Journal: Genome Medicine

Article Title: A case study of an integrative genomic and experimental therapeutic approach for rare tumors: identification of vulnerabilities in a pediatric poorly differentiated carcinoma

doi: 10.1186/s13073-016-0366-0

Figure Lengend Snippet: a Sensitivity of PDX tumors to the mTOR inhibitor, temsirolimus. Chemoresistance to carboplatin and JQ1 were observed following a transient period of response. Mean and standard error of the mean (SEM) are shown. b Phosphorylation level of RPS6 upon temsirolimus treatment. c C-MYC and N-MYC protein levels upon JQ1 treatment. d Temsirolimus treatment results in decreased Ki-67 staining with concomitant increase in cleaved caspase 3 (Cl. CASP 3) following short-term (3 days) and long-term (50 days) treatments. * p < 0.05, ** p < 0.01. e Tumor growth after temsirolimus treatment withdrawal. Mean and SEM are shown. f Temsirolimus treatment can successfully rescue and induce tumor regression in carboplatin-resistant tumors. Mean and SEM are shown. g Combination therapy (temsirolimus and irinotecan) does not result in increased anti-tumor activity. Tumor regrowth is observed with withdrawal of treatment. Mean and SEM are shown

Article Snippet: Selumetinib, carboplatin, irinotecan, and temsirolimus were purchased from MedChem Express.

Techniques: Phospho-proteomics, Staining, Activity Assay

Journal: Diabetes

Article Title: The Role of TRAPγ/SSR3 in Preproinsulin Translocation Into the Endoplasmic Reticulum.

doi: 10.2337/db21-0638

Figure Lengend Snippet: Figure 1—The TRAPg/SSR3 subunit of the TRAP/SSR complex contributes to the efficiency of recombinant proinsulin biosynthesis. A: Schematic of the TRAP/SSR complex highlights the predominant cytosolic exposure of the TRAPg/SSR3 subunit, including a hypothetical contact with a signal peptide N-terminus during polypeptide translocation (red line). B: Control (Ctrl) (wild-type [WT] 293T cells) and SSR3- KO 293T cells were transfected with untagged human preproinsulin plasmids. At 48 h posttransfection, cell lysates were analyzed by reducing SDS-PAGE gel and immunoblotting with antiproinsulin and anti-SSR1–4 antibodies as indicated. C: Quantitation (mean ± SD) of preproinsulin, proinsulin, and TRAP/SSR subunit protein levels from four independent experiments like that shown in B (normalized to tubulin). *P < 0.05 compared with Ctrl. D: Ctrl and SSR3-KO 293T cells were transfected with human preproinsulin plasmids. At 48 h post- transfection, the cells were pulse labeled with 35S-Met/Cys for the times indicated; this is a representative pulse labeling from three identi- cal experiments. Cell lysates (normalized to trichloroacetic acid–precipitable counts) were subjected to immunoprecipitation with anti- insulin and analyzed by reducing SDS-PAGE and phosphorimaging; both the absolute and relative amounts of recovered preproinsulin and proinsulin are shown in the graph.

Article Snippet: Mouse anti-rat proinsulin (CCI-17) and rabbit polyclonal anti-SSR1 antibodies (NB100-73013 and NBP1-86912, respectively) were from Novus Biologicals (Littleton, CO).

Techniques: Recombinant, Translocation Assay, Control, Transfection, SDS Page, Western Blot, Quantitation Assay, Labeling, Immunoprecipitation

Journal: Frontiers in endocrinology

Article Title: Pharmacological Inhibition of Inositol-Requiring Enzyme 1α RNase Activity Protects Pancreatic Beta Cell and Improves Diabetic Condition in Insulin Mutation-Induced Diabetes.

doi: 10.3389/fendo.2021.749879

Figure Lengend Snippet: FIGURE 5 | STF improves Akita islet b-cell function. (A) Glucose-stimulated insulin secretion of 20 islets isolated from Akita mice treated with STF or vehicle and incubated with 2.5 mM and 16.7 mM glucose. Secreted insulin was measured by ELISA. The data was presented as fold change and normalized with total protein concentration, with the amount of insulin secreted in response to 2.5 mM glucose from vehicle-treated group set to 1.0. (B–E) mRNA levels for indicated genes were analyzed in islets isolated from Akita mice treated with STF or vehicle by qRT-PCR. The results are expressed as fold change and are representative of 3 independent experiments. (F) Proinsulin content measurement by ELISA as detailed in Methods and Materials. (G, H) Proinsulin content and secretion measurement. 20 islets isolated from Akita mice treated with STF or vehicle were incubated with 2.5 mM and 16.7 mM glucose. Secreted proinsulin was measured by ELISA. Proinsulin content measurement by ELISA as detailed in Methods and Materials. The data was presented as fold change and normalized with total protein concentration, with the amount of proinsulin in response to 2.5 mM glucose from vehicle-treated group set to 1.0. (I) Proinsulin misfolding detection by Western blotting under nonreducing condition. Islets were treated with compounds at indicated concentrations for 16 hours. The data is representative of 3 independent experiments. (J–L) Ultrastructure of b-cells in islets isolated from Akita treated with STF as in by transmission electron microscopy. Images at the top panel and the bottom panel were taken at 3,000x and 10,000x, respectively. Arrows point to dark mature insulin granules. *P < 0.05, **P < 0.01, and ***P < 0.001.

Article Snippet: The nonreducing gels were incubated in 25 mM dithiothreitol (DTT) solution for 10 min at room temperature before being transferred to PVDFmembranes for 25 min at 4°C at 40 V. Membranes were probed with anti-proinsulin antibody (CCI-17, NOVUS) and HRP-conjugated secondary antibodies (1:3000; Santa Cruz Biotechnology, CA, USA).

Techniques: Cell Function Assay, Isolation, Incubation, Enzyme-linked Immunosorbent Assay, Protein Concentration, Quantitative RT-PCR, Western Blot, Transmission Assay, Electron Microscopy

Journal: International Journal of Environmental Research and Public Health

Article Title: A Muscarinic Antagonist Reduces Airway Inflammation and Bronchoconstriction Induced by Ambient Particulate Matter in a Mouse Model of Asthma

doi: 10.3390/ijerph15061189

Figure Lengend Snippet: Experimental protocol of the mouse model of ovalbumin (OVA)-induced asthma used in the present study. For details, please see the text. FORM, formoterol fumarate; FP, fluticasone propionate; NS, normal saline; PM, particulate matter; TIO, tiotropium bromide.

Article Snippet: To investigate the effect of drugs on airway inflammation and respiratory function, mice were treated with fluticasone propionate (Toronto Research Chemicals Inc., North York, ON, Canada), formoterol fumarate (Toronto Research Chemicals Inc.), or tiotropium bromide (Tokyo Chemical Industry Co., Ltd., Tokyo, Japan) on days 21 to 26.

Techniques: Saline

Journal: International Journal of Environmental Research and Public Health

Article Title: A Muscarinic Antagonist Reduces Airway Inflammation and Bronchoconstriction Induced by Ambient Particulate Matter in a Mouse Model of Asthma

doi: 10.3390/ijerph15061189

Figure Lengend Snippet: Effects of fluticasone propionate, formoterol fumarate, and tiotropium bromide on airway resistance. Airway resistance was evaluated by specific airway resistance (sRaw) values on day 27. Formoterol fumarate and tiotropium bromide decreased the sRaw values compared with fluticasone propionate. Data for each group are expressed as the mean ± standard deviation, with eight mice per group. * p < 0.05.

Article Snippet: To investigate the effect of drugs on airway inflammation and respiratory function, mice were treated with fluticasone propionate (Toronto Research Chemicals Inc., North York, ON, Canada), formoterol fumarate (Toronto Research Chemicals Inc.), or tiotropium bromide (Tokyo Chemical Industry Co., Ltd., Tokyo, Japan) on days 21 to 26.

Techniques: Standard Deviation

Journal: International Journal of Environmental Research and Public Health

Article Title: A Muscarinic Antagonist Reduces Airway Inflammation and Bronchoconstriction Induced by Ambient Particulate Matter in a Mouse Model of Asthma

doi: 10.3390/ijerph15061189

Figure Lengend Snippet: The levels of reactive oxygen metabolites after the administration of fluticasone propionate, formoterol fumarate, and tiotropium bromide. The levels of reactive oxygen metabolites (dROMs) in serum samples obtained on day 27. Fluticasone propionate and tiotropium bromide had no effect on dROM levels compared with the control group, but formoterol fumarate increased dROM levels. Data for each group are expressed as the mean ± standard deviation, with eight mice per group. * p < 0.05.

Article Snippet: To investigate the effect of drugs on airway inflammation and respiratory function, mice were treated with fluticasone propionate (Toronto Research Chemicals Inc., North York, ON, Canada), formoterol fumarate (Toronto Research Chemicals Inc.), or tiotropium bromide (Tokyo Chemical Industry Co., Ltd., Tokyo, Japan) on days 21 to 26.

Techniques: Control, Standard Deviation

Journal: Electrophoresis

Article Title: Liquid chromatographic method to follow-up ceftazidime and pyridine in portable elastomeric infusion pumps over 24 h.

doi: 10.1002/elps.202100275

Figure Lengend Snippet: Figure 1. Chemical structure of ceftazidime.

Article Snippet: Ceftazidime CRS and the -2 isomer were obtained from EDQM (Strasbourg, France) and pyridine from Fisher Scientific (Loughborough, UK).

Techniques:

Journal: Electrophoresis

Article Title: Liquid chromatographic method to follow-up ceftazidime and pyridine in portable elastomeric infusion pumps over 24 h.

doi: 10.1002/elps.202100275

Figure Lengend Snippet: Figure 2. Chromatogram obtained after injection of a forced degraded sample at 254 nm. (1) Pyridine, (2) Ethanal derivative, (3) -2 isomer, (4) Ceftazidime.

Article Snippet: Ceftazidime CRS and the -2 isomer were obtained from EDQM (Strasbourg, France) and pyridine from Fisher Scientific (Loughborough, UK).

Techniques: Injection

Journal: Electrophoresis

Article Title: Liquid chromatographic method to follow-up ceftazidime and pyridine in portable elastomeric infusion pumps over 24 h.

doi: 10.1002/elps.202100275

Figure Lengend Snippet: Figure 3. Linear regression performed on the percentages of cef- tazidime still present in one of the PEIPs at the different time points (starting dose: 6 g in 264 mL).

Article Snippet: Ceftazidime CRS and the -2 isomer were obtained from EDQM (Strasbourg, France) and pyridine from Fisher Scientific (Loughborough, UK).

Techniques:

Journal: Electrophoresis

Article Title: Liquid chromatographic method to follow-up ceftazidime and pyridine in portable elastomeric infusion pumps over 24 h.

doi: 10.1002/elps.202100275

Figure Lengend Snippet: Figure 5. Chromatograms obtained at 254 and 285 nm after injection of a sample taken from a PEIP at 20 h. (1) Pyridine, (2) Ethanal derivative, (3) Ceftazidime.

Article Snippet: Ceftazidime CRS and the -2 isomer were obtained from EDQM (Strasbourg, France) and pyridine from Fisher Scientific (Loughborough, UK).

Techniques: Injection

Journal: Agronomy

Article Title: Alleviating Continuous Cropping Obstacles in Celery Using Engineered Biochar: Insights into Chemical and Microbiological Aspects

doi: 10.3390/agronomy14112685

Figure Lengend Snippet: Figure 1. Changes in soil phenolic acid concentrations following the addition of 0% (N), 0.2% (BS2), 0.4% (BS4), or 0.8% (BS8) (w/w) WP400 biochar. * Indicates significant differences in the LSD test (p < 0.05) between treatments of the first crop. ☆:

Article Snippet: For the analysis, standard phenolic acids were used: 4-hydroxybenzoic acid (HYD), p-coumaric acid (COU) and ferulic acid (FER) from Sigma-Aldrich (St. Louis, MO, USA), and vanillic acid (VAN) from ChromaDex (Los Angeles, CA, USA).

Techniques: