carbenicillin Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95
    Qiagen carbenicillin qiaprep spin miniprep kit
    Carbenicillin Qiaprep Spin Miniprep Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 95/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/carbenicillin qiaprep spin miniprep kit/product/Qiagen
    Average 95 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    carbenicillin qiaprep spin miniprep kit - by Bioz Stars, 2020-02
    95/100 stars
      Buy from Supplier

    95
    Millipore carbenicillin solution
    Killing of P. aeruginosa cells by <t>carbenicillin.</t> (A) Cells of logarithmic-phase, stationary-phase, and biofilm cultures were treated with carbenicillin for 6 h and then plated for colony counting. The data for biofilm cultures are plotted as CFU per peg of the biofilm device. The limit of detection is indicated by the solid horizontal line. The standard deviation for each point was calculated with n = 3. Biofilm, diamonds; stationary-phase cells, circles; logarithmic-phase cells, squares. (B) Data of panel A replotted as percent survival.
    Carbenicillin Solution, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/carbenicillin solution/product/Millipore
    Average 95 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    carbenicillin solution - by Bioz Stars, 2020-02
    95/100 stars
      Buy from Supplier

    95
    Millipore carbenicillin
    BfmRS controls antibiotic tolerance and persistence. 17978 (WT), EGA195 (Δ bfmS ), or EGA495 (Δ bfmRS ) bacteria grown to early-post exponential phase were challenged with the indicated antibiotic at the listed supra-MIC levels. Bacteria sampled at the indicated time points were washed, serially diluted, and plated on solid medium lacking antibiotics to determine viable bacterial counts at each time point (A, C, and E). Culture A 600 was also determined (B and D). In C and D, EGA495 (Δ bfmRS ) carrying no additional construct (-) or the indicated bfmRS allele in single copy were challenged with <t>carbenicillin</t> at 320 μg/ml. Data points represent mean ± s.d. (n ≥ 2).
    Carbenicillin, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 1626 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/carbenicillin/product/Millipore
    Average 95 stars, based on 1626 article reviews
    Price from $9.99 to $1999.99
    carbenicillin - by Bioz Stars, 2020-02
    95/100 stars
      Buy from Supplier

    94
    Duchefa carbenicillin
    BfmRS controls antibiotic tolerance and persistence. 17978 (WT), EGA195 (Δ bfmS ), or EGA495 (Δ bfmRS ) bacteria grown to early-post exponential phase were challenged with the indicated antibiotic at the listed supra-MIC levels. Bacteria sampled at the indicated time points were washed, serially diluted, and plated on solid medium lacking antibiotics to determine viable bacterial counts at each time point (A, C, and E). Culture A 600 was also determined (B and D). In C and D, EGA495 (Δ bfmRS ) carrying no additional construct (-) or the indicated bfmRS allele in single copy were challenged with <t>carbenicillin</t> at 320 μg/ml. Data points represent mean ± s.d. (n ≥ 2).
    Carbenicillin, supplied by Duchefa, used in various techniques. Bioz Stars score: 94/100, based on 141 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/carbenicillin/product/Duchefa
    Average 94 stars, based on 141 article reviews
    Price from $9.99 to $1999.99
    carbenicillin - by Bioz Stars, 2020-02
    94/100 stars
      Buy from Supplier

    95
    Fisher Scientific carbenicillin
    BfmRS controls antibiotic tolerance and persistence. 17978 (WT), EGA195 (Δ bfmS ), or EGA495 (Δ bfmRS ) bacteria grown to early-post exponential phase were challenged with the indicated antibiotic at the listed supra-MIC levels. Bacteria sampled at the indicated time points were washed, serially diluted, and plated on solid medium lacking antibiotics to determine viable bacterial counts at each time point (A, C, and E). Culture A 600 was also determined (B and D). In C and D, EGA495 (Δ bfmRS ) carrying no additional construct (-) or the indicated bfmRS allele in single copy were challenged with <t>carbenicillin</t> at 320 μg/ml. Data points represent mean ± s.d. (n ≥ 2).
    Carbenicillin, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 95/100, based on 120 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/carbenicillin/product/Fisher Scientific
    Average 95 stars, based on 120 article reviews
    Price from $9.99 to $1999.99
    carbenicillin - by Bioz Stars, 2020-02
    95/100 stars
      Buy from Supplier

    90
    Mediatech carbenicillin
    BfmRS controls antibiotic tolerance and persistence. 17978 (WT), EGA195 (Δ bfmS ), or EGA495 (Δ bfmRS ) bacteria grown to early-post exponential phase were challenged with the indicated antibiotic at the listed supra-MIC levels. Bacteria sampled at the indicated time points were washed, serially diluted, and plated on solid medium lacking antibiotics to determine viable bacterial counts at each time point (A, C, and E). Culture A 600 was also determined (B and D). In C and D, EGA495 (Δ bfmRS ) carrying no additional construct (-) or the indicated bfmRS allele in single copy were challenged with <t>carbenicillin</t> at 320 μg/ml. Data points represent mean ± s.d. (n ≥ 2).
    Carbenicillin, supplied by Mediatech, used in various techniques. Bioz Stars score: 90/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/carbenicillin/product/Mediatech
    Average 90 stars, based on 27 article reviews
    Price from $9.99 to $1999.99
    carbenicillin - by Bioz Stars, 2020-02
    90/100 stars
      Buy from Supplier

    99
    Thermo Fisher carbenicillin
    BfmRS controls antibiotic tolerance and persistence. 17978 (WT), EGA195 (Δ bfmS ), or EGA495 (Δ bfmRS ) bacteria grown to early-post exponential phase were challenged with the indicated antibiotic at the listed supra-MIC levels. Bacteria sampled at the indicated time points were washed, serially diluted, and plated on solid medium lacking antibiotics to determine viable bacterial counts at each time point (A, C, and E). Culture A 600 was also determined (B and D). In C and D, EGA495 (Δ bfmRS ) carrying no additional construct (-) or the indicated bfmRS allele in single copy were challenged with <t>carbenicillin</t> at 320 μg/ml. Data points represent mean ± s.d. (n ≥ 2).
    Carbenicillin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 420 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/carbenicillin/product/Thermo Fisher
    Average 99 stars, based on 420 article reviews
    Price from $9.99 to $1999.99
    carbenicillin - by Bioz Stars, 2020-02
    99/100 stars
      Buy from Supplier

    91
    Formedium carbenicillin
    BfmRS controls antibiotic tolerance and persistence. 17978 (WT), EGA195 (Δ bfmS ), or EGA495 (Δ bfmRS ) bacteria grown to early-post exponential phase were challenged with the indicated antibiotic at the listed supra-MIC levels. Bacteria sampled at the indicated time points were washed, serially diluted, and plated on solid medium lacking antibiotics to determine viable bacterial counts at each time point (A, C, and E). Culture A 600 was also determined (B and D). In C and D, EGA495 (Δ bfmRS ) carrying no additional construct (-) or the indicated bfmRS allele in single copy were challenged with <t>carbenicillin</t> at 320 μg/ml. Data points represent mean ± s.d. (n ≥ 2).
    Carbenicillin, supplied by Formedium, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/carbenicillin/product/Formedium
    Average 91 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    carbenicillin - by Bioz Stars, 2020-02
    91/100 stars
      Buy from Supplier

    94
    Gold Biotechnology Inc carbenicillin
    BfmRS controls antibiotic tolerance and persistence. 17978 (WT), EGA195 (Δ bfmS ), or EGA495 (Δ bfmRS ) bacteria grown to early-post exponential phase were challenged with the indicated antibiotic at the listed supra-MIC levels. Bacteria sampled at the indicated time points were washed, serially diluted, and plated on solid medium lacking antibiotics to determine viable bacterial counts at each time point (A, C, and E). Culture A 600 was also determined (B and D). In C and D, EGA495 (Δ bfmRS ) carrying no additional construct (-) or the indicated bfmRS allele in single copy were challenged with <t>carbenicillin</t> at 320 μg/ml. Data points represent mean ± s.d. (n ≥ 2).
    Carbenicillin, supplied by Gold Biotechnology Inc, used in various techniques. Bioz Stars score: 94/100, based on 47 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/carbenicillin/product/Gold Biotechnology Inc
    Average 94 stars, based on 47 article reviews
    Price from $9.99 to $1999.99
    carbenicillin - by Bioz Stars, 2020-02
    94/100 stars
      Buy from Supplier

    94
    Meridian Life Science carbenicillin
    BfmRS controls antibiotic tolerance and persistence. 17978 (WT), EGA195 (Δ bfmS ), or EGA495 (Δ bfmRS ) bacteria grown to early-post exponential phase were challenged with the indicated antibiotic at the listed supra-MIC levels. Bacteria sampled at the indicated time points were washed, serially diluted, and plated on solid medium lacking antibiotics to determine viable bacterial counts at each time point (A, C, and E). Culture A 600 was also determined (B and D). In C and D, EGA495 (Δ bfmRS ) carrying no additional construct (-) or the indicated bfmRS allele in single copy were challenged with <t>carbenicillin</t> at 320 μg/ml. Data points represent mean ± s.d. (n ≥ 2).
    Carbenicillin, supplied by Meridian Life Science, used in various techniques. Bioz Stars score: 94/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/carbenicillin/product/Meridian Life Science
    Average 94 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    carbenicillin - by Bioz Stars, 2020-02
    94/100 stars
      Buy from Supplier

    93
    Nacalai carbenicillin
    CreCNS is a DNA binding protein that compacts nucleoids in E. coli . ( A ) Coomassie Brilliant Blue-stained gel after SDS-PAGE of purified partial CreCNS and CreHLP proteins (80 pmol each). ( B ) Electrophoretic mobility shift assay of CreCNS and CreHLP proteins. The 100-bp DNA ladder was incubated with various amounts (80, 40, 20, and 10 pmol) of purified recombinant protein. ( C ) DH5α E. coli cells were transformed with the pQE80l vector containing the sequence of the CreCNS or CreHLP gene, or with the empty vector. Cells were grown in LB medium containing 50 µg/ml <t>carbenicillin</t> at 37 °C. At an OD600 of 0.4–0.6, CreCNS and CreHLP overexpression was induced with 1 mM IPTG for 4 h. The bacterial nucleoids were stained with DAPI. ( D ) Effect of nucleoid condensation mediated by CreCNS and CreHLP on DH5α E. coli cells. Escherichia coli cells were transformed with the pQE80l vector containing the sequence of the CreCNS or CreHLP gene, or with the empty vector. Cells were grown at 37 °C, and OD600 was measured 0–4 h after induction with 1 mM IPTG. Error bars represent standard deviation. There are three independent data points.
    Carbenicillin, supplied by Nacalai, used in various techniques. Bioz Stars score: 93/100, based on 47 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/carbenicillin/product/Nacalai
    Average 93 stars, based on 47 article reviews
    Price from $9.99 to $1999.99
    carbenicillin - by Bioz Stars, 2020-02
    93/100 stars
      Buy from Supplier

    94
    Research Products International carbenicillin
    CreCNS is a DNA binding protein that compacts nucleoids in E. coli . ( A ) Coomassie Brilliant Blue-stained gel after SDS-PAGE of purified partial CreCNS and CreHLP proteins (80 pmol each). ( B ) Electrophoretic mobility shift assay of CreCNS and CreHLP proteins. The 100-bp DNA ladder was incubated with various amounts (80, 40, 20, and 10 pmol) of purified recombinant protein. ( C ) DH5α E. coli cells were transformed with the pQE80l vector containing the sequence of the CreCNS or CreHLP gene, or with the empty vector. Cells were grown in LB medium containing 50 µg/ml <t>carbenicillin</t> at 37 °C. At an OD600 of 0.4–0.6, CreCNS and CreHLP overexpression was induced with 1 mM IPTG for 4 h. The bacterial nucleoids were stained with DAPI. ( D ) Effect of nucleoid condensation mediated by CreCNS and CreHLP on DH5α E. coli cells. Escherichia coli cells were transformed with the pQE80l vector containing the sequence of the CreCNS or CreHLP gene, or with the empty vector. Cells were grown at 37 °C, and OD600 was measured 0–4 h after induction with 1 mM IPTG. Error bars represent standard deviation. There are three independent data points.
    Carbenicillin, supplied by Research Products International, used in various techniques. Bioz Stars score: 94/100, based on 71 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/carbenicillin/product/Research Products International
    Average 94 stars, based on 71 article reviews
    Price from $9.99 to $1999.99
    carbenicillin - by Bioz Stars, 2020-02
    94/100 stars
      Buy from Supplier

    94
    Carl Roth GmbH carbenicillin
    Collateral sensitivity. Validated collateral sensitivity for 38 distinct populations obtained from Barbosa et al. (2017) . Six to seven populations resistant to CIP, ( a ); STR, ( b ); GEN, ( c ); PIT, ( d ); CAR, ( e ); and CEF, ( f ), were tested for collaterals sensitivity against 2 or four drugs, indicated above each of the panels. The ancestor PA14 is shown in black and the various populations in distinct colors. Points indicate averages of 3 technical replicates per population and drug concentration. CAR, <t>carbenicillin;</t> GEN, gentamicin; STR, streptomycin; PIT, piperacillin with tazobactam; CIP, ciprofloxacin; and CEF, cefsulodin; superscript R denotes resistance against the particular drug. The data for this figure is provided in Figure 5—figure supplement 1—source data 1 . Dose-response curves data of surviving populations in the generalized experiment. The data is shown in Figure 5—figure supplement 1 and summarized in Figure 5 . Optical density values were recorded after 12 hr of incubation at 37 °C.
    Carbenicillin, supplied by Carl Roth GmbH, used in various techniques. Bioz Stars score: 94/100, based on 74 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/carbenicillin/product/Carl Roth GmbH
    Average 94 stars, based on 74 article reviews
    Price from $9.99 to $1999.99
    carbenicillin - by Bioz Stars, 2020-02
    94/100 stars
      Buy from Supplier

    92
    Euromedex carbenicillin
    Collateral sensitivity. Validated collateral sensitivity for 38 distinct populations obtained from Barbosa et al. (2017) . Six to seven populations resistant to CIP, ( a ); STR, ( b ); GEN, ( c ); PIT, ( d ); CAR, ( e ); and CEF, ( f ), were tested for collaterals sensitivity against 2 or four drugs, indicated above each of the panels. The ancestor PA14 is shown in black and the various populations in distinct colors. Points indicate averages of 3 technical replicates per population and drug concentration. CAR, <t>carbenicillin;</t> GEN, gentamicin; STR, streptomycin; PIT, piperacillin with tazobactam; CIP, ciprofloxacin; and CEF, cefsulodin; superscript R denotes resistance against the particular drug. The data for this figure is provided in Figure 5—figure supplement 1—source data 1 . Dose-response curves data of surviving populations in the generalized experiment. The data is shown in Figure 5—figure supplement 1 and summarized in Figure 5 . Optical density values were recorded after 12 hr of incubation at 37 °C.
    Carbenicillin, supplied by Euromedex, used in various techniques. Bioz Stars score: 92/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/carbenicillin/product/Euromedex
    Average 92 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    carbenicillin - by Bioz Stars, 2020-02
    92/100 stars
      Buy from Supplier

    92
    Fisher Bioreagents carbenicillin
    Collateral sensitivity. Validated collateral sensitivity for 38 distinct populations obtained from Barbosa et al. (2017) . Six to seven populations resistant to CIP, ( a ); STR, ( b ); GEN, ( c ); PIT, ( d ); CAR, ( e ); and CEF, ( f ), were tested for collaterals sensitivity against 2 or four drugs, indicated above each of the panels. The ancestor PA14 is shown in black and the various populations in distinct colors. Points indicate averages of 3 technical replicates per population and drug concentration. CAR, <t>carbenicillin;</t> GEN, gentamicin; STR, streptomycin; PIT, piperacillin with tazobactam; CIP, ciprofloxacin; and CEF, cefsulodin; superscript R denotes resistance against the particular drug. The data for this figure is provided in Figure 5—figure supplement 1—source data 1 . Dose-response curves data of surviving populations in the generalized experiment. The data is shown in Figure 5—figure supplement 1 and summarized in Figure 5 . Optical density values were recorded after 12 hr of incubation at 37 °C.
    Carbenicillin, supplied by Fisher Bioreagents, used in various techniques. Bioz Stars score: 92/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/carbenicillin/product/Fisher Bioreagents
    Average 92 stars, based on 15 article reviews
    Price from $9.99 to $1999.99
    carbenicillin - by Bioz Stars, 2020-02
    92/100 stars
      Buy from Supplier

    95
    Teknova carbenicillin
    Collateral sensitivity. Validated collateral sensitivity for 38 distinct populations obtained from Barbosa et al. (2017) . Six to seven populations resistant to CIP, ( a ); STR, ( b ); GEN, ( c ); PIT, ( d ); CAR, ( e ); and CEF, ( f ), were tested for collaterals sensitivity against 2 or four drugs, indicated above each of the panels. The ancestor PA14 is shown in black and the various populations in distinct colors. Points indicate averages of 3 technical replicates per population and drug concentration. CAR, <t>carbenicillin;</t> GEN, gentamicin; STR, streptomycin; PIT, piperacillin with tazobactam; CIP, ciprofloxacin; and CEF, cefsulodin; superscript R denotes resistance against the particular drug. The data for this figure is provided in Figure 5—figure supplement 1—source data 1 . Dose-response curves data of surviving populations in the generalized experiment. The data is shown in Figure 5—figure supplement 1 and summarized in Figure 5 . Optical density values were recorded after 12 hr of incubation at 37 °C.
    Carbenicillin, supplied by Teknova, used in various techniques. Bioz Stars score: 95/100, based on 125 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/carbenicillin/product/Teknova
    Average 95 stars, based on 125 article reviews
    Price from $9.99 to $1999.99
    carbenicillin - by Bioz Stars, 2020-02
    95/100 stars
      Buy from Supplier

    90
    Gemini Bio carbenicillin
    Collateral sensitivity. Validated collateral sensitivity for 38 distinct populations obtained from Barbosa et al. (2017) . Six to seven populations resistant to CIP, ( a ); STR, ( b ); GEN, ( c ); PIT, ( d ); CAR, ( e ); and CEF, ( f ), were tested for collaterals sensitivity against 2 or four drugs, indicated above each of the panels. The ancestor PA14 is shown in black and the various populations in distinct colors. Points indicate averages of 3 technical replicates per population and drug concentration. CAR, <t>carbenicillin;</t> GEN, gentamicin; STR, streptomycin; PIT, piperacillin with tazobactam; CIP, ciprofloxacin; and CEF, cefsulodin; superscript R denotes resistance against the particular drug. The data for this figure is provided in Figure 5—figure supplement 1—source data 1 . Dose-response curves data of surviving populations in the generalized experiment. The data is shown in Figure 5—figure supplement 1 and summarized in Figure 5 . Optical density values were recorded after 12 hr of incubation at 37 °C.
    Carbenicillin, supplied by Gemini Bio, used in various techniques. Bioz Stars score: 90/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/carbenicillin/product/Gemini Bio
    Average 90 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    carbenicillin - by Bioz Stars, 2020-02
    90/100 stars
      Buy from Supplier

    94
    Applichem carbenicillin
    Collateral sensitivity. Validated collateral sensitivity for 38 distinct populations obtained from Barbosa et al. (2017) . Six to seven populations resistant to CIP, ( a ); STR, ( b ); GEN, ( c ); PIT, ( d ); CAR, ( e ); and CEF, ( f ), were tested for collaterals sensitivity against 2 or four drugs, indicated above each of the panels. The ancestor PA14 is shown in black and the various populations in distinct colors. Points indicate averages of 3 technical replicates per population and drug concentration. CAR, <t>carbenicillin;</t> GEN, gentamicin; STR, streptomycin; PIT, piperacillin with tazobactam; CIP, ciprofloxacin; and CEF, cefsulodin; superscript R denotes resistance against the particular drug. The data for this figure is provided in Figure 5—figure supplement 1—source data 1 . Dose-response curves data of surviving populations in the generalized experiment. The data is shown in Figure 5—figure supplement 1 and summarized in Figure 5 . Optical density values were recorded after 12 hr of incubation at 37 °C.
    Carbenicillin, supplied by Applichem, used in various techniques. Bioz Stars score: 94/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/carbenicillin/product/Applichem
    Average 94 stars, based on 25 article reviews
    Price from $9.99 to $1999.99
    carbenicillin - by Bioz Stars, 2020-02
    94/100 stars
      Buy from Supplier

    92
    IBI Scientific carbenicillin
    Collateral sensitivity. Validated collateral sensitivity for 38 distinct populations obtained from Barbosa et al. (2017) . Six to seven populations resistant to CIP, ( a ); STR, ( b ); GEN, ( c ); PIT, ( d ); CAR, ( e ); and CEF, ( f ), were tested for collaterals sensitivity against 2 or four drugs, indicated above each of the panels. The ancestor PA14 is shown in black and the various populations in distinct colors. Points indicate averages of 3 technical replicates per population and drug concentration. CAR, <t>carbenicillin;</t> GEN, gentamicin; STR, streptomycin; PIT, piperacillin with tazobactam; CIP, ciprofloxacin; and CEF, cefsulodin; superscript R denotes resistance against the particular drug. The data for this figure is provided in Figure 5—figure supplement 1—source data 1 . Dose-response curves data of surviving populations in the generalized experiment. The data is shown in Figure 5—figure supplement 1 and summarized in Figure 5 . Optical density values were recorded after 12 hr of incubation at 37 °C.
    Carbenicillin, supplied by IBI Scientific, used in various techniques. Bioz Stars score: 92/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/carbenicillin/product/IBI Scientific
    Average 92 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    carbenicillin - by Bioz Stars, 2020-02
    92/100 stars
      Buy from Supplier

    90
    Meiji Seika Pharma Co Ltd carbenicillin
    Collateral sensitivity. Validated collateral sensitivity for 38 distinct populations obtained from Barbosa et al. (2017) . Six to seven populations resistant to CIP, ( a ); STR, ( b ); GEN, ( c ); PIT, ( d ); CAR, ( e ); and CEF, ( f ), were tested for collaterals sensitivity against 2 or four drugs, indicated above each of the panels. The ancestor PA14 is shown in black and the various populations in distinct colors. Points indicate averages of 3 technical replicates per population and drug concentration. CAR, <t>carbenicillin;</t> GEN, gentamicin; STR, streptomycin; PIT, piperacillin with tazobactam; CIP, ciprofloxacin; and CEF, cefsulodin; superscript R denotes resistance against the particular drug. The data for this figure is provided in Figure 5—figure supplement 1—source data 1 . Dose-response curves data of surviving populations in the generalized experiment. The data is shown in Figure 5—figure supplement 1 and summarized in Figure 5 . Optical density values were recorded after 12 hr of incubation at 37 °C.
    Carbenicillin, supplied by Meiji Seika Pharma Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/carbenicillin/product/Meiji Seika Pharma Co Ltd
    Average 90 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    carbenicillin - by Bioz Stars, 2020-02
    90/100 stars
      Buy from Supplier

    90
    Melford Laboratories carbenicillin
    Collateral sensitivity. Validated collateral sensitivity for 38 distinct populations obtained from Barbosa et al. (2017) . Six to seven populations resistant to CIP, ( a ); STR, ( b ); GEN, ( c ); PIT, ( d ); CAR, ( e ); and CEF, ( f ), were tested for collaterals sensitivity against 2 or four drugs, indicated above each of the panels. The ancestor PA14 is shown in black and the various populations in distinct colors. Points indicate averages of 3 technical replicates per population and drug concentration. CAR, <t>carbenicillin;</t> GEN, gentamicin; STR, streptomycin; PIT, piperacillin with tazobactam; CIP, ciprofloxacin; and CEF, cefsulodin; superscript R denotes resistance against the particular drug. The data for this figure is provided in Figure 5—figure supplement 1—source data 1 . Dose-response curves data of surviving populations in the generalized experiment. The data is shown in Figure 5—figure supplement 1 and summarized in Figure 5 . Optical density values were recorded after 12 hr of incubation at 37 °C.
    Carbenicillin, supplied by Melford Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 54 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/carbenicillin/product/Melford Laboratories
    Average 90 stars, based on 54 article reviews
    Price from $9.99 to $1999.99
    carbenicillin - by Bioz Stars, 2020-02
    90/100 stars
      Buy from Supplier

    92
    Corning Life Sciences carbenicillin
    Collateral sensitivity. Validated collateral sensitivity for 38 distinct populations obtained from Barbosa et al. (2017) . Six to seven populations resistant to CIP, ( a ); STR, ( b ); GEN, ( c ); PIT, ( d ); CAR, ( e ); and CEF, ( f ), were tested for collaterals sensitivity against 2 or four drugs, indicated above each of the panels. The ancestor PA14 is shown in black and the various populations in distinct colors. Points indicate averages of 3 technical replicates per population and drug concentration. CAR, <t>carbenicillin;</t> GEN, gentamicin; STR, streptomycin; PIT, piperacillin with tazobactam; CIP, ciprofloxacin; and CEF, cefsulodin; superscript R denotes resistance against the particular drug. The data for this figure is provided in Figure 5—figure supplement 1—source data 1 . Dose-response curves data of surviving populations in the generalized experiment. The data is shown in Figure 5—figure supplement 1 and summarized in Figure 5 . Optical density values were recorded after 12 hr of incubation at 37 °C.
    Carbenicillin, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 92/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/carbenicillin/product/Corning Life Sciences
    Average 92 stars, based on 26 article reviews
    Price from $9.99 to $1999.99
    carbenicillin - by Bioz Stars, 2020-02
    92/100 stars
      Buy from Supplier

    90
    FUJIFILM carbenicillin
    Collateral sensitivity. Validated collateral sensitivity for 38 distinct populations obtained from Barbosa et al. (2017) . Six to seven populations resistant to CIP, ( a ); STR, ( b ); GEN, ( c ); PIT, ( d ); CAR, ( e ); and CEF, ( f ), were tested for collaterals sensitivity against 2 or four drugs, indicated above each of the panels. The ancestor PA14 is shown in black and the various populations in distinct colors. Points indicate averages of 3 technical replicates per population and drug concentration. CAR, <t>carbenicillin;</t> GEN, gentamicin; STR, streptomycin; PIT, piperacillin with tazobactam; CIP, ciprofloxacin; and CEF, cefsulodin; superscript R denotes resistance against the particular drug. The data for this figure is provided in Figure 5—figure supplement 1—source data 1 . Dose-response curves data of surviving populations in the generalized experiment. The data is shown in Figure 5—figure supplement 1 and summarized in Figure 5 . Optical density values were recorded after 12 hr of incubation at 37 °C.
    Carbenicillin, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 90/100, based on 57 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/carbenicillin/product/FUJIFILM
    Average 90 stars, based on 57 article reviews
    Price from $9.99 to $1999.99
    carbenicillin - by Bioz Stars, 2020-02
    90/100 stars
      Buy from Supplier

    94
    HiMedia Laboratories carbenicillin
    Collateral sensitivity. Validated collateral sensitivity for 38 distinct populations obtained from Barbosa et al. (2017) . Six to seven populations resistant to CIP, ( a ); STR, ( b ); GEN, ( c ); PIT, ( d ); CAR, ( e ); and CEF, ( f ), were tested for collaterals sensitivity against 2 or four drugs, indicated above each of the panels. The ancestor PA14 is shown in black and the various populations in distinct colors. Points indicate averages of 3 technical replicates per population and drug concentration. CAR, <t>carbenicillin;</t> GEN, gentamicin; STR, streptomycin; PIT, piperacillin with tazobactam; CIP, ciprofloxacin; and CEF, cefsulodin; superscript R denotes resistance against the particular drug. The data for this figure is provided in Figure 5—figure supplement 1—source data 1 . Dose-response curves data of surviving populations in the generalized experiment. The data is shown in Figure 5—figure supplement 1 and summarized in Figure 5 . Optical density values were recorded after 12 hr of incubation at 37 °C.
    Carbenicillin, supplied by HiMedia Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/carbenicillin/product/HiMedia Laboratories
    Average 94 stars, based on 32 article reviews
    Price from $9.99 to $1999.99
    carbenicillin - by Bioz Stars, 2020-02
    94/100 stars
      Buy from Supplier

    90
    BioShop carbenicillin
    Collateral sensitivity. Validated collateral sensitivity for 38 distinct populations obtained from Barbosa et al. (2017) . Six to seven populations resistant to CIP, ( a ); STR, ( b ); GEN, ( c ); PIT, ( d ); CAR, ( e ); and CEF, ( f ), were tested for collaterals sensitivity against 2 or four drugs, indicated above each of the panels. The ancestor PA14 is shown in black and the various populations in distinct colors. Points indicate averages of 3 technical replicates per population and drug concentration. CAR, <t>carbenicillin;</t> GEN, gentamicin; STR, streptomycin; PIT, piperacillin with tazobactam; CIP, ciprofloxacin; and CEF, cefsulodin; superscript R denotes resistance against the particular drug. The data for this figure is provided in Figure 5—figure supplement 1—source data 1 . Dose-response curves data of surviving populations in the generalized experiment. The data is shown in Figure 5—figure supplement 1 and summarized in Figure 5 . Optical density values were recorded after 12 hr of incubation at 37 °C.
    Carbenicillin, supplied by BioShop, used in various techniques. Bioz Stars score: 90/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/carbenicillin/product/BioShop
    Average 90 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    carbenicillin - by Bioz Stars, 2020-02
    90/100 stars
      Buy from Supplier

    90
    Cellgro carbenicillin
    Collateral sensitivity. Validated collateral sensitivity for 38 distinct populations obtained from Barbosa et al. (2017) . Six to seven populations resistant to CIP, ( a ); STR, ( b ); GEN, ( c ); PIT, ( d ); CAR, ( e ); and CEF, ( f ), were tested for collaterals sensitivity against 2 or four drugs, indicated above each of the panels. The ancestor PA14 is shown in black and the various populations in distinct colors. Points indicate averages of 3 technical replicates per population and drug concentration. CAR, <t>carbenicillin;</t> GEN, gentamicin; STR, streptomycin; PIT, piperacillin with tazobactam; CIP, ciprofloxacin; and CEF, cefsulodin; superscript R denotes resistance against the particular drug. The data for this figure is provided in Figure 5—figure supplement 1—source data 1 . Dose-response curves data of surviving populations in the generalized experiment. The data is shown in Figure 5—figure supplement 1 and summarized in Figure 5 . Optical density values were recorded after 12 hr of incubation at 37 °C.
    Carbenicillin, supplied by Cellgro, used in various techniques. Bioz Stars score: 90/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/carbenicillin/product/Cellgro
    Average 90 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    carbenicillin - by Bioz Stars, 2020-02
    90/100 stars
      Buy from Supplier

    90
    Chem Impex International carbenicillin
    Collateral sensitivity. Validated collateral sensitivity for 38 distinct populations obtained from Barbosa et al. (2017) . Six to seven populations resistant to CIP, ( a ); STR, ( b ); GEN, ( c ); PIT, ( d ); CAR, ( e ); and CEF, ( f ), were tested for collaterals sensitivity against 2 or four drugs, indicated above each of the panels. The ancestor PA14 is shown in black and the various populations in distinct colors. Points indicate averages of 3 technical replicates per population and drug concentration. CAR, <t>carbenicillin;</t> GEN, gentamicin; STR, streptomycin; PIT, piperacillin with tazobactam; CIP, ciprofloxacin; and CEF, cefsulodin; superscript R denotes resistance against the particular drug. The data for this figure is provided in Figure 5—figure supplement 1—source data 1 . Dose-response curves data of surviving populations in the generalized experiment. The data is shown in Figure 5—figure supplement 1 and summarized in Figure 5 . Optical density values were recorded after 12 hr of incubation at 37 °C.
    Carbenicillin, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/carbenicillin/product/Chem Impex International
    Average 90 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    carbenicillin - by Bioz Stars, 2020-02
    90/100 stars
      Buy from Supplier

    90
    Omega Scientific Inc carbenicillin
    Collateral sensitivity. Validated collateral sensitivity for 38 distinct populations obtained from Barbosa et al. (2017) . Six to seven populations resistant to CIP, ( a ); STR, ( b ); GEN, ( c ); PIT, ( d ); CAR, ( e ); and CEF, ( f ), were tested for collaterals sensitivity against 2 or four drugs, indicated above each of the panels. The ancestor PA14 is shown in black and the various populations in distinct colors. Points indicate averages of 3 technical replicates per population and drug concentration. CAR, <t>carbenicillin;</t> GEN, gentamicin; STR, streptomycin; PIT, piperacillin with tazobactam; CIP, ciprofloxacin; and CEF, cefsulodin; superscript R denotes resistance against the particular drug. The data for this figure is provided in Figure 5—figure supplement 1—source data 1 . Dose-response curves data of surviving populations in the generalized experiment. The data is shown in Figure 5—figure supplement 1 and summarized in Figure 5 . Optical density values were recorded after 12 hr of incubation at 37 °C.
    Carbenicillin, supplied by Omega Scientific Inc, used in various techniques. Bioz Stars score: 90/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/carbenicillin/product/Omega Scientific Inc
    Average 90 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    carbenicillin - by Bioz Stars, 2020-02
    90/100 stars
      Buy from Supplier

    90
    Thermo Fisher carbenicillin disodium salt
    Impact of sugar metabolites on E. coli antibiotic sensitivity. (a) MG1655 WT was treated with 30 μg <t>carbenicillin</t> ml − 1 , 3 μg gentamicin ml − 1 or 0.125 μg ciprofloxacin ml
    Carbenicillin Disodium Salt, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/carbenicillin disodium salt/product/Thermo Fisher
    Average 90 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    carbenicillin disodium salt - by Bioz Stars, 2020-02
    90/100 stars
      Buy from Supplier

    76
    FUJIFILM carbenicillin sodium
    Impact of sugar metabolites on E. coli antibiotic sensitivity. (a) MG1655 WT was treated with 30 μg <t>carbenicillin</t> ml − 1 , 3 μg gentamicin ml − 1 or 0.125 μg ciprofloxacin ml
    Carbenicillin Sodium, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 76/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/carbenicillin sodium/product/FUJIFILM
    Average 76 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    carbenicillin sodium - by Bioz Stars, 2020-02
    76/100 stars
      Buy from Supplier

    Image Search Results


    Killing of P. aeruginosa cells by carbenicillin. (A) Cells of logarithmic-phase, stationary-phase, and biofilm cultures were treated with carbenicillin for 6 h and then plated for colony counting. The data for biofilm cultures are plotted as CFU per peg of the biofilm device. The limit of detection is indicated by the solid horizontal line. The standard deviation for each point was calculated with n = 3. Biofilm, diamonds; stationary-phase cells, circles; logarithmic-phase cells, squares. (B) Data of panel A replotted as percent survival.

    Journal: Journal of Bacteriology

    Article Title: Biofilms and Planktonic Cells of Pseudomonas aeruginosa Have Similar Resistance to Killing by Antimicrobials

    doi: 10.1128/JB.183.23.6746-6751.2001

    Figure Lengend Snippet: Killing of P. aeruginosa cells by carbenicillin. (A) Cells of logarithmic-phase, stationary-phase, and biofilm cultures were treated with carbenicillin for 6 h and then plated for colony counting. The data for biofilm cultures are plotted as CFU per peg of the biofilm device. The limit of detection is indicated by the solid horizontal line. The standard deviation for each point was calculated with n = 3. Biofilm, diamonds; stationary-phase cells, circles; logarithmic-phase cells, squares. (B) Data of panel A replotted as percent survival.

    Article Snippet: Tobramycin and carbenicillin were obtained from Sigma.

    Techniques: Standard Deviation

    BfmRS controls antibiotic tolerance and persistence. 17978 (WT), EGA195 (Δ bfmS ), or EGA495 (Δ bfmRS ) bacteria grown to early-post exponential phase were challenged with the indicated antibiotic at the listed supra-MIC levels. Bacteria sampled at the indicated time points were washed, serially diluted, and plated on solid medium lacking antibiotics to determine viable bacterial counts at each time point (A, C, and E). Culture A 600 was also determined (B and D). In C and D, EGA495 (Δ bfmRS ) carrying no additional construct (-) or the indicated bfmRS allele in single copy were challenged with carbenicillin at 320 μg/ml. Data points represent mean ± s.d. (n ≥ 2).

    Journal: PLoS Pathogens

    Article Title: A global regulatory system links virulence and antibiotic resistance to envelope homeostasis in Acinetobacter baumannii

    doi: 10.1371/journal.ppat.1007030

    Figure Lengend Snippet: BfmRS controls antibiotic tolerance and persistence. 17978 (WT), EGA195 (Δ bfmS ), or EGA495 (Δ bfmRS ) bacteria grown to early-post exponential phase were challenged with the indicated antibiotic at the listed supra-MIC levels. Bacteria sampled at the indicated time points were washed, serially diluted, and plated on solid medium lacking antibiotics to determine viable bacterial counts at each time point (A, C, and E). Culture A 600 was also determined (B and D). In C and D, EGA495 (Δ bfmRS ) carrying no additional construct (-) or the indicated bfmRS allele in single copy were challenged with carbenicillin at 320 μg/ml. Data points represent mean ± s.d. (n ≥ 2).

    Article Snippet: Gentamicin (Gm, 15 μg/ml), kanamycin (Km, 10 μg/ml), carbenicillin (Cb, 100 μg/ml), and/or tetracycline (Tc, 10 μg/ml) (Sigma) were used at noted concentration in solid medium.

    Techniques: Construct

    CreCNS is a DNA binding protein that compacts nucleoids in E. coli . ( A ) Coomassie Brilliant Blue-stained gel after SDS-PAGE of purified partial CreCNS and CreHLP proteins (80 pmol each). ( B ) Electrophoretic mobility shift assay of CreCNS and CreHLP proteins. The 100-bp DNA ladder was incubated with various amounts (80, 40, 20, and 10 pmol) of purified recombinant protein. ( C ) DH5α E. coli cells were transformed with the pQE80l vector containing the sequence of the CreCNS or CreHLP gene, or with the empty vector. Cells were grown in LB medium containing 50 µg/ml carbenicillin at 37 °C. At an OD600 of 0.4–0.6, CreCNS and CreHLP overexpression was induced with 1 mM IPTG for 4 h. The bacterial nucleoids were stained with DAPI. ( D ) Effect of nucleoid condensation mediated by CreCNS and CreHLP on DH5α E. coli cells. Escherichia coli cells were transformed with the pQE80l vector containing the sequence of the CreCNS or CreHLP gene, or with the empty vector. Cells were grown at 37 °C, and OD600 was measured 0–4 h after induction with 1 mM IPTG. Error bars represent standard deviation. There are three independent data points.

    Journal: Genome Biology and Evolution

    Article Title: Eukaryotic Components Remodeled Chloroplast Nucleoid Organization during the Green Plant Evolution

    doi: 10.1093/gbe/evv233

    Figure Lengend Snippet: CreCNS is a DNA binding protein that compacts nucleoids in E. coli . ( A ) Coomassie Brilliant Blue-stained gel after SDS-PAGE of purified partial CreCNS and CreHLP proteins (80 pmol each). ( B ) Electrophoretic mobility shift assay of CreCNS and CreHLP proteins. The 100-bp DNA ladder was incubated with various amounts (80, 40, 20, and 10 pmol) of purified recombinant protein. ( C ) DH5α E. coli cells were transformed with the pQE80l vector containing the sequence of the CreCNS or CreHLP gene, or with the empty vector. Cells were grown in LB medium containing 50 µg/ml carbenicillin at 37 °C. At an OD600 of 0.4–0.6, CreCNS and CreHLP overexpression was induced with 1 mM IPTG for 4 h. The bacterial nucleoids were stained with DAPI. ( D ) Effect of nucleoid condensation mediated by CreCNS and CreHLP on DH5α E. coli cells. Escherichia coli cells were transformed with the pQE80l vector containing the sequence of the CreCNS or CreHLP gene, or with the empty vector. Cells were grown at 37 °C, and OD600 was measured 0–4 h after induction with 1 mM IPTG. Error bars represent standard deviation. There are three independent data points.

    Article Snippet: Antibody Preparation pQE80l (Qiagen, Venlo, Netherlands) vectors harboring the cDNA sequences encoding CreCNS, CreHLP, CreWhirly, CreSWIB2, KfHLP, KfpTAC3, KfWhirly and KfSWIB were prepared and transformed into the E scherichia coli BL21 strain and selected on Luria–Bertani (LB) agar medium containing 50 µg/ml carbenicillin (Nacalai Tesque).

    Techniques: Binding Assay, Staining, SDS Page, Purification, Electrophoretic Mobility Shift Assay, Incubation, Recombinant, Transformation Assay, Plasmid Preparation, Sequencing, Over Expression, Standard Deviation

    Collateral sensitivity. Validated collateral sensitivity for 38 distinct populations obtained from Barbosa et al. (2017) . Six to seven populations resistant to CIP, ( a ); STR, ( b ); GEN, ( c ); PIT, ( d ); CAR, ( e ); and CEF, ( f ), were tested for collaterals sensitivity against 2 or four drugs, indicated above each of the panels. The ancestor PA14 is shown in black and the various populations in distinct colors. Points indicate averages of 3 technical replicates per population and drug concentration. CAR, carbenicillin; GEN, gentamicin; STR, streptomycin; PIT, piperacillin with tazobactam; CIP, ciprofloxacin; and CEF, cefsulodin; superscript R denotes resistance against the particular drug. The data for this figure is provided in Figure 5—figure supplement 1—source data 1 . Dose-response curves data of surviving populations in the generalized experiment. The data is shown in Figure 5—figure supplement 1 and summarized in Figure 5 . Optical density values were recorded after 12 hr of incubation at 37 °C.

    Journal: eLife

    Article Title: Evolutionary stability of collateral sensitivity to antibiotics in the model pathogen Pseudomonas aeruginosa

    doi: 10.7554/eLife.51481

    Figure Lengend Snippet: Collateral sensitivity. Validated collateral sensitivity for 38 distinct populations obtained from Barbosa et al. (2017) . Six to seven populations resistant to CIP, ( a ); STR, ( b ); GEN, ( c ); PIT, ( d ); CAR, ( e ); and CEF, ( f ), were tested for collaterals sensitivity against 2 or four drugs, indicated above each of the panels. The ancestor PA14 is shown in black and the various populations in distinct colors. Points indicate averages of 3 technical replicates per population and drug concentration. CAR, carbenicillin; GEN, gentamicin; STR, streptomycin; PIT, piperacillin with tazobactam; CIP, ciprofloxacin; and CEF, cefsulodin; superscript R denotes resistance against the particular drug. The data for this figure is provided in Figure 5—figure supplement 1—source data 1 . Dose-response curves data of surviving populations in the generalized experiment. The data is shown in Figure 5—figure supplement 1 and summarized in Figure 5 . Optical density values were recorded after 12 hr of incubation at 37 °C.

    Article Snippet: The resistant populations were previously selected for high levels of resistance against protein synthesis inhibitors from the aminoglycoside family, gentamicin (GEN; Carl Roth, Germany; Ref. HN09.1) and streptomycin (STR; Sigma-Aldrich, USA; Ref. S6501-5G), or alternatively cell-wall synthesis inhibitors from the β-lactam family, carbenicillin (CAR; Carl Roth, Germany; Ref. 6344.2) and piperacillin/tazobactam (PIT; Sigma-Aldrich, USA; Refs. P8396-1G and T2820-10MG).

    Techniques: Concentration Assay, Incubation

    Reciprocal collateral sensitivity and experimental design. ( a ) Two-step experimental evolution: resistant populations of P. aeruginosa PA14 were previously experimentally evolved ( Barbosa et al., 2017 ) with increasing concentrations of a particular drug (here labeled A), resulting in bacteria becoming hypersensitive to other drugs (here labeled B). In a second step, A-resistant clones were experimentally evolved in the presence of drug B, using four selection regimes: (i) strong dose increase of drug B in the presence of a constant high dose of drug A; (ii) mild dose increase of B in the presence of A; (iii) strong dose increases of B in the absence of A; and (iv) mild dose increase of B in the absence of A. Concentrations of B were increased using linear ramps starting at IC 50 (dashed black lines) and ending at levels above the IC 95 of the collaterally sensitive clone (mild increases, dashed orange line), or that of the PA14 wild type strain (strong increases, dashed black lines; detailed information on concentrations in Supplementary Table 1). ( b ) Validated reciprocity of collateral sensitivity for the isolated resistant clones and drug pair PIT/STR, and ( c ) CAR/GEN. Mean ±CI95, n = 8. Vertical dashed lines indicate the starting (black) and final doses of the mild (light orange) and strong drug increases (light blue). CAR, carbenicillin; GEN, gentamicin; STR, streptomycin; PIT, piperacillin with tazobactam; superscript R denotes resistance against the particular drug. The following supplementary table and source data are available for Figure 1b and c : Supplementary file 1 -Figure 1-supplementary table 1 and Figure 1—source data 1 . Mean optical density and CI95 values obtained after 12 hr of growth in minimal media and different antibiotics as reported in Figure 1 . The populations tested here include the PA14 wild type, and four resistant populations. Each value is the average of 8 technical replicates per bacterial population.

    Journal: eLife

    Article Title: Evolutionary stability of collateral sensitivity to antibiotics in the model pathogen Pseudomonas aeruginosa

    doi: 10.7554/eLife.51481

    Figure Lengend Snippet: Reciprocal collateral sensitivity and experimental design. ( a ) Two-step experimental evolution: resistant populations of P. aeruginosa PA14 were previously experimentally evolved ( Barbosa et al., 2017 ) with increasing concentrations of a particular drug (here labeled A), resulting in bacteria becoming hypersensitive to other drugs (here labeled B). In a second step, A-resistant clones were experimentally evolved in the presence of drug B, using four selection regimes: (i) strong dose increase of drug B in the presence of a constant high dose of drug A; (ii) mild dose increase of B in the presence of A; (iii) strong dose increases of B in the absence of A; and (iv) mild dose increase of B in the absence of A. Concentrations of B were increased using linear ramps starting at IC 50 (dashed black lines) and ending at levels above the IC 95 of the collaterally sensitive clone (mild increases, dashed orange line), or that of the PA14 wild type strain (strong increases, dashed black lines; detailed information on concentrations in Supplementary Table 1). ( b ) Validated reciprocity of collateral sensitivity for the isolated resistant clones and drug pair PIT/STR, and ( c ) CAR/GEN. Mean ±CI95, n = 8. Vertical dashed lines indicate the starting (black) and final doses of the mild (light orange) and strong drug increases (light blue). CAR, carbenicillin; GEN, gentamicin; STR, streptomycin; PIT, piperacillin with tazobactam; superscript R denotes resistance against the particular drug. The following supplementary table and source data are available for Figure 1b and c : Supplementary file 1 -Figure 1-supplementary table 1 and Figure 1—source data 1 . Mean optical density and CI95 values obtained after 12 hr of growth in minimal media and different antibiotics as reported in Figure 1 . The populations tested here include the PA14 wild type, and four resistant populations. Each value is the average of 8 technical replicates per bacterial population.

    Article Snippet: The resistant populations were previously selected for high levels of resistance against protein synthesis inhibitors from the aminoglycoside family, gentamicin (GEN; Carl Roth, Germany; Ref. HN09.1) and streptomycin (STR; Sigma-Aldrich, USA; Ref. S6501-5G), or alternatively cell-wall synthesis inhibitors from the β-lactam family, carbenicillin (CAR; Carl Roth, Germany; Ref. 6344.2) and piperacillin/tazobactam (PIT; Sigma-Aldrich, USA; Refs. P8396-1G and T2820-10MG).

    Techniques: Labeling, Clone Assay, Selection, Isolation

    Re-sensitization to gentamicin (GEN) upon adaptation to carbenicillin (CAR). We calculated ( a ) dose-response relationships against GEN of 15 populations adapted to strong (n = 7, light blue) and mild (n = 8, light orange) drug increases compared to the PA14 ancestor (black, bottom-right panel). Mean ±CI95, n = 3 technical replicates. In most cases, the evolved population had the same MIC as PA14. Two populations (aF7 and aG7) showed lower MICs than PA14, while two (aH8 and bH8) showed slightly higher ones. The labels within each graph correspond to the code used during experimental evolution. Data from Source Data 4 ( b ) Difference in the area under the curve (AUC) between each evaluated population and the PA14 ancestor. Scaling of the y-axis is equivalent to Figure 3d . None of the populations was significantly different from the ancestor (Wilcoxon’s test, n = 3, adjusted P values min > 0.4, and max

    Journal: eLife

    Article Title: Evolutionary stability of collateral sensitivity to antibiotics in the model pathogen Pseudomonas aeruginosa

    doi: 10.7554/eLife.51481

    Figure Lengend Snippet: Re-sensitization to gentamicin (GEN) upon adaptation to carbenicillin (CAR). We calculated ( a ) dose-response relationships against GEN of 15 populations adapted to strong (n = 7, light blue) and mild (n = 8, light orange) drug increases compared to the PA14 ancestor (black, bottom-right panel). Mean ±CI95, n = 3 technical replicates. In most cases, the evolved population had the same MIC as PA14. Two populations (aF7 and aG7) showed lower MICs than PA14, while two (aH8 and bH8) showed slightly higher ones. The labels within each graph correspond to the code used during experimental evolution. Data from Source Data 4 ( b ) Difference in the area under the curve (AUC) between each evaluated population and the PA14 ancestor. Scaling of the y-axis is equivalent to Figure 3d . None of the populations was significantly different from the ancestor (Wilcoxon’s test, n = 3, adjusted P values min > 0.4, and max

    Article Snippet: The resistant populations were previously selected for high levels of resistance against protein synthesis inhibitors from the aminoglycoside family, gentamicin (GEN; Carl Roth, Germany; Ref. HN09.1) and streptomycin (STR; Sigma-Aldrich, USA; Ref. S6501-5G), or alternatively cell-wall synthesis inhibitors from the β-lactam family, carbenicillin (CAR; Carl Roth, Germany; Ref. 6344.2) and piperacillin/tazobactam (PIT; Sigma-Aldrich, USA; Refs. P8396-1G and T2820-10MG).

    Techniques:

    Impact of sugar metabolites on E. coli antibiotic sensitivity. (a) MG1655 WT was treated with 30 μg carbenicillin ml − 1 , 3 μg gentamicin ml − 1 or 0.125 μg ciprofloxacin ml

    Journal: Microbiology

    Article Title: The bactericidal activity of β-lactam antibiotics is increased by metabolizable sugar species

    doi: 10.1099/mic.0.000152

    Figure Lengend Snippet: Impact of sugar metabolites on E. coli antibiotic sensitivity. (a) MG1655 WT was treated with 30 μg carbenicillin ml − 1 , 3 μg gentamicin ml − 1 or 0.125 μg ciprofloxacin ml

    Article Snippet: Ciprofloxacin, gentamicin, cefuroxime and the tested carbon sources were purchased from Sigma-Aldrich, and carbenicillin disodium salt was purchased from Invitrogen.

    Techniques:

    Filament formation and lysis of E. coli MG1655 WT (a) and mutants deficient in arabinose metabolism (Δ araC ) (b) or glucose metabolism (Δ glc ) (c) were treated with carbenicillin alone or in combination with 12 mM l -arabinose or

    Journal: Microbiology

    Article Title: The bactericidal activity of β-lactam antibiotics is increased by metabolizable sugar species

    doi: 10.1099/mic.0.000152

    Figure Lengend Snippet: Filament formation and lysis of E. coli MG1655 WT (a) and mutants deficient in arabinose metabolism (Δ araC ) (b) or glucose metabolism (Δ glc ) (c) were treated with carbenicillin alone or in combination with 12 mM l -arabinose or

    Article Snippet: Ciprofloxacin, gentamicin, cefuroxime and the tested carbon sources were purchased from Sigma-Aldrich, and carbenicillin disodium salt was purchased from Invitrogen.

    Techniques: Lysis, Gas Chromatography

    β-Lactam sensitivity of a (p)ppGpp 0 strain is unaffected by sugar. (a) Bacterial cell survival was determined after 18 h of treatment without (black bars) or with 30 μg carbenicillin ml − 1 alone (chequered

    Journal: Microbiology

    Article Title: The bactericidal activity of β-lactam antibiotics is increased by metabolizable sugar species

    doi: 10.1099/mic.0.000152

    Figure Lengend Snippet: β-Lactam sensitivity of a (p)ppGpp 0 strain is unaffected by sugar. (a) Bacterial cell survival was determined after 18 h of treatment without (black bars) or with 30 μg carbenicillin ml − 1 alone (chequered

    Article Snippet: Ciprofloxacin, gentamicin, cefuroxime and the tested carbon sources were purchased from Sigma-Aldrich, and carbenicillin disodium salt was purchased from Invitrogen.

    Techniques: