Journal: PLoS ONE

Article Title: Antibacterial Responses by Peritoneal Macrophages Are Enhanced Following Vitamin D Supplementation

doi: 10.1371/journal.pone.0116530

Figure Lengend Snippet: Clinical and biological data for PD patients at baseline.

Article Snippet: Probe and primer sets were as follows: Hs00172113-m1 ( VDR) , Hs00168017-m1 ( CYP27B1) , Hs00167999-m1 ( CYP24A1) , Hs00189038-m1 ( CAMP) and Hs00221783_m1 ( HAMP) (Applied Biosystems).

Techniques: Clinical Proteomics

Journal: PLoS ONE

Article Title: Antibacterial Responses by Peritoneal Macrophages Are Enhanced Following Vitamin D Supplementation

doi: 10.1371/journal.pone.0116530

Figure Lengend Snippet: Correlation between PD cell mRNA expression of CAMP or HAMP and: A) donor patient serum concentrations of 25D (ng/ml); B) CYP27B1 mRNA; C) VDR mRNA in PD cells isolated from CKD patient dialysate effluents from n = 27 baseline samples (pre-vitamin D supplementation) from 27 different patients. Data for mRNA expression are shown as 1/ΔCt values.

Article Snippet: Probe and primer sets were as follows: Hs00172113-m1 ( VDR) , Hs00168017-m1 ( CYP27B1) , Hs00167999-m1 ( CYP24A1) , Hs00189038-m1 ( CAMP) and Hs00221783_m1 ( HAMP) (Applied Biosystems).

Techniques: Expressing, Isolation

Journal: PLoS ONE

Article Title: Antibacterial Responses by Peritoneal Macrophages Are Enhanced Following Vitamin D Supplementation

doi: 10.1371/journal.pone.0116530

Figure Lengend Snippet: Effect of 1,25D (5 nM, 6 hrs, n = 13) (A) or 25D (100 nM, 6 hrs, n = 8) (B) on expression for mRNA for CYP27B1 , VDR , CAMP and HAMP in PD cells cultured in RPMI with 10% human serum and GM-CSF (10 IU/mL). Data are shown as fold-change in mRNA expression relative to vehicle (0.1% ethanol)-treated cells for each gene with vehicle expression = 1 (see dashed line). * = statistically different from vehicle-treated cells, p<0.05. *** = statistically different from vehicle-treated cells, p<0.001.

Article Snippet: Probe and primer sets were as follows: Hs00172113-m1 ( VDR) , Hs00168017-m1 ( CYP27B1) , Hs00167999-m1 ( CYP24A1) , Hs00189038-m1 ( CAMP) and Hs00221783_m1 ( HAMP) (Applied Biosystems).

Techniques: Expressing, Cell Culture

Journal: PLoS ONE

Article Title: Antibacterial Responses by Peritoneal Macrophages Are Enhanced Following Vitamin D Supplementation

doi: 10.1371/journal.pone.0116530

Figure Lengend Snippet: (A) Relative expression of mRNA for CYP27B1 , VDR , CYP24A1 , CAMP and HAMP in PD cells from peritonitis patients (n = 3) compared to PD cells from non-infected patients (n = 8). Data are shown as fold-change in mRNA expression relative to non-infected cells. (B) Effect of 1,25D (5 nM, 6hrs) or 25D (100 nM, 6 hrs) on expression for mRNA for CAMP (grey bars) or HAMP (black bars) in PD cells from peritonitis patients (n = 3) RPMI with 10% human serum and GM-CSF (10 IU/mL). Data are shown as fold-change in mRNA expression relative to non-infected PD cells (3A) or vehicle (0.1% ethanol)-treated cells (3B). * = statistically different from vehicle-treated (0.1% ethanol) cells, p<0.05. ** = statistically different from vehicle-treated cells, p<0.01.

Article Snippet: Probe and primer sets were as follows: Hs00172113-m1 ( VDR) , Hs00168017-m1 ( CYP27B1) , Hs00167999-m1 ( CYP24A1) , Hs00189038-m1 ( CAMP) and Hs00221783_m1 ( HAMP) (Applied Biosystems).

Techniques: Expressing, Infection