Journal: Molecules (Basel, Switzerland)

Article Title: Effect of Supplementation with the Combination of Se-Enriched Lentinula edodes Mycelium, Exogenous Enzymes, Acidifiers, Sodium Butyrate and Silicon Dioxide Nanoparticle Feed Additives on Selected Parameters in Calves.

doi: 10.3390/molecules27165163

Figure Lengend Snippet: Figure 2. Percentage of the lymphocyte (LYM) subpopulations containing surface antigens in the peripheral blood of experimental (E) and control (C) calves: (a) CD2+; (b) CD4+; (c) CD8+; (d) WC1+; (e) MHC class II, and (f) percentage of the monocyte (MON) subpopulation containing CD11b+

Article Snippet: The following mouse anti-bovine monoclonal antibodies (mAb) with fluorescein isothiocyanate (FITC) were used: CD2+, CD4+, CD8+, WC1+, MHC class II monomorphic, and CD11b+ (Bio-Rad, Hercules, USA).

Techniques: Control

Journal: Academic radiology

Article Title: Natural Killer Cell-Associated Radiogenomics Model for Hepatocellular Carcinoma: Integrating CD2 and Enhanced CT-Derived Radiomics Signatures.

doi: 10.1016/j.acra.2024.10.043

Figure Lengend Snippet: Figure 3. Identification and validation of CD2 an NK cell-related prognostic biomarker for HCC patients. (a) Selection of the λ parameter in LASSO-Cox regression. Vertical lines indicate partial likelihood deviance for each λ value, with the minimum deviance at an optimal λ of 0.05. (b) Process of deriving the four candidate genes with non-zero coefficients at the optimal λ value. The vertical axis shows the regression coefficients, and the horizontal axis shows the log(λ) values. (c) Forest plot of multivariable Cox regression analysis, confirming CD2 as an independent prognostic factor among the four selected genes. Kaplan–Meier analysis of the TCGA-LIHC cohort. High CD2 expression is significantly associated with longer (d) OS and (e) PFS. (f) Kaplan–Meier analysis of the GSE76427 cohort, showing that high CD2 expression is significantly associated with longer OS. Kaplan–Meier analysis of the GSE14520 cohort. High CD2 expression is significantly associated with longer (g) OS and (h) RFS. (i) Kaplan–Meier analysis of the TCIA-TCGA-LIHC cohort, showing that high CD2 expression is significantly associated with longer OS. (j) Ridgeline plot illustrating GSEA results, highlighting significant positive correlations between CD2 expression and KEGG processes. (k) GSEA indicated that high CD2 expression was enriched in NK cell-mediated cytotoxicity signaling. HCC, Hepatocellular carcinoma; KEGC, Kyoto Encyclopedia of Genes and Genomes; LASSO, Least absolute shrinkage and selection operator; NK, Natural killer; RFS, Recurrence-free survival; TCGA-LHIC, The Cancer Genome Atlas-Liver Hepatocellular Carcinoma.

Article Snippet: Sections were then incubated overnight at 4 °C with a primary rabbit monoclonal antibody against CD2 (ABclonal, China, A23199, 1:200 dilution).

Techniques: Biomarker Discovery, Selection, Expressing

Journal: Academic radiology

Article Title: Natural Killer Cell-Associated Radiogenomics Model for Hepatocellular Carcinoma: Integrating CD2 and Enhanced CT-Derived Radiomics Signatures.

doi: 10.1016/j.acra.2024.10.043

Figure Lengend Snippet: Figure 4. Radiomics model construction and validation. (a) Representative enhanced CT arterial phase image of a patient with HCC, with the white arrow indicating the location of the tumor. (b) ROI outlining, with the green area showing the ROI, and the 3D image of the ROI displayed in the upper-right corner. (c) Selection of the λ parameter for the LASSO-Cox model. The vertical lines indicate partial likelihood deviance for each λ value, with the optimal λ at 0.12. (d) Use of the optimal value of λ to select three features. (e, g, i) Kaplan–Meier analysis of OS based on radioscore stratification in the TCIA-TCGA-LIHC, TCIA-TACE-seg, and Tongji cohorts. (f, h, j) Distribution of radioscores, survival time, survival status, and heatmaps of selected radiomics features for each cohort. (k) Representative immunohistochemistry images for CD2 expression in samples from the Tongji cohort. (l) Differences in radioscores among HCC patients with different TNM or BCLC stages across the three cohorts. HCC, Hepatocellular carcinoma; LASSO, Least absolute shrinkage and selection operator; OS, Overall survival; TCIA-TCGA-LIHC, The Cancer Imaging Archive-The Cancer Genome Atlas-Liver Hepatocellular Carcinoma; TACE, Transarterial chemoembolization .

Article Snippet: Sections were then incubated overnight at 4 °C with a primary rabbit monoclonal antibody against CD2 (ABclonal, China, A23199, 1:200 dilution).

Techniques: Biomarker Discovery, Selection, Immunohistochemistry, Expressing, Imaging

Journal: Academic radiology

Article Title: Natural Killer Cell-Associated Radiogenomics Model for Hepatocellular Carcinoma: Integrating CD2 and Enhanced CT-Derived Radiomics Signatures.

doi: 10.1016/j.acra.2024.10.043

Figure Lengend Snippet: Figure 5. Overview of the comparison of the four HCC radiogenomics subtypes defined by CD2 expression and radioscore. (a) Sankey diagram of samples distribution in four radiogenomics subtypes based on CD2 expression and radioscore. (b) Kaplan–Meier analysis of OS in the four radiogenomics subgroups of patients with HCC patients. p values for comparisons between each pair of groups are shown in the upper left corner. (c) KEGG enrichment analysis of the overlapping genes from the differential gene expression analysis of the high CD2 plus low radioscore subgroup and the other three subgroups. (d) Heatmap of GSVA results for NK cell-related pathways and biological processes for each radiogenomics subgroup. (e) Mutation waterfall plot of the top 20 genes with the highest mutation frequency in the patients of the four subgroups. GSVA, Gene Set Variation Analysis; KEGC, Kyoto Encyclopedia of Genes and Genomes; NK, Natural killer.

Article Snippet: Sections were then incubated overnight at 4 °C with a primary rabbit monoclonal antibody against CD2 (ABclonal, China, A23199, 1:200 dilution).

Techniques: Comparison, Expressing, Gene Expression, Mutagenesis

Journal: Cells

Article Title: A Mouse Model to Study the Pathogenesis of γ-herpesviral Infections in Germinal Center B Cells.

doi: 10.3390/cells12242780

Figure Lengend Snippet: Figure 5. Virally induced constitutively active CD30 signaling results in more Cre-reporter+ cells within B cells. CAR//Cγ1-cre mice (dots) and LC30//Cγ1-cre mice (squares) were i.p. infected with MHV-68 hNGFR and splenic cells were analyzed 14 days post-infection. (A) Breeding scheme of LC30//Cγ1cre mice. The excision of the loxP-flanked stop cassette was followed by hCD2 reporter expression (Cre-reporter) and ligand-independent constitutively active CD30 signaling. (B) The splenic

Article Snippet: The CD19-PeVio770 (REA749), CD38-PeVio770 and human CD2 (hCD2)-PeVio770 (LT2) antibodies were all purchased from Miltenyi Biotech (Bergisch Gladbach, Germany).

Techniques: Infection, Expressing

Journal: bioRxiv

Article Title: Ångström Resolution with Flow Immunofluorescence Localization Microscopy (FILM)

doi: 10.64898/2025.12.16.694546

Figure Lengend Snippet: (A–C) FILM acquisition of binder probes that recognize CD2, ICAM-1 (CD54) in the pSMAC, and CD45 in the dSMAC zones (10 nm pixels with 3-pixel Gaussian blurring). For CD2 and CD45, APC-conjugated antibodies were used; for ICAM-1, a nanobody Fab conjugated with Vio ® 667 was used. Cyan boxed region shows the IF image (103.17 nm pixels) corresponding to the white boxed region. (D–F) Zoom-in of the respective boxed regions for the panels to the left. Localizations are shown as peak-normalized 2D Gaussians with color-coded precision according to the colorbar. (G–I) Zoom-in of the respective boxed regions for the panels to the immediate left. Localizations are shown as crosses (indicating ±1-σ uncertainty in x and y ) with color-coded precision according to the colorbar.

Article Snippet: α-tubulin (REA1136 REAfinityTM, h, APC, #130-119-541, Miltenyi Biotec), CD2 (REA972 REAfinityTM, h, APC, #130-116-150, Miltenyi Biotec), CD20 (REA780 REAfinityTM, h, APC, #130-111-339, Miltenyi Biotec), CD28 (REA612 REAfinityTM, h, APC, #130-118-343, Miltenyi Biotec), CD45 (REA1023 REAfinityTM, h/nhp, APC, #130-117-191, Miltenyi Biotec), CD54/ICAM-1 (REA266 REAfinityTM, h, APC, #130-120-711, Miltenyi Biotec), CD81 (REA513 REAfinityTM, h, APC, #130-119-787, Miltenyi Biotec), CD107a/LAMP1 (REA792 REAfinityTM, h, APC, #130-111-847, Miltenyi Biotec), IgD (REA740 REAfinityTM, h, APC, #130-110-644, Miltenyi Biotec), IgM (REAL689 REAdye_leaseTM, h, PE, #130-125-786, Miltenyi Biotec), Pan-Cytokeratin (REA1141 REAfinityTM, h, APC, #130-120-096, Miltenyi Biotec), β-catenin (REA480 REAfinityTM, h, APC, #130-124-444, Miltenyi Biotec), TCRα/β (REA652 REAfinityTM, h, APC, #130-113-535, Miltenyi Biotec), TOM22 (REA1185 REAfinityTM, APC, h/ms, #130-122-079, Miltenyi Biotec), DAPI Staining Solution (#130-111-570, Miltenyi Biotec), and ATTO 643 phalloidin (ATT-AD643-81, ATTO-TEC).

Techniques: