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Image Search Results
Journal: The Journal of Veterinary Medical Science
Article Title: Compensatory Cellular Reactions to Nonsteroidal Anti-Inflammatory Drugs on Osteogenic Differentiation in Canine Bone Marrow-Derived Mesenchymal Stem Cells
doi: 10.1292/jvms.13-0482
Figure Lengend Snippet: Effects of carprofen (A), meloxicam (B), indomethacin (C) and robenacoxib (D) on viability and PGE 2 synthesis in canine bone marrow-derived mesenchymal stem cells. Cell viability was determined using MTT assay after drug treatment for 48 hr. Production of PGE 2 (C) was quantified by ELISA after 24 hr of cell culture with 0.1, 1 and 10 µ M of NSAIDs. Data are means ± SD from three independent experiments that were performed in triplicate. * p <0.05.
Article Snippet: Cell viability assay :
Techniques: Derivative Assay, MTT Assay, Enzyme-linked Immunosorbent Assay, Cell Culture
Journal: The Journal of Veterinary Medical Science
Article Title: Compensatory Cellular Reactions to Nonsteroidal Anti-Inflammatory Drugs on Osteogenic Differentiation in Canine Bone Marrow-Derived Mesenchymal Stem Cells
doi: 10.1292/jvms.13-0482
Figure Lengend Snippet: Effects of NSAIDs on differentiation of osteoblasts in canine bone marrow-derived mesenchymal stem cells. Gene expression (A, D) was analyzed using quantitative real-time polymerase chain reaction. Enzyme activity of ALP (B) was measured by colorimetric assay using p -nitrophenylphosphate. Morphological analysis (C) was performed using ALP staining. Level of calcification (E) was quantified by Alizarin red S assay on day 20. Expression levels of ALP (A, B and C) were analyzed on day 4. In groups for recovery, 10 nM of PGE 2 was added (C and E). Car, carprofen (10 µ M); Mel: meloxicam (10 µ M); Ind, indomethacin (10 µ M); Rob, robenacoxib (10 µ M). Data are means ± SD (n=3). * p <0.05; † p <0.05 between 2 columns.
Article Snippet: Cell viability assay :
Techniques: Derivative Assay, Gene Expression, Real-time Polymerase Chain Reaction, Activity Assay, Colorimetric Assay, Staining, Expressing
Journal: The Journal of Veterinary Medical Science
Article Title: Compensatory Cellular Reactions to Nonsteroidal Anti-Inflammatory Drugs on Osteogenic Differentiation in Canine Bone Marrow-Derived Mesenchymal Stem Cells
doi: 10.1292/jvms.13-0482
Figure Lengend Snippet: Compensatory response under NSAID treatment during osteoblast differentiation in canine bone marrow-derived mesenchymal stem cells. Gene expression was analyzed on day 4 using quantitative real-time polymerase chain reaction (A and B). Production of PGE 2 (C) was quantified by ELISA. Car, carprofen (10 µ M); Mel, meloxicam (10 µ M); Ind, indomethacin (10 µ M); Rob, robenacoxib (10 µ M). Data are means ± SD (n=3). * p <0.05.
Article Snippet: Cell viability assay :
Techniques: Derivative Assay, Gene Expression, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay