Journal: Journal of Cellular Physiology

Article Title: Cannabinoid Receptors Modulate Physiological Remodelling of the Blood–Testis Barrier

doi: 10.1002/jcp.70109

Figure Lengend Snippet: Gene expression analysis of CB1 and BTB components in WT and CB1 −/− (KO) testes. Western blot analysis of CB1, Vimentin (VIM), β‐catenin (β‐CTNN), β‐actin (β‐ACTN), F‐actin (F‐ACTN) and ZO‐1. Protein amounts were quantified by densitometry analysis, normalized against ERK1/2 signals and expressed in OD. All the data were reported as mean value ± S.E.M. (* p < 0.05; ** p < 0.01).

Article Snippet: CB1 , 1:1000 , Santa Cruz (sc‐293419), Texas, USA.

Techniques: Gene Expression, Western Blot

Journal: Journal of Cellular Physiology

Article Title: Cannabinoid Receptors Modulate Physiological Remodelling of the Blood–Testis Barrier

doi: 10.1002/jcp.70109

Figure Lengend Snippet: Histological analysis of seminiferous tubules from WT and CB1 −/− (KO) testes. H&E staining of WT and CB1 −/− (KO) testes: inset shows infiltrated blood cells (scale bar: 200 μm; inset scale bar: 10 μm) (A). Number of Stages VIII, IX and X–XI relatively to total tubules (B). Number of infiltrated tubules relatively to total tubules (C). Infiltrated tubules at Stages VIII, IX and X–XI relatively to the infiltration window (tubules at Stages VIII–XI) (D). All the data were reported as mean values ± S.E.M. (* p < 0.05; ** p < 0.01).

Article Snippet: CB1 , 1:1000 , Santa Cruz (sc‐293419), Texas, USA.

Techniques: Staining

Journal: Journal of Cellular Physiology

Article Title: Cannabinoid Receptors Modulate Physiological Remodelling of the Blood–Testis Barrier

doi: 10.1002/jcp.70109

Figure Lengend Snippet: Gene expression analysis of OCLN and markers related to its trafficking in WT and CB1 −/− (KO) testes. RTqPCR analysis of Ocln mRNA. Transcript amounts were reported as normalized fold expression (nfe) relatively to Rps18 gene (A). Western blot analysis of OCLN. Protein amounts were quantified by densitometry analysis, normalized against ERK1/2 signals and expressed in OD values (B). Immunofluorescence analysis of OCLN; white arrowheads represent OCLN localization (green) in testis. Nuclei were labelled with DAPI (blue). Scale bar: 5 µm (C). Quantitative analysis of OCLN green fluorescence on supra‐basal epithelium Regions of Interest (ROI); values are expressed as Sum of Intensity/area (SUM (I)/pixel 2 ), measured using the Nikon Imaging Analytical Software (NIS‐Elements Analysis D 4.40.00, 64 bit) (D). RTqPCR analysis of endocytic (Eea1), recycling (Rab13) and degradation (Rab9 and Itch) markers of OCLN. Transcript amounts were reported as normalized fold expression (nfe) relatively to Rps18 gene (E). All data were reported as mean value ± S.E.M. (* p < 0.05; ** p < 0.01).

Article Snippet: CB1 , 1:1000 , Santa Cruz (sc‐293419), Texas, USA.

Techniques: Gene Expression, Expressing, Western Blot, Immunofluorescence, Fluorescence, Imaging, Software

Journal: Journal of Cellular Physiology

Article Title: Cannabinoid Receptors Modulate Physiological Remodelling of the Blood–Testis Barrier

doi: 10.1002/jcp.70109

Figure Lengend Snippet: Responsiveness of OCLN expression to CB1 and CB2 receptors. Western blot analysis of OCLN in testis of HT mice in vitro treated with vehicle (CTRL), ACEA (A) or JWH‐133 (B) at different concentrations: 0.1–1–10 µM. Protein amounts were quantified by densitometry analysis, normalized against ERK1/2 signals and expressed in OD as fold change. Immunofluorescence analysis of OCLN in testis of CTRL and ACEA (10 µM) or JWH‐133 (1 µM); white arrowheads represent OCLN localization (green) in testis. Nuclei were labelled with DAPI (blue). Scale bar: 5 µm (C). Quantitative analysis of OCLN green fluorescence on supra‐basal epithelium Regions of Interest (ROI); values are expressed as Sum of Intensity/area (SUM (I)/pixel 2 ), measured using the Nikon Imaging Analytical Software (NIS‐Elements Analysis D 4.40.00, 64 bit) (D). RTqPCR analysis of Ocln mRNA in testis of CTRL and ACEA 10 µM (E) or JWH‐133 1 µM (F). Transcript amounts were reported as normalized fold expression (nfe) relatively to Rps18 gene. All the data were reported as mean value ± S.E.M. (* p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001; a vs. b: p < 0.05).

Article Snippet: CB1 , 1:1000 , Santa Cruz (sc‐293419), Texas, USA.

Techniques: Expressing, Western Blot, In Vitro, Immunofluorescence, Fluorescence, Imaging, Software

Journal: Journal of Cellular Physiology

Article Title: Cannabinoid Receptors Modulate Physiological Remodelling of the Blood–Testis Barrier

doi: 10.1002/jcp.70109

Figure Lengend Snippet: CB1 and CB2 action on BTB permeability and functionality. FITC‐dextran diffusion assay in testis of WT and HT mice in vitro treated with vehicle (CTRL), ACEA 10 µM and JWH‐133 1 µM (A) Scale bar: 300 μm. The green fluorescence signal diffused in the luminal compartment was quantified using the Nikon Imaging Analytical Software (NIS‐Elements Analysis D 4.40.00, 64 bit) as SUM (I)/pixel 2 (see also Figure for technical details) (B) a versus b: p < 0.05. RTqPCR analysis of endocytic (Eea1), recycling (Rab13) and degradation (Rab9 and Itch) markers of OCLN in the testis of CTRL and ACEA 10 µM (C) or JWH‐133 1 µM (D). Transcript amounts were reported as normalized fold expression (nfe) relatively to Rps18 gene. All data were reported as mean value ± S.E.M. (* p < 0.05; ** p < 0.01).

Article Snippet: CB1 , 1:1000 , Santa Cruz (sc‐293419), Texas, USA.

Techniques: Permeability, Diffusion-based Assay, In Vitro, Fluorescence, Imaging, Software, Expressing

Journal: bioRxiv

Article Title: Tetrahydrocannabinol exposure to postejaculatory sperm compromises sperm structure, function, the epigenome, and early embryo development

doi: 10.64898/2026.03.23.713385

Figure Lengend Snippet: A) Immunocytochemistry images of bovine sperm, human placenta, bovine placenta, and mouse brain depicting nuclear staining with DAPI (blue), CB1 (red), and merged images. FITC-PSA fluorescence can be observed in the merged image of sperm (green), for identification of the sperm acrosome. B) Western blot image of bovine sperm and equine pituitary identifying a single and specific band at the expected 75 kD value. Scale bar = 50 μM.

Article Snippet: Primary antibody incubation for detection of CB1 include the addition of 50 μL of Anti-CB1(proteintech ® Cat No: 17978-1-AP) at a dilution of 1:50 in BB for 15 h at 4 ° C. Slides were then washed and 100μL of secondary antibody (Donkey-AntiRabbit Abcam Cat No: ab6799) was added at a dilution of 1:100 (in BB) for 1 h in the dark followed by washing.

Techniques: Immunocytochemistry, Staining, Fluorescence, Western Blot

Journal: bioRxiv

Article Title: Tetrahydrocannabinol exposure to postejaculatory sperm compromises sperm structure, function, the epigenome, and early embryo development

doi: 10.64898/2026.03.23.713385

Figure Lengend Snippet: Localization of CB1 in non-capacitated sperm (upper panel), to the post-acrosomal sheath as compared to loss of abundance when sperm are held in capacitating conditions (middle panel) as indicted by tyrosine phosphorylation staining (YPO 3-, yellow). Sperm exposed to THC under non-capacitating conditions exhibit decreased YPO 3 , CB1 and acrosome detection (green) (lower panel). Scale bar = 50 μm.

Article Snippet: Primary antibody incubation for detection of CB1 include the addition of 50 μL of Anti-CB1(proteintech ® Cat No: 17978-1-AP) at a dilution of 1:50 in BB for 15 h at 4 ° C. Slides were then washed and 100μL of secondary antibody (Donkey-AntiRabbit Abcam Cat No: ab6799) was added at a dilution of 1:100 (in BB) for 1 h in the dark followed by washing.

Techniques: Phospho-proteomics, Staining