Journal: Advanced Science

Article Title: Exosome‐Mediated Lectin Pathway and Resistin‐MIF‐AA Metabolism Axis Drive Immune Dysfunction in Immune Thrombocytopenia

doi: 10.1002/advs.202412378

Figure Lengend Snippet: Landscape of BM mononuclear cells in patients with ITP. A). Uniform manifold approximation projection (UMAP) visualization of single‐cell transcriptional states obtained from BM mononuclear cells of patients with ITP compared with HDs. Distinct colors represent individual cell types determined based on gene expression: HSPC, hematopoietic stem and progenitor cells; T/NK, T and NK cells; pDC, plasmacytoid dendritic cells; DC, dendritic cells; Mono, monocytes. B). Analysis of proportions of different cell types in each sample. C). Selected lists of DE genes for ITP patient‐derived BMMC compared to HD‐derived BMMC were analyzed by GO analysis and annotated using GO terms. Graphs depicting selected GO terms, including complement‐associated genes. D). UMAP of BMMC showing the scores of “UCell: HALLMARK‐COMPLEMENT.” E). Bubble plot of complement pathway associated gene expression in myeloid population, including CD14 + CD16 − monocytes, CD16 + monocytes, and DC from patients with ITP versus healthy donors.

Article Snippet: [ ] Cells were incubated with different combinations of antibody mixtures (PE anti‐human CD284 (TLR4) Antibody (Clone No. HTA125, Cat. No. 312 805, BioLegend), APC Anti‐Human CD14 Antibody (Clone No. M5E2, Cat. No. E‐AB‐F1209E, Elabscience), APC/Cyanine7 anti‐human CD16 Antibody (Clone No. 3G8, Cat. No. 302 018, BioLegend), PE Anti‐Human CD16 Antibody (Clone No. 3G8, Cat. No. E‐AB‐F1236D, Elabscience), APC/Cyanine7 anti‐human CD46 Antibody (Clone No. TRA‐2‐10, Cat. No. 352 410, BioLegend), PE/Cyanine7 anti‐human CD88 (C5aR) Antibody (Clone No. S5/1, Cat. No. 344 308, BioLegend), Biotin anti‐human CD35 Antibody (Clone No. E11, Cat. No. 333 414, BioLegend), FITC anti‐human CD55 Antibody (Clone No. JS11, Cat. 311 306, BioLegend), PE anti‐human CD59 Antibody (Clone No. p282 (H19), Cat. 304 708, BioLegend), PerCP/Cyanine5.5 anti‐mouse/human CD11b Antibody (Clone No. M1/70, Cat. No. 101 228, BioLegend)), Biotin anti‐human CD11c Antibody (Clone No. Bu15, Cat. No. 337 232, BioLegend), APC anti‐human C3AR Antibody (Clone No. hC3aRZ8, Cat. No. 345 806, BioLegend), FITC anti‐human CD18 Antibody (Clone No. TS1/18, Cat. No. 302 106, BioLegend), Biotin anti‐human/mouse CXCR7 Antibody (Clone No. 8F11‐M16, Cat. No. 331 111, BioLegend), APC/Cyanine7 anti‐mouse/human CD44 Antibody (Clone No. IM7, Cat. No. 103 028, BioLegend), PE anti‐human CD184 (CXCR4) Recombinant Antibody (Clone No. QA18A64, Cat. No. 304 504, BioLegend), APC anti‐human CD74 Antibody (Clone No. LN2, Cat. No. 326 812, BioLegend) for 45 min in the dark at 4 °C.

Techniques: Gene Expression, Derivative Assay

Journal: Memórias do Instituto Oswaldo Cruz

Article Title: Concomitant immunity against superimposed homologous Echinostoma caproni infections in mice is mediated by interleuquin-25

doi: 10.1590/0074-02760250004

Figure Lengend Snippet: primary and superimposed infection induce different patterns of macrophage activation. (A) Indirect immunofluorescence with recombinant anti-CD16-2/FCGR4/Fc for M1 and antibody MR/CD206/Mannose Receptor for M2 (red) on intestinal tissue of Institute of Cancer Research (ICR) naïve and mice after Echinostoma caproni primary, superimposed infection in mice and superimposed infections in anti-interleuquin-25 (α-IL-25)-treated mice (scale bar: 100 mm). (B) Quantitation of the percentage of area covered by anti-CD16-2/FCGR4/Fc for M1 and antibody MR/CD206/Mannose Receptor staining. Vertical bars show standard deviation. a: significant differences with respect to negative controls; b: significant differences with respect to primary infections; c: significant differences with respect to superimposed infection- in α-IL-25-treated mice (p < 0.05).

Article Snippet: A rabbit anti-CD16-2/FCGR4/Fc antibody (Sino Biological, clone no. 012), and a goat antibody MR/CD206/Mannose Receptor (Novus Invitro Technologies Ltd, Colorado, USA) were used for labelling M1 and M2, respectively.

Techniques: Infection, Activation Assay, Immunofluorescence, Recombinant, Quantitation Assay, Staining, Standard Deviation