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ATCC
caption a7 parental yeast strains genotype parent plasmid reference 972 wild type atcc Caption A7 Parental Yeast Strains Genotype Parent Plasmid Reference 972 Wild Type Atcc, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/product/caption+a7+stem/pmc04041700-246-22-33?v=ATCC Average 99 stars, based on 1 article reviews
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ATCC
caption a7 culture vtec reference escherichia coli h19 vt1 escherichia coli e32511  Caption A7 Culture Vtec Reference Escherichia Coli H19 Vt1 Escherichia Coli E32511, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/product/caption+a7+stem/pmc00124685-161-19-52?v=ATCC Average 93 stars, based on 1 article reviews
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Bio-Rad
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CHROMagar - DRG
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ATCC
caption a7 multi step resistance selection Caption A7 Multi Step Resistance Selection, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/product/caption+a7+stem/pmc05437844-129-39-65?v=ATCC Average 96 stars, based on 1 article reviews
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Thermo Fisher
gene exp gria1 rn00709588 m1  Gene Exp Gria1 Rn00709588 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/product/caption+a7+stem/pmc06505688-139-38--1?v=Thermo+Fisher Average 98 stars, based on 1 article reviews
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Gatan Inc
caption a7 stem ![<t>STEM-EELS</t> imaging of frozen hydrated specimens at a beam energy of 100 keV using a Gatan PEELS interfaced to a VG Microscopes HB501 STEM. (a) Low-loss spectra up to an energy loss of 30 eV from major chemical constituents of cells; these can be used to fit spectra from cryosectioned cells to give quantitative compositional information; (b) Low-dose dark-field STEM of frozen hydrated liver cryosection showing no contrast apart from deformation lines; scale bar = 1 μm; (c) Water map of hepatocytes generated by multiple least squares fitting of water and protein reference spectra at each pixel revealing: mitochondria (M), cytoplasm (C), red blood cells (R), plasma (P) and lipid droplets (L); (d) Water content histogram for 2700 pixels of cytoplasm (light bars) and 500 pixels of mitochondria (dark bars) in hepatocyte, showing approximately Gaussian peaks with half width ~5%. From S. Sun et al. [27].](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_8483/pmc05468483/pmc05468483__nihms846160f4.jpg) Caption A7 Stem, supplied by Gatan Inc, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/product/caption+a7+stem/pmc05468483-72-30-47?v=Gatan+Inc Average 98 stars, based on 1 article reviews
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ATCC
t5 caption a7 parent parent mcr 1 a baumannii atcc  T5 Caption A7 Parent Parent Mcr 1 A Baumannii Atcc, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/product/caption+a7+stem/pmc05632226-72-43-52?v=ATCC Average 99 stars, based on 1 article reviews
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Bio-Rad
t5 caption a7 purification step total protein mg activity T5 Caption A7 Purification Step Total Protein Mg Activity, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/product/caption+a7+stem/pmc05655811-261-132-83?v=Bio-Rad Average 93 stars, based on 1 article reviews
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ATCC
caption a7 strain parent strain relevant genotype main glycan modification  Caption A7 Strain Parent Strain Relevant Genotype Main Glycan Modification, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/product/caption+a7+stem/pmc02876500-202-27-46?v=ATCC Average 94 stars, based on 1 article reviews
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ATCC
caption a7 survival curves Caption A7 Survival Curves, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/product/caption+a7+stem/pmc03416510-338-32-49?v=ATCC Average 99 stars, based on 1 article reviews
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Gatan Inc
caption a7 a chemical map Caption A7 A Chemical Map, supplied by Gatan Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/product/caption+a7+stem/pmc00139182-33-24-57?v=Gatan+Inc Average 96 stars, based on 1 article reviews
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Image Search Results
Journal:
Article Title: Detection of Verotoxigenic Escherichia coli by Magnetic Capture-Hybridization PCR
doi:
Figure Lengend Snippet: Bacterial cultures used in this study
Article Snippet: The bacterial cultures used in this study are detailed in Table . table ft1 table-wrap mode="anchored" t5 TABLE 1
Techniques:
Journal:
Article Title: Detection of Verotoxigenic Escherichia coli by Magnetic Capture-Hybridization PCR
doi:
Figure Lengend Snippet: Information on capture probes for VT1 and VT2
Article Snippet: The bacterial cultures used in this study are detailed in Table . table ft1 table-wrap mode="anchored" t5 TABLE 1
Techniques:
Journal:
Article Title: Detection of Verotoxigenic Escherichia coli by Magnetic Capture-Hybridization PCR
doi:
Figure Lengend Snippet: Specificity of capture probes as determined by Southern hybridization of PCR-amplified products from VTEC reference cultures with Dig-dUTP-labeled capture probes. Conventional PCR products were amplified from total cellular DNA of E. coli H19 (VT1), E32511 (VT2), 933W (VT2), 412 (VTe), and H.I.8 (VT2vha). After being separated by electrophoresis, the amplified products were transferred to a positively charged nylon membrane and hybridized with Dig-dUTP-labeled VT2 capture probe. Formed hybrids were detected with the Bioluminescence Detection Kit from Boehringer Mannheim. Next the probe was washed off and then rehybridized with Dig-dUTP-labeled VT1 capture probe. (A) Agarose gel containing amplified PCR products. (B) Southern hybridization with capture probe of VT2. (C) Southern hybridization with capture probe of VT1. Lanes: a, 1-kb DNA ladder; b, E. coli H19; c, E. coli E32511; d, E. coli 933W; e, E. coli 412; f, E. coli H.I.8.
Article Snippet: The bacterial cultures used in this study are detailed in Table . table ft1 table-wrap mode="anchored" t5 TABLE 1
Techniques: Hybridization, Amplification, Labeling, Electrophoresis, Agarose Gel Electrophoresis
Journal:
Article Title: Detection of Verotoxigenic Escherichia coli by Magnetic Capture-Hybridization PCR
doi:
Figure Lengend Snippet: Specificity of capture probes as determined by slot hybridization of total cellular DNA of 5 VTEC reference and 13 negative control cultures with Dig-dUTP-labeled capture probes. (a) Hybridization with VT1 capture probe. (b) Hybridization with VT2 capture probe. The DNA samples on each slot were as follows: A1, Salmonella typhimurium; A2, Salmonella heidelberg; A3, Salmonella hadar; A4, Salmonella enteritidis; A5, Salmonella infantis; A6, Shigella dysenteriae; B1, Aeromonas sobria; B2, Enterobacter aerogenes; B3, Serratia marcescens; B4, Klebsiella pneumoniae; B5, Escherichia coli ATCC 10789; B6, Proteus vulgaris; C1, Yersinia enterocolitica; C2 to C6, blank; D1, E. coli H19 (VT1); D2, E. coli E32511 (VT2); D3, E. coli 933W (VT2); D4, E. coli 412 (VTe); D5, E. coli H.I.8 (VT2vha); D6, blank.
Article Snippet: The bacterial cultures used in this study are detailed in Table . table ft1 table-wrap mode="anchored" t5 TABLE 1
Techniques: Hybridization, Negative Control, Labeling
Journal:
Article Title: Detection of Verotoxigenic Escherichia coli by Magnetic Capture-Hybridization PCR
doi:
Figure Lengend Snippet: MCH-PCR products amplified from different VT-producing E. coli O157:H7 isolates. Lanes: a and f, 1-kb DNA ladder; b, MCH-PCR products amplified from E. coli O157:H7 920321, which produced both VT1 and VT2; c, MCH-PCR product from E. coli O157:H7 920160, a VT1 producer; d, MCH-PCR products amplified from E. coli O157:H7 920191, a VT2 producer; e, negative control.
Article Snippet: The bacterial cultures used in this study are detailed in Table . table ft1 table-wrap mode="anchored" t5 TABLE 1
Techniques: Amplification, Produced, Negative Control
Journal:
Article Title: Detection of Verotoxigenic Escherichia coli by Magnetic Capture-Hybridization PCR
doi:
Figure Lengend Snippet: Detection of VTEC by MCH-PCR and correlation between MCH-PCR and VT ELISA results. (A) PCR products amplified by MCH-PCR. (B) Results of VT ELISA. Amplified products were as follows (by lane): a, E. coli O115:H18; b, E. coli O121:H7; c, E. coli O157:H7 930086; d, E. coli O157:H7 920333; e, E. coli O157:H7 phage type (PT) 23; f, E. coli O157:H7 PT30; g, E. coli O157:H7 PT34; h, E. coli O157:H7 PT23; i, E. coli O157:H7 920027; j, E. coli O6:H34; k, E. coli O103:H2; l, E. coli O157:H7 PT14; m, 1-kb DNA ladder.
Article Snippet: The bacterial cultures used in this study are detailed in Table . table ft1 table-wrap mode="anchored" t5 TABLE 1
Techniques: Enzyme-linked Immunosorbent Assay, Amplification
Journal:
Article Title: Detection of Verotoxigenic Escherichia coli by Magnetic Capture-Hybridization PCR
doi:
Figure Lengend Snippet: Detection of VTEC from artificially contaminated ground beef. Samples of 25 g of ground beef were contaminated with E. coli O157:H7 920321 at a rate of 103 to 100 CFU/g. The contaminated meat samples were preenriched in 225 ml of BHI broth at 37°C for 15 h. A volume of 1 ml was taken from each sample and used in MCH-PCR amplification. Lanes: a and g, 1-kb DNA ladder; b to e, PCR products amplified from ground beef initially contaminated with 103, 102, 101, and 100 CFU of E. coli/g of ground beef, respectively; f, negative control (uninoculated meat).
Article Snippet: The bacterial cultures used in this study are detailed in Table . table ft1 table-wrap mode="anchored" t5 TABLE 1
Techniques: Amplification, Negative Control
Journal: Prion
Article Title: Can plants serve as a vector for prions causing chronic wasting disease?
doi: 10.4161/pri.27963
Figure Lengend Snippet: Figure 2. Prion signal found in wheat roots exposed to CWD PrPTSE was protease sensitive but no prion signal in lower stem (Stem) extract was visible. CWD PrPTSE was purified from brain homogenate (BH) with Bio Rad-TeSeE® purification and re-suspended in phosphate buffered saline to a 1% solution (w/v) based on the initial BH solution. Wheat plant roots were exposed to the purified solution for 24 h. Normal BH processed with TeSeE® served as a negative control. Plant protein extracts were digested with proteinase K (PK) (10 µg/mL, 30 min, 37 °C) to determine PK-resistance of any proteins. Western blotting of plant protein extracts (plant total protein extraction kit) was done using P4 mAb (1:5000) and Prionics®-Check Western kit. Results are representative of three independent replicates (n = 3). Lanes 1, 3, 5, 7: plants exposed to normal BH processed with Bio Rad kit; Lanes 2, 4, 6, 8: plants exposed to CWD infected BH processed with Bio Rad kit; Lane 9: CWD infected BH (0.1%) processed with Bio Rad kit.
Article Snippet: All samples were negative when tested with IDEXX HerdChek® ( ). table ft1 table-wrap mode="anchored"
Techniques: Purification, Saline, Negative Control, Western Blot, Protein Extraction, Infection
Journal: Prion
Article Title: Can plants serve as a vector for prions causing chronic wasting disease?
doi: 10.4161/pri.27963
Figure Lengend Snippet: Table 1. Analysis of plant protein extracts (1% SDS) from proteinase K-digested (PK) CWD BH treatment with Bio-Rad and IDEXX diagnostic tests
Article Snippet: All samples were negative when tested with IDEXX HerdChek® ( ). table ft1 table-wrap mode="anchored"
Techniques: Diagnostic Assay, Control
Journal: Neuropharmacology
Article Title: Chronic stress induces cell type-selective transcriptomic and electrophysiological changes in the bed nucleus of the stria terminalis
doi: 10.1016/j.neuropharm.2019.03.013
Figure Lengend Snippet: Normalized quantities of mRNA for the Gria1 (Type I: NS n = 18, CSS n = 17; Type II: NS n = 32, CSS n = 22; Type III: NS n = 27, CSS n = 22; A) and Gria2 subunits (Type I: NS n = 18, CSS n = 17; Type II: NS n = 31, CSS n = 24; Type III: NS n = 25, CSS n = 22; B) from cells classified as Type I-III from NS (grey open squares; 20 rats) and CSS (black closed squares; 19 rats) rats. Used Mann-Whitney U-tests. Mean and SEM shown. * p < 0.002
Article Snippet: Results were analyzed using the ΔCt method ( Livak and Schmittgen 2001 , Vandesompele, De Preter et al. 2002 ). table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 Gene name (protein name) TaqMan assay reference Grial (GluRl)
Techniques: MANN-WHITNEY
Journal: Ultramicroscopy
Article Title: Application of EELS and EFTEM to the Life Sciences Enabled by the Contributions of Ondrej Krivanek
doi: 10.1016/j.ultramic.2017.01.002
Figure Lengend Snippet: STEM-EELS imaging of frozen hydrated specimens at a beam energy of 100 keV using a Gatan PEELS interfaced to a VG Microscopes HB501 STEM. (a) Low-loss spectra up to an energy loss of 30 eV from major chemical constituents of cells; these can be used to fit spectra from cryosectioned cells to give quantitative compositional information; (b) Low-dose dark-field STEM of frozen hydrated liver cryosection showing no contrast apart from deformation lines; scale bar = 1 μm; (c) Water map of hepatocytes generated by multiple least squares fitting of water and protein reference spectra at each pixel revealing: mitochondria (M), cytoplasm (C), red blood cells (R), plasma (P) and lipid droplets (L); (d) Water content histogram for 2700 pixels of cytoplasm (light bars) and 500 pixels of mitochondria (dark bars) in hepatocyte, showing approximately Gaussian peaks with half width ~5%. From S. Sun et al. [27].
Article Snippet: Other laboratories have also determined water distributions using this approach [ 28 , 29 ]. fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Figure 4
Techniques: Imaging, Generated
![Spectrum-imaging of two neuronal dendrites in the vicinity of the Ca L2,3 edge, together with dark-field images (A, E) obtained using the Gatan 666 Parallel EELS attached to a VG Microscopes HB501 STEM. Background-subtracted nitrogen K-edge maps (B, F) reveal location of mitochondria and membranes of endoplasmic reticulum. Very weak signals are detected when these nitrogen maps are segmented according to compartment: endoplasmic reticulum (C, G) and mitochondria (D, H); Bars = 200 nm. Spectra at each pixel were acquired in the difference mode with a 6 eV shift to reduce noise due to channel gain variations in the photodiode array. Multiple-least-squares fit (solid curves) of filtered reference spectra for segmented spectrum-image data (circles) in endoplasmic reticulum (J) and mitochondria (K). Analysis of the Ca L2,3 edge signal showed that the ER calcium concentration was 4.9±0.4 mmol/kg dry wt., and the mitochondrial calcium concentration was 1.4±0.4 mmol/kg dry wt. From R.D. Leapman et al. [35].](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_8483/pmc05468483/pmc05468483__nihms846160f5.jpg)
Journal: Ultramicroscopy
Article Title: Application of EELS and EFTEM to the Life Sciences Enabled by the Contributions of Ondrej Krivanek
doi: 10.1016/j.ultramic.2017.01.002
Figure Lengend Snippet: Spectrum-imaging of two neuronal dendrites in the vicinity of the Ca L2,3 edge, together with dark-field images (A, E) obtained using the Gatan 666 Parallel EELS attached to a VG Microscopes HB501 STEM. Background-subtracted nitrogen K-edge maps (B, F) reveal location of mitochondria and membranes of endoplasmic reticulum. Very weak signals are detected when these nitrogen maps are segmented according to compartment: endoplasmic reticulum (C, G) and mitochondria (D, H); Bars = 200 nm. Spectra at each pixel were acquired in the difference mode with a 6 eV shift to reduce noise due to channel gain variations in the photodiode array. Multiple-least-squares fit (solid curves) of filtered reference spectra for segmented spectrum-image data (circles) in endoplasmic reticulum (J) and mitochondria (K). Analysis of the Ca L2,3 edge signal showed that the ER calcium concentration was 4.9±0.4 mmol/kg dry wt., and the mitochondrial calcium concentration was 1.4±0.4 mmol/kg dry wt. From R.D. Leapman et al. [35].
Article Snippet: Other laboratories have also determined water distributions using this approach [ 28 , 29 ]. fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Figure 4
Techniques: Imaging, Concentration Assay
![Application of scanning transmission electron microscope-electron energy-loss spectroscopy (STEM-EELS) to explain contrast observed in brain magnetic resonance images (MRI) in terms of iron concentrations. (a) Optical micrograph of post-mortem human visual cortex treated with Perl stain for iron, showing elevated iron in the region of the line of Gennari (arrows). (b) Corresponding MRI, also showing contrast in the line of Gennari (arrows). Image widths in (a) and (b) are the same, and asterisks indicate boundaries of region of visual cortex. (c) Phase-contrast transmission electron microscopy of unstained section in the region of the line of Gennari showing electron-dense particles. (d–f) STEM-EELS iron maps obtained from randomly selected areas of an unstained specimen in the vicinity of the line of Gennari, showing particles with high Fe content. (g) Typical EELS extracted from one of the Fe-containing particles reveals a strong Fe L2,3 edge; quantitative analysis showed that the particles contained on average 1740 ± 580 Fe atoms, consistent with the iron cores of ferritin molecules; dotted lines indicate extrapolated background intensity. From M. Fukunaga et al. [36].](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_8483/pmc05468483/pmc05468483__nihms846160f6.jpg)
Journal: Ultramicroscopy
Article Title: Application of EELS and EFTEM to the Life Sciences Enabled by the Contributions of Ondrej Krivanek
doi: 10.1016/j.ultramic.2017.01.002
Figure Lengend Snippet: Application of scanning transmission electron microscope-electron energy-loss spectroscopy (STEM-EELS) to explain contrast observed in brain magnetic resonance images (MRI) in terms of iron concentrations. (a) Optical micrograph of post-mortem human visual cortex treated with Perl stain for iron, showing elevated iron in the region of the line of Gennari (arrows). (b) Corresponding MRI, also showing contrast in the line of Gennari (arrows). Image widths in (a) and (b) are the same, and asterisks indicate boundaries of region of visual cortex. (c) Phase-contrast transmission electron microscopy of unstained section in the region of the line of Gennari showing electron-dense particles. (d–f) STEM-EELS iron maps obtained from randomly selected areas of an unstained specimen in the vicinity of the line of Gennari, showing particles with high Fe content. (g) Typical EELS extracted from one of the Fe-containing particles reveals a strong Fe L2,3 edge; quantitative analysis showed that the particles contained on average 1740 ± 580 Fe atoms, consistent with the iron cores of ferritin molecules; dotted lines indicate extrapolated background intensity. From M. Fukunaga et al. [36].
Article Snippet: Other laboratories have also determined water distributions using this approach [ 28 , 29 ]. fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Figure 4
Techniques: Transmission Assay, Microscopy, Spectroscopy, Staining, Electron Microscopy
![Spatially resolved element STEM-EELS analysis of hybrid silica nanoparticles containing quantum dots and coated with lipid that bind gadolinium; HAADF image of the lipid-coated nanoparticles (a); composite color map showing the location of different elements: red, blue and green indicate gadolinium (N4,5 edge), silicon (L2,3 edge) and carbon atoms (C K edge), respectively. From M.M. van Schooneveld et al. [39].](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_8483/pmc05468483/pmc05468483__nihms846160f8.jpg)
Journal: Ultramicroscopy
Article Title: Application of EELS and EFTEM to the Life Sciences Enabled by the Contributions of Ondrej Krivanek
doi: 10.1016/j.ultramic.2017.01.002
Figure Lengend Snippet: Spatially resolved element STEM-EELS analysis of hybrid silica nanoparticles containing quantum dots and coated with lipid that bind gadolinium; HAADF image of the lipid-coated nanoparticles (a); composite color map showing the location of different elements: red, blue and green indicate gadolinium (N4,5 edge), silicon (L2,3 edge) and carbon atoms (C K edge), respectively. From M.M. van Schooneveld et al. [39].
Article Snippet: Other laboratories have also determined water distributions using this approach [ 28 , 29 ]. fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Figure 4
Techniques:
Journal: Bioorganic & medicinal chemistry
Article Title: Small Molecule Adjuvants that Suppress Both Chromosomal and mcr-1 Encoded Colistin-Resistance and Amplify Colistin Efficacy in Polymyxin-Susceptible Bacteria
doi: 10.1016/j.bmc.2017.08.055
Figure Lengend Snippet: Colstin MICs (μg/mL) in mcr- 1-carrying strains and their corresponding parent.
Article Snippet: As can be seen, the mcr-1 plasmid indeed confers increased colistin resistance in all strains at or above the CLSI breakpoint, and patients infected with these strains could not be successfully treated with colistin in its highest tolerated dosage. table ft1 table-wrap mode="anchored"
Techniques:
Journal: Bioorganic & medicinal chemistry
Article Title: Small Molecule Adjuvants that Suppress Both Chromosomal and mcr-1 Encoded Colistin-Resistance and Amplify Colistin Efficacy in Polymyxin-Susceptible Bacteria
doi: 10.1016/j.bmc.2017.08.055
Figure Lengend Snippet: Colistin MICs, given as μg/mL (fold-reduction), of mcr-1 strains treated with 1 and 2 .
Article Snippet: As can be seen, the mcr-1 plasmid indeed confers increased colistin resistance in all strains at or above the CLSI breakpoint, and patients infected with these strains could not be successfully treated with colistin in its highest tolerated dosage. table ft1 table-wrap mode="anchored"
Techniques:
Journal: Bioorganic & medicinal chemistry
Article Title: Small Molecule Adjuvants that Suppress Both Chromosomal and mcr-1 Encoded Colistin-Resistance and Amplify Colistin Efficacy in Polymyxin-Susceptible Bacteria
doi: 10.1016/j.bmc.2017.08.055
Figure Lengend Snippet: MS analysis of A. baumannii ATCC 17978+mcr-1 treated with compound 2. PEtN-modified species shown in red. All spectra were acquired in negative ion mode.
Article Snippet: As can be seen, the mcr-1 plasmid indeed confers increased colistin resistance in all strains at or above the CLSI breakpoint, and patients infected with these strains could not be successfully treated with colistin in its highest tolerated dosage. table ft1 table-wrap mode="anchored"
Techniques: Modification
Journal: Bioorganic & medicinal chemistry
Article Title: Small Molecule Adjuvants that Suppress Both Chromosomal and mcr-1 Encoded Colistin-Resistance and Amplify Colistin Efficacy in Polymyxin-Susceptible Bacteria
doi: 10.1016/j.bmc.2017.08.055
Figure Lengend Snippet: Colistin MICs, given as μg/mL (fold-reduction), of mcr-1 strains treated with compound 3 .
Article Snippet: As can be seen, the mcr-1 plasmid indeed confers increased colistin resistance in all strains at or above the CLSI breakpoint, and patients infected with these strains could not be successfully treated with colistin in its highest tolerated dosage. table ft1 table-wrap mode="anchored"
Techniques:
Journal: Bioorganic & medicinal chemistry
Article Title: Small Molecule Adjuvants that Suppress Both Chromosomal and mcr-1 Encoded Colistin-Resistance and Amplify Colistin Efficacy in Polymyxin-Susceptible Bacteria
doi: 10.1016/j.bmc.2017.08.055
Figure Lengend Snippet: Colistin MICs, given as μg/mL (fold-reduction), of colistin resistant clinical isolates treated with 3 .
Article Snippet: As can be seen, the mcr-1 plasmid indeed confers increased colistin resistance in all strains at or above the CLSI breakpoint, and patients infected with these strains could not be successfully treated with colistin in its highest tolerated dosage. table ft1 table-wrap mode="anchored"
Techniques:
Journal: Bioorganic & medicinal chemistry
Article Title: Small Molecule Adjuvants that Suppress Both Chromosomal and mcr-1 Encoded Colistin-Resistance and Amplify Colistin Efficacy in Polymyxin-Susceptible Bacteria
doi: 10.1016/j.bmc.2017.08.055
Figure Lengend Snippet: Colistin MICs, given as μg/mL (fold-reduction), of MDR isolates treated with 30 μM 1 , 2 and 3 .
Article Snippet: As can be seen, the mcr-1 plasmid indeed confers increased colistin resistance in all strains at or above the CLSI breakpoint, and patients infected with these strains could not be successfully treated with colistin in its highest tolerated dosage. table ft1 table-wrap mode="anchored"
Techniques:
Journal: Bioorganic & medicinal chemistry
Article Title: Small Molecule Adjuvants that Suppress Both Chromosomal and mcr-1 Encoded Colistin-Resistance and Amplify Colistin Efficacy in Polymyxin-Susceptible Bacteria
doi: 10.1016/j.bmc.2017.08.055
Figure Lengend Snippet: Adjuvant concentration necessary to achieve colistin MICs of 0.125 μg/mL in MDR strains.
Article Snippet: As can be seen, the mcr-1 plasmid indeed confers increased colistin resistance in all strains at or above the CLSI breakpoint, and patients infected with these strains could not be successfully treated with colistin in its highest tolerated dosage. table ft1 table-wrap mode="anchored"
Techniques: Adjuvant, Concentration Assay
Journal: Journal of Bacteriology
Article Title: Genetic, Structural, and Antigenic Analyses of Glycan Diversity in the O-Linked Protein Glycosylation Systems of Human Neisseria Species
doi: 10.1128/JB.00101-10
Figure Lengend Snippet: Neisseria strains used in this study
Article Snippet: In the high-pH treatment experiments, cells were incubated in GC media containing 50 mM NaOH at 37°C for 45 min. table ft1 table-wrap mode="anchored" t5 TABLE 1.
Techniques: Modification
Journal: Journal of Bacteriology
Article Title: Genetic, Structural, and Antigenic Analyses of Glycan Diversity in the O-Linked Protein Glycosylation Systems of Human Neisseria Species
doi: 10.1128/JB.00101-10
Figure Lengend Snippet: Neisserial glycoprotein profiles and glycan diversity. (Top panels) Immunoblotting of whole-cell lysates from strains of N. gonorrhoeae (Ngo) (N400 pglC, N400, and FA1090), N. meningitidis (Nme) (MC58, H44/76, Z2491, 8013, FAM18, BZ 10, and BZ 198), and N. lactamica (Nla) (ST-3787 and ST-640) with glycan-specific monoclonal antibodies. (Bottom panels) Immunoblotting using the SM1 MAb specifically recognizing class I pilin types and a polyclonal antiserum raised against the PilE-derived peptide 44KSAVTEYYLNHGKWPENNTSA64, which reacts with all pilin types. Thus, strains FAM18, BZ 10, ST-3787, and ST-640 all express class II pilins. Also, strains 8013, FAM18, BZ 10, and BZ 198 carry the pglB2 allele that is associated with synthesis of GATDH glycans, while all of the other strains have the pglB allele that is associated with synthesis of DATDH glycans.
Article Snippet: In the high-pH treatment experiments, cells were incubated in GC media containing 50 mM NaOH at 37°C for 45 min. table ft1 table-wrap mode="anchored" t5 TABLE 1.
Techniques: Western Blot, Derivative Assay
Journal: Journal of Bacteriology
Article Title: Genetic, Structural, and Antigenic Analyses of Glycan Diversity in the O-Linked Protein Glycosylation Systems of Human Neisseria Species
doi: 10.1128/JB.00101-10
Figure Lengend Snippet: Glycoprotein profiles and glycan antigenicity in strains N400, FA1090, and MC58. (A) Whole-cell lysates of N. gonorrhoeae N400 and FA1090 wild-type (wt) strains and pglA (monosaccharide-expressing) and pglC (glycosylation-null) mutants probed with MAb npg1. The strains used were KS100 (N400 wt), KS104 (N400 pglC), KS141 (N400 pglA), KS302 (FA1090 pglA), KS301 (FA1090 pglC), and KS300 (FA1090 wt). (B) Immunoblotting of whole-cell lysates of N. gonorrhoeae FA1090 and N. meningitidis MC58 wild-type strains and pglA (monosaccharide-expressing) and pglC (glycosylation-null) mutants probed with MAbs npg1, npg2, and npg3. The strains used were KS301 (FA1090 pglC), KS302 (FA1090 pglA), KS300 (FA1090 wt), KS317 (MC58 pglC), KS318 (MC58 pglA), and KS316 (MC58 wt).
Article Snippet: In the high-pH treatment experiments, cells were incubated in GC media containing 50 mM NaOH at 37°C for 45 min. table ft1 table-wrap mode="anchored" t5 TABLE 1.
Techniques: Expressing, Western Blot
![Increased npg2 and npg3 immunoreactivity at high pH and in pglI mutants. Immunoblotting of whole-cell lysates of wild-type strains, pglI mutants, and high-pH-treated wild-type strains revealed increased reactivity with the npg2 and npg3 monoclonal antibodies due to the lack of an O-acetyl group in pglI mutants or with the high-pH treatment. The extraneous band that appeared during high-pH treatment is PilE (as determined by immunoblotting with SM1 [data not shown]). This band was not present with the 4/3/1 background, in which pilE was conditionally repressed. The strains used were KS100 (N400 wt), KS144 (N400 pglI), KS104 (N400 pglC), KS101 (4/3/1 wt), KS300 (FA1090 wt), KS303 (FA1090 pglI), and KS301 (FA1090 pglC). wt, wild type.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_6500/pmc02876500/pmc02876500__zjb9990995680004.jpg)
Journal: Journal of Bacteriology
Article Title: Genetic, Structural, and Antigenic Analyses of Glycan Diversity in the O-Linked Protein Glycosylation Systems of Human Neisseria Species
doi: 10.1128/JB.00101-10
Figure Lengend Snippet: Increased npg2 and npg3 immunoreactivity at high pH and in pglI mutants. Immunoblotting of whole-cell lysates of wild-type strains, pglI mutants, and high-pH-treated wild-type strains revealed increased reactivity with the npg2 and npg3 monoclonal antibodies due to the lack of an O-acetyl group in pglI mutants or with the high-pH treatment. The extraneous band that appeared during high-pH treatment is PilE (as determined by immunoblotting with SM1 [data not shown]). This band was not present with the 4/3/1 background, in which pilE was conditionally repressed. The strains used were KS100 (N400 wt), KS144 (N400 pglI), KS104 (N400 pglC), KS101 (4/3/1 wt), KS300 (FA1090 wt), KS303 (FA1090 pglI), and KS301 (FA1090 pglC). wt, wild type.
Article Snippet: In the high-pH treatment experiments, cells were incubated in GC media containing 50 mM NaOH at 37°C for 45 min. table ft1 table-wrap mode="anchored" t5 TABLE 1.
Techniques: Western Blot
Journal: Journal of Bacteriology
Article Title: Genetic, Structural, and Antigenic Analyses of Glycan Diversity in the O-Linked Protein Glycosylation Systems of Human Neisseria Species
doi: 10.1128/JB.00101-10
Figure Lengend Snippet: Protein glycosylation genotypes and phenotypes
Article Snippet: In the high-pH treatment experiments, cells were incubated in GC media containing 50 mM NaOH at 37°C for 45 min. table ft1 table-wrap mode="anchored" t5 TABLE 1.
Techniques: