Journal: PLOS ONE

Article Title: Expression of RNA polymerase I catalytic core is influenced by RPA12

doi: 10.1371/journal.pone.0285660

Figure Lengend Snippet: (A) qPCR analysis of RPA12 transcript in A375 melanoma cells with transient RPA12 knockdown using siRNA. Mean fold change ± SD of n = 4 biological replicates is shown. Mann-Whitney two-tailed t-test *, p < 0.05. (B) Immunofluorescence staining of RPA12. Representative biological replicate of n = 4 is shown. (C) Quantification of the images for RPA12. Mean fold change ±SD of n = 3 biological replicates is shown. Analysis of N = 50 cells per treatment. Student’s two-tailed t-test **, p = 0.0018. (D) Immunofluorescence staining of siCtrl and siRPA12 knockdown cells. A375 melanoma cells were stained for the indicated Pol I subunits and fibrillarin (FBL) following siRNA knockdown of RPA12. DNA was counterstained using Hoechst. Representative biological replicates of n = 4 are shown. (E) Quantification of the images for RPA194. Mean fold change ±SD of n = 3 biological replicates is shown. N = 50–100 cells per treatment. Student’s two-tailed t-test *, p = 0.0306. (F) Immunofluorescence staining of siCtrl and siRPA12 knockdown cells for PAF53 and CAST. (G) Quantification of the images for PAF53 and CAST. N = over 100 cells per treatment. Scale bars, 10 μm.

Article Snippet: The following primary antibodies were used: RPA135 (mouse monoclonal 4H6 1:100; sc-293272 Santa Cruz Biotechnology), RPA194 (mouse monoclonal C-1 1:100; sc-48385 Santa Cruz Biotechnology), PAF53 (rabbit polyclonal 16145-1-AP 1:200; ProteinTech), CAST (rabbit polyclonal PA5-27632 1:200; Bethyl Laboratories), ZNRD1 (RPA12) (mouse monoclonal D10 1:100; sc-393406 Santa Cruz Biotechnology) and fibrillarin (rabbit polyclonal 1:500; ab5821 Abcam).

Techniques: Knockdown, MANN-WHITNEY, Two Tailed Test, Immunofluorescence, Staining

Journal: The Journal of Physiology

Article Title: Statin myalgia is not associated with reduced muscle strength, mass or protein turnover in older male volunteers, but is allied with a slowing of time to peak power output, insulin resistance and differential muscle mRNA expression

doi: 10.1113/jphysiol.2014.285577

Figure Lengend Snippet: List genes used on low density RT‐PCR array microfludic cards (Applied Biosystems Inc., Foster City, CA, USA)

Article Snippet: Proteolysis , CAST , Calpastatin , Hs00156280_m1.

Techniques: Binding Assay, Phospho-proteomics, Ubiquitin Proteomics

Journal: Cell

Article Title: Structural basis for the assembly of the type V CRISPR-associated transposon complex

doi: 10.1016/j.cell.2022.11.009

Figure Lengend Snippet:

Article Snippet: The pDonor (Addgene #127921), pHelper (Addgene #127924), and pTarget (Addgene #127926) plasmids used in droplet digital PCR experiments were sourced from Addgene.

Techniques: Recombinant, Mass Spectrometry, Electron Microscopy, Plasmid Preparation, Virus, Cloning, Software