Journal: Acta biomaterialia

Article Title: In vivo short-term and long-term host reaction to starch-based scaffolds.

doi: 10.1016/j.actbio.2010.06.020

Figure Lengend Snippet: Fig. 2. Micrographs of the sections of the explanted FB and WS produced starch-based scaffolds after 1 (A–D) and 2 (E–H) weeks of IM implantation in rats. Tissue was immunohistochemically labelled for CD18 and CD3. represents the SPCL scaffold fibres, arrows mark CD18-positive cells and dashed arrows mark CD3-positive cells.

Article Snippet: The histological analysis of crosssection samples was performed after haematoxylin and eosin (HE), and Masson Goldner Trichrome (MGT) staining and immunohistochemistry using a monoclonal mouse anti-human CD3 antibody (Dako, Denmark) with cross-reactivity for rat T lymphocytes, a monoclonal mouse anti-rat CD18 antibody (Serotec, UK) for the integrin b2 chain of recruited leukocytes, and a monoclonal mouse anti-human phosphoinositide 3-Kinase (Pi3K) antibody (BD, Belgium) with cross-reactivity for rat activated and proliferating lymphocytes, following standard protocols.

Techniques: Produced, Starch

Journal: Acta biomaterialia

Article Title: In vivo short-term and long-term host reaction to starch-based scaffolds.

doi: 10.1016/j.actbio.2010.06.020

Figure Lengend Snippet: Fig. 5. Micrographs of the sections of the explanted FB and WS produced starch-based scaffolds after 1 (A–D) and 2 (E–H) weeks of SC implantation in rats. Tissue was immunohistochemically labelled for CD18 and CD3. represents the SPCL scaffold fibres, arrows mark CD18-positive cells and dashed arrows mark CD3-positive cells.

Article Snippet: The histological analysis of crosssection samples was performed after haematoxylin and eosin (HE), and Masson Goldner Trichrome (MGT) staining and immunohistochemistry using a monoclonal mouse anti-human CD3 antibody (Dako, Denmark) with cross-reactivity for rat T lymphocytes, a monoclonal mouse anti-rat CD18 antibody (Serotec, UK) for the integrin b2 chain of recruited leukocytes, and a monoclonal mouse anti-human phosphoinositide 3-Kinase (Pi3K) antibody (BD, Belgium) with cross-reactivity for rat activated and proliferating lymphocytes, following standard protocols.

Techniques: Produced, Starch

Journal: Cell Communication and Signaling : CCS

Article Title: Neutrophil membrane-coated circular RNA nanoparticles for targeted immunotherapy in HER2-positive breast cancer brain metastasis

doi: 10.1186/s12964-025-02321-w

Figure Lengend Snippet: Construction and Characterization of HER2-Targeted dHL-60 Cell Membranes. (A) Schematic representation of the lentiviral vector design used to overexpress the scFv targeting HER2. The vector includes the anti-HER2 scFv, a membrane localization signal, a FLAG tag for detection, a hinge region for flexibility, and a transmembrane domain to anchor the scFv to the cell membrane. (B) SPR analysis illustrating the binding affinity of scFv to the HER2 protein. (C) Flow cytometry analysis comparing the binding of scFv (anti-HER2) to SKBR3 cells versus a control scFv. (D) Flow cytometry assessment of CD11b expression levels in HL-60 cells before and after differentiation induction with DMSO. (E) Western blot results demonstrating the expression of adhesion molecules CD11b, CD18, CD49d, and CD31 in differentiated HL-60 cells. (F) Western blot analysis was performed using an anti-FLAG antibody to detect the FLAG-tagged scFv (anti-HER2). A band corresponding to the scFv-FLAG fusion protein is observed in dHL-60 cells transduced with the lentiviral vector, but not in control cells. (G) Flow cytometry analysis (anti-FLAG antibody) demonstrating the surface expression of scFv (anti-HER2) on dHL-60 cells overexpressing scFv (anti-HER2). (H) Illustration depicting the construction and extraction process of the cell membrane from HL-60 cells, outlining the steps involved in preparing membrane-coated nanoparticles

Article Snippet: CoraLite ® Plus 488, PE-conjugated Goat Anti-Rabbit IgG, antibodies against CD11B, CD49d, CD31, CD18, β-actin, His Tag, FLAG Tag and CXCL9 were obtained from Proteintech (Wuhan, China).

Techniques: Plasmid Preparation, Membrane, FLAG-tag, Binding Assay, Flow Cytometry, Control, Expressing, Western Blot, Transduction, Extraction

Journal: Cell reports

Article Title: PAI-1 uncouples integrin-β1 from restrain by membrane-bound β-catenin to promote collagen fibril remodeling in obesity-related neoplasms

doi: 10.1016/j.celrep.2024.114527

Figure Lengend Snippet:

Article Snippet: For DKK1-, SPARC-, TIMP2-, COL1, integrin β2, or integrin α3 PLA, purified rabbit monoclonal anti-DKK1 (1:200, Cell Signaling Technology, Cat# 48367S), rabbit polyclonal anti-SPARC (1:200, Cell Signaling Technology, Cat# 5420S), mouse monoclonal anti-TIMP2 (1:200, R&D Systems, Cat# MAB971), mouse monoclonal anti-COL1 (1:200, Cell Signaling Technology, Cat# 66948S), goat polyclonal anti-integrin β1 (1:200, R&D Systems, Cat# AF1778), goat polyclonal anti-integrin β2 (1:200, R&D Systems, Cat# AF1730), and mouse monoclonal anti-integrin α3 (1:200, R&D Systems, Cat# MAB1345) were used.

Techniques: Recombinant, Membrane, Cell Culture, Reverse Transcription, SYBR Green Assay, Clinical Proteomics, Protein Extraction, Extraction, Bicinchoninic Acid Protein Assay, In Situ, Blocking Assay, Migration, shRNA, Control, Software, Pyromark Assay, Western Blot, Simple Western

Journal: Frontiers in Veterinary Science

Article Title: Flow cytometric-based detection of CD80 is a useful diagnostic marker of acute myeloid leukemia in dogs

doi: 10.3389/fvets.2024.1405297

Figure Lengend Snippet: Conjugated antibodies used for triple-labeling cells in acute leukemia immunophenotyping panels after April 2021, including their target antigen and registry number (when available) or source.

Article Snippet: CD11b-FITC/CD34-PE/CD18-A647* , CD18 : Bio-Rad Cat# MCA503A647, RRID:AB_324799.

Techniques:

Journal: Fundamental Research

Article Title: A dynamic peripheral immune landscape during human pregnancy

doi: 10.1016/j.fmre.2022.06.011

Figure Lengend Snippet: Aberrant cell-cell communication supported the decreased adaptive immune response after pregnancy. (a) Dot plot showing the representative GO biological process and pathways enriched in downregulated DEGs in DC and MC subsets. (b) Venn plot showing the integrated comparative analysis of downregulated DEGs among DC and MC subsets. (c) Circle plot showing the inferred MHC-II signaling networks. (d) Violin plot showing the expression of genes related to MHC-II signaling among immune populations between HC (blue) and Preg (red) groups. (e) Circle plot showing the inferred CD40 signaling networks. (f) Circle plot showing the inferred ICOS signaling networks in Preg group but absent in HC group. (g) Violin plot showing the expression of genes related to ICOS signaling among immune populations between HC (blue) and Preg (red) groups. (h) Circle plot showing the inferred MIF signaling networks. (i) Violin plot showing the expression of genes related to MIF signaling among immune populations between HC (blue) and Preg (red) groups. (j) The flow cytometry histogram showing the expression of CD74 in CD4 + TCs. Box plots showing the ratio of CD74-expressing CD4 + TCs between HC and Preg groups. (k) Violin plot showing the expression of genes related to ICAM signaling among immune populations between HC (blue) and Preg (red) groups. (l) The flow cytometry histogram showing the expression of CD18 in CD4 + TCs (left). Box plots showing the Mean Fluorescence Intensity (MFI) of CD18 in CD4 + TCs between HC and Preg groups (right). Significance in j, l was calculated using an unpaired, two-tailed Student's t-test; * P < 0.05.

Article Snippet: For the surface staining, PBMCs were simultaneously stained with anti-human fluorochrome-labeled antibodies specific for CD45 (BV785, #304048), CD3 (BV421, #300434), CD14 (PerCP/Cy5.5, #301824), CD4 (APC, #317416 or PE, #300508), CD8 (PerCP/Cy5.5, #301031), CD45RA (BV785, #304140), CD45RO (BV605, #304238), CD1C (PerCP/Cy5.5, #331514), CD19 (BV605, #302244), CD20 (APC, #302310), CD38 (BV785, #303530), HLA-DQ (PE, #318105), BCMA (PE-Cy7, #357508), CD74 (APC, #326811), CD69 (BV650, #310934), CD83 (PE, #305307) (BioLegend, San Diego, USA) and CD18 (APC, #E-AB-F1057E) (Elabscience).

Techniques: Expressing, Flow Cytometry, Fluorescence, Two Tailed Test