ccl5 Search Results


l132  (ATCC)
94
ATCC l132
ROR1 and ROR2 mRNA Expression in <t>L132</t> Cells Treated with S1P. L132 cells were serum starved for 48 hrs and treated with vehicle or indicated S1P for 24 hrs. mRNA expression of ROR1 (A) and ROR2 (B) was quantified by qRT-PCR. GAPDH was used as a reference gene. Results represent an average of three experiments (N=3). Data is presented at mean ±SDEV. Means were compared by ANOVA followed by posthoc test. *p<0.01 compared to vehicle. C) ROR1 and ROR2 mRNA expression in A549 cells treated with PF543. Cells were treated with vehicle or 1µM concentration of PF543 for 24 hrs. mRNA expression of ROR1 and ROR2 was quantified by qRT-PCR.GAPDH was used as a housekeeping gene. Means were compared by t-test.*p<0.05 compared to vehicle;**p<0.01 compared to vehicle
L132, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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86
Thermo Fisher snp ccl5 c 15874407 10
ROR1 and ROR2 mRNA Expression in <t>L132</t> Cells Treated with S1P. L132 cells were serum starved for 48 hrs and treated with vehicle or indicated S1P for 24 hrs. mRNA expression of ROR1 (A) and ROR2 (B) was quantified by qRT-PCR. GAPDH was used as a reference gene. Results represent an average of three experiments (N=3). Data is presented at mean ±SDEV. Means were compared by ANOVA followed by posthoc test. *p<0.01 compared to vehicle. C) ROR1 and ROR2 mRNA expression in A549 cells treated with PF543. Cells were treated with vehicle or 1µM concentration of PF543 for 24 hrs. mRNA expression of ROR1 and ROR2 was quantified by qRT-PCR.GAPDH was used as a housekeeping gene. Means were compared by t-test.*p<0.05 compared to vehicle;**p<0.01 compared to vehicle
Snp Ccl5 C 15874407 10, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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86
Thermo Fisher ccl5 mm01302427
ROR1 and ROR2 mRNA Expression in <t>L132</t> Cells Treated with S1P. L132 cells were serum starved for 48 hrs and treated with vehicle or indicated S1P for 24 hrs. mRNA expression of ROR1 (A) and ROR2 (B) was quantified by qRT-PCR. GAPDH was used as a reference gene. Results represent an average of three experiments (N=3). Data is presented at mean ±SDEV. Means were compared by ANOVA followed by posthoc test. *p<0.01 compared to vehicle. C) ROR1 and ROR2 mRNA expression in A549 cells treated with PF543. Cells were treated with vehicle or 1µM concentration of PF543 for 24 hrs. mRNA expression of ROR1 and ROR2 was quantified by qRT-PCR.GAPDH was used as a housekeeping gene. Means were compared by t-test.*p<0.05 compared to vehicle;**p<0.01 compared to vehicle
Ccl5 Mm01302427, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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99
Thermo Fisher gene exp ccl5 mm01302428 m1
ROR1 and ROR2 mRNA Expression in <t>L132</t> Cells Treated with S1P. L132 cells were serum starved for 48 hrs and treated with vehicle or indicated S1P for 24 hrs. mRNA expression of ROR1 (A) and ROR2 (B) was quantified by qRT-PCR. GAPDH was used as a reference gene. Results represent an average of three experiments (N=3). Data is presented at mean ±SDEV. Means were compared by ANOVA followed by posthoc test. *p<0.01 compared to vehicle. C) ROR1 and ROR2 mRNA expression in A549 cells treated with PF543. Cells were treated with vehicle or 1µM concentration of PF543 for 24 hrs. mRNA expression of ROR1 and ROR2 was quantified by qRT-PCR.GAPDH was used as a housekeeping gene. Means were compared by t-test.*p<0.05 compared to vehicle;**p<0.01 compared to vehicle
Gene Exp Ccl5 Mm01302428 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Thermo Fisher gene exp ccl5 hs00982282 m1
ROR1 and ROR2 mRNA Expression in <t>L132</t> Cells Treated with S1P. L132 cells were serum starved for 48 hrs and treated with vehicle or indicated S1P for 24 hrs. mRNA expression of ROR1 (A) and ROR2 (B) was quantified by qRT-PCR. GAPDH was used as a reference gene. Results represent an average of three experiments (N=3). Data is presented at mean ±SDEV. Means were compared by ANOVA followed by posthoc test. *p<0.01 compared to vehicle. C) ROR1 and ROR2 mRNA expression in A549 cells treated with PF543. Cells were treated with vehicle or 1µM concentration of PF543 for 24 hrs. mRNA expression of ROR1 and ROR2 was quantified by qRT-PCR.GAPDH was used as a housekeeping gene. Means were compared by t-test.*p<0.05 compared to vehicle;**p<0.01 compared to vehicle
Gene Exp Ccl5 Hs00982282 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Thermo Fisher gene exp ccl5 mm01302427 m1
ROR1 and ROR2 mRNA Expression in <t>L132</t> Cells Treated with S1P. L132 cells were serum starved for 48 hrs and treated with vehicle or indicated S1P for 24 hrs. mRNA expression of ROR1 (A) and ROR2 (B) was quantified by qRT-PCR. GAPDH was used as a reference gene. Results represent an average of three experiments (N=3). Data is presented at mean ±SDEV. Means were compared by ANOVA followed by posthoc test. *p<0.01 compared to vehicle. C) ROR1 and ROR2 mRNA expression in A549 cells treated with PF543. Cells were treated with vehicle or 1µM concentration of PF543 for 24 hrs. mRNA expression of ROR1 and ROR2 was quantified by qRT-PCR.GAPDH was used as a housekeeping gene. Means were compared by t-test.*p<0.05 compared to vehicle;**p<0.01 compared to vehicle
Gene Exp Ccl5 Mm01302427 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Thermo Fisher gene exp ccl5 hs00174575 m1
Primers and probes for the quantification of gene expression using Taq man low-density arrays (TLDAs).
Gene Exp Ccl5 Hs00174575 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Thermo Fisher gene exp ccl5 rn00579590 m1
Primers and probes for the quantification of gene expression using Taq man low-density arrays (TLDAs).
Gene Exp Ccl5 Rn00579590 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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94
Cell Signaling Technology Inc total 538 3646744 289 1 000
Primers and probes for the quantification of gene expression using Taq man low-density arrays (TLDAs).
Total 538 3646744 289 1 000, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


ROR1 and ROR2 mRNA Expression in L132 Cells Treated with S1P. L132 cells were serum starved for 48 hrs and treated with vehicle or indicated S1P for 24 hrs. mRNA expression of ROR1 (A) and ROR2 (B) was quantified by qRT-PCR. GAPDH was used as a reference gene. Results represent an average of three experiments (N=3). Data is presented at mean ±SDEV. Means were compared by ANOVA followed by posthoc test. *p<0.01 compared to vehicle. C) ROR1 and ROR2 mRNA expression in A549 cells treated with PF543. Cells were treated with vehicle or 1µM concentration of PF543 for 24 hrs. mRNA expression of ROR1 and ROR2 was quantified by qRT-PCR.GAPDH was used as a housekeeping gene. Means were compared by t-test.*p<0.05 compared to vehicle;**p<0.01 compared to vehicle

Journal: Asian Pacific Journal of Cancer Prevention : APJCP

Article Title: A Crosstalk between the Receptor Tyrosine Kinase-Like Orphan Receptors ROR1/2 and S1P Signaling Pathways in Lung Cancer

doi: 10.31557/APJCP.2024.25.3.725

Figure Lengend Snippet: ROR1 and ROR2 mRNA Expression in L132 Cells Treated with S1P. L132 cells were serum starved for 48 hrs and treated with vehicle or indicated S1P for 24 hrs. mRNA expression of ROR1 (A) and ROR2 (B) was quantified by qRT-PCR. GAPDH was used as a reference gene. Results represent an average of three experiments (N=3). Data is presented at mean ±SDEV. Means were compared by ANOVA followed by posthoc test. *p<0.01 compared to vehicle. C) ROR1 and ROR2 mRNA expression in A549 cells treated with PF543. Cells were treated with vehicle or 1µM concentration of PF543 for 24 hrs. mRNA expression of ROR1 and ROR2 was quantified by qRT-PCR.GAPDH was used as a housekeeping gene. Means were compared by t-test.*p<0.05 compared to vehicle;**p<0.01 compared to vehicle

Article Snippet: Establishment of cell lines and cell culture The lung cancer cell lines A549 and L132 were purchased from the National Center for Cell Science (NCCS), Pune India, while bronchial epithelial cells BEAS2B (ATCC# CRL-9609) was procured from the American Type Culture Collection (ATCC).

Techniques: Expressing, Quantitative RT-PCR, Concentration Assay

A-B) ROR1 and ROR2 mRNA Expression in SPHK1 knockdown BEAS2B. A) SPHK1 mRNA levels in HEK293T cells transfected with shSPHK1 when compared with shControl.B) Expression of ROR1 and ROR2 in SPHK1 knockdown BEAS2B. Fold Change expression was calculated. The level of significance was calculated using Two-way ANOVA (Sidak’s multiple comparison test). **P<0.01 compared to shControl. C-D) SPHK1 mRNA expression in ROR1 and ROR2 siRNA knocked down cells. L132 cells were transfected with ROR1 (C) and ROR2 (D), respectively. Forty-eight hours later, total RNA was isolated and mRNA expression of SPHK1 was quantified by qRT-PCR. GAPDH was used as a reference gene. Results represent an average of three experiments (N=3). Data is presented at the mean ±SDEV. **P<0.01 compared to untransfected

Journal: Asian Pacific Journal of Cancer Prevention : APJCP

Article Title: A Crosstalk between the Receptor Tyrosine Kinase-Like Orphan Receptors ROR1/2 and S1P Signaling Pathways in Lung Cancer

doi: 10.31557/APJCP.2024.25.3.725

Figure Lengend Snippet: A-B) ROR1 and ROR2 mRNA Expression in SPHK1 knockdown BEAS2B. A) SPHK1 mRNA levels in HEK293T cells transfected with shSPHK1 when compared with shControl.B) Expression of ROR1 and ROR2 in SPHK1 knockdown BEAS2B. Fold Change expression was calculated. The level of significance was calculated using Two-way ANOVA (Sidak’s multiple comparison test). **P<0.01 compared to shControl. C-D) SPHK1 mRNA expression in ROR1 and ROR2 siRNA knocked down cells. L132 cells were transfected with ROR1 (C) and ROR2 (D), respectively. Forty-eight hours later, total RNA was isolated and mRNA expression of SPHK1 was quantified by qRT-PCR. GAPDH was used as a reference gene. Results represent an average of three experiments (N=3). Data is presented at the mean ±SDEV. **P<0.01 compared to untransfected

Article Snippet: Establishment of cell lines and cell culture The lung cancer cell lines A549 and L132 were purchased from the National Center for Cell Science (NCCS), Pune India, while bronchial epithelial cells BEAS2B (ATCC# CRL-9609) was procured from the American Type Culture Collection (ATCC).

Techniques: Expressing, Knockdown, Transfection, Comparison, Isolation, Quantitative RT-PCR

Primers and probes for the quantification of gene expression using Taq man low-density arrays (TLDAs).

Journal:

Article Title: Differential expression of immunoregulatory genes in monocytes in response to Porphyromonas gingivalis and Escherichia coli lipopolysaccharide

doi: 10.1111/j.1365-2249.2009.03920.x

Figure Lengend Snippet: Primers and probes for the quantification of gene expression using Taq man low-density arrays (TLDAs).

Article Snippet: RNA polymerase II (Hs00172187_ml) was used as an endogenous control. table ft1 table-wrap mode="anchored" t5 caption a7 Inflammatory mediator Primers and probe assay ID IL-1α (interleukin-1 alpha) Hs00174092_m1 IL-1β (interleukin-1 beta) Hs00174097_m1 IL-Ra (interleukin-1 receptor antagonist) Hs00277299_m1 IL-1R1 (interleukin-1 receptor-1) Hs00168392_m1 IL-1Acp (interleukin-1 accessory protein) Hs00370506_m1 ICE (IL-1 converting enzyme) (caspase-1) Hs00354836_m1 IL-18 (interleukin-18) Hs00155517_m1 IL-18R1 (interleukin-18 receptor) Hs00175381_m1 IL-18Acp (interleukin-1 accessory protein) Hs00187256_m1 IL-1F6 (interleukin-1 family 6) Hs00205367_m1 IL-1F7 (interleukin-1 family 7) Hs00367199_m1 IL-1F8 (interleukin-1 family 8) Hs00758166_m1 IL-1F9 (interleukin-1 family 9) Hs00219742_m1 IL-1F10 (interleukin-1 family 10) Hs00544661_m1 IL-1Rrp2 (IL-1 receptor-related protein 2) Hs00187259_m1 TNF-α (tumour necrosis factor alpha) Hs00174128_m1 TNFRSF1A (TNF alpha receptor superfamily 1A) Hs00236902_m1 TNFRSF1B (TNF alpha receptor superfamily 1B) Hs00153550_m1 TACE (TNF-α converting enzyme) Hs00234221_m1 IL-4 (interleukin-4) Hs00174122_m1 IL-6 (interleukin-6) Hs00174131_m1 OSM (oncostatin M) Hs00171165_m1 LIF (leukaemia inhibitory factor) Hs00171455_m1 OSMR (oncostatin M receptor) Hs00384278_m1 LIFR (leukaemia inhibitory factor receptor) Hs00158730_m1 IL-10 (interleukin 10) Hs00174086_m1 IL-12a (interleukin-12 chain a) Hs00168405_m1 IL-12b (interleukin-12 chain b) Hs00233688_m1 IL-32 (interleukin-32) Hs00170403_m1 GM-CSF (granulocyte macrophage-colony stimulating factor) Hs00171266_m1 IFN-γ (interferon gamma) Hs00174143_m1 CCL2 (monocyte chemottractant protein 1) Hs00234140_m1 CX3CL1 (fractalkine) Hs00171086_m1 CXCL5 (epithelial-derived neutrophil activating protein 78) (ENA-78) Hs00607029_g1 CCL5 (regulation on activation normal T cell expressed and secreted) (RANTES) Hs00174575_m1 CXCL8 (IL-8) Hs00174103_m1 CXCL10 Hs00171042_m1 Leptin Hs00174877_m1 LeptinR (leptin receptor) Hs00174497_m1 ADIPOR1 (adiponectin receptor-1) Hs00360422_m1 ADIPOR2 (adiponectin receptor-2) Hs00226105_m1 Visfatin Hs00237184_m1 INSR (insulin receptor) Hs00169631_m1 RAGE (receptor for advanced glycation end products) Hs00153957_m1 CD14 Hs00169122_g1 Control genes GAPDH (glyceraldehyde-3-phosphate-dehydrogenase) Hs99999905_m1 18S Hs99999901_s1 RNA pol II Hs00222679_m1 Open in a separate window Primers and probes for the quantification of gene expression using Taq man low-density arrays (TLDAs).

Techniques: Gene Expression, Activation Assay, Control

Escherichia coli lipopolysaccharide (LPS) upregulates differentially a subset of genes compared with Porphyromonas gingivalis LPS.

Journal:

Article Title: Differential expression of immunoregulatory genes in monocytes in response to Porphyromonas gingivalis and Escherichia coli lipopolysaccharide

doi: 10.1111/j.1365-2249.2009.03920.x

Figure Lengend Snippet: Escherichia coli lipopolysaccharide (LPS) upregulates differentially a subset of genes compared with Porphyromonas gingivalis LPS.

Article Snippet: RNA polymerase II (Hs00172187_ml) was used as an endogenous control. table ft1 table-wrap mode="anchored" t5 caption a7 Inflammatory mediator Primers and probe assay ID IL-1α (interleukin-1 alpha) Hs00174092_m1 IL-1β (interleukin-1 beta) Hs00174097_m1 IL-Ra (interleukin-1 receptor antagonist) Hs00277299_m1 IL-1R1 (interleukin-1 receptor-1) Hs00168392_m1 IL-1Acp (interleukin-1 accessory protein) Hs00370506_m1 ICE (IL-1 converting enzyme) (caspase-1) Hs00354836_m1 IL-18 (interleukin-18) Hs00155517_m1 IL-18R1 (interleukin-18 receptor) Hs00175381_m1 IL-18Acp (interleukin-1 accessory protein) Hs00187256_m1 IL-1F6 (interleukin-1 family 6) Hs00205367_m1 IL-1F7 (interleukin-1 family 7) Hs00367199_m1 IL-1F8 (interleukin-1 family 8) Hs00758166_m1 IL-1F9 (interleukin-1 family 9) Hs00219742_m1 IL-1F10 (interleukin-1 family 10) Hs00544661_m1 IL-1Rrp2 (IL-1 receptor-related protein 2) Hs00187259_m1 TNF-α (tumour necrosis factor alpha) Hs00174128_m1 TNFRSF1A (TNF alpha receptor superfamily 1A) Hs00236902_m1 TNFRSF1B (TNF alpha receptor superfamily 1B) Hs00153550_m1 TACE (TNF-α converting enzyme) Hs00234221_m1 IL-4 (interleukin-4) Hs00174122_m1 IL-6 (interleukin-6) Hs00174131_m1 OSM (oncostatin M) Hs00171165_m1 LIF (leukaemia inhibitory factor) Hs00171455_m1 OSMR (oncostatin M receptor) Hs00384278_m1 LIFR (leukaemia inhibitory factor receptor) Hs00158730_m1 IL-10 (interleukin 10) Hs00174086_m1 IL-12a (interleukin-12 chain a) Hs00168405_m1 IL-12b (interleukin-12 chain b) Hs00233688_m1 IL-32 (interleukin-32) Hs00170403_m1 GM-CSF (granulocyte macrophage-colony stimulating factor) Hs00171266_m1 IFN-γ (interferon gamma) Hs00174143_m1 CCL2 (monocyte chemottractant protein 1) Hs00234140_m1 CX3CL1 (fractalkine) Hs00171086_m1 CXCL5 (epithelial-derived neutrophil activating protein 78) (ENA-78) Hs00607029_g1 CCL5 (regulation on activation normal T cell expressed and secreted) (RANTES) Hs00174575_m1 CXCL8 (IL-8) Hs00174103_m1 CXCL10 Hs00171042_m1 Leptin Hs00174877_m1 LeptinR (leptin receptor) Hs00174497_m1 ADIPOR1 (adiponectin receptor-1) Hs00360422_m1 ADIPOR2 (adiponectin receptor-2) Hs00226105_m1 Visfatin Hs00237184_m1 INSR (insulin receptor) Hs00169631_m1 RAGE (receptor for advanced glycation end products) Hs00153957_m1 CD14 Hs00169122_g1 Control genes GAPDH (glyceraldehyde-3-phosphate-dehydrogenase) Hs99999905_m1 18S Hs99999901_s1 RNA pol II Hs00222679_m1 Open in a separate window Primers and probes for the quantification of gene expression using Taq man low-density arrays (TLDAs).

Techniques: