Journal: The Journal of Clinical Investigation

Article Title: TRAIL agonists rescue mice from radiation-induced lung, skin, or esophageal injury

doi: 10.1172/JCI173649

Figure Lengend Snippet: ( A ) Experimental timeline. Female C57BL/6 mice orthotopically injected with e0771 on day 0 and when tumors reached 2–5 mm in size mice were irradiated with a whole-thorax irradiation dose of 20 Gy and treated with either TLY012, ONC201, or the combination ( n = 3–4 per treatment per group). (Created in BioRender. Strandberg J. 2024. https://BioRender.com/c65v064. ) ( B and C ) Tumors were removed after mice were euthanized 18 days after cell injection, and weight and volume were calculated. ( D ) Pulse oximetry readings before radiation and 9 days after radiation showed oxygen saturation more conserved in the TLY012-treated group compared with irradiated controls. Values are mean ± SEM. ( E ) Statistical analysis of cytokine fold change showed significant decrease in levels of MDC/CCL22 ( P = 0.035) in comparison with irradiated controls (1-way ANOVA with Tukey’s post hoc test).

Article Snippet: Seven-week-old Trail –/– female mice received a single thoracic x-ray irradiation dose of 20 Gy with shielding of other organs and were treated with either 20 μg of anti-CCL22 antibody (AF439, R&D Systems Inc.) dissolved in 500 μL of sterile normal saline via i.p. injection every other day for 2 weeks or 20 μg of polyclonal goat IgG (AB-108-C, R&D Systems Inc.) dissolved in 500 μL of normal saline via i.p. injection every other day for 2 weeks ( n = 5 per treatment per group).

Techniques: Injection, Irradiation, Comparison

Journal: The Journal of Clinical Investigation

Article Title: TRAIL agonists rescue mice from radiation-induced lung, skin, or esophageal injury

doi: 10.1172/JCI173649

Figure Lengend Snippet: ( A ) Experimental timeline of Trail –/– female mice treated with 20 μg of anti-CCL22 in 500 μL of saline or 20 μg of goat IgG in 500 μL of saline every other day for 2 weeks ( n = 5 per treatment per group). (Created in BioRender. Strandberg J. 2024. https://BioRender.com/j52q830. ) ( B ) H&E images of each mouse 2 weeks after thoracic irradiation (original magnification, ×20). Scale bar: 100 μm. ( C ) Quantification of inflammatory scores provided by a blinded pathologist showed decrease in inflammation in Trail –/– female mice treated with anti-CCL22 ( n = 5 per treatment per group) but not to a significant extent (1-tailed Mann-Whitney test) ( P = 0.1032). Values are mean ± SEM.

Article Snippet: Seven-week-old Trail –/– female mice received a single thoracic x-ray irradiation dose of 20 Gy with shielding of other organs and were treated with either 20 μg of anti-CCL22 antibody (AF439, R&D Systems Inc.) dissolved in 500 μL of sterile normal saline via i.p. injection every other day for 2 weeks or 20 μg of polyclonal goat IgG (AB-108-C, R&D Systems Inc.) dissolved in 500 μL of normal saline via i.p. injection every other day for 2 weeks ( n = 5 per treatment per group).

Techniques: Saline, Irradiation, MANN-WHITNEY

Journal: Antibiotics

Article Title: Biological Activities of Essential Oils from Leaves of Paramignya trimera (Oliv.) Guillaum and Limnocitrus littoralis (Miq.) Swingle

doi: 10.3390/antibiotics9040207

Figure Lengend Snippet: Cytotoxicity and antiviral activity of essential oils from P. trimera and L. littoralis against representatives of ssRNA + (HIV-1, YFV, BVDV, Sb-1, CV- B4), ssRNA − (RSV, VSV), dsRNA (Reo-1), and dsDNA (HSV-1, VV) viruses.

Article Snippet: MT-4 cells (CD4+ human T cells containing an integrated HTLV-1 genome) were cultured in Roswell Park Memorial Institute 1640 (RPMI-1640) medium supplemented with 10% fetal bovine serum (FBS), 100 units/mL penicillin G, and 100 μg/mL streptomycin; Madin Darby Bovine Kidneys (MDBK) (ATCC CCL 22 (NBL-1) Bos taurus) were cultured in Minimum Essential Medium with Earle’s salts (MEM-E), L-glutamine, 1mM sodium pyruvate and 25mg/L kanamycin, supplemented with 10% horse serum; Baby Hamster Kidneys (BHK-21) (ATCC CCL 10 (C-13) Mesocricetus auratus) were cultured in (MEM-E), L-glutamine, 1mM sodium pyruvate and 25 mg/L kanamycin, supplemented with 10% FBS; and Monkey kidneys (Vero-76) (ATCC CRL 1587 Cercopithecus Aethiops) were cultured in in Dulbecco’s Modified Eagle Medium (D-MEM) with L-glutamine and 25 mg/L kanamycin, supplemented with 10% FBS.

Techniques: Activity Assay, Virus