Journal: Cell reports

Article Title: PARP-inhibition reprograms macrophages toward an anti-tumor phenotype

doi: 10.1016/j.celrep.2022.111462

Figure Lengend Snippet:

Article Snippet: Anti-CD152 (CTLA-4)-154Sm , Fluidigm , Cat# 3154008B; clone: UC10-4B9.

Techniques: Control, Recombinant, Liposomes, Labeling, Quantitation Assay, Staining, RNA Sequencing, Mass Spectrometry, Knock-Out, Software

Journal: Experimental Hematology & Oncology

Article Title: Physical activity improves outcomes of combined lenvatinib plus anti-PD-1 therapy in unresectable hepatocellular carcinoma: a retrospective study and mouse model

doi: 10.1186/s40164-022-00275-0

Figure Lengend Snippet: Immunohistochemical staining and analysis of subcutaneous tumors. Immunohistochemical staining was conducted on subcutaneous tumor tissue microarray. H-score were calculated to quantify the expression levels of marker proteins. Representative images captured at 40 ×. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Foxp3 forkhead box protein p3, CTLA4 cytotoxic T-lymphocyte-associated protein 4, TIGIT T cell immunoreceptor with Ig and ITIM domains, TIM3 T-cell immunoglobulin mucin-3, VISTA V domain-containing Ig suppressor of T-cell activation

Article Snippet: Primary Abs used for immunohistochemical staining included: anti-CD4 Ab (#25229, CST), anti-CD8α Ab (#98941,CST), anti-Forkhead box protein p3 (Foxp3) Ab (ab253297, Abcam), anti-F4/80 Ab (#70076, CST), anti-CTLA4 Ab (A00020, Boster), anti-T cell immunoreceptor with Ig and immunoreceptor tyrosine-based inhibitory motif (ITIM) domains (TIGIT) Ab (A01962, Boster), anti-TIM3 Ab (ab241332, Abcam) and, anti-V domain-containing Ig suppressor of T-cell activation (VISTA) Ab (#54979, CST).

Techniques: Immunohistochemical staining, Staining, Microarray, Expressing, Marker, Activation Assay

Journal: PLoS ONE

Article Title: Characterization of the Leukocyte Response in Acute Vocal Fold Injury

doi: 10.1371/journal.pone.0139260

Figure Lengend Snippet: Monoclonal anti-pig specific antibodies and their labels.

Article Snippet: The following conjugated anti-pig antibodies were also used simultaneously: CD14 or SWC3 (myeloid cells) labeled to fluorescein isothiocyanate (FITC; Abd Serotec) and human CD152 (CTLA–4) fused to murine IgG2a Fc labeled with RPE (Ancell Corporation, Stillwater, MN), which denotes expression of co-stimulatory markers CD80 and CD86.

Techniques: Labeling