Journal: Stem Cell Research & Therapy

Article Title: Characterization and angiogenic potential of CD146 + endometrial stem cells

doi: 10.1186/s13287-024-03918-7

Figure Lengend Snippet: A variety of cell surface markers are employed for the marker study of eSCs. Conjugated antibodies were used to conduct the experiment. The antibody concentrations were adjusted according to the specific recommendation for each antibody

Article Snippet: 17. , CD19 PE conjugate , IgG1 , Mouse , 10 μL/10^6 cells , R&D Systems, FAB4867P.

Techniques: Marker

Journal: Stem Cell Research & Therapy

Article Title: Characterization and angiogenic potential of CD146 + endometrial stem cells

doi: 10.1186/s13287-024-03918-7

Figure Lengend Snippet: Isolation and culturing eSCs, surface marker analysis and growth factor analysis Figure 1 A ; The sample was collected from the hospital (i), trypsin was used to digest it (ii), centrifugation was used to obtain the cell pellet (iii), and the isolated cells were cultured in full DMEM media (iv) image is taken at 10X. Figure 1 B ; The endometrial cells showed remarkable expression of mesenchymal (CD90, CD105, CD73, CD140b, CD146), cell adhesion molecules (CD29, CD44, CD166). However, the expression of HLA-DR, CD34, CD45, CD14 and CD19 were negative. Figure 1 C ; In growth factor secretion profile GM-CSF, G-CSF secretion concentration was higher than others. Followed by EGF, FGF basic, PDGF-AA and VEGF ( p value: <0.0001)

Article Snippet: 17. , CD19 PE conjugate , IgG1 , Mouse , 10 μL/10^6 cells , R&D Systems, FAB4867P.

Techniques: Isolation, Marker, Centrifugation, Cell Culture, Expressing, Concentration Assay

Journal: Molecular therapy. Methods & clinical development

Article Title: Selective B cell depletion upon intravenous infusion of replication-incompetent anti-CD19 CAR lentivirus.

doi: 10.1016/j.omtm.2022.05.006

Figure Lengend Snippet: Figure 3. Immunophenotyping of 1D3-CD19CAR- GFP (blue, circle), FMC63-CD19CAR-GFP (red, square), or control (GFP only, green, triangle) trans- duced T cells in peripheral blood of treated mice Values shown are the percentage of total CD3+, CD90.2+

Article Snippet: In order to verify the ability for both the 1D3-and FMC63-CD19CAR to target the mouse CD19 antigen, the A20 cells were incubated with 10, 1, 0.1, 0.001, or 0 mg/mL of either the 1D3CD19-blocking antibody (152402, BioLegend) or the FMC63-CD19- Molecular Th blocking antibody (NBP2-52716, Novus Biologicals).

Techniques: Control