Journal: Physiological Research

Article Title: The Severity of Muscle Performance Deterioration in Sarcopenia Correlates With Circulating Muscle Tissue-Specific miRNAs

doi: 10.33549/physiolres.934778

Figure Lengend Snippet: List of microRNA primers (Qiagen GmbH, Germany)

Article Snippet: The PCR reaction was performed in a StepOnePlus Real-Time PCR System (Life Technologies, USA). lists the PCR primers used in this study. table ft1 table-wrap mode="anchored" t5 caption a7 microRNA LNA PCR primer ID microRNA LNA PCR primer ID cel-miR-39-3p YP00203952 hsa-miR-133a-3p YP00204788 hsa-miR-1-3p YP00204344 hsa-miR-133b YP00206058 hsa-miR-206 YP00206073 hsa-miR-208b-3p YP00204636 hsa-miR-29a-3p YP00204698 hsa-miR-499-5p YP00205935 hsa-miR-29b-3p YP00204679 Open in a separate window List of microRNA primers (Qiagen GmbH, Germany) Statistical analysis Statistical evaluation was performed by Graphpad using one way ANOVA test and Chi test.

Techniques:

Journal: Transplantation

Article Title: Single Cell Transcriptomics and Solid Organ Transplantation

doi: 10.1097/TP.0000000000002725

Figure Lengend Snippet: A comparison of current high throughput droplet based single cell RNA-seq methods

Article Snippet: Fortunately, an increasing variety of programs are simplifying this process which lowers the bar of entry for laboratories without coding experience. table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 DropSeq InDrops 10X Chromium Year developed 2015 2015 2016 Commercial platform No 1CellBio 10X Genomics Full length sequence No No No 3’ or 5’ sequence 3’ 3’ both Capture efficiency 12.8% 90% 65% Sensitivity (molecule detection limit) 1e+1 1e+0.5 1e+2.5 Accuracy (Pearson R) >0.9 >0.9 >0.9 Cell capacity per run 10,000 10,000 80,000 Open in a separate window A comparison of current high throughput droplet based single cell RNA-seq methods Although beyond the scope of this review, a variety of machine learning techniques allow for analysis and data visualization ( ).

Techniques: Comparison, High Throughput Screening Assay, Sequencing