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Image Search Results
Journal: Cell Communication and Signaling : CCS
Article Title: Hydrogen sulfide induces regulatory B cells via glycolysis and mitochondrial ROS, attenuating LPS-induced lung injury
doi: 10.1186/s12964-025-02456-w
Figure Lengend Snippet: H 2 S promotes regulatory phenotypes of B cells under LPS stimulation. B cells stimulated with LPS (10 µg/ml) or LPS with NaHS on day 3 were used for analysis. A Representative plot of B220 and CD138 and total CD138 + cells were quantified ( n = 4). B B220 + CD138 + cells, B220 − CD138 + cells were quantified ( n = 4). C , D Cell-free supernatant on day 3 were analyzed by ELISA. NaHS (200 µM) was added during B cell culture. The levels of IgM ( C ) and IL-10 ( D ) were measured ( n = 6). E PD-L1 expression in each population (B220 + CD138 + , B220 − CD138 + ) with or without NaHS (200 µM) treatment measured by flow cytometry. The representative histogram and MFI of PD-L1 was shown ( n = 4). F - H siRNA administration was conducted after 48 h of initial LPS (10 µg/ml) stimulation. B cells were analyzed after 40 h of siRNA-transfection and secondary LPS (10 µg/ml) stimulation. F Representative plot of B220 and CD138 and each population (B220 + CD138 + , B220 − CD138 + ) were quantified ( n = 3). G The levels of IL-10 were measured by ELISA from cell-free supernatant ( n = 3). H Representative histogram and MFI of PD-L1 in each population ( n = 3). Data are representatives of three independent experiments and expressed as mean ± SD. Statistical significance was determined using Student’s t-test ( C ) or Welch’s t-test ( D ) or one-way ANOVA with Sidak’s multiple comparisons test ( A , B , H ) or with Tukey’s multiple comparisons test ( G , H ) or two-way ANOVA with Sidak’s multiple comparisons test ( E ). * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001
Article Snippet: Cbs and Cth knockdown were performed by specific
Techniques: Enzyme-linked Immunosorbent Assay, Cell Culture, Expressing, Flow Cytometry, Transfection