Journal: Molecules

Article Title: Lipid Droplets as Cellular Sensors of Lipid Metabolic Reprogramming in Colon Cancer: Insights from Essential Amino Acid Supplementation Using Raman Spectroscopy and Imaging

doi: 10.3390/molecules31050762

Figure Lengend Snippet: Raman images of CCD-18Co cells for control cells and cells incubated with leucine, threonine, and arginine, with corresponding Raman spectra. The colors of spectra correspond to the colors of classes in the Raman maps and represent specific organelles: nucleus (red), lipid droplets (orange), endoplasmic reticulum (blue), mitochondria (magenta), cytoplasm (green), and membrane (light gray).

Article Snippet: CCD-18Co (ATCC ® CRL-1459TM) and Caco-2 cell lines were acquired from ATCC (Manassas, VA, USA) and cultured according to their standard procedures.

Techniques: Control, Incubation, Membrane

Journal: Molecules

Article Title: Lipid Droplets as Cellular Sensors of Lipid Metabolic Reprogramming in Colon Cancer: Insights from Essential Amino Acid Supplementation Using Raman Spectroscopy and Imaging

doi: 10.3390/molecules31050762

Figure Lengend Snippet: Raman images of CCD-18Co cells for control cells and cells incubated with leucine, threonine, and arginine obtained by the integration of the Raman bands over the characteristic spectral regions—organic matter (2820–3030 cm −1 ), nucleus (2935–3005 cm −1 ), saturated lipids (2820–2880 cm −1 ), and unsaturated lipids (3000–3030 cm −1 )—SAT/UNSAT lipid images, and microscope images.

Article Snippet: CCD-18Co (ATCC ® CRL-1459TM) and Caco-2 cell lines were acquired from ATCC (Manassas, VA, USA) and cultured according to their standard procedures.

Techniques: Control, Incubation, Microscopy

Journal: Molecules

Article Title: Lipid Droplets as Cellular Sensors of Lipid Metabolic Reprogramming in Colon Cancer: Insights from Essential Amino Acid Supplementation Using Raman Spectroscopy and Imaging

doi: 10.3390/molecules31050762

Figure Lengend Snippet: The average Raman spectra +/− SD (SD—standard deviation) in fingerprint region and in the high-frequency region for lipid droplets obtained for control CCD-18Co (blue line), CCD-18Co supplemented with leucine (olive line), CCD-18Co supplemented with threonine (magenta line), CCD-18Co supplemented with arginine (orange line), control Caco-2 (red line), Caco-2 supplemented with leucine (turquoise line), Caco-2 supplemented with threonine (green line), and Caco-2 supplemented with arginine (gray line). 20 mW laser power, 0.5 s integration time in the fingerprint region, and 0.3 s integration time in the high-frequency region.

Article Snippet: CCD-18Co (ATCC ® CRL-1459TM) and Caco-2 cell lines were acquired from ATCC (Manassas, VA, USA) and cultured according to their standard procedures.

Techniques: Standard Deviation, Control

Journal: Molecules

Article Title: Lipid Droplets as Cellular Sensors of Lipid Metabolic Reprogramming in Colon Cancer: Insights from Essential Amino Acid Supplementation Using Raman Spectroscopy and Imaging

doi: 10.3390/molecules31050762

Figure Lengend Snippet: PLS-DA score plots (model: mean center) for Raman spectra for lipid droplets for control CCD-18Co cell line (navy diamond), CCD-18Co cell line supplemented with leucine (blue circle), CCD-18Co cell line supplemented with threonine (ocean square), CCD-18Co cell line supplemented with arginine (turquoise pentagram) and for Raman spectra for control Caco-2 cell line (purple diamond), Caco-2 cell line supplemented with leucine (red circle), Caco-2 cell line supplemented with threonine (magenta square), Caco-2 cell line supplemented with arginine (light pink pentagram), and the loadings plots of LV1–LV3. One point in the PLS-DA score plot represents the average Raman spectrum of lipid droplets in one cell.

Article Snippet: CCD-18Co (ATCC ® CRL-1459TM) and Caco-2 cell lines were acquired from ATCC (Manassas, VA, USA) and cultured according to their standard procedures.

Techniques: Control

Journal: Molecules

Article Title: Lipid Droplets as Cellular Sensors of Lipid Metabolic Reprogramming in Colon Cancer: Insights from Essential Amino Acid Supplementation Using Raman Spectroscopy and Imaging

doi: 10.3390/molecules31050762

Figure Lengend Snippet: Response for lipid droplets of normal CCD-18Co (blue bar) colon cells to leucine (olive bar), threonine (magenta bar), and arginine (orange bar) supplementation and of cancer Caco-2 (red bar) colon cells to leucine (turquoise bar), threonine (green bar), and arginine (gray bar) supplementation. Normalized Raman intensities of the ratios: 2845/3015, 2845/2930, 3015/2888, and 1444/1256. *** means statistically significant results, p -value ≤ 0.001; ** means statistically significant results, p -value ≤ 0.01; * means statistically significant results, p -value ≤ 0.05. The number of cells for each culture condition is n = 10.

Article Snippet: CCD-18Co (ATCC ® CRL-1459TM) and Caco-2 cell lines were acquired from ATCC (Manassas, VA, USA) and cultured according to their standard procedures.

Techniques:

Journal: Journal of Dental Sciences

Article Title: Suppression of distal-less homeobox 3 increased alveolar bone mass in mouse tooth socket by targeting thrombospondin 1

doi: 10.1016/j.jds.2025.07.008

Figure Lengend Snippet: Earlier osteogenesis in extraction sockets of Dlx3 -knockdown mice. (a – f) Sagittal images of H&E stained maxillary incisal alveolar fossae from Dlx3 -knockdown and control mice at 7, 9, and 14 days after tooth extraction. Scale bars: 200 μm (a’-f’) Higher magnification of the dashed boxes in (a–f), showing the anterior 1/3 of the extraction socket. Scale: 50 μm. (g) Diagram depicting sagittal plane sectioning of the post-extraction alveolar socket. (h-k, h'-k') IHC analysis demonstrated expression of runt-related transcription factor 2(RUNX2) protein in extraction sockets of Control and Dlx3 -knockdown mice. Scale bars: (h–k): 50 μm; (h'-k'): 25 μm. (l) The ratio of RUNX2 + cells per total cells, results were expressed as mean ± SD, n = 3. ∗∗ P < 0.01, ∗∗∗ P < 0.001. Ctrl, control; Dlx3 , distal-less homeobox 3; RUNX2, runt-related transcription factor 2.

Article Snippet: For immunohistochemistry(IHC), deparaffinized sections underwent endogenous peroxidase blockade (3 % H 2 O 2 , 10 min) and blocking with 5 % goat serum (ZSGB-BIO, Beijing, China), followed by overnight incubation at 4 °C with primary antibodies: anti-runt-related transcription factor 2(RUNX2) (1:100; Proteintech, #20700-1-AP, Chicago, IL, USA) and anti-THBS1 (1:100; Proteintech, #18304-1-AP).

Techniques: Extraction, Knockdown, Staining, Control, Expressing