Journal: Development (Cambridge, England)

Article Title: Synchronous and symmetric migration of Drosophila caudal visceral mesoderm cells requires dual input by two FGF ligands.

doi: 10.1242/dev.068791

Figure Lengend Snippet: Fig. 3. FGF signaling supports CVM cell survival. Embryos are oriented with anterior towards the left and dorsal upwards. (A-C)Embryos containing the croc-lacZ reporter gene stained using anti-bgal (red) and anti-FasIII (green) to identify CVM and TVM cells, respectively. Wild-type (stage 13; A), Df(2R)BSC25 (stage 13; B) and Df(2R)BSC25 embryos expressing p35 via G447.Gal4 driver (stage 13; C) are depicted. (C)Expression of the anti-apoptotic protein p35 rescues the morphology of CVM cells and allows cells to migrate anteriorly, but cells (asterisk) fail to reach the anterior-most position of the TVM (white line). (D-F)Cell death is observed in FGF mutants using the TUNEL assay. Wild-type embryo (i.e. G447.Gal4 UAS-CD2) of stage 13 (D) and Df(2R)BSC25 G447.Gal4 UAS-CD2 embryos of stage 12 (E) and stage 13 (F) stained with anti-CD2 to detect CVM cells (red) and apoptosis using the TUNEL assay (green).

Article Snippet: The following antibodies were used in the study: rabbit anti-b-gal antibody (1:400; Molecular Probes), mouse anti-Fas III antibody (1:10; Developmental Studies Hybridoma Bank), mouse anti-CD2 antibody (1:300; Serotec), mouse anti-bio (1:500; Roche) and sheep antidig (1:500; Roche).

Techniques: Staining, Expressing, TUNEL Assay

Journal: International immunopharmacology

Article Title: Minnelide combined with Angptl3 knockout completely protects mice with adriamycin nephropathy via suppression of TGF-β1-Smad2 and p53 pathways.

doi: 10.1016/j.intimp.2022.109656

Figure Lengend Snippet: Fig. 2. Minnelide alleviates podocyte injury in Angptl3 knockout mice with adriamycin nephropathy. (A) Immunofluorescence of nephrin, podocin, and cd2ap in kidney tissues of three groups of mice. (B and C) The protein expression levels of nephrin, podocin, and cd2ap in kidney tissues of three groups of mice were measured by western blot. n = 3, ****P < 0.0001 vs Angptl3-/- group; #P < 0.05, ##P < 0.01 and ####P < 0.0001 vs ADR + Angptl3-/- group.

Article Snippet: The membrane was incubated overnight at 4 ◦C with the following primary antibodies: nephrin (1:500, Invitrogen, PA5-106921), podocin (1:500, Proteintech, 20384–1-AP), cd2ap (1:500, Invitrogen, PA5-51894), α-SMA (1:1000, Servicebio, GB111364), TGF-β1 (1:500, Servicebio, GB111876), pSmad2 (1:500, Servicebio, GB11343), pp53 (1:500, Servicebio, B. Ji et al. International Immunopharmacology 115 (2023) 109656 GB114061), p53 (1:500, Proteintech, 10442–1-AP), Bax (1:500, Abcam, ab216494), Bcl-2 (1:500, Abcam, ab196495), and Cleaved-caspase3 (1:500, Servicebio, GB11532).

Techniques: Knock-Out, Immunofluorescence, Expressing, Western Blot

Journal: International immunopharmacology

Article Title: Minnelide combined with Angptl3 knockout completely protects mice with adriamycin nephropathy via suppression of TGF-β1-Smad2 and p53 pathways.

doi: 10.1016/j.intimp.2022.109656

Figure Lengend Snippet: Fig. 8. Triptolide attenuates podocyte injury in Angptl3 knockout primary podocytes. (A) Immunofluorescence of nephrin, podocin, and cd2ap in Angptl3 knockout primary podocytes. (B) The expression of podocin and cd2ap proteins in Angptl3 knockout primary podocytes was measured by western blot. n = 3. ****P < 0.0001 vs Angptl3-/- group; ##P < 0.01 and ####P < 0.0001 vs ADR + Angptl3-/- group.

Article Snippet: The membrane was incubated overnight at 4 ◦C with the following primary antibodies: nephrin (1:500, Invitrogen, PA5-106921), podocin (1:500, Proteintech, 20384–1-AP), cd2ap (1:500, Invitrogen, PA5-51894), α-SMA (1:1000, Servicebio, GB111364), TGF-β1 (1:500, Servicebio, GB111876), pSmad2 (1:500, Servicebio, GB11343), pp53 (1:500, Servicebio, B. Ji et al. International Immunopharmacology 115 (2023) 109656 GB114061), p53 (1:500, Proteintech, 10442–1-AP), Bax (1:500, Abcam, ab216494), Bcl-2 (1:500, Abcam, ab196495), and Cleaved-caspase3 (1:500, Servicebio, GB11532).

Techniques: Knock-Out, Immunofluorescence, Expressing, Western Blot