Journal: Arthritis Research & Therapy

Article Title: Characterization of inflammatory cell infiltrate of scleroderma skin: B cells and skin score progression

doi: 10.1186/s13075-018-1569-0

Figure Lengend Snippet: CD68 + , CD3 + , CD20 + and CD138 + cell counts on paired skin specimens in the 28 patients with SSc

Article Snippet: Sections were incubated with anti-CD3 mouse monoclonal antibodies (mAb) (1:50 dilution; Clone PS1, Abcam, Cambridge, MA, USA) or anti-CD20 mouse mAb (1:100 dilution; Clone L26; Novus Biologicals, Littleton, CO, USA) or human anti-CD138 (Syndecan-1) rabbit polyclonal antibodies (Ab) (1:25 dilution; Spring Bioscience, CA, USA).

Techniques:

Journal: Arthritis Research & Therapy

Article Title: Characterization of inflammatory cell infiltrate of scleroderma skin: B cells and skin score progression

doi: 10.1186/s13075-018-1569-0

Figure Lengend Snippet: CD20 cell staining in involved and uninvolved skin from a patient with systemic sclerosis (SSc). a Cellular infiltrate in involved skin (distal forearm biopsy) from a patient with early anti-Scl-70 + diffuse SSc showing an appreciable number of CD20 + cells in perivascular areas. b Uninvolved skin (buttock biopsy) from the same patient showing the presence of a smaller number of CD20 + cells around blood vessels. Original magnification × 400 ( a and b ). Nuclei were counterstained by hematoxylin. Bottom insets show the infiltrating cells at a lower magnification (× 200). Insets at the top of the figures show hematoxylin and eosin (H&E) staining of involved and uninvolved skin (× 50)

Article Snippet: Sections were incubated with anti-CD3 mouse monoclonal antibodies (mAb) (1:50 dilution; Clone PS1, Abcam, Cambridge, MA, USA) or anti-CD20 mouse mAb (1:100 dilution; Clone L26; Novus Biologicals, Littleton, CO, USA) or human anti-CD138 (Syndecan-1) rabbit polyclonal antibodies (Ab) (1:25 dilution; Spring Bioscience, CA, USA).

Techniques: Staining

Journal: Arthritis Research & Therapy

Article Title: Characterization of inflammatory cell infiltrate of scleroderma skin: B cells and skin score progression

doi: 10.1186/s13075-018-1569-0

Figure Lengend Snippet: Clinical and histological characteristics according to immunosuppressive/immunomodulatory treatment and clinical outcome in the 28 patients with SSc

Article Snippet: Sections were incubated with anti-CD3 mouse monoclonal antibodies (mAb) (1:50 dilution; Clone PS1, Abcam, Cambridge, MA, USA) or anti-CD20 mouse mAb (1:100 dilution; Clone L26; Novus Biologicals, Littleton, CO, USA) or human anti-CD138 (Syndecan-1) rabbit polyclonal antibodies (Ab) (1:25 dilution; Spring Bioscience, CA, USA).

Techniques:

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: LRRK2 Is Involved in the IFN-? Response and Host Response to Pathogens

doi: 10.4049/jimmunol.1000548

Figure Lengend Snippet: LRRK2 expression in colonic biopsy specimens from patients with CD. A, Paired biopsy specimens from intestinal tissues of patients with IBD (inactive disease state) were collected: one specimen from an inflamed area and one from a control noninflamed area for each patient. Total mRNA was extracted and used for qRT-PCR. The amount of LRRK2 mRNA relative to GAPDH mRNA of each inflamed biopsy specimen was normalized to the quantity measured in the corresponding noninflamed biopsy specimen obtained from the same patient. Statistical analyses were performed using Student t test (*p < 0.05). B and C, Frozen sections (7 μm) were prepared from colonic biopsy specimens obtained from patients with IBD. Tissues were immunolabeled with rabbit anti-LRRK2 (clone 267) and rat anti–troma-1 Abs to detect intestinal epithelial cells. The image gallery in B was performed with anti-LRRK2 Abs preincubated or not with its corresponding blocking peptide. C, Mouse monoclonal anti-CD3, anti-CD19, anti-myeloperoxidase, anti-mannose receptor (MNR), and anti-CD103 Abs were also used to detect T-lymphocytes, B-lymphocytes, neutrophils, macrophages, and dendritic cells, respectively. After immunostaining using secondary FITC-anti–mouse, Cy3-anti–rabbit and Cy5-anti–rat species-specific Abs, fluorescence was detected by confocal microscopy. The image gallery displays single confocal sections (original magnification ×60). The bottom panel arrows indicate the cells containing signals from both LRRK2 and FITC-MNR, CD103, CD20, and CD3 cell specific markers, respectively.

Article Snippet: Anti-CD20 (L26) Ab was obtained from ScyTek Laboratories (Logan, UT).

Techniques: Expressing, Control, Quantitative RT-PCR, Immunolabeling, Blocking Assay, Immunostaining, Fluorescence, Confocal Microscopy

Journal: Scientific Reports

Article Title: Association between nonspecific interstitial pneumonia and presence of CD20+ B lymphocytes within pulmonary lymphoid follicles

doi: 10.1038/s41598-017-17208-1

Figure Lengend Snippet: Distribution of lymphocytes in different anatomic regions of lung tissue. CD20+ and CD14+ lymphocytes mainly infiltrated the lymphoid follicle regions. H&E, haematoxylin and eosin.

Article Snippet: The paraffin sections were immunohistochemically stained manually with anti-CD14 antibody (clone EP128; Zeta Corp., Sierra Madre, CA, dilution 1:100) and anti-CD20 antibody (clone L26; Zeta Corp., dilution 1:100).

Techniques:

Journal: Scientific Reports

Article Title: Association between nonspecific interstitial pneumonia and presence of CD20+ B lymphocytes within pulmonary lymphoid follicles

doi: 10.1038/s41598-017-17208-1

Figure Lengend Snippet: Distribution of CD20+ and CD14+ lymphocytes in different anatomic regions.

Article Snippet: The paraffin sections were immunohistochemically stained manually with anti-CD14 antibody (clone EP128; Zeta Corp., Sierra Madre, CA, dilution 1:100) and anti-CD20 antibody (clone L26; Zeta Corp., dilution 1:100).

Techniques:

Journal: Scientific Reports

Article Title: Association between nonspecific interstitial pneumonia and presence of CD20+ B lymphocytes within pulmonary lymphoid follicles

doi: 10.1038/s41598-017-17208-1

Figure Lengend Snippet: Distribution of CD20+ and CD14+ lymphocytes in different pathologic subtypes of NSIP.

Article Snippet: The paraffin sections were immunohistochemically stained manually with anti-CD14 antibody (clone EP128; Zeta Corp., Sierra Madre, CA, dilution 1:100) and anti-CD20 antibody (clone L26; Zeta Corp., dilution 1:100).

Techniques:

Journal: Scientific Reports

Article Title: Association between nonspecific interstitial pneumonia and presence of CD20+ B lymphocytes within pulmonary lymphoid follicles

doi: 10.1038/s41598-017-17208-1

Figure Lengend Snippet: Relationship between CD20+ lymphocytes and changes in FVC after 1 year of treatment.

Article Snippet: The paraffin sections were immunohistochemically stained manually with anti-CD14 antibody (clone EP128; Zeta Corp., Sierra Madre, CA, dilution 1:100) and anti-CD20 antibody (clone L26; Zeta Corp., dilution 1:100).

Techniques:

Journal: Scientific Reports

Article Title: Association between nonspecific interstitial pneumonia and presence of CD20+ B lymphocytes within pulmonary lymphoid follicles

doi: 10.1038/s41598-017-17208-1

Figure Lengend Snippet: Negative relationship between follicular CD20+ B cells and percentage change in forced vital capacity after 12 months of therapy. FVC, forced vital capacity.

Article Snippet: The paraffin sections were immunohistochemically stained manually with anti-CD14 antibody (clone EP128; Zeta Corp., Sierra Madre, CA, dilution 1:100) and anti-CD20 antibody (clone L26; Zeta Corp., dilution 1:100).

Techniques:

Journal: Scientific Reports

Article Title: Association between nonspecific interstitial pneumonia and presence of CD20+ B lymphocytes within pulmonary lymphoid follicles

doi: 10.1038/s41598-017-17208-1

Figure Lengend Snippet: Relationship between CD20+ lymphocytes and CT improvements.

Article Snippet: The paraffin sections were immunohistochemically stained manually with anti-CD14 antibody (clone EP128; Zeta Corp., Sierra Madre, CA, dilution 1:100) and anti-CD20 antibody (clone L26; Zeta Corp., dilution 1:100).

Techniques:

Journal: Veterinary Medicine and Science

Article Title: Disseminated Transmissible Venereal Tumour in the Mammary Glands of a Dog: A Histopathological and Immunohistochemical Case Report

doi: 10.1002/vms3.70229

Figure Lengend Snippet: Characteristics of antibodies used for the immunohistochemical technique.

Article Snippet: The sections were then incubated with primary antibodies, including vimentin (a marker showing cells of mesenchymal origin), cytokeratin (CK, a marker showing cells of epithelial origin), S‐100 protein (a marker for melanomas), CD3 (a marker for T lymphocytes), CD20 (a marker for B lymphocytes), CD68 (a marker for macrophages), Ki‐67 (a marker for cell proliferation), c‐Myc (a marker for cell proliferation) and p53 (a marker for apoptosis) (all antibodies from Master Diagnostica, Granada, Spain) overnight at 4°C (Table ).

Techniques: Immunohistochemical staining, Positive Control, Staining

Journal: Frontiers in Oncology

Article Title: Predictive model based on multiple immunofluorescence quantitative analysis for pathological complete response to neoadjuvant immunochemotherapy in lung squamous cell carcinoma

doi: 10.3389/fonc.2024.1396439

Figure Lengend Snippet: Logistic regression univariate and multivariate analysis.

Article Snippet: The mIF staining, which was completed at Genecast Biotechnology Co., Ltd., detected two panels comprising a total of 10 markers: CD4 (EP204), CD8 (SP16), PD-L1 (SP142), TIM3 (EPR22241), and LAG-3 (EPR20261) in panel 1; and CD3 (LN10), CD20 (L26), CD21 (EP3093), CD163 (10D6), and Foxp3 (236A/E7) in panel 2.

Techniques:

Journal: Scientific Reports

Article Title: Semi-automated approaches for interrogating spatial heterogeneity of tissue samples

doi: 10.1038/s41598-024-55387-w

Figure Lengend Snippet: Details on biomarker panel, image acquisition and data used for analysis.

Article Snippet: CD20 , TRITC , L26, Lunaphore , Cytoplasm , 40,000.

Techniques: Biomarker Discovery, Marker, Fluorescence