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Image Search Results
Journal: bioRxiv
Article Title: CITEseq analysis of non-small-cell lung cancer lesions reveals an axis of immune cell activation associated with tumor antigen load and TP53 mutations
doi: 10.1101/2020.07.16.207605
Figure Lengend Snippet: A , Expression of key genes discriminating scRNAseq clusters of monocytes and MΦ, grouped by cell type annotation. Heatmap shows the number of UMI per cell. Clusters are shown using an even number of randomly selected cells from each, drawing from 35 tumor and 32 nLung samples. Cells were downsampled to 2000 UMI/cell. B , Scatter plots showing normalized CITEseq CD10 and CD206 surface marker counts on AMΦ, MoMΦ, and CD14+ monocytes in nLung of a representative patient. C , IHC of CD10 staining AMΦ in the airspaces of nLung tissue. D-G , Gene module analysis of monocyte and MΦ clusters. Correlation of gene module expression across all monocytes and MΦ ( D ). Module groups illustrate groups of correlated modules which are expressed most specifically on AMΦ, MoMΦ, and monocytes (see G ). Five example genes from each module, ranked by correlation to the other genes in the module and colored by total expression in monocytes and MΦ ( E ), boxplots showing Log 2 FC of module expression among all monocytes and MΦ between patient matched tumor and nLung samples ( F ), and normalized average cluster expression of modules ( G ). H , Log 2 FC and expression level of gene sets that are mutually exclusively expressed in CD14+ monocytes, AMΦ, and MoMΦ (See ).
Article Snippet: Primary antibodies were:
Techniques: Expressing, Marker, Staining
Journal: Endocrinology
Article Title: Terminating the stress: peripheral peptidolysis of proopiomelanocortin-derived regulatory hormones by the dermal microvascular endothelial cell extracellular peptidases neprilysin and angiotensin-converting enzyme.
doi: 10.1210/en.2006-1765
Figure Lengend Snippet: FIG. 1. NEP and ACE expression in microvascular ECs. Primary HDMEC (top) or cells of the EC line HMEC-1 (bottom) were stained with FITC-conjugated monoclonal Abs against human ACE (open histogram, bold line) or NEP (shaded histogram) and subjected to flow cytometry.
Article Snippet: For the analysis of HDMECs or HMEC-1 cell surface expression, detached cells were stained with a
Techniques: Expressing, Staining, Flow Cytometry