Journal: Cell Death & Disease

Article Title: Fallopian tube lesions as potential precursors of early ovarian cancer: a comprehensive proteomic analysis

doi: 10.1038/s41419-023-06165-5

Figure Lengend Snippet: Immunoreactivity and prognostic effect of proteins overexpressed in p53 signature compared to normal tissue according to Protein Atlas.

Article Snippet: Early prognosis validation was performed using antibodies directed against CAVIN2 (Rockland, 600-401-J31), Emilin2 (Thermo Scientific, pa584657), CAVIN1 (Abnova, H00284119-M02), SPTAN1 (OriGene, TA812019), and FBLN5 (Invitrogen, MA5-42544).

Techniques:

Journal: Cancer Growth and Metastasis

Article Title: A Role for the Cavin-3/Matrix Metalloproteinase-9 Signaling Axis in the Regulation of PMA-Activated Human HT1080 Fibrosarcoma Cell Neoplastic Phenotype

doi: 10.4137/CGM.S18581

Figure Lengend Snippet: PMA treatment triggers MMP-9 secretion and cavin-3 expression, but reduces the HT-1080 cell migration index. ( A ) Human HT-1080 fibrosarcoma cells were treated with varying concentrations of PMA in serum-free medium for 18 hours. Cells were then collected and analyzed for their migration capacity as described in the Methods section. ( B ) Total RNA was extracted from the remaining of the cells and qRT-PCR was performed to assess expression of cavin-1 , cavin-2 , cavin-3 and MMP-9 transcripts. Values were normalized over the housekeeping genes GAPDH and PPIγ and are the mean ± S.E.M of triplicate values from one out of three representative experiments. ( C ) Conditioned media was harvested to assess the extent of MMP-2 and MMP-9 gelatinolytic activity by zymography. ( D ) PMA-induced changes in endogenous cavin-3 protein expression were analyzed by Western blotting.

Article Snippet: C-terminal turboGFP-tagged full length cDNAs encoding human cavin-1, cavin-2, and cavin-3 were purchased from OriGene Technologies.

Techniques: Expressing, Migration, Quantitative RT-PCR, Activity Assay, Zymography, Western Blot

Journal: Cancer Growth and Metastasis

Article Title: A Role for the Cavin-3/Matrix Metalloproteinase-9 Signaling Axis in the Regulation of PMA-Activated Human HT1080 Fibrosarcoma Cell Neoplastic Phenotype

doi: 10.4137/CGM.S18581

Figure Lengend Snippet: Overexpression of recombinant cavin-3 specifically decreases cell migration and abrogates PMA-induced MMP-9 expression. ( A ) Human HT-1080 fibrosarcoma cells were transiently transfected with 2 μg of cDNA plasmids and fluorescent confocal microscopy was used to visualize the encoded recombinant GFP-tagged cavin-1, cavin-2 or cavin-3 proteins 24–48 hours post transfection. pEGFP cDNA plasmid was used as control. ( B ) Cells were then harvested and cell migration assayed as described in the Methods section. ( C ) HT-1080 cells were transiently transfected with plasmids as described above. Twenty-four hours post transfection, cells were treated with vehicle (white bars) or 100 nM PMA (Black bars) in a serum-free medium for 18 hours. Total RNA was extracted and qRT-PCR was performed to measure the levels of MMP-9 transcript. Values of PCR are normalized over the expression of housekeeping genes GAPDH and PPI γ and are the mean ± S.E.M of triplicate values from one representative experiment.

Article Snippet: C-terminal turboGFP-tagged full length cDNAs encoding human cavin-1, cavin-2, and cavin-3 were purchased from OriGene Technologies.

Techniques: Over Expression, Recombinant, Migration, Expressing, Transfection, Confocal Microscopy, Plasmid Preparation, Quantitative RT-PCR