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Image Search Results
Journal: JCI insight
Article Title: Glucose-dependent insulinotropic polypeptide receptor signaling alleviates gut inflammation in mice.
doi: 10.1172/jci.insight.174825
Figure Lengend Snippet: Figure 7: Gipr is predominantly expressed in non-immune cells within the lamina propria of the small bowel. (A) Relative Gipr expression across various tissues. (B) Relative radiant efficiency expression levels of tdTomato from GiprCre-Tdtomato+/+ mice normalized to the average radiant efficiency expression of tdTomatofl/fl across gut segments (n=3). (C) Gene expression relative to Rpl32 in manually dissected small bowel compartments (n=6). (D) mRNA expression relative to Rpl32 in lamina propria + muscle and epithelium throughout distinct segments of the small bowel (n=5-6). (E-F) Confocal microscopy of jejunum segments showing expression of GIPR-tdTomato (red), DAPI (blue) and (E) E-cadherin (CDH1) (green), or (F) CD45 (green) (40x magnification, scale bar: 20mm). (G) GIPR-tdTomato mean fluorescence intensity (MFI) of distinct cell populations isolated from the small bowel (n=4-8). (H) Gene expression in whole jejunum, LP + muscle, total cells post digestion (crude cells), and isolated CD146- and CD146+ cells via magnetic cell separation (n=5-11). Data are presented as Mean ± SD from samples from one experiment (A-F) and pooled from two independent experiments (G-H) with each data value corresponding to one mouse. ∗ P ≤ 0.05, ∗∗ P ≤ 0.01, ∗∗∗ P ≤ 0.001, ∗∗∗∗ P ≤ 0.0001 by one-way ANOVA followed by Tukey post-hoc tests. Abbreviations: BAT: brown adipose tissue; BS: brain
Article Snippet: 546 Magnetic Cell Separation: 547 Magnetic cell separation was performed using
Techniques: Expressing, Gene Expression, Confocal Microscopy, Fluorescence, Isolation, Magnetic Cell Separation
Journal: Frontiers in Cardiovascular Medicine
Article Title: A refined protocol for the isolation and monoculture of primary mouse renal peritubular endothelial cells
doi: 10.3389/fcvm.2023.1114726
Figure Lengend Snippet: Reagents and resources list for MRPEC isolation.
Article Snippet: CD146 (LSEC) antibody, PE, REAfinity , Recombinant human IgG1;
Techniques: Isolation, Immunofluorescence, Staining, Flow Cytometry, Recombinant, Concentration Assay, Control, Sterility, Membrane, Cell Culture, Centrifugation, Polymer, Blocking Assay, Purification, Binding Assay, Clinical Proteomics
Journal: Aging Cell
Article Title: Alterations of bovine nucleus pulposus cells with aging
doi: 10.1111/acel.13873
Figure Lengend Snippet: Antibodies used in flow cytometry analysis of bovine NP cells.
Article Snippet: Mouse anti‐human CD146 , AlexaFluor®647 , 5 ,
Techniques: Flow Cytometry, Purification