Journal:

Article Title: Topical Estrogen Accelerates Cutaneous Wound Healing in Aged Humans Associated with an Altered Inflammatory Response

doi:

Figure Lengend Snippet: Estrogen reduces the subpopulation of neutrophils expressing L-selectin. A: Representative FACS analysis of single-labeled neutrophils with L-selectin antibody. C, control; E3, estrogen 3 pg/ml, E30, estrogen 30 pg/ml; E300, estrogen 300 pg/ml. L-selectin cell population observed in the upper right quadrant at 10 2 (FL2) in control panel, and is largely absent after estrogen treatment for 12 hours. B: Reduction of L-selectin cell surface expression detected by FACS after estrogen treatment. n = 8. C, control. Gender had no effect on either parameter when male and female responses were assessed independently. *P < 0.05.

Article Snippet: Immunostaining was performed on 7-μm cryosections for fibronectin (a rabbit anti-human fibronectin antibody, 1:100; Pasteur, Lyon, France), collagen I (rabbit anti-human antibody, 1:20; Pasteur), neutrophils (mouse anti-human CD15; 1:10 dilution; Serotec, Oxford, UK), monocyte/macrophages (anti-human CD14; 1:20 dilution, Serotec) and estrogen receptor (mouse anti-human, 1:50; Oncogene Science, Cambridge, UK).

Techniques: Expressing, Labeling, Control

Journal:

Article Title: Topical Estrogen Accelerates Cutaneous Wound Healing in Aged Humans Associated with an Altered Inflammatory Response

doi:

Figure Lengend Snippet: Estrogen receptor localization in acute wounds. Quantification of estrogen receptor-positive (ER+) cells in specific subgroups at day 7 post-wounding showed a significant increase in ER+CD15+ (neutrophil) cells and decrease in ER+ CD14+ (monocyte) cells in the placebo versus estrogen-treated wounds. *P < 0.05, n = 5 per group. ER+ CD14− CD15− cells were considered to be fibroblasts on morphological grounds and were found to be increased (not significantly) in the estrogen-treated wounds. No gender differences were observed. The data represented graphically are that of the male groups.

Article Snippet: Immunostaining was performed on 7-μm cryosections for fibronectin (a rabbit anti-human fibronectin antibody, 1:100; Pasteur, Lyon, France), collagen I (rabbit anti-human antibody, 1:20; Pasteur), neutrophils (mouse anti-human CD15; 1:10 dilution; Serotec, Oxford, UK), monocyte/macrophages (anti-human CD14; 1:20 dilution, Serotec) and estrogen receptor (mouse anti-human, 1:50; Oncogene Science, Cambridge, UK).

Techniques:

Journal: eLife

Article Title: Neutrophil-mediated fibroblast-tumor cell il-6/stat-3 signaling underlies the association between neutrophil-to-lymphocyte ratio dynamics and chemotherapy response in localized pancreatic cancer: A hybrid clinical-preclinical study

doi: 10.7554/eLife.78921

Figure Lengend Snippet:

Article Snippet: Antibody , CD15 (164Dy, Human, monoclonal) , Fluidigm , 3164001B , 1:1000.

Techniques: Sequencing

Journal: Journal of translational medicine

Article Title: The promoting effect and mechanism of MAD2L2 on stemness maintenance and malignant progression in glioma.

doi: 10.1186/s12967-023-04740-0

Figure Lengend Snippet: Fig. 6 MAD2L2 affects the stemness of glioma cells. A In the MAD2L2 high-expression group, all six different stemness scores are higher compared to the low-expression group. B GSEA analysis demonstrates that MAD2L2 is associated with the activation of glioblastoma stem cell pathways. C The expression of MAD2L2 is higher in stem cells compared to non-stem cells. D Immunofluorescence of CD133 and NESTIN in U87-derived glioblastoma stem cells. E After MAD2L2 knockdown, the sphere-forming ability of glioblastoma stem cells is reduced. Conversely, when MAD2L2 is overexpressed, the sphere-forming ability of glioblastoma stem cells is enhanced. F, G MAD2L2 knockdown downregulated the protein levels of stemness biomarkers, while MAD2L2 overexpression upregulated them. *P < 0.05, **P < 0.01, ***P < 0.001

Article Snippet: Diluted primary antibodies to CD133 (#64326S, Cell Signaling Technology), Nestin (#89529S, Cell Signaling Technology) and CD15 (#74180SF, Cell Signaling Technology) were added to the GSCs, followed by overnight incubation in a wet box at 4 °C.

Techniques: Expressing, Activation Assay, Immunofluorescence, Derivative Assay, Knockdown, Over Expression