Journal: Journal of Biological Chemistry

Article Title: Probing Conformational Rescue Induced by a Chemical Corrector of F508del-Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) Mutant

doi: 10.1074/jbc.m111.239699

Figure Lengend Snippet: FIGURE 5. The effect of chemical correction and ablation of the R553XR555 sequence on conformational correction of F508del-CFTR probed using limited proteolysis. Limited proteolysis of control or chemically corrected F508del-CFTR, F508del-KXK-CFTR, or WT-CFTR with increasing levels of trypsin (0, 1.56, 3.13, 6.25, 12.5, 25, 50, 100 g/ml) was performed. Digests were analyzed by SDS-PAGE, and immunoreactive bands were detected using the Odyssey infrared imaging system from LI-COR Biosciences. The colored signal was converted to grayscale for the preparation of this figure. A, samples were immuno- blotted with anti-CFTR monoclonal antibody, L12B4 (NBD1-specific). Subsequent analyses focused on the larger NBD1 fragments (34–36 kDa) and the smaller NBD1 fragments (lower than 28 kDa). Both sets of fragments were indicated using brackets on the right-hand side of each image. B, samples were immuno- blotted with anti-CFTR monoclonal antibody, M3A7 (NBD2-specific). NBD2 fragments of 28 kDa were analyzed subsequently (indicated using brackets on the right-hand side of each image).

Article Snippet: L12B4 anti-CFTR antibody (1:1000) was used for immunodetection conjugated to IRDye800 (1:10 000) (Rockland Immunochemical Inc).

Techniques: Sequencing, Control, SDS Page, Imaging

Journal: Viruses

Article Title: Hepatitis C Virus-Induced FUT8 Causes 5-FU Drug Resistance in Human Hepatoma Huh7.5.1 Cells

doi: 10.3390/v11040378

Figure Lengend Snippet: Inhibition of P-gp and MRP1 increased the 5-FU drug sensitivity after HCV infection. ( A , C ) Western blot analysis of P-gp and MRP1 expression in Huh7.5.1 cells in the presence or absence of NSC23925 (P-gp inhibitor) and MK571 (MRP1 inhibitor). Western blot quantification analysis is also listed as ( B , D ). ( E – H ) Analysis of the cytotoxicity of drugs (5-FU/MTX/ADR/CDDP) in HCVcc-infected Huh7.5.1 cells in the presence of P-gp and MRP1 inhibitors.

Article Snippet: P-glycoprotein (P-gp) inhibitor NSC23925 and multidrug resistance-associated protein-1 (MRP1) inhibitor MK571 were purchased from MedChemExpress (MCE) (Monmouth Junction, NJ, USA) and dissolved in dimethyl sulfoxide (DMSO).

Techniques: Inhibition, Infection, Western Blot, Expressing