Journal: Biomolecules

Article Title: Targeted Regulation of HSP70 by the ARP2/3 Complex in Mammary Epithelial Cells and Its Impact on Host Cell Apoptosis

doi: 10.3390/biom15040538

Figure Lengend Snippet: The effect of LPS treatment on the expression of apoptosis-related factors in MAC-T cells. ( A –C) Relative mRNA ( A ) and protein ( B , C ) expression levels of Caspase3, Caspase7, Caspase8, Bax, and Bcl-2 in MAC-T cells following LPS treatment. ( D – F ) Relative mRNA ( D ) and protein ( E , F ) expression levels of Caspase3, Caspase7, Caspase8, Bax, and Bcl-2 in mouse mammary tissue following LPS treatment (n = 3). Statistical significance was determined by comparison with the control group (* p < 0.05; ** p < 0.01). Note: CM, clinical mastitis.

Article Snippet: The membranes were blocked with rapid Sealing Solution (P0222, Beyotime, Shanghai, China) for 10–20 min at room temperature and incubated 10–12 h at 4 °C with primary antibodies against ARPC3, ARPC4, Bax, Bcl-2, HSPA1A, HSPA1L, Caspase7, IL-1β, TNF-α, IL-6, β-actin (Proteintech, Wuhan, China), IL-8 (Affinity Biosciences, Cincinnati, OH, USA), Caspase3, and Caspase8 (Bioss, Beijing, China).

Techniques: Expressing, Comparison, Control

Journal: Biomolecules

Article Title: Targeted Regulation of HSP70 by the ARP2/3 Complex in Mammary Epithelial Cells and Its Impact on Host Cell Apoptosis

doi: 10.3390/biom15040538

Figure Lengend Snippet: Effect of ARPC3/ARPC4 inhibition on HSP70 expression and cell apoptosis. ( A – C ) Relative protein and mRNA expression levels of ARPC3/ARPC4, HSP70, Caspase3, and Caspase7 in LPS-induced MAC-T cells following CK666 treatment. ( D ) Flow cytometry analysis of apoptosis rates in cells treated with CK666 and LPS. ( E ) Localization of ARPC3/ARPC4 expression in MAC-T cells (scale: 120 μm) (n = 3). All micrographs share identical magnification parameters. A representative scale bar (120 μm) is depicted in the higher left corner of panel E. Statistical significance was determined by comparison with the LPS + DMSO group ( ** p < 0.01).

Article Snippet: The membranes were blocked with rapid Sealing Solution (P0222, Beyotime, Shanghai, China) for 10–20 min at room temperature and incubated 10–12 h at 4 °C with primary antibodies against ARPC3, ARPC4, Bax, Bcl-2, HSPA1A, HSPA1L, Caspase7, IL-1β, TNF-α, IL-6, β-actin (Proteintech, Wuhan, China), IL-8 (Affinity Biosciences, Cincinnati, OH, USA), Caspase3, and Caspase8 (Bioss, Beijing, China).

Techniques: Inhibition, Expressing, Flow Cytometry, Comparison