Journal: Bioscience Reports

Article Title: Inhibition of calpain-1 stabilizes TCF11/Nrf1 but does not affect its activation in response to proteasome inhibition

doi: 10.1042/BSR20180393

Figure Lengend Snippet: ( A and B ) Ea.Hy-926 cells were transfected with siRNA against CAPNS1 or an OFF-Target siRNA. (A) Twenty-four hours later cells were treated with 30 nM BTZ for 16 h, total cell lysates were analysed by immunoblot, representative image from n =3, (B) RNA was isolated and used for qRT-PCR to analyse expression of PSMA3, PSMB6 and PSMC4 , values were calcuated using the ΔΔ C T method with HPRT-1 as housekeeping gene and the normalised to samples without BTZ treatment (set to 1), mean ± SEM, n =3. ( C ) Ea.Hy-926 cells were transfected with flag-tagged constructs for the large subunits of calpain-1 (CAPN1) and -2 (CAPN2). The following day, the cells were treated with 30 nM BTZ for 16 h, total cell lysates were analysed by immunoblot, representative image from n =3.

Article Snippet: The flag-tagged CAPN1 (Addgene #60941) and CAPN2 (Addgene #60942) have been previously described [ ].

Techniques: Transfection, Western Blot, Isolation, Quantitative RT-PCR, Expressing, Construct

Journal: Oncotarget

Article Title: Could a plant derived protein potentiate the anticancer effects of a stem cell in brain cancer?

doi: 10.18632/oncotarget.25090

Figure Lengend Snippet: The EcTI effects on the protease expression in U87 cells were measured by qRT-PCR analysis and compared to non-treated control (c) cells.The effect of EcTI on the expression of the metalloproteases Mmp2 (A) , Mmp9 (B) , Mmp14 (C) , urokinase plasminogen activator, Plau (D) and its receptor, PlauR (E) , Calpain 1, Capn1 (F) , Calpain 2, Capn2 (G) , Cathepsin L, CtsL (H) , and Cathepsin B, CtsB (I) . Error bars indicate SD. * p < 0.05, ** p< 0.005, *** p< 0.0005 were considered significant.

Article Snippet: The real-time PCR reactions were performed using 1:10 dilutions (1 μl/well) of each cDNA sample, TaqMan Universal PCR Master Mix (Applied Biosystems, USA), and TaqMan Gene Expression assays (all from Applied Biosystems, USA) for: Ccnd1 (Cyclin D1), Hs00765553_m1; Cdkn1A (cyclin-dependent kinase inhibitor 1A (p21, Cip1)), Hs00355782_m1; Tp53 (tumor protein p53), Hs01034249_m1; Bax (Bcl-2 associated X protein), Hs99999001_m1; Bcl2 (B-cell CLL/lymphoma2), Hs00608023_m1; Mmp14 (matrix metallopeptidase 14 (membrane-inserted)), Hs00237119_m1; Mmp2 (matrix metallopeptidase 2), Hs1548727_m1; Mmp9 (matrix metallopeptidase 9), Hs00234579_m1; Plaur (plasminogen activator, urokinase receptor), Hs00958880_m1; Plau (plasminogen activator, urokinase), Hs01547054_m1; Capn1 (calpain 1, (mu/l) large subunit), Hs00559804_m1; Capn2 (calpain 2, (m/II) large subunit), Hs00965097_m1; CtsB (cathepsin B), Hs00947433; and CtsL (cathepsin L), Hs00964650_m1.

Techniques: Expressing, Quantitative RT-PCR, Control

Journal:

Article Title: Statin Therapy and the Expression of Genes that Regulate Calcium Homeostasis and Membrane Repair in Skeletal Muscle

doi: 10.2353/ajpath.2010.091140

Figure Lengend Snippet: Genes Selected for Molecular Analysis

Article Snippet: Calpain2 , CAPN2 Hs00156251_m1 , 0.372 , 0.247.

Techniques: Binding Assay