Journal: PLoS ONE

Article Title: CD200R/CD200 Inhibits Osteoclastogenesis: New Mechanism of Osteoclast Control by Mesenchymal Stem Cells in Human

doi: 10.1371/journal.pone.0072831

Figure Lengend Snippet: PBMC adherent cells were co-cultured with MSCs in presence of M-CSF for 48 hr. RANKL was added after 48 hr and half of the medium was changed every 3 days. (A) After 21 days of culture, actin rings were stained with phalloidin-Alexa 540 and nuclei with DAPI. (B) Osteoclast precursors were cultivated with MSCs, CD200 + MSCs or CD200 – MSCs for 21 days. Tartrate-resistant acid phosphatase (TRAP) assay was performed and area of TRAP-positive cells was measured. Data are mean±SD. *, P<0.05. (C) Effect of MSCs on bone resorption activity of osteoclasts. White arrows represent bone resorption pits. The results of 1 experiment out of 3 are presented.

Article Snippet: Recombinant human CD200 Fc chimera protein (rCD200) was from R&D Systems (Minneapolis, MN, USA).

Techniques: Cell Culture, Staining, TRAP Assay, Activity Assay

Journal: Stem cells (Dayton, Ohio)

Article Title: CD200 Expression Marks a Population of Quiescent Limbal Epithelial Stem Cells with Holoclone Forming Ability.

doi: 10.1002/stem.2903

Figure Lengend Snippet: Figure 2. CD200 expression in human and mouse cornea in vivo and during ex vivo expansion of human limbal epithelial cells. (A): Quantification of CD200 expression through different passages of limbal epithelial cells by flow cytometry. Values represent mean SEM, n = 3–10 (n, number of biological replicates), *, p < .05. (B): Quantification of CD200 expression during calcium induced differentiation of limbal epithelial cells by flow cytometry. Values represent mean SEM, n = 3, *, p < .05. (C): Immunohistochemical staining of human corneal tissue paraffin sections for ΔNp63 and CD200 within the central cornea and limbus. Nuclei are shown by Hoechst counter stain- ing. Scale bars 20 μm. (D): Immunohistochemical staining of murine corneal tissue cryosections for CK15, ΔNp63, and CD200 within the central cornea and limbus. Nuclei are shown by DAPI counter staining. The dashed line indicates the stromal-epithelial junction. Red arrows point at limbal region. Scale bars 20 μm. (E): Immunohistochemical staining of limbal epithelial cell colonies in vitro for CD200 and ΔNp63. Blue arrow points CD200+ cells. Nuclei are shown by Hoechst counter staining. Scale bar 50 μm. (F): Immunohistochemical stain- ing of limbal epithelial cell colonies in vitro for CD200 and Ki67. Red arrows point to CD200+ Ki67+ cells; orange arrows point to CD200+Ki67−cells. Nuclei are shown by Hoechst counter staining. Scale bar 50 μm. Abbreviations: ep, epithelium; st, stroma.

Article Snippet: The following primary antibodies were used at the indicated dilutions: anti CD109 (sc-271085, Santa Cruz, USA, 1:200), anti-human CD200 (329201, BioLegend, USA, 1:200), anti-mouse CD200 (AF3355, Novus Biologicals, USA, 1:100), anti p63 delta (NBP2-29467, Novus Biologicals, USA, 1:200), anti-cytokeratin 15 (ab52816, Abcam, Cambridge, UK, 1:100), and anti Ki67 antibody (ab15580, Abcam, UK, 1:100).

Techniques: Expressing, In Vivo, Ex Vivo, Cytometry, Immunohistochemical staining, Staining, In Vitro

Journal: Stem cells (Dayton, Ohio)

Article Title: CD200 Expression Marks a Population of Quiescent Limbal Epithelial Stem Cells with Holoclone Forming Ability.

doi: 10.1002/stem.2903

Figure Lengend Snippet: Figure 3. Colony forming efficiency and proliferative potential of sorted CD200 positive and negative population. (A): Pie chart showing the distribution of formed and aborted colonies in CD200+ population. (B): Comparison of colony forming efficiencies of CD200+ and CD200−cells. Values represent mean SEM, n = 3 (n, number of biological replicates). (C): Pie chart showing the distribution of formed and aborted colonies in CD200−population. Values represent mean SEM, n = 3. (D): Microscopic and macroscopic appearances of colo- nies formed by CD200+ cells. Scale bars 100 μm. (E): Microscopic and macroscopic appearances of colonies formed by CD200−cells. Scale bars 100 μm. (F): BrdU cell proliferation assay of CD200 negative and positive limbal epithelial cell population after 1- and 8-hours incuba- tion with BrdU. Values represent mean SEM, n = 3.(G): Quantification of cells in the S phase of the cell cycle in CD200+ and CD200−

Article Snippet: The following primary antibodies were used at the indicated dilutions: anti CD109 (sc-271085, Santa Cruz, USA, 1:200), anti-human CD200 (329201, BioLegend, USA, 1:200), anti-mouse CD200 (AF3355, Novus Biologicals, USA, 1:100), anti p63 delta (NBP2-29467, Novus Biologicals, USA, 1:200), anti-cytokeratin 15 (ab52816, Abcam, Cambridge, UK, 1:100), and anti Ki67 antibody (ab15580, Abcam, UK, 1:100).

Techniques: Comparison, BrdU Cell Proliferation Assay

Journal: Stem cells (Dayton, Ohio)

Article Title: CD200 Expression Marks a Population of Quiescent Limbal Epithelial Stem Cells with Holoclone Forming Ability.

doi: 10.1002/stem.2903

Figure Lengend Snippet: Figure 5. CD200 knockdown and its effect on clonal ability of limbal epithelial cells. (A): Quantitative reverse transcriptase poly- merase chain reaction expression data for control siRNA versus CD200 siRNA treated limbal epithelial cells. Values represent mean SEM, n = 3 (n, number of biological replicates), *, p < .05. (B): Pie chart showing distribution of paraclones, meroclones, and holoclones formed by control siRNA treated cells and (C) CD200 siRNA treated cells. (D): Representative images of colonies formed in control and CD200 siRNA group, with 500 or 1,000 cells seeded per well. Abbreviation: siRNA, small interfering RNA.

Article Snippet: The following primary antibodies were used at the indicated dilutions: anti CD109 (sc-271085, Santa Cruz, USA, 1:200), anti-human CD200 (329201, BioLegend, USA, 1:200), anti-mouse CD200 (AF3355, Novus Biologicals, USA, 1:100), anti p63 delta (NBP2-29467, Novus Biologicals, USA, 1:200), anti-cytokeratin 15 (ab52816, Abcam, Cambridge, UK, 1:100), and anti Ki67 antibody (ab15580, Abcam, UK, 1:100).

Techniques: Knockdown, Reverse Transcription, Expressing, Control, Small Interfering RNA

Journal: Stem cells (Dayton, Ohio)

Article Title: CD200 Expression Marks a Population of Quiescent Limbal Epithelial Stem Cells with Holoclone Forming Ability.

doi: 10.1002/stem.2903

Figure Lengend Snippet: Figure 4. Expression of putative limbal stem cell and corneal epi- thelial cell markers in the sorted CD109 and CD200 positive and negative cell populations. (A): Quantitative reverse transcriptase polymerase chain reaction expression data for CD109+ limbal epi- thelial cell population versus CD109−limbal epithelial cell popula- tion represented by the red line (value 1). Values represent mean SEM, n = 3 (n, number of biological replicates), *, p < .05; **, p < .01; ***, p < .001. (B): Quantitative reverse transcriptase polymerase chain reaction expression data for CD200+ limbal epi- thelial cell population versus CD200−limbal epithelial cell popula- tion represented by the red line (value 1). Values represent mean SEM, n = 3, *, p < .05; **, p < .01; ***, p < .001.

Article Snippet: The following primary antibodies were used at the indicated dilutions: anti CD109 (sc-271085, Santa Cruz, USA, 1:200), anti-human CD200 (329201, BioLegend, USA, 1:200), anti-mouse CD200 (AF3355, Novus Biologicals, USA, 1:100), anti p63 delta (NBP2-29467, Novus Biologicals, USA, 1:200), anti-cytokeratin 15 (ab52816, Abcam, Cambridge, UK, 1:100), and anti Ki67 antibody (ab15580, Abcam, UK, 1:100).

Techniques: Expressing, Reverse Transcription, Polymerase Chain Reaction

Journal: ERJ Open Research

Article Title: Distinct immune regulatory receptor profiles linked to altered monocyte subsets in sarcoidosis

doi: 10.1183/23120541.00804-2020

Figure Lengend Snippet: Distribution of the regulatory receptor CD200R and its ligand CD200L in sarcoidosis granulomas: transbronchial lung biopsy samples from two patients with sarcoidosis stained for a) CD200L and b) CD200R, with respective isotype control antibody staining in c) and d). f: fibroblasts; h: histiocytes (macrophages). Original magnification ×100.

Article Snippet: Antibodies were a polyclonal goat CD200L antibody (AF2724; R&D Systems, Abingdon, UK), normal goat IgG control (AB-108-C; R&D Systems), mouse monoclonal IgG1 anti-human CD200R (OX108, MCA 2282T; AbD Serotec/Bio-Rad, Kidlington, UK) and mouse monoclonal control IgG1 antibody (MOPC-21, 400101; Biolegend, London, UK).

Techniques: Staining, Control

Journal: International Journal of Hematology-Oncology and Stem Cell Research

Article Title: Evaluation of CD160 and CD200 Expression as Differentiating Markers between Chronic Lymphocytic Leukemia and Other Mature B-Cell Neoplasms

doi:

Figure Lengend Snippet: Expression of CD160 and CD200 on B lymphocytes of healthy subjects and on leukemic cell clone of the patients

Article Snippet: In addition Human CD160 APC-conjugated Antibody, clone 688327 and Human CD200 percp-conjugated Antibody, clone 325516 from R&D systems- Biotechne, USA were added to our panel and analyzed for both patients and controls.

Techniques: Expressing, Control

Journal: International Journal of Hematology-Oncology and Stem Cell Research

Article Title: Evaluation of CD160 and CD200 Expression as Differentiating Markers between Chronic Lymphocytic Leukemia and Other Mature B-Cell Neoplasms

doi:

Figure Lengend Snippet: CD200 expression on different B-cell neoplasms and the control group

Article Snippet: In addition Human CD160 APC-conjugated Antibody, clone 688327 and Human CD200 percp-conjugated Antibody, clone 325516 from R&D systems- Biotechne, USA were added to our panel and analyzed for both patients and controls.

Techniques: Expressing, Control

Journal: International Journal of Hematology-Oncology and Stem Cell Research

Article Title: Evaluation of CD160 and CD200 Expression as Differentiating Markers between Chronic Lymphocytic Leukemia and Other Mature B-Cell Neoplasms

doi:

Figure Lengend Snippet: ROC curves of CD160 and CD200 for diagnosing CLL from the other MBN

Article Snippet: In addition Human CD160 APC-conjugated Antibody, clone 688327 and Human CD200 percp-conjugated Antibody, clone 325516 from R&D systems- Biotechne, USA were added to our panel and analyzed for both patients and controls.

Techniques:

Journal: International Journal of Hematology-Oncology and Stem Cell Research

Article Title: Evaluation of CD160 and CD200 Expression as Differentiating Markers between Chronic Lymphocytic Leukemia and Other Mature B-Cell Neoplasms

doi:

Figure Lengend Snippet: ROC curves for combined expression of CD160 & CD200 to diagnose CLL cases from the MBN.

Article Snippet: In addition Human CD160 APC-conjugated Antibody, clone 688327 and Human CD200 percp-conjugated Antibody, clone 325516 from R&D systems- Biotechne, USA were added to our panel and analyzed for both patients and controls.

Techniques: Expressing

Journal: International Journal of Hematology-Oncology and Stem Cell Research

Article Title: Evaluation of CD160 and CD200 Expression as Differentiating Markers between Chronic Lymphocytic Leukemia and Other Mature B-Cell Neoplasms

doi:

Figure Lengend Snippet: Distribution of patients (CLL and other MBN) and control according to CD 160 and CD200 percentage expression.

Article Snippet: In addition Human CD160 APC-conjugated Antibody, clone 688327 and Human CD200 percp-conjugated Antibody, clone 325516 from R&D systems- Biotechne, USA were added to our panel and analyzed for both patients and controls.

Techniques: Control, Expressing

Journal: International Journal of Hematology-Oncology and Stem Cell Research

Article Title: Evaluation of CD160 and CD200 Expression as Differentiating Markers between Chronic Lymphocytic Leukemia and Other Mature B-Cell Neoplasms

doi:

Figure Lengend Snippet: Distribution of patients (CLL and other MBN) and control according to CD 160 and CD200 MFIR.

Article Snippet: In addition Human CD160 APC-conjugated Antibody, clone 688327 and Human CD200 percp-conjugated Antibody, clone 325516 from R&D systems- Biotechne, USA were added to our panel and analyzed for both patients and controls.

Techniques: Control