Journal: Frontiers in Immunology

Article Title: The β-NGF/TrkA Signalling Pathway Is Associated With the Production of Anti-Nucleoprotein IgG in Convalescent COVID-19

doi: 10.3389/fimmu.2021.813300

Figure Lengend Snippet: Multi-specific CD4 + and CD8 + T cell intracellular cytokine responses subsequent to peptide stimulation characterised according to PCR and antibody status. Percentage of (A) CD4 + and (B) CD8 + T cells respectively, producing IL-2, TNF-α, IFN-γ, and MIP-1β after 16-hour stimulation with selected peptide pools derived from SARS-CoV-2 (top); representative FACS plots stimulated with the corresponding peptide pools (bottom). Ctrl: cells were incubated with RPMI as a negative control. Percentage of subjects where (C) CD4 + and (D) CD8 + T cells produce the cytokines IL-2, TNF-α, IFN-γ, and MIP-1β after 16-hour stimulation with selected peptide pools within each group. (M: membrane; NP: nucleoprotein; ORF-7a-2: open reading frame 7a-2; S: spike). p- values determined by a two–way ANOVA with a Tukey’s post-hoc test for multiple comparisons. *p < 0.05; **p < 0.01; ***p < 0.001, ****p < 0.0001. (PCR-Ab- n=10; PCR+Ab+ N=10; PCR+Ab- n=7).

Article Snippet: Serum levels of a customised panel of cytokines and chemokines were determined using cytokine bead assay (CBA) kits (Bio-Techne Ltd) on a Magpix (Luminex Corporation) equipped with xPonent ® software for data acquisition and analysis.

Techniques: Derivative Assay, Incubation, Negative Control, Membrane

Journal: Frontiers in Immunology

Article Title: The β-NGF/TrkA Signalling Pathway Is Associated With the Production of Anti-Nucleoprotein IgG in Convalescent COVID-19

doi: 10.3389/fimmu.2021.813300

Figure Lengend Snippet: Differential T cells responses, pursuant to the number and type of cytokine produced within the different cohorts. (A) CD4 + and (B) CD8 + T cells producing cytokines after stimulation with the four peptide pools derived from SARs-CoV-2. The pie charts represent the proportion of subjects producing a different number of cytokines per group in response to each of the four peptide pools; the arcs show the production of each analysed cytokine, each corresponding to a different colour as indicated. (M, membrane; NP, nucleoprotein; ORF-7a-2, open reading frame 7a-2; S, spike). (PCR-Ab- n=9; PCR+Ab+ n=8; PCR+Ab- n=4).

Article Snippet: Serum levels of a customised panel of cytokines and chemokines were determined using cytokine bead assay (CBA) kits (Bio-Techne Ltd) on a Magpix (Luminex Corporation) equipped with xPonent ® software for data acquisition and analysis.

Techniques: Produced, Derivative Assay, Membrane

Journal: Frontiers in Immunology

Article Title: The β-NGF/TrkA Signalling Pathway Is Associated With the Production of Anti-Nucleoprotein IgG in Convalescent COVID-19

doi: 10.3389/fimmu.2021.813300

Figure Lengend Snippet: Distinct serum cytokine profiles in the subject cohorts and relative to antibody status. (A) Heatmap showing differential serum cytokine expression in the study subjects listed accordingly to respective anti-nucleoprotein IgG levels (Architect Index). (B) β-NGF and (C) IL-1α serum levels within the 3 different subject cohorts; boxes extend from the 25th to 75th percentiles, the line indicates the median, and the whiskers extend from the smaller to the largest values. Correlative expression of β-NGF with respect to (D) Architect index and (E) nAb levels, within the whole cohort. p-values in (B, C) were determined by a one-way ANOVA with a Tukey’s post-hoc test for multiple comparisons. A Spearman non-parametric correlation test was undertaken to test significance in (D, E) . *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; ns, not significant. (PCR-Ab- n=10; PCR+Ab+ N=10; PCR+Ab- n=7).

Article Snippet: Serum levels of a customised panel of cytokines and chemokines were determined using cytokine bead assay (CBA) kits (Bio-Techne Ltd) on a Magpix (Luminex Corporation) equipped with xPonent ® software for data acquisition and analysis.

Techniques: Expressing

Journal: Frontiers in Immunology

Article Title: The β-NGF/TrkA Signalling Pathway Is Associated With the Production of Anti-Nucleoprotein IgG in Convalescent COVID-19

doi: 10.3389/fimmu.2021.813300

Figure Lengend Snippet: Pearson correlation test between the Architect Index and serum cytokines levels.

Article Snippet: Serum levels of a customised panel of cytokines and chemokines were determined using cytokine bead assay (CBA) kits (Bio-Techne Ltd) on a Magpix (Luminex Corporation) equipped with xPonent ® software for data acquisition and analysis.

Techniques:

Journal: Frontiers in Immunology

Article Title: The β-NGF/TrkA Signalling Pathway Is Associated With the Production of Anti-Nucleoprotein IgG in Convalescent COVID-19

doi: 10.3389/fimmu.2021.813300

Figure Lengend Snippet: Global and antigen specific T cell expression of the β-NGF receptor TrkA, from the different cohorts. Correlative expression of β-NGF against MFI of TrkA + (A) CD4 + and (B) CD8 + T cells from all subjects studied. Summary data of percentage TrkA + (C) CD4 + and (D) CD8 + T cells (top), with representative FACS plots (bottom) from each cohort. Summary data of MFI expression of TrkA on (E) CD4 + and (F) CD8 + T cells (top); with representative MFI histograms from each cohort (bottom). MFI of TrkA + (G) CD4 + and (H) CD8 + T cells producing the respective cytokines after 16-hour stimulation with the four peptide pools derived from SARS-CoV-2. A Spearman non-parametric correlation test was undertaken to test significance in (A, B) , a one-ANOVA (C–F) and a two-way ANOVA (G, H) with a Tukey’s post-hoc test for multiple comparisons was used to demonstrate significance. Coloured lines for significance indicate changes relative to the corresponding cytokine. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; ns, not significant. (PCR-Ab- n=9; PCR+Ab+ n=8; PCR+Ab- n=4).

Article Snippet: Serum levels of a customised panel of cytokines and chemokines were determined using cytokine bead assay (CBA) kits (Bio-Techne Ltd) on a Magpix (Luminex Corporation) equipped with xPonent ® software for data acquisition and analysis.

Techniques: Expressing, Derivative Assay

Journal: Cerebral Cortex (New York, NY)

Article Title: Early Loss of Vision Results in Extensive Reorganization of Plasticity-Related Receptors and Alterations in Hippocampal Function That Extend Through Adulthood

doi: 10.1093/cercor/bhy297

Figure Lengend Snippet: Statistical comparison of neurotransmitter receptor expression in CBA/J and CBA/CaOlaHsd mice 2 and 4 months postnatally

Article Snippet: Male CBA/J (Charles River) and CBA/CaOlaHsd mice (Harlan (Envigo) Laboratories) were group housed in a temperature- and humidity-controled vivarium with a constant 12-h light–dark cycle (lights on from 6 AM to 6 PM) with ad libitum food and water access.

Techniques: Comparison, Expressing

Journal: Cell

Article Title: The striatum organizes 3D behavior via moment-to-moment action selection

doi: 10.1016/j.cell.2018.04.019

Figure Lengend Snippet: Key Resources Table

Article Snippet: pAAV-CBA-do(Fas)-GCaMP6s , This paper , Addgene ID 110135.

Techniques: Plasmid Preparation, Recombinant, Software