Journal: Scientific Reports

Article Title: Diminution of Phagocytosed Micro/Nanoparticles by Tethering with Immunoregulatory CD200 Protein

doi: 10.1038/s41598-020-65559-z

Figure Lengend Snippet: ( a ) The circular map showing multiple cloning sites for CD200-SA fusion gene. CoreSA was inserted between XhoI and EcoRI; the extracellular domain of human CD200 was inserted between BamHI and EcoRV. ( b ) DNA gel electrophoresis of DNA ladder (lane 1), extracellular domain of human CD200 (~0.6 kb) (lane 2) and coreSA (~0.5 kb) (lane 3).

Article Snippet: Human/mouse CD200 antibody and mouse IgG HRP-conjugated antibody were purchased from R&D Systems (Minneapolis, MN, USA).

Techniques: Cloning, DNA Gel Electrophoresis

Journal: Scientific Reports

Article Title: Diminution of Phagocytosed Micro/Nanoparticles by Tethering with Immunoregulatory CD200 Protein

doi: 10.1038/s41598-020-65559-z

Figure Lengend Snippet: ( a ) Purification of human CD200-SA protein using HisPur Ni-NTA resin. (1) All blue protein standard; (2) crude protein lysate of human CD200-SA using Rosetta competent E. coli cells, induced by 0.4 mM IPTG at 37 °C for 2 h; (3) purified human CD200-SA protein. ( b ) Elution profile of CD200-SA fusion protein purified by Ni-NTA resin. Wash buffer contains 10 mM imidazole and elution buffer contains 250 mM imidazole. ( c ) Western blot of purified protein human CD200-SA with human/mouse CD200 antibody (left) and anti-streptavidin antibody (right) as primary antibody. ( d ) Dot blot analysis of CD200 coated 0.56 µm particles (left), unmodified 0.56 µm particles (middle) and control CD200 protein (right) using human/mouse CD200 antibody as primary antibody. The original SDS-PAGE gel image and Western blot image are provided in the Supplementary inforamtion file.

Article Snippet: Human/mouse CD200 antibody and mouse IgG HRP-conjugated antibody were purchased from R&D Systems (Minneapolis, MN, USA).

Techniques: Purification, Western Blot, Dot Blot, Control, SDS Page

Journal: Scientific Reports

Article Title: Diminution of Phagocytosed Micro/Nanoparticles by Tethering with Immunoregulatory CD200 Protein

doi: 10.1038/s41598-020-65559-z

Figure Lengend Snippet: Size, polydispersity index (PDI) and zeta potential of unmodified and CD200-coated particles.

Article Snippet: Human/mouse CD200 antibody and mouse IgG HRP-conjugated antibody were purchased from R&D Systems (Minneapolis, MN, USA).

Techniques: Zeta Potential Analyzer

Journal: Scientific Reports

Article Title: Diminution of Phagocytosed Micro/Nanoparticles by Tethering with Immunoregulatory CD200 Protein

doi: 10.1038/s41598-020-65559-z

Figure Lengend Snippet: ( a ) The expression level of CD200R on THP-1 macrophages was analyzed by FACS. THP-1 macrophages were treated with anti-human CD200R with PE label. Non-treated macrophages were used as control. The MFI of untreated cells and anti CD200R-PE treated cells were 1,031 ± 83 and 1,266 ± 109, respectively. Data were presented as mean ± standard deviation (n = 3). ( b ) FACS analysis of THP-1 macrophages engulfing control and CD200 coated 0.56 µm polystyrene particles for 2 h. FACS analysis showed that CD200 coated particles decreased TLR4 expression on THP-1 macrophages. The MFI of untreated cells and anti TLR4-FITC treated cells were 8,409 ± 917 and 3,965 ± 324, respectively. Data were presented as mean ± standard deviation (n = 3).

Article Snippet: Human/mouse CD200 antibody and mouse IgG HRP-conjugated antibody were purchased from R&D Systems (Minneapolis, MN, USA).

Techniques: Expressing, Control, Standard Deviation

Journal: Scientific Reports

Article Title: Diminution of Phagocytosed Micro/Nanoparticles by Tethering with Immunoregulatory CD200 Protein

doi: 10.1038/s41598-020-65559-z

Figure Lengend Snippet: ( a ) Bright field, fluorescence and overlay images of THP-1 macrophages engulfing (i) unmodified fluorescent polystyrene particles, (ii) CD200-coated fluorescent polystyrene particles, and (iii) CD200-coated fluorescent polystyrene particles blocked with anti-CD200 antibody. Cells were incubated with 0.15, 0.56, 0.84 µm and 2 µm polystyrene particles for 5 h. Scale bar denotes 100 μm. ( b ) Relative fluorescence intensity of THP-1 macrophages engulfing unmodified fluorescent particles, CD200-coated fluorescent particles, and CD200-coated fluorescent particles blocked with anti-CD200 antibody at 2, 5 and 10 h post-treatment. Data were presented as mean ± standard deviation (n = 3). *Denotes p < 0.05.

Article Snippet: Human/mouse CD200 antibody and mouse IgG HRP-conjugated antibody were purchased from R&D Systems (Minneapolis, MN, USA).

Techniques: Fluorescence, Incubation, Standard Deviation

Journal: Scientific Reports

Article Title: Diminution of Phagocytosed Micro/Nanoparticles by Tethering with Immunoregulatory CD200 Protein

doi: 10.1038/s41598-020-65559-z

Figure Lengend Snippet: Bright field, fluorescence and overlay images of THP-1 macrophages engulfing (i) unmodified and (ii) CD200-coated fluorescent polystyrene particles (0.56 and 2 µm) at 2, 5 and 10 h post-treatment. Scale bar denotes 100 μm.

Article Snippet: Human/mouse CD200 antibody and mouse IgG HRP-conjugated antibody were purchased from R&D Systems (Minneapolis, MN, USA).

Techniques: Fluorescence

Journal: Scientific Reports

Article Title: Diminution of Phagocytosed Micro/Nanoparticles by Tethering with Immunoregulatory CD200 Protein

doi: 10.1038/s41598-020-65559-z

Figure Lengend Snippet: THP-1 macrophages were allowed to engulf ( a ) unmodified ( b ) SA coated ( c ) CD200-SA coated 0.56 µm polystyrene particles for 5 h. Scale bar denotes 100 µm. ( d ) Relative fluorescence intensity of THP-1 macrophages engulfing unmodified, SA-coated and CD200-coated 0.56 µm polystyrene particles at 2, 5 and 10 h post-treatment. Data were presented as mean ± standard deviation (n = 3). *Denotes p < 0.05.

Article Snippet: Human/mouse CD200 antibody and mouse IgG HRP-conjugated antibody were purchased from R&D Systems (Minneapolis, MN, USA).

Techniques: Fluorescence, Standard Deviation

Journal: Scientific Reports

Article Title: Diminution of Phagocytosed Micro/Nanoparticles by Tethering with Immunoregulatory CD200 Protein

doi: 10.1038/s41598-020-65559-z

Figure Lengend Snippet: Bright field, fluorescence and overlay image of THP-1 macrophages engulfing ( a ) FITC-zymosan particles and ( b ) CD200-coated FITC-zymosan particles for 5 h. Scale bar denotes 100 μm. ( c ) Relative fluorescence intensity of THP-1 macrophages engulfing unmodified and CD200-coated FITC-zymosan particles at 2, 5 and 10 h post-treatment. Data were presented as mean ± standard deviation (n = 3). *Denotes p < 0.05.

Article Snippet: Human/mouse CD200 antibody and mouse IgG HRP-conjugated antibody were purchased from R&D Systems (Minneapolis, MN, USA).

Techniques: Fluorescence, Standard Deviation

Journal: Scientific Reports

Article Title: Diminution of Phagocytosed Micro/Nanoparticles by Tethering with Immunoregulatory CD200 Protein

doi: 10.1038/s41598-020-65559-z

Figure Lengend Snippet: THP-1 macrophages were allowed to engulf unmodified and CD200 coated 0.56 µm polystyrene particles for 18 h. After 18 h, culture media were collected and concentrations of ( a ) IL-6 and ( b ) TNF-α were measured by commercial ELISA kits. Data were presented as mean ± standard deviation (n = 3). *Denotes p = 0.006.

Article Snippet: Human/mouse CD200 antibody and mouse IgG HRP-conjugated antibody were purchased from R&D Systems (Minneapolis, MN, USA).

Techniques: Enzyme-linked Immunosorbent Assay, Standard Deviation

Journal: ERJ Open Research

Article Title: Distinct immune regulatory receptor profiles linked to altered monocyte subsets in sarcoidosis

doi: 10.1183/23120541.00804-2020

Figure Lengend Snippet: Distribution of the regulatory receptor CD200R and its ligand CD200L in sarcoidosis granulomas: transbronchial lung biopsy samples from two patients with sarcoidosis stained for a) CD200L and b) CD200R, with respective isotype control antibody staining in c) and d). f: fibroblasts; h: histiocytes (macrophages). Original magnification ×100.

Article Snippet: Antibodies were a polyclonal goat CD200L antibody (AF2724; R&D Systems, Abingdon, UK), normal goat IgG control (AB-108-C; R&D Systems), mouse monoclonal IgG1 anti-human CD200R (OX108, MCA 2282T; AbD Serotec/Bio-Rad, Kidlington, UK) and mouse monoclonal control IgG1 antibody (MOPC-21, 400101; Biolegend, London, UK).

Techniques: Staining, Control

Journal: Stem cells (Dayton, Ohio)

Article Title: CD200 Expression Marks a Population of Quiescent Limbal Epithelial Stem Cells with Holoclone Forming Ability.

doi: 10.1002/stem.2903

Figure Lengend Snippet: Figure 2. CD200 expression in human and mouse cornea in vivo and during ex vivo expansion of human limbal epithelial cells. (A): Quantification of CD200 expression through different passages of limbal epithelial cells by flow cytometry. Values represent mean SEM, n = 3–10 (n, number of biological replicates), *, p < .05. (B): Quantification of CD200 expression during calcium induced differentiation of limbal epithelial cells by flow cytometry. Values represent mean SEM, n = 3, *, p < .05. (C): Immunohistochemical staining of human corneal tissue paraffin sections for ΔNp63 and CD200 within the central cornea and limbus. Nuclei are shown by Hoechst counter stain- ing. Scale bars 20 μm. (D): Immunohistochemical staining of murine corneal tissue cryosections for CK15, ΔNp63, and CD200 within the central cornea and limbus. Nuclei are shown by DAPI counter staining. The dashed line indicates the stromal-epithelial junction. Red arrows point at limbal region. Scale bars 20 μm. (E): Immunohistochemical staining of limbal epithelial cell colonies in vitro for CD200 and ΔNp63. Blue arrow points CD200+ cells. Nuclei are shown by Hoechst counter staining. Scale bar 50 μm. (F): Immunohistochemical stain- ing of limbal epithelial cell colonies in vitro for CD200 and Ki67. Red arrows point to CD200+ Ki67+ cells; orange arrows point to CD200+Ki67−cells. Nuclei are shown by Hoechst counter staining. Scale bar 50 μm. Abbreviations: ep, epithelium; st, stroma.

Article Snippet: The following primary antibodies were used at the indicated dilutions: anti CD109 (sc-271085, Santa Cruz, USA, 1:200), anti-human CD200 (329201, BioLegend, USA, 1:200), anti-mouse CD200 (AF3355, Novus Biologicals, USA, 1:100), anti p63 delta (NBP2-29467, Novus Biologicals, USA, 1:200), anti-cytokeratin 15 (ab52816, Abcam, Cambridge, UK, 1:100), and anti Ki67 antibody (ab15580, Abcam, UK, 1:100).

Techniques: Expressing, In Vivo, Ex Vivo, Cytometry, Immunohistochemical staining, Staining, In Vitro

Journal: Stem cells (Dayton, Ohio)

Article Title: CD200 Expression Marks a Population of Quiescent Limbal Epithelial Stem Cells with Holoclone Forming Ability.

doi: 10.1002/stem.2903

Figure Lengend Snippet: Figure 3. Colony forming efficiency and proliferative potential of sorted CD200 positive and negative population. (A): Pie chart showing the distribution of formed and aborted colonies in CD200+ population. (B): Comparison of colony forming efficiencies of CD200+ and CD200−cells. Values represent mean SEM, n = 3 (n, number of biological replicates). (C): Pie chart showing the distribution of formed and aborted colonies in CD200−population. Values represent mean SEM, n = 3. (D): Microscopic and macroscopic appearances of colo- nies formed by CD200+ cells. Scale bars 100 μm. (E): Microscopic and macroscopic appearances of colonies formed by CD200−cells. Scale bars 100 μm. (F): BrdU cell proliferation assay of CD200 negative and positive limbal epithelial cell population after 1- and 8-hours incuba- tion with BrdU. Values represent mean SEM, n = 3.(G): Quantification of cells in the S phase of the cell cycle in CD200+ and CD200−

Article Snippet: The following primary antibodies were used at the indicated dilutions: anti CD109 (sc-271085, Santa Cruz, USA, 1:200), anti-human CD200 (329201, BioLegend, USA, 1:200), anti-mouse CD200 (AF3355, Novus Biologicals, USA, 1:100), anti p63 delta (NBP2-29467, Novus Biologicals, USA, 1:200), anti-cytokeratin 15 (ab52816, Abcam, Cambridge, UK, 1:100), and anti Ki67 antibody (ab15580, Abcam, UK, 1:100).

Techniques: Comparison, BrdU Cell Proliferation Assay

Journal: Stem cells (Dayton, Ohio)

Article Title: CD200 Expression Marks a Population of Quiescent Limbal Epithelial Stem Cells with Holoclone Forming Ability.

doi: 10.1002/stem.2903

Figure Lengend Snippet: Figure 5. CD200 knockdown and its effect on clonal ability of limbal epithelial cells. (A): Quantitative reverse transcriptase poly- merase chain reaction expression data for control siRNA versus CD200 siRNA treated limbal epithelial cells. Values represent mean SEM, n = 3 (n, number of biological replicates), *, p < .05. (B): Pie chart showing distribution of paraclones, meroclones, and holoclones formed by control siRNA treated cells and (C) CD200 siRNA treated cells. (D): Representative images of colonies formed in control and CD200 siRNA group, with 500 or 1,000 cells seeded per well. Abbreviation: siRNA, small interfering RNA.

Article Snippet: The following primary antibodies were used at the indicated dilutions: anti CD109 (sc-271085, Santa Cruz, USA, 1:200), anti-human CD200 (329201, BioLegend, USA, 1:200), anti-mouse CD200 (AF3355, Novus Biologicals, USA, 1:100), anti p63 delta (NBP2-29467, Novus Biologicals, USA, 1:200), anti-cytokeratin 15 (ab52816, Abcam, Cambridge, UK, 1:100), and anti Ki67 antibody (ab15580, Abcam, UK, 1:100).

Techniques: Knockdown, Reverse Transcription, Expressing, Control, Small Interfering RNA

Journal: Stem cells (Dayton, Ohio)

Article Title: CD200 Expression Marks a Population of Quiescent Limbal Epithelial Stem Cells with Holoclone Forming Ability.

doi: 10.1002/stem.2903

Figure Lengend Snippet: Figure 4. Expression of putative limbal stem cell and corneal epi- thelial cell markers in the sorted CD109 and CD200 positive and negative cell populations. (A): Quantitative reverse transcriptase polymerase chain reaction expression data for CD109+ limbal epi- thelial cell population versus CD109−limbal epithelial cell popula- tion represented by the red line (value 1). Values represent mean SEM, n = 3 (n, number of biological replicates), *, p < .05; **, p < .01; ***, p < .001. (B): Quantitative reverse transcriptase polymerase chain reaction expression data for CD200+ limbal epi- thelial cell population versus CD200−limbal epithelial cell popula- tion represented by the red line (value 1). Values represent mean SEM, n = 3, *, p < .05; **, p < .01; ***, p < .001.

Article Snippet: The following primary antibodies were used at the indicated dilutions: anti CD109 (sc-271085, Santa Cruz, USA, 1:200), anti-human CD200 (329201, BioLegend, USA, 1:200), anti-mouse CD200 (AF3355, Novus Biologicals, USA, 1:100), anti p63 delta (NBP2-29467, Novus Biologicals, USA, 1:200), anti-cytokeratin 15 (ab52816, Abcam, Cambridge, UK, 1:100), and anti Ki67 antibody (ab15580, Abcam, UK, 1:100).

Techniques: Expressing, Reverse Transcription, Polymerase Chain Reaction

Journal: Frontiers in Cell and Developmental Biology

Article Title: Electroacupuncture Reduces Oocyte Number and Maintains Vascular Barrier Against Ovarian Hyperstimulation Syndrome by Regulating CD200

doi: 10.3389/fcell.2021.648578

Figure Lengend Snippet: The level of CD200 in follicular fluid and serum of patients who underwent IVF procedures. Correlations between CD200 concentration and the number of oocytes retrieved in FF (A) and serum (C) ( n = 43). The levels of CD200 in follicular fluid (B) and serum (D) were compared between the groups with higher (≥18, n = 12), middle (6–17, n = 23), and lower (≤5, n = 8) oocytes retrieved. The correlations between CD200 concentration and the level of E2 and P were analyzed (E) . ∗∗∗ P < 0.001. Abbreviations: E2, estradiol; P, progesterone.

Article Snippet: Recombinant Human CD200 Fc Chimera Protein was purchased from R&D Systems (#2724-CD).

Techniques: Concentration Assay

Journal: Frontiers in Cell and Developmental Biology

Article Title: Electroacupuncture Reduces Oocyte Number and Maintains Vascular Barrier Against Ovarian Hyperstimulation Syndrome by Regulating CD200

doi: 10.3389/fcell.2021.648578

Figure Lengend Snippet: Effects of hCG on CD200 and inflammatory response pathway in KGN cells and the effects of CM on vascular permeability in HUVECs. The cells were treated with vehicle control (Ctrl) and 1, 10, and 100 IU/mL hCG for 24 h. The protein levels of CD200 and CD200R (A) , Cyp19a1, IL-1β, and TNFα (B) , and of NF-κB, p-NF-κB, p38 MAPK, and p-p38 MAPK (C) were examined. HUVECs were treated with condition culture medium from KGN for 24 h. (D) The levels of vasoactive proteins VEGF and VEGFR2, and cell junction proteins of Occludin, Claudin 5, and ZO-1 in HUVECs. (E) Fluorescent photomicrographs of HUVEC monolayer stained with TRITC-phalloidin with a final concentration of 20 mg/ml. Data were presented as means ± SD, * P < 0.5, ** P < 0.01, *** P < 0.001 versus Ctrl.

Article Snippet: Recombinant Human CD200 Fc Chimera Protein was purchased from R&D Systems (#2724-CD).

Techniques: Permeability, Control, Staining, Concentration Assay

Journal: Frontiers in Cell and Developmental Biology

Article Title: Electroacupuncture Reduces Oocyte Number and Maintains Vascular Barrier Against Ovarian Hyperstimulation Syndrome by Regulating CD200

doi: 10.3389/fcell.2021.648578

Figure Lengend Snippet: Effects of CD200Fc on inflammatory response pathway in KGN cells and vascular permeability-related protein and cell cytoskeleton in HUVECs. (A) The cells were treated with vehicle (Ctrl), and 10 IU/ml hCG with or without 100 ng/ml CD200Fc for 24 h, and the protein levels of CD200, CD200R, Cyp19a1, IL-1β, and TNFα were examined by western blotting. (B) The ovarian expression of NF-κB, p-NF-κB, p38 MAPK, and p-p38 MAPK, and the relative NF-κB and p38 MAPK activity were derived as p-NF-κB and p-p38 MAPK, and are expressed as fold changes over Ctrl. HUVECs were treated with condition culture medium from KGN (10 IU/ml hCG with or without 100 ng/ml CD200Fc) for 24 h. (C) The levels of vasoactive proteins VEGF and VEGFR2, and cell junction proteins Occludin, Claudin 5, and ZO-1 were examined by western blotting. (D) Fluorescent photomicrographs of HUVECs monolayer stained with TRITC-phalloidin with a final concentration of 20 mg/ml. Data were presented as means ± SD, * P < 0.5, ** P < 0.01, *** P < 0.001 versus Ctrl.

Article Snippet: Recombinant Human CD200 Fc Chimera Protein was purchased from R&D Systems (#2724-CD).

Techniques: Permeability, Western Blot, Expressing, Activity Assay, Derivative Assay, Staining, Concentration Assay

Journal: Frontiers in Cell and Developmental Biology

Article Title: Electroacupuncture Reduces Oocyte Number and Maintains Vascular Barrier Against Ovarian Hyperstimulation Syndrome by Regulating CD200

doi: 10.3389/fcell.2021.648578

Figure Lengend Snippet: The number of follicles at different stages, expression of CD200 and CD200R in EA-treated OHSS model. (A) The number of follicles at different stages in each group. (B) The ratio of corpora lutea/total follicles. (C) The protein expression of CD200 and CD200R in ovary of OHSS rats with or without EA treatment. (D) The CD200 levels in the serum of experimental groups were detected by ELISA. (E) The distribution of CD200 was detected by IHC, original magnification 40×, scale bar = 100 μm. Data were shown as means ± SD, * P < 0.5, ** P < 0.01, *** P < 0.001 versus OHSS group.

Article Snippet: Recombinant Human CD200 Fc Chimera Protein was purchased from R&D Systems (#2724-CD).

Techniques: Expressing, Enzyme-linked Immunosorbent Assay

Journal: Cell reports

Article Title: Single-cell RNA sequencing identifies a population of human liver-type ILC1s

doi: 10.1016/j.celrep.2022.111937

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: CD200R Biotin (REA725) , Miltenyi , RRID: AB_2656117; Cat#130-111-289.

Techniques: Blocking Assay, Recombinant, Clinical Proteomics, Nucleic Acid Electrophoresis, Software, Inverted Microscopy

Journal: International Journal of Hematology-Oncology and Stem Cell Research

Article Title: Evaluation of CD160 and CD200 Expression as Differentiating Markers between Chronic Lymphocytic Leukemia and Other Mature B-Cell Neoplasms

doi:

Figure Lengend Snippet: Expression of CD160 and CD200 on B lymphocytes of healthy subjects and on leukemic cell clone of the patients

Article Snippet: In addition Human CD160 APC-conjugated Antibody, clone 688327 and Human CD200 percp-conjugated Antibody, clone 325516 from R&D systems- Biotechne, USA were added to our panel and analyzed for both patients and controls.

Techniques: Expressing, Control

Journal: International Journal of Hematology-Oncology and Stem Cell Research

Article Title: Evaluation of CD160 and CD200 Expression as Differentiating Markers between Chronic Lymphocytic Leukemia and Other Mature B-Cell Neoplasms

doi:

Figure Lengend Snippet: CD200 expression on different B-cell neoplasms and the control group

Article Snippet: In addition Human CD160 APC-conjugated Antibody, clone 688327 and Human CD200 percp-conjugated Antibody, clone 325516 from R&D systems- Biotechne, USA were added to our panel and analyzed for both patients and controls.

Techniques: Expressing, Control

Journal: International Journal of Hematology-Oncology and Stem Cell Research

Article Title: Evaluation of CD160 and CD200 Expression as Differentiating Markers between Chronic Lymphocytic Leukemia and Other Mature B-Cell Neoplasms

doi:

Figure Lengend Snippet: ROC curves of CD160 and CD200 for diagnosing CLL from the other MBN

Article Snippet: In addition Human CD160 APC-conjugated Antibody, clone 688327 and Human CD200 percp-conjugated Antibody, clone 325516 from R&D systems- Biotechne, USA were added to our panel and analyzed for both patients and controls.

Techniques:

Journal: International Journal of Hematology-Oncology and Stem Cell Research

Article Title: Evaluation of CD160 and CD200 Expression as Differentiating Markers between Chronic Lymphocytic Leukemia and Other Mature B-Cell Neoplasms

doi:

Figure Lengend Snippet: ROC curves for combined expression of CD160 & CD200 to diagnose CLL cases from the MBN.

Article Snippet: In addition Human CD160 APC-conjugated Antibody, clone 688327 and Human CD200 percp-conjugated Antibody, clone 325516 from R&D systems- Biotechne, USA were added to our panel and analyzed for both patients and controls.

Techniques: Expressing

Journal: International Journal of Hematology-Oncology and Stem Cell Research

Article Title: Evaluation of CD160 and CD200 Expression as Differentiating Markers between Chronic Lymphocytic Leukemia and Other Mature B-Cell Neoplasms

doi:

Figure Lengend Snippet: Distribution of patients (CLL and other MBN) and control according to CD 160 and CD200 percentage expression.

Article Snippet: In addition Human CD160 APC-conjugated Antibody, clone 688327 and Human CD200 percp-conjugated Antibody, clone 325516 from R&D systems- Biotechne, USA were added to our panel and analyzed for both patients and controls.

Techniques: Control, Expressing

Journal: International Journal of Hematology-Oncology and Stem Cell Research

Article Title: Evaluation of CD160 and CD200 Expression as Differentiating Markers between Chronic Lymphocytic Leukemia and Other Mature B-Cell Neoplasms

doi:

Figure Lengend Snippet: Distribution of patients (CLL and other MBN) and control according to CD 160 and CD200 MFIR.

Article Snippet: In addition Human CD160 APC-conjugated Antibody, clone 688327 and Human CD200 percp-conjugated Antibody, clone 325516 from R&D systems- Biotechne, USA were added to our panel and analyzed for both patients and controls.

Techniques: Control