Journal: Journal of Biomedicine and Biotechnology

Article Title: Proteomic Analysis of Pichindé virus Infection Identifies Differential Expression of Prothymosin- α

doi: 10.1155/2010/956823

Figure Lengend Snippet: Identification and validation of peak 3790 as prothymosin- α . Mass spectrum of proteins from fraction Q3 that bound to Q10 ProteinChip array and was analyzed on QSTAR XL instrument (mass range from 1000 to 4000 m/z).

Article Snippet: 10 μ L of each fraction was added to 90 μ L 50 mM Tris (pH 8) buffer and was captured on Q10 anion exchange ProteinChip array surfaces (Bio-Rad Laboratories, Hercules, CA) with a bioprocessor Biomek 3000 (Beckman Coulter, Fullerton, CA).

Techniques: Biomarker Discovery

Journal: Journal of Biomedicine and Biotechnology

Article Title: Proteomic Analysis of Pichindé virus Infection Identifies Differential Expression of Prothymosin- α

doi: 10.1155/2010/956823

Figure Lengend Snippet: (a) The peptide with m/z approximately 3790 was identified as the fragment of prothymosin alpha by the following MS/MS microsequencing. (b) Mass spectrum of proteins pulled out from cytoplasmic fraction of murine macrophage samples by antiprothymosin alpha which was coupled to protein G agarose beads (mass range from 1000 to 20,000 m/z). Antiprothymosin alpha-coupled protein G agarose beads were incubated with cytoplasmic fraction and eluted proteins were analyzed on NP 20 ProteinChip array.

Article Snippet: 10 μ L of each fraction was added to 90 μ L 50 mM Tris (pH 8) buffer and was captured on Q10 anion exchange ProteinChip array surfaces (Bio-Rad Laboratories, Hercules, CA) with a bioprocessor Biomek 3000 (Beckman Coulter, Fullerton, CA).

Techniques: Tandem Mass Spectroscopy, Incubation

Journal: Diagnostic Pathology

Article Title: Comparative proteomic analysis of normal and tumor stromal cells by tissue on chip based mass spectrometry (toc-MS)

doi: 10.1186/1746-1596-5-10

Figure Lengend Snippet: Principle of tissue on chip based mass spectrometry (toc-MS): (A) Head and neck cancer (HNC) tissue sections were stained H&E to obtain an overview of the tissue architecture . (B) Exemplary cutting lines of laser microdissection. (C) Stroma areas with about 100 square μm were cut out using the laser microdissection and transferred on a ProteinChip array (D). The same procedure was performed with normal connective tissue (not to scale).

Article Snippet: A Q10 ProteinChip array (strong anion exchanger; BioRad) was activated (see [ ]) and wetted with 0.5 μl lysis buffer (100 mM Na-phosphate (pH 7.5), 5 mM EDTA, 2 mM MgCl 2 , 3 mM 2-β-mercaptoethanol, 0.1% CHAPS, 500 μM leupeptine, and 0.1 mM PMSF).

Techniques: Mass Spectrometry, Staining, Laser Capture Microdissection