Journal: Parasite immunology

Article Title: SEA Alleviates Hepatic Ischaemia-Reperfusion Injury by Promoting M2 Macrophage Polarisation.

doi: 10.1111/pim.13061

Figure Lengend Snippet: FIGURE 4 | SEA pretreatment promoted the polarisation of M2 macrophages. Mice were subjected to 60 min of hepatic ischaemia, followed by 6 h of reperfusion. (A) Representatives of immunofluorescence staining of CD68 (n = 5 per group, five biological replicates from five individual animals; original magnification 100× and 200×, scale bar: 100 μm). (B) Representatives of immunofluorescence staining of CD163 (n = 5 per group, five biological replicates from five individual animals; original magnification 100× and 200×, scale bar: 100 μm). (C, D) The expression of M2 macrophage marker (CD206 and Arg1) in the liver was detected via qRT-PCR (n = 5 per group, five biological replicates from five individual animals). These results were obtained from at least three independent experiments. Values are presented as mean ± SEM. **p < 0.01, *p < 0.05, ns > 0.05.

Article Snippet: Then the sections were incubated with primary antibodies dissolved in 5% donkey serum solution containing 0.3% Triton at 4°C overnight (anti- MPO, Cat# ab208670, Abcam, 1:100; anti- CD68, Cat# ab53444, Abcam, 1:100; anti- CD163, Cat# 16646- 1- AP, Proteintech, 1:100).

Techniques: Immunofluorescence, Staining, Expressing, Marker, Quantitative RT-PCR

Journal: Acta biomaterialia

Article Title: A subtype specific probe for targeted magnetic resonance imaging of M2 tumor-associated macrophages in brain tumors.

doi: 10.1016/j.actbio.2025.01.003

Figure Lengend Snippet: Fig. 6. Immunohistochemical and FACS validations of M2pep-uIONP targeting M2 TAMs. Confocal microscopic images of tumor tissues collected from mice at 24 h after i.v. injection of probes show (A) Cy7-M2pep-uIONP were highly co-localizing with CD68- and CD163-double positive M2 TAM, revealing the targeting specificity. In comparison, the non-targeted control probe Cy7-scM2pep-uIONP and Cy7-Ferumoxytol not only exhibited much less tumoral accumulation, but also were not co-localized with M2 TAM. Scale bars: 37 µm. (B) Flow cytometric zebra plots and (C) the corresponding quantification of CD68- and CD163-double positive M2 TAM targeted by FITC-M2pep-uIONP, FITC-scM2pep-uIONP, and FITC-Ferumoxytol. The gating strategy involves sorting CD163+ M2 TAM population in live cells dissociated from mouse cerebrums by gating on CD68+ pan-macrophage populations, followed by further gating to assess the FITC-labeled nanoparticles.

Article Snippet: Rabbit polyclonal anti-CD163 antibody was purchased from Boster Biological Technology (Pleasanton, CA, USA).

Techniques: Immunohistochemical staining, Injection, Comparison, Control, Labeling

Journal: OncoImmunology

Article Title: Detailed resolution analysis reveals spatial T cell heterogeneity in the invasive margin of colorectal cancer liver metastases associated with improved survival

doi: 10.1080/2162402x.2017.1286436

Figure Lengend Snippet: Figure 5: Boxplots of identified CD8, GranB, CD163 and CD20 cell densities in different distance classes. Statistically

Article Snippet: Mouse monoclonal antibodies recognizing human CD3 (1:50 dilution, clone PS1, Acris #DM112-05), CD8 (1:50 dilution, clone 4B11, Novocastra #NCL-CD8-4B11), CD20 (1:50 dilution, clone L26, Novocastra #NCL-L-CD20-L26), GranB (1:20 dilution, clone 11F1, Novocastra #NCL-Gran-B) and human CD163 (1:500 dilution, clone EDHu-1, AbDSerotec #MCA1853) were applied as primary antibodies for 30 min at room temperature.

Techniques:

Journal: OncoImmunology

Article Title: Detailed resolution analysis reveals spatial T cell heterogeneity in the invasive margin of colorectal cancer liver metastases associated with improved survival

doi: 10.1080/2162402x.2017.1286436

Figure Lengend Snippet: Figure 7: Processing of serial section images for the quantification of distances between CD3 T cells and CD163

Article Snippet: Mouse monoclonal antibodies recognizing human CD3 (1:50 dilution, clone PS1, Acris #DM112-05), CD8 (1:50 dilution, clone 4B11, Novocastra #NCL-CD8-4B11), CD20 (1:50 dilution, clone L26, Novocastra #NCL-L-CD20-L26), GranB (1:20 dilution, clone 11F1, Novocastra #NCL-Gran-B) and human CD163 (1:500 dilution, clone EDHu-1, AbDSerotec #MCA1853) were applied as primary antibodies for 30 min at room temperature.

Techniques:

Journal: OncoImmunology

Article Title: Detailed resolution analysis reveals spatial T cell heterogeneity in the invasive margin of colorectal cancer liver metastases associated with improved survival

doi: 10.1080/2162402x.2017.1286436

Figure Lengend Snippet: Figure 8: Immunofluorescent double staining for CD163 (red) and PD-L1 (green) at the invasive margin. Dashed lines

Article Snippet: Mouse monoclonal antibodies recognizing human CD3 (1:50 dilution, clone PS1, Acris #DM112-05), CD8 (1:50 dilution, clone 4B11, Novocastra #NCL-CD8-4B11), CD20 (1:50 dilution, clone L26, Novocastra #NCL-L-CD20-L26), GranB (1:20 dilution, clone 11F1, Novocastra #NCL-Gran-B) and human CD163 (1:500 dilution, clone EDHu-1, AbDSerotec #MCA1853) were applied as primary antibodies for 30 min at room temperature.

Techniques: Double Staining