of CD163 and CD206 expression in various murine tumors. Immunohistochemical staining of CD163 and CD206 in MCA205, MC38, hepatoma, and histiocytic sarcoma. Scale bars: 200 μm.

Article Snippet: The membranes were cut and incubated with the following primary antibodies: anti-CD163 antibodies (clone EPR19518; Abcam) (for all animals), anti-CD204 antibodies (clone MCA1322GA; Bio-Rad) (for mouse), anti-CD206 antibodies (clone MCA2235GA; Bio-Rad) (for mouse), and anti-β-actin antibodies (clone C4; Santa Cruz Biotechnology, Dallas, TX, USA) (for all animals).

Techniques: Comparison, Expressing, Immunohistochemical staining, Staining

Journal: Developmental dynamics : an official publication of the American Association of Anatomists

Article Title: Rodent monocyte-derived macrophages do not express CD163: Comparative analysis using macrophages from living boreoeutherians.

doi: 10.1002/dvdy.70036

Figure Lengend Snippet: FIGURE 11 Expression of macrophage surface markers on recovered macrophages after clodronate liposome-mediated macrophage depletion. (A) CCL2 gene expression in the liver and spleen 3 days and 1 month post-clodronate administration in C57BL/6 mice. Data presented as mean ± SD. **, p < .01. (B) Western blot analyses of CD163, CD204, CD206 in the liver and spleen 1 month post-clodronate administration in C57BL/6 mice.

Article Snippet: The membranes were cut and incubated with the following primary antibodies: anti-CD163 antibodies (clone EPR19518; Abcam) (for all animals), anti-CD204 antibodies (clone MCA1322GA; Bio-Rad) (for mouse), anti-CD206 antibodies (clone MCA2235GA; Bio-Rad) (for mouse), and anti-β-actin antibodies (clone C4; Santa Cruz Biotechnology, Dallas, TX, USA) (for all animals).

Techniques: Expressing, Gene Expression, Western Blot

Journal: Developmental dynamics : an official publication of the American Association of Anatomists

Article Title: Rodent monocyte-derived macrophages do not express CD163: Comparative analysis using macrophages from living boreoeutherians.

doi: 10.1002/dvdy.70036

Figure Lengend Snippet: FIGURE 13 Expression of macrophage surface markers in the liver and spleen of obese mice. (A) Gene expression of CCL2, TNF-α, and IL-6 in the liver and spleen 3 months post-ND or HFD administration. Data presented as mean ± SD. *, p < .05. **, p < .01. (B) Western blot analyses of CD163, CD204, CD206 in the liver and spleen 3 months post-ND or HFD administration.

Article Snippet: The membranes were cut and incubated with the following primary antibodies: anti-CD163 antibodies (clone EPR19518; Abcam) (for all animals), anti-CD204 antibodies (clone MCA1322GA; Bio-Rad) (for mouse), anti-CD206 antibodies (clone MCA2235GA; Bio-Rad) (for mouse), and anti-β-actin antibodies (clone C4; Santa Cruz Biotechnology, Dallas, TX, USA) (for all animals).

Techniques: Expressing, Gene Expression, Western Blot

Journal: Cell stress & chaperones

Article Title: COL5A2-mediated endoplasmic reticulum stress promotes macrophage M2 polarization in lung adenocarcinoma.

doi: 10.1016/j.cstres.2025.100081

Figure Lengend Snippet: Fig. 2 COL5A2 mediates ER stress to promote macrophage M2 polarization. (a) GSEA analysis of the signaling pathways enriched by differential expression of COL5A2; (b) CCK-8 assay detected cell viability in various groups; (c) WB assessed the protein expression levels of ER stress-related proteins (GRP78 and CHOP) in cells of various groups; (d) IF of the expression of M2 macrophage marker (CD206) in various groups, scale bar = 100 µm; (e) flow cytometry analysis of the proportion of CD68 and CD206 double-positive macrophages in various groups; (f) qPCR analysis of the mRNA expression levels of TGF-β and IL-10 in various groups. *P < 0.05. Abbreviations used: CCK-8, cell counting kit-8ER; endoplasmic reticulum; IF, immunofluorescence; qPCR, quantitative polymerase chain reaction; WB, Western blot.

Article Snippet: For cellular assays, cells were seeded in 6-well plates, fixed with 4% paraformaldehyde for 20 min, washed, permeabilized with 0.5% Triton-X 100 for 30 min, blocked, and incubated with a CD206 antibody (59414, Cell Signaling Technology, USA) overnight at 4 °C.

Techniques: Protein-Protein interactions, Quantitative Proteomics, CCK-8 Assay, Expressing, Marker, Flow Cytometry, Cell Counting, Immunofluorescence, Real-time Polymerase Chain Reaction, Western Blot

Journal: Cell stress & chaperones

Article Title: COL5A2-mediated endoplasmic reticulum stress promotes macrophage M2 polarization in lung adenocarcinoma.

doi: 10.1016/j.cstres.2025.100081

Figure Lengend Snippet: Fig. 4 COL5A2-mediated ER stress amplifies PD-L1-rich exosome release to enhance macrophage M2 polarization. (a) WB analysis of PD-L1 protein levels in exosomes among different groups; (b) PKH26 staining assessed the uptake of exosomes by macrophages, scale bar = 200 µm; (c) WB analysis of PD-L1 protein levels in macrophages among different groups after exosome uptake by macrophages; (d) IF analyzed the expression of M2 macrophage marker (CD206) among different groups, scale bar = 100 µm; (e) flow cytometry determined the ratio of CD68+ and CD206+ double-positive macrophages among different groups; (f) qPCR measured mRNA levels of TGF-β and IL-10 among different groups. *P < 0.05. Abbreviations used: ER, endoplasmic reticulum; IF, immunofluorescence; qPCR, quantitative polymerase chain reaction; WB, Western blot.

Article Snippet: For cellular assays, cells were seeded in 6-well plates, fixed with 4% paraformaldehyde for 20 min, washed, permeabilized with 0.5% Triton-X 100 for 30 min, blocked, and incubated with a CD206 antibody (59414, Cell Signaling Technology, USA) overnight at 4 °C.

Techniques: Staining, Expressing, Marker, Flow Cytometry, Immunofluorescence, Real-time Polymerase Chain Reaction, Western Blot

Journal: Cell stress & chaperones

Article Title: COL5A2-mediated endoplasmic reticulum stress promotes macrophage M2 polarization in lung adenocarcinoma.

doi: 10.1016/j.cstres.2025.100081

Figure Lengend Snippet: Fig. 5 In vivo impact of COL5A2 on macrophage M2 polarization. (a) and (b) Tracking tumor growth and tumor size; (c) IHC evaluation of cell proliferation marker KI-67, scale bar = 50 µm; (d) WB profiling of COL5A2 and PD-L1 proteins; (e) Immunofluorescence for M2 macrophage marker CD206 expression, scale bar = 50 µm; (f) flow cytometry analysis of CD68+CD206+ macrophage populations; (g) WB detection of ER stress indicators GRP78 and CHOP. *P < 0.05. Abbreviations used: ER, endoplasmic reticulum; IHC, immunohistochemistry.

Article Snippet: For cellular assays, cells were seeded in 6-well plates, fixed with 4% paraformaldehyde for 20 min, washed, permeabilized with 0.5% Triton-X 100 for 30 min, blocked, and incubated with a CD206 antibody (59414, Cell Signaling Technology, USA) overnight at 4 °C.

Techniques: In Vivo, Marker, Immunofluorescence, Expressing, Flow Cytometry, Immunohistochemistry

Journal: Molecular Metabolism

Article Title: Indoleamine 2,3-dioxygenase mediates the therapeutic effects of adipose-derived stromal/stem cells in experimental periodontitis by modulating macrophages through the kynurenine-AhR-NRF2 pathway

doi: 10.1016/j.molmet.2022.101617

Figure Lengend Snippet: ASC therapeutic effects on experimental periodontitis in rats. (A) The schematic diagram showed the induction of the experimental rat model of periodontitis for 21 days and ASC injection after the removal of ligatures. (B) Micro-CT imaging and bone parameters (CEJ-ABC distance, BV/TV, Tb. Th, and Tb. Sp) between healthy and experimental rats of periodontitis. Scale bar, 1 mm. (C) Micro-CT imaging displayed the alveolar bone of PBS-injected and ASC-injected rats on day 7, day 14, and day 21 Scale bar, 1 mm. (D) The CEJ-ABC distance and bone parameters (BV/TV, Tb. Th, and Tb. Sp) between the PBS-injected and ASC-injected rats. (E) Representative immunofluorescence staining of M1 phenotype macrophages (iNOS + ; Green) and M2 macrophages (CD206 + ; Red) in sagittal sections of maxillary molars in PBS-injected and ASC-injected rats. Scale bar, 20 μm. (F) The calculated ratio of iNOS + /CD206 + macrophages in PBS-injected and ASC-injected groups. CEJ-ABC, cementoenamel junction and alveolar bone crest. All data were expressed as mean ± SD; ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001.

Article Snippet: For tissues, the sections were incubated with a mouse anti-CD206 monoclonal antibody (1:100, 60143-1-lg, Proteintech), a rabbit anti-NRF2 monoclonal antibody (1:100, A0674, Abclonal), a rabbit anti-inducible nitric oxide synthase (iNOS) polyclonal antibody (1:100, 18985-1-AP, Proteintech) overnight at 4 °C.

Techniques: Injection, Micro-CT, Imaging, Immunofluorescence, Staining

Journal: Molecular Metabolism

Article Title: Indoleamine 2,3-dioxygenase mediates the therapeutic effects of adipose-derived stromal/stem cells in experimental periodontitis by modulating macrophages through the kynurenine-AhR-NRF2 pathway

doi: 10.1016/j.molmet.2022.101617

Figure Lengend Snippet: ASCs modulated macrophage polarization through NRF2. (A) Representative immunohistochemical staining of NRF2 in the PBS-injected and ASC-injected rats on day 7, day 14, and day 21. Scale bar, 50 μm. (B) Representative immunofluorescence staining of NRF2 (Green) and M2 macrophages (CD206 + ; Red) in sagittal sections of maxillary molars in PBS-injected and ASC-injected rats. Scale bar, 20 μm. (C) Schematic diagram of knocking down NRF2 in macrophages and macrophages cocultured with ASCs and LPS stimulation. (D, E) RT-qPCR and western blotting were used to measure the NRF2 siRNA or siNC in macrophages. (F, G) The mRNA expression and protein levels of M1 markers were increased, while those of M2 markers were decreased after silencing NRF2 in the cocultured groups. R, root; PDL, periodontal ligament; AB, alveolar bone; siRNA, small interfering RNA; NC, negative control. All data were expressed as mean ± SD; ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001.

Article Snippet: For tissues, the sections were incubated with a mouse anti-CD206 monoclonal antibody (1:100, 60143-1-lg, Proteintech), a rabbit anti-NRF2 monoclonal antibody (1:100, A0674, Abclonal), a rabbit anti-inducible nitric oxide synthase (iNOS) polyclonal antibody (1:100, 18985-1-AP, Proteintech) overnight at 4 °C.

Techniques: Immunohistochemical staining, Staining, Injection, Immunofluorescence, Quantitative RT-PCR, Western Blot, Expressing, Small Interfering RNA, Negative Control

Journal: Molecular Metabolism

Article Title: Indoleamine 2,3-dioxygenase mediates the therapeutic effects of adipose-derived stromal/stem cells in experimental periodontitis by modulating macrophages through the kynurenine-AhR-NRF2 pathway

doi: 10.1016/j.molmet.2022.101617

Figure Lengend Snippet: Inhibition of IDO activity reduced the therapeutic potential of ASCs in experimental periodontitis and downregulated NRF2 expression. (A) Three-dimensional reconstruction of maxillary alveolar bone in ASC-injected and 1-MT pretreated ASC-injected rats on day 7, day 14, and day 21. Scale bar, 1 mm. (B) Analysis of bone parameters. (C) Immunohistochemical staining of IL-1β, OCN, and NRF2 in the ASC-injected and 1-MT pretreated ASC-injected rats. Scale bar, 50 μm. (D) Representative immunofluorescence staining of M1 phenotype macrophages (iNOS + ; Green) and M2 macrophages (CD206 + ; Red) in sagittal sections of maxillary molars in ASC-injected and 1-MT pretreated ASC-injected rats. Scale bar, 20 μm. (E) The mean IOD of IL-1β, OCN, and NRF2 and the calculated ratio of iNOS + /CD206 + . IOD, integrated optical density.

Article Snippet: For tissues, the sections were incubated with a mouse anti-CD206 monoclonal antibody (1:100, 60143-1-lg, Proteintech), a rabbit anti-NRF2 monoclonal antibody (1:100, A0674, Abclonal), a rabbit anti-inducible nitric oxide synthase (iNOS) polyclonal antibody (1:100, 18985-1-AP, Proteintech) overnight at 4 °C.

Techniques: Inhibition, Activity Assay, Expressing, Injection, Immunohistochemical staining, Staining, Immunofluorescence

Journal: eLife

Article Title: IRF4 haploinsufficiency in a family with Whipple’s disease

doi: 10.7554/eLife.32340

Figure Lengend Snippet: CD11b, CD86, CD206, CD209 and HLA-DR mean fluorescence intensity (MFI) for M2-MDM (left) and M1-MDM (right) from P1 and two healthy unrelated controls ( C1 and C2 ), either left non-stimulated (NS) or stimulated with IL-4 (for M2-like MDMs) or IFN-g (for M1-like MDMs). We showed that CD11b, CD86, CD206, CD209, and HLA-DR expression levels were similar in MDMs from P1 and healthy unrelated controls.

Article Snippet: Antibody , anti-CD206 , Miltenyi Biotec , #130-099-732 , Fluorochrome: PE.

Techniques: Fluorescence, Expressing

Journal: eLife

Article Title: IRF4 haploinsufficiency in a family with Whipple’s disease

doi: 10.7554/eLife.32340

Figure Lengend Snippet:

Article Snippet: Antibody , anti-CD206 , Miltenyi Biotec , #130-099-732 , Fluorochrome: PE.

Techniques: Plasmid Preparation, Isolation, Purification, Transfection, Construct, Generated, Recombinant, Sequencing, TA Cloning, Expressing, Mutagenesis, Staining, Migration, Software

Journal: Journal of Immunology Research

Article Title: Protective Effects of Thalidomide on High-Glucose-Induced Podocyte Injury through In Vitro Modulation of Macrophage M1/M2 Differentiation

doi: 10.1155/2020/8263598

Figure Lengend Snippet: Effects of thalidomide on iNOS, CD206, Arg-1 and TNF- α protein expression in 33.3 mM glucose-induced macrophage. (a) iNOS and CD206 protein expressions. (d) Arg-1 protein expression. (f) TNF- α protein expression. The results of iNOS, CD206, Arg-1, and TNF- α were represented in (b), (c), (e), and (g), respectively. All results were expressed as a ration with respect to control and represented as the mean ± SD in triplicates. ∗ p < 0.05; versus 11.1 mM glucose. # p < 0.05; versus 33.3 mM glucose. & p < 0.05; versus Tha100. ^ p < 0.05; versus Tha50. Abbreviations: LPS: lipopolysaccharide; Tha50: 50 μ g/ml thalidomide in 33.3 mM glucose; Tha100: 100 μ g/ml thalidomide in 33.3 mM glucose; Tha200: 200 μ g/ml thalidomide in 33.3 mM glucose; iNOS: inducible nitric oxide synthase; CD206: mannose receptor; TNF- α : tumor necrosis factor- α ; Arg-1: arginase-1.

Article Snippet: After several consecutive rinses with a washing buffer (0.1% Tween-20 in PBS), the membranes were incubated with primary antibodies against iNOS at 1 : 500 dilution (Catalog No. BA0362, Boster), TNF- α (Catalog No. BA0131, Boster) at 1 : 500 dilution, CD206 (Catalog No. A02285-2, Boster) at 1 : 500 dilution, and antibody against Arg-1 (Catalog No. BM4000, Boster) at 1 : 500 dilution overnight at 4°C.

Techniques: Expressing, Control

Journal: Journal of Immunology Research

Article Title: Protective Effects of Thalidomide on High-Glucose-Induced Podocyte Injury through In Vitro Modulation of Macrophage M1/M2 Differentiation

doi: 10.1155/2020/8263598

Figure Lengend Snippet: Effects of thalidomide on iNOS, CD206, Arg-1, and TNF- α mRNA expressions in 33.3 mM glucose-induced macrophage. (a) iNOS mRNA expression. (b) CD206 mRNA expression. (c) CD206 mRNA expression. (d) TNF- α mRNA expression.All the results were represented as the mean ± SD in triplicates ∗ p < 0.05; versus 11.1 mM glucose. # p < 0.05; versus 33.3 mM glucose. & p < 0.05; versus Tha100. ^ p < 0.05; versus Tha50. Abbreviations: LPS: lipopolysaccharide; Tha50: 50 μ g/ml thalidomide in 33.3 mM glucose; Tha100: 100 μ g/ml thalidomide in 33.3 mM glucose; Tha200: 200 μ g/ml thalidomide in 33.3 mM glucose; iNOS: inducible nitric oxide synthase; CD206: mannose receptor; TNF- α : tumor necrosis factor- α ; Arg-1: arginase-1.

Article Snippet: After several consecutive rinses with a washing buffer (0.1% Tween-20 in PBS), the membranes were incubated with primary antibodies against iNOS at 1 : 500 dilution (Catalog No. BA0362, Boster), TNF- α (Catalog No. BA0131, Boster) at 1 : 500 dilution, CD206 (Catalog No. A02285-2, Boster) at 1 : 500 dilution, and antibody against Arg-1 (Catalog No. BM4000, Boster) at 1 : 500 dilution overnight at 4°C.

Techniques: Expressing