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ATCC
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Image Search Results
Journal: Asian Pacific Journal of Cancer Prevention : APJCP
Article Title: A Crosstalk between the Receptor Tyrosine Kinase-Like Orphan Receptors ROR1/2 and S1P Signaling Pathways in Lung Cancer
doi: 10.31557/APJCP.2024.25.3.725
Figure Lengend Snippet: ROR1 and ROR2 mRNA Expression in L132 Cells Treated with S1P. L132 cells were serum starved for 48 hrs and treated with vehicle or indicated S1P for 24 hrs. mRNA expression of ROR1 (A) and ROR2 (B) was quantified by qRT-PCR. GAPDH was used as a reference gene. Results represent an average of three experiments (N=3). Data is presented at mean ±SDEV. Means were compared by ANOVA followed by posthoc test. *p<0.01 compared to vehicle. C) ROR1 and ROR2 mRNA expression in A549 cells treated with PF543. Cells were treated with vehicle or 1µM concentration of PF543 for 24 hrs. mRNA expression of ROR1 and ROR2 was quantified by qRT-PCR.GAPDH was used as a housekeeping gene. Means were compared by t-test.*p<0.05 compared to vehicle;**p<0.01 compared to vehicle
Article Snippet: Establishment of cell lines and cell culture The lung cancer cell lines A549 and
Techniques: Expressing, Quantitative RT-PCR, Concentration Assay
Journal: Asian Pacific Journal of Cancer Prevention : APJCP
Article Title: A Crosstalk between the Receptor Tyrosine Kinase-Like Orphan Receptors ROR1/2 and S1P Signaling Pathways in Lung Cancer
doi: 10.31557/APJCP.2024.25.3.725
Figure Lengend Snippet: A-B) ROR1 and ROR2 mRNA Expression in SPHK1 knockdown BEAS2B. A) SPHK1 mRNA levels in HEK293T cells transfected with shSPHK1 when compared with shControl.B) Expression of ROR1 and ROR2 in SPHK1 knockdown BEAS2B. Fold Change expression was calculated. The level of significance was calculated using Two-way ANOVA (Sidak’s multiple comparison test). **P<0.01 compared to shControl. C-D) SPHK1 mRNA expression in ROR1 and ROR2 siRNA knocked down cells. L132 cells were transfected with ROR1 (C) and ROR2 (D), respectively. Forty-eight hours later, total RNA was isolated and mRNA expression of SPHK1 was quantified by qRT-PCR. GAPDH was used as a reference gene. Results represent an average of three experiments (N=3). Data is presented at the mean ±SDEV. **P<0.01 compared to untransfected
Article Snippet: Establishment of cell lines and cell culture The lung cancer cell lines A549 and
Techniques: Expressing, Knockdown, Transfection, Comparison, Isolation, Quantitative RT-PCR
Journal:
Article Title: Differential expression of immunoregulatory genes in monocytes in response to Porphyromonas gingivalis and Escherichia coli lipopolysaccharide
doi: 10.1111/j.1365-2249.2009.03920.x
Figure Lengend Snippet: Primers and probes for the quantification of gene expression using Taq man low-density arrays (TLDAs).
Article Snippet: RNA polymerase II (Hs00172187_ml) was used as an endogenous control. table ft1 table-wrap mode="anchored" t5 caption a7 Inflammatory mediator Primers and probe assay ID IL-1α (interleukin-1 alpha) Hs00174092_m1 IL-1β (interleukin-1 beta) Hs00174097_m1 IL-Ra (interleukin-1 receptor antagonist) Hs00277299_m1 IL-1R1 (interleukin-1 receptor-1) Hs00168392_m1 IL-1Acp (interleukin-1 accessory protein) Hs00370506_m1 ICE (IL-1 converting enzyme) (caspase-1) Hs00354836_m1 IL-18 (interleukin-18) Hs00155517_m1 IL-18R1 (interleukin-18 receptor) Hs00175381_m1 IL-18Acp (interleukin-1 accessory protein) Hs00187256_m1 IL-1F6 (interleukin-1 family 6) Hs00205367_m1 IL-1F7 (interleukin-1 family 7) Hs00367199_m1 IL-1F8 (interleukin-1 family 8) Hs00758166_m1 IL-1F9 (interleukin-1 family 9) Hs00219742_m1 IL-1F10 (interleukin-1 family 10) Hs00544661_m1 IL-1Rrp2 (IL-1 receptor-related protein 2) Hs00187259_m1 TNF-α (tumour necrosis factor alpha) Hs00174128_m1 TNFRSF1A (TNF alpha receptor superfamily 1A) Hs00236902_m1 TNFRSF1B (TNF alpha receptor superfamily 1B) Hs00153550_m1 TACE (TNF-α converting enzyme) Hs00234221_m1 IL-4 (interleukin-4) Hs00174122_m1 IL-6 (interleukin-6) Hs00174131_m1 OSM (oncostatin M) Hs00171165_m1 LIF (leukaemia inhibitory factor) Hs00171455_m1 OSMR (oncostatin M receptor) Hs00384278_m1 LIFR (leukaemia inhibitory factor receptor) Hs00158730_m1 IL-10 (interleukin 10) Hs00174086_m1 IL-12a (interleukin-12 chain a) Hs00168405_m1 IL-12b (interleukin-12 chain b) Hs00233688_m1 IL-32 (interleukin-32) Hs00170403_m1 GM-CSF (granulocyte macrophage-colony stimulating factor) Hs00171266_m1 IFN-γ (interferon gamma) Hs00174143_m1 CCL2 (monocyte chemottractant protein 1) Hs00234140_m1 CX3CL1 (fractalkine) Hs00171086_m1 CXCL5 (epithelial-derived neutrophil activating protein 78) (ENA-78) Hs00607029_g1 CCL5 (regulation on activation normal T cell expressed and secreted) (RANTES)
Techniques: Gene Expression, Activation Assay, Control
Journal:
Article Title: Differential expression of immunoregulatory genes in monocytes in response to Porphyromonas gingivalis and Escherichia coli lipopolysaccharide
doi: 10.1111/j.1365-2249.2009.03920.x
Figure Lengend Snippet: Escherichia coli lipopolysaccharide (LPS) upregulates differentially a subset of genes compared with Porphyromonas gingivalis LPS.
Article Snippet: RNA polymerase II (Hs00172187_ml) was used as an endogenous control. table ft1 table-wrap mode="anchored" t5 caption a7 Inflammatory mediator Primers and probe assay ID IL-1α (interleukin-1 alpha) Hs00174092_m1 IL-1β (interleukin-1 beta) Hs00174097_m1 IL-Ra (interleukin-1 receptor antagonist) Hs00277299_m1 IL-1R1 (interleukin-1 receptor-1) Hs00168392_m1 IL-1Acp (interleukin-1 accessory protein) Hs00370506_m1 ICE (IL-1 converting enzyme) (caspase-1) Hs00354836_m1 IL-18 (interleukin-18) Hs00155517_m1 IL-18R1 (interleukin-18 receptor) Hs00175381_m1 IL-18Acp (interleukin-1 accessory protein) Hs00187256_m1 IL-1F6 (interleukin-1 family 6) Hs00205367_m1 IL-1F7 (interleukin-1 family 7) Hs00367199_m1 IL-1F8 (interleukin-1 family 8) Hs00758166_m1 IL-1F9 (interleukin-1 family 9) Hs00219742_m1 IL-1F10 (interleukin-1 family 10) Hs00544661_m1 IL-1Rrp2 (IL-1 receptor-related protein 2) Hs00187259_m1 TNF-α (tumour necrosis factor alpha) Hs00174128_m1 TNFRSF1A (TNF alpha receptor superfamily 1A) Hs00236902_m1 TNFRSF1B (TNF alpha receptor superfamily 1B) Hs00153550_m1 TACE (TNF-α converting enzyme) Hs00234221_m1 IL-4 (interleukin-4) Hs00174122_m1 IL-6 (interleukin-6) Hs00174131_m1 OSM (oncostatin M) Hs00171165_m1 LIF (leukaemia inhibitory factor) Hs00171455_m1 OSMR (oncostatin M receptor) Hs00384278_m1 LIFR (leukaemia inhibitory factor receptor) Hs00158730_m1 IL-10 (interleukin 10) Hs00174086_m1 IL-12a (interleukin-12 chain a) Hs00168405_m1 IL-12b (interleukin-12 chain b) Hs00233688_m1 IL-32 (interleukin-32) Hs00170403_m1 GM-CSF (granulocyte macrophage-colony stimulating factor) Hs00171266_m1 IFN-γ (interferon gamma) Hs00174143_m1 CCL2 (monocyte chemottractant protein 1) Hs00234140_m1 CX3CL1 (fractalkine) Hs00171086_m1 CXCL5 (epithelial-derived neutrophil activating protein 78) (ENA-78) Hs00607029_g1 CCL5 (regulation on activation normal T cell expressed and secreted) (RANTES)
Techniques: