Journal: BMC Genomics

Article Title: Water deficit alters differentially metabolic pathways affecting important flavor and quality traits in grape berries of Cabernet Sauvignon and Chardonnay

doi: 10.1186/1471-2164-10-212

Figure Lengend Snippet: The influence of water deficit on transcript abundance NCED1 and NCED2 using qRT-PCR and other ABA-related transcripts on the Vitis Genome Array . Data were normalized to the transcript abundance of an ankyrin-repeat protein (1612584_s_at) that did not change with developmental stage or stress treatment. The lines and symbols are the same as Figure 1; n = 3. NCED1 (nine-cis-epoxycarotenoid dioxygenase 1), 1608022_at, TC57089, AY337613; NCED2 (nine-cis-epoxycarotenoid dioxygenase 2), TC71235, AY337614; bZIP TF (homeobox-leucine zipper transcription factor), 1609295_at.

Article Snippet: Downstream of BHASE, a carotenoid cleavage dioxygenase (CCD) known to increase at véraison [ ], cleaves zeaxanthin into a C13 -norisoprenoid and a C14 -dialdehyde, both volatile compounds.

Techniques: Quantitative RT-PCR

Journal: Biomolecules

Article Title: Expression of a Chromoplast-Specific Lycopene β-Cyclase Gene (CYC-B) Is Implicated in Carotenoid Accumulation and Coloration in the Loquat

doi: 10.3390/biom9120874

Figure Lengend Snippet: qRT-PCR analysis of genes related to carotenoid metabolic pathways after silencing. Plastids are the sites for the biosynthesis and accumulation of plant carotenoids. PAP, plastid lipid-associated protein; PSY, phytoene synthase; PDS, phytoene desaturase; ZDS, ζ-carotene desaturase; ZISO, 15-cis-ζ-carotene isomerase; CRTISO, carotene isomerase; LCYE, lycopene ε-cyclase; LCYB, lycopene β-cyclase; CYC-B, chromoplast-specific lycopene β-cyclase; BCH, β-carotene hydroxylase; ECH, ε-carotene hydroxylase; ZEP, zeaxanthin epoxidase; VDE, violaxanthin de-epoxidase; NXY, neoxanthin synthase; CCD, carotenoid cleavage dioxygenases. Different letters indicate significant differences between negative control and silenced fruits (One-way ANOVA, p

Article Snippet: Carotenoid cleavage dioxygenases (CCD) and nine-cis-epoxycarotenoid dioxygenase (NCED) are involved in the carotenoid cleavage pathway in plants.

Techniques: Quantitative RT-PCR, Negative Control

Journal: PLoS ONE

Article Title: Establishment of an Arabidopsis callus system to study the interrelations of biosynthesis, degradation and accumulation of carotenoids

doi: 10.1371/journal.pone.0192158

Figure Lengend Snippet: Carotenoid synthesis and cleavage pathway in higher plants. Enzymes (in bold) and corresponding Arabidopsis mutant names (in italics) are given; carotenoids accumulating in WT tissues are underlined; boxes indicate colour code used for carotenoids in subsequent figures. GGPP, geranylgeranyl diphosphate; PSY, phytoene synthase; PDS, phytoene desaturase; Z-ISO, ζ-carotene isomerase; ZDS, ζ-carotene desaturase; CRTISO, carotenoid isomerase; eLCY, ε-cyclase; bLCY, β-cyclase; CYPA3, cytP450 hydroxylase 97A3; CYPC1, cytP450 hydroxylase 97C1; BCH1/2, β-carotene hydroxylase 1/2; NXS, neoxanthin synthase; NCED, 9- cis -epoxycarotenoid dioxygenase; CCD, carotenoid cleavage dioxygenase; D27, β-carotene isomerase; CrtI, bacterial carotene desaturase; AGs, apocarotenoid glucosides; NFZ, norflurazon.

Article Snippet: Ohmiya A, Kishimoto S, Aida R, Yoshioka S, Sumitomo K. Carotenoid cleavage dioxygenase (CmCCD4a) contributes to white color formation in chrysanthemum petals .

Techniques: Mutagenesis