source 15s cation exchange column (GE Healthcare)

GE Healthcare source 15s cation exchange column
Source 15s Cation Exchange Column, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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weak cation exchange cm10 proteinchip arrays (Bio-Rad)

Bio-Rad weak cation exchange cm10 proteinchip arrays
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Bio-Rad cation exchange chromatographic surface
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cm10 microscope (Philips Healthcare)

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cm10 sequence lianak (GenScript corporation)

GenScript corporation cm10 sequence lianak

List of experimental groups and concentrations evaluated.

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Image Search Results

Journal: Osteoarthritis and Cartilage Open

Article Title: Comparison of multiple synthetic chondroinductive factors in pellet culture against a TGF-β positive control

doi: 10.1016/j.ocarto.2023.100369

Figure Lengend Snippet: List of experimental groups and concentrations evaluated.

Article Snippet: For experimental groups, KGN (Cat# HY-16268, MedChemExpress, Monmouth Junction, NJ), SM04690 (Cat# HY-109049, MedChemExpress, Monmouth Junction, NJ), CM10 (Sequence LIANAK, Genscript, New Jersey), and/or CK2.1 (Sequence: QIKIWFQNRRK-WKKMVPSDPSYEDMGGC, Genscript, New Jersey) were added to the control medium at concentrations that were based on the literature and summarized in .

Techniques:

Chondrogenic gene expression of ACAN in hBMSCs spheroids after 21 days in culture. No statistically significant differences were observed between groups except for TGF-β3, which had significantly higher aggrecan expression compared to all other groups, including a 10,000-fold larger value compared to the negative control. ∗∗∗∗Refers to significant difference p < 0.0001, n = 6–8. Reported values are mean + standard deviation. SM = SM04690, CK = CK2.1, CM = CM10, CM/CK (100/100) = CM10 at 100 μM and CK2.1 and 100 nM, CM/CK(100/500) = CM10 at 100 μM and CK2.1 and 500 nM, CM/CK (200/100) = CM10 at 200 μM and CK2.1 and 100 nM, CM/CK(200/500) = CM10 at 200 μM and CK2.1 and 500 nM, CM/KGN (100/1) = CM10 at 100 μM and kartogenin at 1 μM, CM/KGN (100/10) = CM10 at 100 μM and kartogenin at 10 μM, CK/KGN (100/1) = CK2.1 at 100 nM and kartogenin at 1 μM, CK/KGN (100/10) = CK2.1 at 100 nM and kartogenin at 10 μM, CM/SM (100/30) = CM10 at 100 μM and SM04690 at 30 nM, CM/SM (100/100) = CM10 at 100 μM and SM04690 at 100 nM, CK/SM (100/30) = CK2.1 at 100 nM and SM04690 at 30 nM, CK/SM (100/100) = CK2.1 at 100 nM and SM04690 at 100 nM.

Journal: Osteoarthritis and Cartilage Open

Article Title: Comparison of multiple synthetic chondroinductive factors in pellet culture against a TGF-β positive control

doi: 10.1016/j.ocarto.2023.100369

Figure Lengend Snippet: Chondrogenic gene expression of ACAN in hBMSCs spheroids after 21 days in culture. No statistically significant differences were observed between groups except for TGF-β3, which had significantly higher aggrecan expression compared to all other groups, including a 10,000-fold larger value compared to the negative control. ∗∗∗∗Refers to significant difference p < 0.0001, n = 6–8. Reported values are mean + standard deviation. SM = SM04690, CK = CK2.1, CM = CM10, CM/CK (100/100) = CM10 at 100 μM and CK2.1 and 100 nM, CM/CK(100/500) = CM10 at 100 μM and CK2.1 and 500 nM, CM/CK (200/100) = CM10 at 200 μM and CK2.1 and 100 nM, CM/CK(200/500) = CM10 at 200 μM and CK2.1 and 500 nM, CM/KGN (100/1) = CM10 at 100 μM and kartogenin at 1 μM, CM/KGN (100/10) = CM10 at 100 μM and kartogenin at 10 μM, CK/KGN (100/1) = CK2.1 at 100 nM and kartogenin at 1 μM, CK/KGN (100/10) = CK2.1 at 100 nM and kartogenin at 10 μM, CM/SM (100/30) = CM10 at 100 μM and SM04690 at 30 nM, CM/SM (100/100) = CM10 at 100 μM and SM04690 at 100 nM, CK/SM (100/30) = CK2.1 at 100 nM and SM04690 at 30 nM, CK/SM (100/100) = CK2.1 at 100 nM and SM04690 at 100 nM.

Techniques: Gene Expression, Expressing, Negative Control

Chondrogenic gene expression of SOX-9 in hBMSCs spheroids after 21 days in culture. No significance was observed between groups except for TGF-β3 with a 50-fold higher gene expression as compared to the negative control. ∗∗∗∗Refers to significant difference p < 0.0001, n = 6–8. Reported values are mean + standard deviation. SM = SM04690, CK = CK2.1, CM = CM10, CM/CK (100/100) = CM10 at 100 μM and CK2.1 and 100 nM, CM/CK(100/500) = CM10 at 100 μM and CK2.1 and 500 nM, CM/CK (200/100) = CM10 at 200 μM and CK2.1 and 100 nM, CM/CK(200/500) = CM10 at 200 μM and CK2.1 and 500 nM, CM/KGN (100/1) = CM10 at 100 μM and kartogenin at 1 μM, CM/KGN (100/10) = CM10 at 100 μM and kartogenin at 10 μM, CK/KGN (100/1) = CK2.1 at 100 nM and kartogenin at 1 μM, CK/KGN (100/10) = CK2.1 at 100 nM and kartogenin at 10 μM, CM/SM (100/30) = CM10 at 100 μM and SM04690 at 30 nM, CM/SM (100/100) = CM10 at 100 μM and SM04690 at 100 nM, CK/SM (100/30) = CK2.1 at 100 nM and SM04690 at 30 nM, CK/SM (100/100) = CK2.1 at 100 nM and SM04690 at 100 nM.

Journal: Osteoarthritis and Cartilage Open

Article Title: Comparison of multiple synthetic chondroinductive factors in pellet culture against a TGF-β positive control

doi: 10.1016/j.ocarto.2023.100369

Figure Lengend Snippet: Chondrogenic gene expression of SOX-9 in hBMSCs spheroids after 21 days in culture. No significance was observed between groups except for TGF-β3 with a 50-fold higher gene expression as compared to the negative control. ∗∗∗∗Refers to significant difference p < 0.0001, n = 6–8. Reported values are mean + standard deviation. SM = SM04690, CK = CK2.1, CM = CM10, CM/CK (100/100) = CM10 at 100 μM and CK2.1 and 100 nM, CM/CK(100/500) = CM10 at 100 μM and CK2.1 and 500 nM, CM/CK (200/100) = CM10 at 200 μM and CK2.1 and 100 nM, CM/CK(200/500) = CM10 at 200 μM and CK2.1 and 500 nM, CM/KGN (100/1) = CM10 at 100 μM and kartogenin at 1 μM, CM/KGN (100/10) = CM10 at 100 μM and kartogenin at 10 μM, CK/KGN (100/1) = CK2.1 at 100 nM and kartogenin at 1 μM, CK/KGN (100/10) = CK2.1 at 100 nM and kartogenin at 10 μM, CM/SM (100/30) = CM10 at 100 μM and SM04690 at 30 nM, CM/SM (100/100) = CM10 at 100 μM and SM04690 at 100 nM, CK/SM (100/30) = CK2.1 at 100 nM and SM04690 at 30 nM, CK/SM (100/100) = CK2.1 at 100 nM and SM04690 at 100 nM.

Techniques: Gene Expression, Negative Control

Gene expression of collagen I of hBMSCs spheroids after 21 days in culture. A variation in the gene expression of collagen I by hBMSCs was observed in response to the evaluated compounds and peptides. CM10 (100 μM) had the highest gene expression with 2.5-fold higher expression compared to the control group. CK2.1 exhibited higher gene expression of collagen I by 1.8 folds and 1.4 folds at 100 nM and 500 nM, respectively. The combination of compounds and peptides did not induce a significant difference in collagen I gene expression as compared to the negative control group. Groups with the same letters indicate no significance, n = 6–8. Reported values are mean + standard deviation. SM = SM04690, CK = CK2.1, CM = CM10, CM/CK (100/100) = CM10 at 100 μM and CK2.1 and 100 nM, CM/CK(100/500) = CM10 at 100 μM and CK2.1 and 500 nM, CM/CK (200/100) = CM10 at 200 μM and CK2.1 and 100 nM, CM/CK(200/500) = CM10 at 200 μM and CK2.1 and 500 nM, CM/KGN (100/1) = CM10 at 100 μM and kartogenin at 1 μM, CM/KGN (100/10) = CM10 at 100 μM and kartogenin at 10 μM, CK/KGN (100/1) = CK2.1 at 100 nM and kartogenin at 1 μM, CK/KGN (100/10) = CK2.1 at 100 nM and kartogenin at 10 μM, CM/SM (100/30) = CM10 at 100 μM and SM04690 at 30 nM, CM/SM (100/100) = CM10 at 100 μM and SM04690 at 100 nM, CK/SM (100/30) = CK2.1 at 100 nM and SM04690 at 30 nM, CK/SM (100/100) = CK2.1 at 100 nM and SM04690 at 100 nM.

Journal: Osteoarthritis and Cartilage Open

Article Title: Comparison of multiple synthetic chondroinductive factors in pellet culture against a TGF-β positive control

doi: 10.1016/j.ocarto.2023.100369

Figure Lengend Snippet: Gene expression of collagen I of hBMSCs spheroids after 21 days in culture. A variation in the gene expression of collagen I by hBMSCs was observed in response to the evaluated compounds and peptides. CM10 (100 μM) had the highest gene expression with 2.5-fold higher expression compared to the control group. CK2.1 exhibited higher gene expression of collagen I by 1.8 folds and 1.4 folds at 100 nM and 500 nM, respectively. The combination of compounds and peptides did not induce a significant difference in collagen I gene expression as compared to the negative control group. Groups with the same letters indicate no significance, n = 6–8. Reported values are mean + standard deviation. SM = SM04690, CK = CK2.1, CM = CM10, CM/CK (100/100) = CM10 at 100 μM and CK2.1 and 100 nM, CM/CK(100/500) = CM10 at 100 μM and CK2.1 and 500 nM, CM/CK (200/100) = CM10 at 200 μM and CK2.1 and 100 nM, CM/CK(200/500) = CM10 at 200 μM and CK2.1 and 500 nM, CM/KGN (100/1) = CM10 at 100 μM and kartogenin at 1 μM, CM/KGN (100/10) = CM10 at 100 μM and kartogenin at 10 μM, CK/KGN (100/1) = CK2.1 at 100 nM and kartogenin at 1 μM, CK/KGN (100/10) = CK2.1 at 100 nM and kartogenin at 10 μM, CM/SM (100/30) = CM10 at 100 μM and SM04690 at 30 nM, CM/SM (100/100) = CM10 at 100 μM and SM04690 at 100 nM, CK/SM (100/30) = CK2.1 at 100 nM and SM04690 at 30 nM, CK/SM (100/100) = CK2.1 at 100 nM and SM04690 at 100 nM.

Techniques: Gene Expression, Expressing, Control, Negative Control

Gene expression of ACAN, SOX-9, and collagen I of hBMSCs spheroids from 3 different donors after 21 days in culture. For ACAN, no statistically significant differences were observed between groups except for TGF-β3 with all donors. Donor-based variation in the gene expression of SOX-9 in response to experimental groups was observed. Notably, CM10 had a significantly higher gene expression of SOX-9 as compared to the negative control only with donor 1 (Female, 20 yrs). SM04690, CM10, and CK2.1 induced a significantly lower gene expression of collagen I as compared to the negative control with all donors, whereas KGN had variable outcomes based on the donor. Groups with the same letters indicate no significance, n = 6–8. Reported values are mean + standard deviation. SM = SM04690, CK = CK2.1, CM = CM10.

Journal: Osteoarthritis and Cartilage Open

Article Title: Comparison of multiple synthetic chondroinductive factors in pellet culture against a TGF-β positive control

doi: 10.1016/j.ocarto.2023.100369

Figure Lengend Snippet: Gene expression of ACAN, SOX-9, and collagen I of hBMSCs spheroids from 3 different donors after 21 days in culture. For ACAN, no statistically significant differences were observed between groups except for TGF-β3 with all donors. Donor-based variation in the gene expression of SOX-9 in response to experimental groups was observed. Notably, CM10 had a significantly higher gene expression of SOX-9 as compared to the negative control only with donor 1 (Female, 20 yrs). SM04690, CM10, and CK2.1 induced a significantly lower gene expression of collagen I as compared to the negative control with all donors, whereas KGN had variable outcomes based on the donor. Groups with the same letters indicate no significance, n = 6–8. Reported values are mean + standard deviation. SM = SM04690, CK = CK2.1, CM = CM10.

Techniques: Gene Expression, Negative Control

cation exchange cm10 array Search Results

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