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Image Search Results
Journal: American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons
Article Title: Implanted adipose-derived stem cells attenuate small-for-size liver graft injury by secretion of VEGF in rats.
doi: 10.1111/j.1600-6143.2011.03870.x
Figure Lengend Snippet: Figure 1: Identification and purity of ADSCs. Flow cytometry revealed that the distribution of ADSCs that stained for CD14, CD31, CD34, CD45, CD68 and CD133 (shaded regions) did not differ from that of the isotype control (open regions). The majority of cells positively stained for CD29 and CD90 (shaded regions) compared with the isotype control cells (open regions) (A). Immunofluorescence analysis confirmed that ADSCs stained positively for CD29 and CD90, but not CD14, CD31, CD34, CD45, CD68 and CD133 (B). ADSCs resembled fibroblasts in culture and were successfully induced into adipocytes and osteocytes (C).
Article Snippet: Passage 3 cells were verified by immunostaining of surface markers for analysis by flow cytometry (Beckman Coulter, Brea, CA, USA) and immunofluorescence microscopy (Olympus, Tokyo, Japan) using fluorescent-conjugated antibodies against rat CD14, CD34, CD45, CD90 (Caltag Laboratories, San Francisco, CA, USA), CD29 (Biolegend, San Diego, CA, USA), CD31, CD68 (Abcam, Cambridge, MA, USA),
Techniques: Flow Cytometry, Staining, Control