Journal: International journal of cardiology. Cardiovascular risk and prevention

Article Title: Chemokine ligand 18 predicts all-cause mortality in patients hospitalized with chest pain of suspected coronary origin.

doi: 10.1016/j.ijcrp.2024.200264

Figure Lengend Snippet: Fig. 2. Receiver operated characteristic (ROC) curves depicting the relationship between CCL18, hsCRP and BNP, respectively, and all-cause mortality in the total population at 7-years follow-up.

Article Snippet: CCL18 (ng/mL) (Cat#DY394) was analysed by enzyme-linked immunosorbent assay (ELISA) in duplicate in plasma from 849 patients using antibodies from R&D Systems (Minneapolis, MN, USA), in a 384-well format using a combination of a SELMA (Jena, Germany) pipetting robot and a BioTek (Winooski, VT, USA) dispenser/washer.

Techniques:

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: CC chemokine ligand 18, an atopic dermatitis-associated and dendritic cell-derived chemokine, is regulated by staphylococcal products and allergen exposure.

doi: 10.4049/jimmunol.173.9.5810

Figure Lengend Snippet: FIGURE 1. CCL18 expression is specifically associated with an atopic dermatitis phenotype. a–d, Quantitative real-time PCR analyses of CCL18, CCL17, CCL20, and CXCL8 in normal vs inflamed skin. Values are expressed in femtograms of the target gene in 25 ng of total cDNA. Measurements of individual samples and mean SD are shown. A Student’s t test was performed; , p 0.05; , p 0.01.

Article Snippet: Dilution series of recombinant human CCL18 protein were used for standard curves (R&D Systems).

Techniques: Expressing, Real-time Polymerase Chain Reaction

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: CC chemokine ligand 18, an atopic dermatitis-associated and dendritic cell-derived chemokine, is regulated by staphylococcal products and allergen exposure.

doi: 10.4049/jimmunol.173.9.5810

Figure Lengend Snippet: FIGURE 2. Dermal DCs and keratinocytes are the cellular origin of CCL18 expression in atopic skin inflammation. CCL18 expression in nor- mal, nonlesional atopic, and lesional atopic skin. a, Absence of CCL18 in the normal skin. b, Absence of CCL18 in nonlesional atopic skin. c and d, Marked expression in inflamed atopic skin and marked expression on cells with dendritic morphology (d, inset). e, CCL18-positive cells clustered in perivascular pockets. f and g, CCL18-positive cells in the epidermis ex- hibiting a dendritic morphology. h, CCL18-positive staining in the upper- most part of the epidermis. Representative results of 1 of 10 patients are shown. Magnifications were as follows: 250 (a–c), 400 (d–f), and 800 (g and h).

Article Snippet: Dilution series of recombinant human CCL18 protein were used for standard curves (R&D Systems).

Techniques: Expressing, Staining

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: CC chemokine ligand 18, an atopic dermatitis-associated and dendritic cell-derived chemokine, is regulated by staphylococcal products and allergen exposure.

doi: 10.4049/jimmunol.173.9.5810

Figure Lengend Snippet: FIGURE 3. T cell effector cytokines and staphylococcal products regulate CCL18. Quantitative real-time PCR and ELISA analyses of CCL18 expression in cellular constituents of the skin. a, Analysis of CCL18 in cDNA obtained from cultured human primary keratinocytes, dermal fibroblasts, dermal microvascular endothelial cells treated with medium alone, or with TNF-/IL-1, IFN-, or IL-4 for 18 h. Values are expressed in femtograms of the target gene in 25 ng of total cDNA. Representative data from single donors. b and c, Analysis of CCL18 mRNA in monocytes, or monocyte-derived dendritic cell subsets and Langerhans cells 6 and 24 h after stimulation with LPS, LTA, PGN, mannan, SEB, and CD40L. Values are expressed in picograms of the target gene in 25 ng of total cDNA. d, ELISA analysis of CCL18 in supernatants obtained from cultured monocytes, DCs, and Langerhans cells treated with medium alone or with LPS, LTA, PGN, mannan, SEB, and CD40L. Values are expressed in nanograms of CCL18 protein produced by 106 cells.

Article Snippet: Dilution series of recombinant human CCL18 protein were used for standard curves (R&D Systems).

Techniques: Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Expressing, Cell Culture, Derivative Assay, Produced

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: CC chemokine ligand 18, an atopic dermatitis-associated and dendritic cell-derived chemokine, is regulated by staphylococcal products and allergen exposure.

doi: 10.4049/jimmunol.173.9.5810

Figure Lengend Snippet: FIGURE 4. Allergen and superantigen exposure induce the expression of CCL18 in vitro and in vivo. a, Quantitative real-time PCR analysis of CCL18 in atopic dermatitis patients (n 8) subjected to atopy patch tests with house dust mite allergen. Skin samples were obtained 0, 2, 6, and 48 h after allergen exposure. Values are expressed as femtograms of the target gene in 25 ng of total cDNA. A Student’s t test was performed, , p 0.05. b and c, CCL18 expression before (b) and 24 h after (c) topical exposure of atopic dermatitis patients with house dust mite allergens; magnification, 250. d, Quantitative real-time PCR analysis of CCL18 expression in single cell suspensions of lesional atopic dermatitis skin 24 h after in vitro stimulation with the house dust mite Ag Der p 2. e, Quantitative real-time PCR analysis of CCL18 in atopic dermatitis patients (n 6) before and 2, 6, and 48 h after SEB exposure. f, Analysis of CCL18 expression in PBMCs 6 and 24 h after treatment with SEB. Values are expressed as femtograms of target gene in 25 ng of total cDNA. A Student’s t test was performed; , p 0.05.

Article Snippet: Dilution series of recombinant human CCL18 protein were used for standard curves (R&D Systems).

Techniques: Expressing, In Vitro, In Vivo, Real-time Polymerase Chain Reaction

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: CC chemokine ligand 18, an atopic dermatitis-associated and dendritic cell-derived chemokine, is regulated by staphylococcal products and allergen exposure.

doi: 10.4049/jimmunol.173.9.5810

Figure Lengend Snippet: FIGURE 5. Increased CCL18 expression in hapten-induced allergic but not in chemical induced irritant skin inflammation. a–d, The detection of CCL18 expression was performed by immunohistochemistry in the skin of nontreated as well as NiSO4- or SLS-treated Ni-sensitized individuals. a and b, CCL18 expression is not detected before exposure of Ni-sensitized patients to NiSO4 (a). However, an increased expression of CCL18 is ob- served 48 h after topical application of NiSO4 (b). c and d, CCL18 ex- pression before (c) and 48 h after (d) exposure of Ni-sensitized patients to SLS. Representative results of one of seven patients are shown. Magnifi- cation, 400. e, Quantitative real-time PCR analysis of CCL18 expression in Ni-sensitive individuals (n 7) subjected to NiSO4 or SLS patch test. Skin samples were obtained 0, 2, 6, and 48 h after exposure to NiSO4 or SLS. Values are expressed as femtograms of target gene in 25 ng of total cDNA. A Student’s t test was performed; , p 0.05.

Article Snippet: Dilution series of recombinant human CCL18 protein were used for standard curves (R&D Systems).

Techniques: Expressing, Immunohistochemistry, Real-time Polymerase Chain Reaction

Journal: Arthritis & Rheumatology (Hoboken, N.j.)

Article Title: Antifibrotic effects of specific targeting of the 5‐hydroxytryptamine 2B receptor (5‐HT 2B R) in murine models and ex vivo models of scleroderma skin

doi: 10.1002/art.43151

Figure Lengend Snippet: Inhibition of 5‐HT 2B R by AM1476 modulates the expression of MAOB, SCL6A4, CCL2, CCL5, PAI‐1, and P‐STAT3 in murine cGvHD and PCS slices of SSc skin. (A and B) Murine cGvHD model. (A) Representative images of immunofluorescence staining and (B) quantification of Maob, Slc6a4, Ccl2, CCl5, Pai‐1, and P‐Stat3 in the skin of syngeneically and allogeneically transplanted mice with or without AM1476 treatment. All data are presented as mean ± SEM, with individual values displayed as column plus dots. Differences between the groups were tested for their statistical significance by one‐way analysis of variance with Dunnett's multiple comparison. Adjusted P values less than 0.05 were considered significant. Adjusted P values are expressed as follows: *0.05 > P > 0.01; **0.01 > P > 0.001; ***0.001 > P > 0.0001; **** P < 0.0001 as compared to the control allogenic group. (C and D) PCS slices of SSc skin. (C) Representative immunofluorescence staining images and (D) quantification of MAOB, SLC6A4, CCL2, CCL18, PAI‐1, and P‐STAT3 in SSc‐PCS with or without AM1476. All data points are presented as individual values displayed as dots. Differences between the groups were tested for their statistical significance by two‐way analysis of variance with Sidak's multiple comparison. Adjusted P values less than 0.05 were considered significant. Adjusted P values are expressed as follows: *0.05 > P > 0.01; **0.01 > P > 0.001; ***0.001 > P > 0.0001; **** P < 0.0001 as compared to the SSc‐PCS control group. 5‐HT 2B R, 5‐hydroxytryptamine 2B receptor; cGvHD, chronic graft‐versus‐host disease; PCS, precision cut skin; SSc, systemic sclerosis. Color figure can be viewed in the online issue, which is available at http://onlinelibrary.wiley.com/doi/10.1002/art.43151/abstract .

Article Snippet: The staining of skin sections was performed by using the antibodies against CCL2 (LS‐C169178‐100, LSBio, 1:100 dilution), CCL5 (MAB478‐100, Biotechne, 1:100 dilution), CCL18 (22303‐1‐AP, Proteintech, 1:100 dilution), PAI‐1 (66261‐1‐Ig, Proteintech, 1:100 dilution), P‐STAT3 (MA5‐15193, Thermo Scientific, 1:100 dilution), MAOB (12602‐1‐AP, Proteintech, 1:100 dilution), or SLC6A4 (LS‐C154958‐100, LSBio, 1:100 dilution).

Techniques: Inhibition, Expressing, Immunofluorescence, Staining, Comparison, Control