Journal: Virus Research

Article Title: Modulatory effects of platelet-rich plasma on viral kinetics of BoAHV-1.1, BoGHV-4, and BVDV in bovine cell cultures: A proof-of-concept study

doi: 10.1016/j.virusres.2025.199653

Figure Lengend Snippet: Extracellular and intracellular BoAHV-1.1 titers (log₁₀ TCID₅₀/ml) at 24, 48, and 72 h post-infection (hpi) in MDBK and BESc cultures. Panel A: extracellular titers in MDBK cells; Panel B: intracellular titers in MDBK cells; Panel C: extracellular titers in BESc; Panel D: intracellular titers in BESc.Values represent the means ± SD of three independent replicates. Asterisks (*) indicate significant differences (p < 0.01) between PRP (5 % or 10 %) and FBS (10 %) at each time point.

Article Snippet: Madin-Darby bovine kidney (MDBK) cells from the American Type Culture Collection (ATCC, Rockville, MD, USA) were used for in vitro assays.

Techniques: Infection

Journal: Virus Research

Article Title: Modulatory effects of platelet-rich plasma on viral kinetics of BoAHV-1.1, BoGHV-4, and BVDV in bovine cell cultures: A proof-of-concept study

doi: 10.1016/j.virusres.2025.199653

Figure Lengend Snippet: Cytopathic effect (CPE) of BoAHV-1.1 observed at 48 h post-infection (hpi) under different culture conditions in MDBK and BESc cells. Panels A–D correspond to MDBK cultures: A, uninfected control; B, 5 % PRP; C, 10 % PRP; D, 10 % FBS. Panels E–H correspond to BESc cultures: E, uninfected control; F, 5 % PRP; G, 10 % PRP; H, 10 % FBS. Images were captured at 10× magnification. The cytopathic effect is characterized by cell rounding and detachment, with more pronounced degeneration observed in FBS-treated groups compared with PRP-treated or control cultures.

Article Snippet: Madin-Darby bovine kidney (MDBK) cells from the American Type Culture Collection (ATCC, Rockville, MD, USA) were used for in vitro assays.

Techniques: Infection, Control

Journal: Virus Research

Article Title: Modulatory effects of platelet-rich plasma on viral kinetics of BoAHV-1.1, BoGHV-4, and BVDV in bovine cell cultures: A proof-of-concept study

doi: 10.1016/j.virusres.2025.199653

Figure Lengend Snippet: . Extracellular and intracellular BoGHV-4 titers (log₁₀ TCID₅₀/ml) at 24, 48, and 72 h post-infection (hpi) in MDBK and BESc cultures. Panel A: extracellular titers in MDBK cells; Panel B: intracellular titers in MDBK cells; Panel C: extracellular titers in BESc; Panel D: intracellular titers in BESc. Values represent the means ± SD of three independent replicates. Asterisks (*) indicate significant differences (p < 0.01 for MDBK; p < 0.05 for BESc) between PRP (5 % or 10 %) and FBS at each time point.

Article Snippet: Madin-Darby bovine kidney (MDBK) cells from the American Type Culture Collection (ATCC, Rockville, MD, USA) were used for in vitro assays.

Techniques: Infection

Journal: Virus Research

Article Title: Modulatory effects of platelet-rich plasma on viral kinetics of BoAHV-1.1, BoGHV-4, and BVDV in bovine cell cultures: A proof-of-concept study

doi: 10.1016/j.virusres.2025.199653

Figure Lengend Snippet: Cytopathic effect (CPE) of BoGHV-4 (strain 07/435) observed at 48 h post-infection (hpi) under different culture conditions in MDBK and BESc cells. Panels A–D correspond to MDBK cultures: A, uninfected control; B, 5 % PRP; C, 10 % PRP; D, 10 % FBS. Panels E–H correspond to BESc cultures: E, uninfected control; F, 5 % PRP; G, 10 % PRP; H, 10 % FBS. Images were captured at 40× magnification. The cytopathic effect is characterized by cell rounding and detachment, with more pronounced alterations observed in the 10 % FBS condition compared with PRP-treated and control groups.

Article Snippet: Madin-Darby bovine kidney (MDBK) cells from the American Type Culture Collection (ATCC, Rockville, MD, USA) were used for in vitro assays.

Techniques: Infection, Control

Journal: Virus Research

Article Title: Modulatory effects of platelet-rich plasma on viral kinetics of BoAHV-1.1, BoGHV-4, and BVDV in bovine cell cultures: A proof-of-concept study

doi: 10.1016/j.virusres.2025.199653

Figure Lengend Snippet: Extracellular and intracellular BVDV titers (log₁₀ TCID₅₀/ml) at 24, 48, and 72 h post-infection (hpi) in MDBK and BESc cultures. Panel A: extracellular titers in MDBK cells; Panel B: intracellular titers in MDBK cells; Panel C: extracellular titers in BESc; Panel D: intracellular titers in BESc. Values represent the means ± SEM of three independent replicates. Asterisks (*) indicate significant differences (p < 0.01) between PRP (5 % or 10 %) and FBS at each time point.

Article Snippet: Madin-Darby bovine kidney (MDBK) cells from the American Type Culture Collection (ATCC, Rockville, MD, USA) were used for in vitro assays.

Techniques: Infection

Journal: Virus Research

Article Title: Modulatory effects of platelet-rich plasma on viral kinetics of BoAHV-1.1, BoGHV-4, and BVDV in bovine cell cultures: A proof-of-concept study

doi: 10.1016/j.virusres.2025.199653

Figure Lengend Snippet: Cytopathic effect (CPE) of Bovine Viral Diarrhea Virus (BVDV, strain NADL) observed at 48 h post-infection (hpi) under different culture conditions in MDBK and BESc cells. Panels A–D correspond to MDBK cultures: A, uninfected control; B, 5 % PRP; C, 10 % PRP; D, 10 % FBS. Panels E–H correspond to BESc cultures: E, uninfected control; F, 5 % PRP; G, 10 % PRP; H, 10 % FBS. Images were captured at 40× magnification. The cytopathic effect is characterized by cell rounding and detachment, with more severe degeneration observed in the 10 % FBS condition compared with PRP-treated and control groups.

Article Snippet: Madin-Darby bovine kidney (MDBK) cells from the American Type Culture Collection (ATCC, Rockville, MD, USA) were used for in vitro assays.

Techniques: Virus, Infection, Control

Journal: Cell Biology and Toxicology

Article Title: SENP3 mediated DeSUMOylation of macrophage derived CCL17 accelerates atherosclerosis via regulation of Treg

doi: 10.1007/s10565-025-10099-3

Figure Lengend Snippet: Macrophages stimulated by oxLDL affect the chemotaxis of Treg through CCL17 and CCL22 competition. Macrophages were stimulated with oxLDL, and Treg cells were co-cultured with macrophage supernatant. A Immunofluorescence detection of co-localization of CCL22 and macrophages (CD68) in plaque tissue of AS mice. B The content of CCL22 in the supernatant of macrophages was detected by ELISA. C Transwell detected the effect of macrophages on the chemotaxis of Treg. ( D - E ) Transwell detected the migration of Treg cells. * p < 0.05, ** p < 0.01, *** p < 0.001. N = 3

Article Snippet: Prior to the transwell migration assay, the induced Tregs were treated with recombinant mouse CCL17 (MCE, HY-P71891A) or recombinant mouse CCL22 (MCE, HY-P7248) or anti-CCL17 (R&D, Catalog #: MAB529) and anti-CCL22 antibodies (R&D, Catalog #: MAB529).

Techniques: Chemotaxis Assay, Cell Culture, Immunofluorescence, Enzyme-linked Immunosorbent Assay, Migration

Journal: PLoS ONE

Article Title: LncRNA HOTAIR promotes proliferation, invasion and migration in NSCLC cells via the CCL22 signaling pathway

doi: 10.1371/journal.pone.0263997

Figure Lengend Snippet: A, B: the expression of HOTAIR and CCL22 mRNA were detected by RT-qPCR. C: CCL22 protein analyzed by ELISA assay. ****, P<0.001.

Article Snippet: The concentration of CCL22 in the supernatant was tested according to the instructions of ELISA kit operation (CUSABIO, CSB-E04660h).

Techniques: Expressing, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay

Journal: PLoS ONE

Article Title: LncRNA HOTAIR promotes proliferation, invasion and migration in NSCLC cells via the CCL22 signaling pathway

doi: 10.1371/journal.pone.0263997

Figure Lengend Snippet: A: The HOTAIR and CCL22 mRNA expression examined by RT-qPCR B: Cell apoptosis detected by Flow cytometry. C: The cell proliferation tested by MTS assay. D: The CCL22 protein expression tested by ELISA. E: The cell migration and invasion detected by Transwell assay. **, P<0.05; ***, P<0.01; ****, P<0.001;ns, no significance.

Article Snippet: The concentration of CCL22 in the supernatant was tested according to the instructions of ELISA kit operation (CUSABIO, CSB-E04660h).

Techniques: Expressing, Quantitative RT-PCR, Flow Cytometry, MTS Assay, Enzyme-linked Immunosorbent Assay, Migration, Transwell Assay

Journal: European Journal of Nuclear Medicine and Molecular Imaging

Article Title: ApoSense : a novel technology for functional molecular imaging of cell death in models of acute renal tubular necrosis

doi: 10.1007/s00259-005-1905-x

Figure Lengend Snippet: Concomitant confocal imaging of HeLa cell undergoing apoptosis by DDC (green fluorescence) and annexin V (red fluorescence). DDC accumulates within the cytoplasm of the apoptotic cell while annexin V is attached to the external membrane

Article Snippet: Human adult T-cell leukemia Jurkat cells (clone E6-1) and cervical carcinoma HeLa cells (CCL-2.2), were purchased from ATCC (Rockville, MD, USA) and grown in Dulbecco’s modified Eagle’s medium with 10% fetal calf serum at 37°C in 5% CO 2 .

Techniques: Imaging, Fluorescence, Membrane