calretinin Search Results


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  • 92
    Thermo Fisher calretinin
    Immunohistochemical staining of the neoplasia. Diffuse cytoplasmic staining with cytokeratin 7 (A) and cytokeratin 5,6 (B). <t>Calretinin</t> (C) stains intensely both the nuclei and the cytoplasm of the neoplastic cells. Epithelial membrane antigen (EMA) (D)
    Calretinin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 265 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Millipore calretinin
    Characterization of cultured myenteric neurons. A , B , <t>Calretinin-immunoreactive</t> neurons are numerous in cultured myenteric ganglia. Neurons are characterized by short dendrites ( B ). C , Calbindin-immunoreactive neurons are also found in cultured myenteric ganglia; however, they have a relatively smooth appearance. Scale bars, 30 μm.
    Calretinin, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 993 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Abcam calretinin
    A: Sheets of tumor cells forming Call-Exner bodies (40x). B: Sinus histiocytosis observed in enlarged lymph nodes both along the abdominal aorta as well as in the peripheral subcutaneous tissue (40x). C: The histologic diagnosis of granulosa cell tumor was confirmed <t>calretinin</t> staining (1:100; 60x). D: Immunoreactivity of tumor infiltrating lymphocytes but not tumor cells for CD45 (1:20; 40x).
    Calretinin, supplied by Abcam, used in various techniques. Bioz Stars score: 92/100, based on 175 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Oncogene Science Inc calretinin
    Ototoxic injury and regeneration in organotypic cultures of the chick utricle. Hair cells are immunolabeled for <t>calretinin</t> ( red ) and stereocilia bundles, and cell–cell junctions are labeled with phalloidin ( green ). A Numerous hair cells ( red ) were present in utricles that were cultured for 8 days in normal medium. B Treatment for 24 h with 1 mM streptomycin caused a dramatic reduction in the numbers of labeled hair cells ( red ) and stereocilia bundles. C After 7 days recovery from streptomycin injury, numerous regenerated hair cells ( red ) were evident. Scale bar = 20 µm.
    Calretinin, supplied by Oncogene Science Inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Roche calretinin
    Micrograph immunohistochemical positive reaction for <t>calretinin</t> (original magnification ×400).
    Calretinin, supplied by Roche, used in various techniques. Bioz Stars score: 92/100, based on 41 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Swant calretinin
    CaMKIIα-hKO mice, a representative mouse model of immature dentate gyrus' (iDG), possess a perpetually immature hippocampal formation. Expression of early neuronal markers is elevated in mutant mice <t>(calretinin</t> shownin a , b ), which display a deficit in the mature neuronal marker calbindin ( c , d ). Neurogenesis is substantially increased in the dentate gyrus of mutant iDG mice ( e ). Scale bar=100 μm. *** P
    Calretinin, supplied by Swant, used in various techniques. Bioz Stars score: 92/100, based on 949 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Agilent technologies calretinin
    Immunohistochemical staining of the tumor. (A) The cells were entirely positive for CK. (B) The tumor cells were strong positive for <t>Calretinin.</t> (C) Negative expression of CEA was present in the tumor cells. (D) Ki67 index was approximately 50% in mesothelima
    Calretinin, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 93/100, based on 336 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Santa Cruz Biotechnology calretinin
    Immunohistochemistry of AII amacrine cells, staining (green) for <t>calretinin-positive</t> ( arrows ) (A) , and quantitative measurement of cell numbers (B) . AII amacrine cells were lost after 7 days of OH and protected by 50 mg/kg SNJ-1945. Scale bar=20 μm.
    Calretinin, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 84 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Ventana Medical calretinin
    Histopathological sections showing tumor cells with glandular and papillary, cord-like structures (A) and a hobnail-like appearance of tumor cells with irregular and hyperchromatic nuclei resembling malignant mesothelioma (B). Immunohistochemical evaluation demonstrates the tumor cells to be positive for thyroid transcription factor-1 (C) and negative for <t>calretinin</t> (D).
    Calretinin, supplied by Ventana Medical, used in various techniques. Bioz Stars score: 92/100, based on 39 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Nichirei calretinin
    Histopathological sections showing tumor cells with glandular and papillary, cord-like structures (A) and a hobnail-like appearance of tumor cells with irregular and hyperchromatic nuclei resembling malignant mesothelioma (B). Immunohistochemical evaluation demonstrates the tumor cells to be positive for thyroid transcription factor-1 (C) and negative for <t>calretinin</t> (D).
    Calretinin, supplied by Nichirei, used in various techniques. Bioz Stars score: 92/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96
    Abcam rabbit polyclonal anti calretinin
    Histopathological sections showing tumor cells with glandular and papillary, cord-like structures (A) and a hobnail-like appearance of tumor cells with irregular and hyperchromatic nuclei resembling malignant mesothelioma (B). Immunohistochemical evaluation demonstrates the tumor cells to be positive for thyroid transcription factor-1 (C) and negative for <t>calretinin</t> (D).
    Rabbit Polyclonal Anti Calretinin, supplied by Abcam, used in various techniques. Bioz Stars score: 96/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Agilent technologies antihuman calretinin
    Histopathological sections showing tumor cells with glandular and papillary, cord-like structures (A) and a hobnail-like appearance of tumor cells with irregular and hyperchromatic nuclei resembling malignant mesothelioma (B). Immunohistochemical evaluation demonstrates the tumor cells to be positive for thyroid transcription factor-1 (C) and negative for <t>calretinin</t> (D).
    Antihuman Calretinin, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 85/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Millipore mouse calretinin
    Histopathological sections showing tumor cells with glandular and papillary, cord-like structures (A) and a hobnail-like appearance of tumor cells with irregular and hyperchromatic nuclei resembling malignant mesothelioma (B). Immunohistochemical evaluation demonstrates the tumor cells to be positive for thyroid transcription factor-1 (C) and negative for <t>calretinin</t> (D).
    Mouse Calretinin, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Millipore anti calretinin antibody
    ON cone bipolar cell subtypes were identified by immunohistochemistry after physiological recordings. The IPLs were marked using immunolabeling with <t>calretinin</t> or ChAT antibody. A , Subtype 5s ON bipolar cells (neurobiotin, green) identified with ChAT
    Anti Calretinin Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 80 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Millipore mouse anti calretinin
    Morphologic characteristics of Prdm16+ ganglion cells. (A–D) A Thy1-YFP-H transgenic mouse retinal flatmount stained with antibodies to GFP (green); Prdm16 (red); and <t>calretinin</t> (gray). One round Prdm16+ nucleus coexpresses YFP (arrows) and its
    Mouse Anti Calretinin, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 416 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Novus Biologicals rabbit calretinin
    A subset of mCHERRY-positive cell bodies in the midbrain, the LHb, the PMH, and the RMN are immuno-positive for CALBINDIN (CALB1) and <t>CALRETININ</t> (CALB2). Immunofluorescent histological analysis of ( A–G ) mCHERRY (red) and CALB1 (green) and ( H–N ) mCHERRY (red) and CALB2 (green) in the ( A , H ) midbrain, ( B , I ) arcuate nucleus, and ( C , J ) premammillary of the hypothalamus (PMH) ( D , K ), the lateral septum, ( E , L ) LHb, ( F , M ) BLP of the amygdala, and ( G , N ) RMN (yellow arrows illustrate example of cells where co-localization is detected; scale bar: 50 μm). Arc, arcuate nucleus; D3V, dorsal third ventricle; LS, lateral septum; MHb, medial habenula; PV, paraventricular thalamic nucleus; 3V, third ventricle; Arc, Arcuate nucleus; BLP, Basolateral amygdaloid nucleus posterior division; D3V, Dorsal third ventricle; LHb, Lateral habenula; LS, Lateral septum; MHb, Medial habenula; PMH, Premammillary nucleus of the hypothalamus; PV, Paraventricular thalamic nucleus; RMN, Retromammillary; SNc, Substantia nigra pars compacta; VTA: Ventral tegmental area; 3V, Third ventricle.
    Rabbit Calretinin, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Novus Biologicals calretinin antibody
    A subset of mCHERRY-positive cell bodies in the midbrain, the LHb, the PMH, and the RMN are immuno-positive for CALBINDIN (CALB1) and <t>CALRETININ</t> (CALB2). Immunofluorescent histological analysis of ( A–G ) mCHERRY (red) and CALB1 (green) and ( H–N ) mCHERRY (red) and CALB2 (green) in the ( A , H ) midbrain, ( B , I ) arcuate nucleus, and ( C , J ) premammillary of the hypothalamus (PMH) ( D , K ), the lateral septum, ( E , L ) LHb, ( F , M ) BLP of the amygdala, and ( G , N ) RMN (yellow arrows illustrate example of cells where co-localization is detected; scale bar: 50 μm). Arc, arcuate nucleus; D3V, dorsal third ventricle; LS, lateral septum; MHb, medial habenula; PV, paraventricular thalamic nucleus; 3V, third ventricle; Arc, Arcuate nucleus; BLP, Basolateral amygdaloid nucleus posterior division; D3V, Dorsal third ventricle; LHb, Lateral habenula; LS, Lateral septum; MHb, Medial habenula; PMH, Premammillary nucleus of the hypothalamus; PV, Paraventricular thalamic nucleus; RMN, Retromammillary; SNc, Substantia nigra pars compacta; VTA: Ventral tegmental area; 3V, Third ventricle.
    Calretinin Antibody, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 92/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Thermo Fisher recombinant calretinin
    A subset of mCHERRY-positive cell bodies in the midbrain, the LHb, the PMH, and the RMN are immuno-positive for CALBINDIN (CALB1) and <t>CALRETININ</t> (CALB2). Immunofluorescent histological analysis of ( A–G ) mCHERRY (red) and CALB1 (green) and ( H–N ) mCHERRY (red) and CALB2 (green) in the ( A , H ) midbrain, ( B , I ) arcuate nucleus, and ( C , J ) premammillary of the hypothalamus (PMH) ( D , K ), the lateral septum, ( E , L ) LHb, ( F , M ) BLP of the amygdala, and ( G , N ) RMN (yellow arrows illustrate example of cells where co-localization is detected; scale bar: 50 μm). Arc, arcuate nucleus; D3V, dorsal third ventricle; LS, lateral septum; MHb, medial habenula; PV, paraventricular thalamic nucleus; 3V, third ventricle; Arc, Arcuate nucleus; BLP, Basolateral amygdaloid nucleus posterior division; D3V, Dorsal third ventricle; LHb, Lateral habenula; LS, Lateral septum; MHb, Medial habenula; PMH, Premammillary nucleus of the hypothalamus; PV, Paraventricular thalamic nucleus; RMN, Retromammillary; SNc, Substantia nigra pars compacta; VTA: Ventral tegmental area; 3V, Third ventricle.
    Recombinant Calretinin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Immunohistochemical staining of the neoplasia. Diffuse cytoplasmic staining with cytokeratin 7 (A) and cytokeratin 5,6 (B). Calretinin (C) stains intensely both the nuclei and the cytoplasm of the neoplastic cells. Epithelial membrane antigen (EMA) (D)

    Journal:

    Article Title: Pleural effusion: what lies underneath?

    doi: 10.1136/bcr.09.2008.0973

    Figure Lengend Snippet: Immunohistochemical staining of the neoplasia. Diffuse cytoplasmic staining with cytokeratin 7 (A) and cytokeratin 5,6 (B). Calretinin (C) stains intensely both the nuclei and the cytoplasm of the neoplastic cells. Epithelial membrane antigen (EMA) (D)

    Article Snippet: We used a combination of six immunohistochemical markers: cytokeratin (CK)7 (Zymed Laboratories, San Francisco, California, USA), cytokeratin 20 (Labvision, Fremont, California, USA), cytokeratin 5/6 (Zymed), calretinin (Zymed), thyroid transcription factor (TTF1; Novocastra, Leica Microsystems GmbH, Wetzlar, Germany) and epithelial membrane antigen (EMA; Dako Corporation, Glostrup, Denmark).

    Techniques: Immunohistochemistry, Staining

    Characterization of cultured myenteric neurons. A , B , Calretinin-immunoreactive neurons are numerous in cultured myenteric ganglia. Neurons are characterized by short dendrites ( B ). C , Calbindin-immunoreactive neurons are also found in cultured myenteric ganglia; however, they have a relatively smooth appearance. Scale bars, 30 μm.

    Journal: The Journal of Neuroscience

    Article Title: Excitotoxicity in the Enteric Nervous System

    doi: 10.1523/JNEUROSCI.17-22-08804.1997

    Figure Lengend Snippet: Characterization of cultured myenteric neurons. A , B , Calretinin-immunoreactive neurons are numerous in cultured myenteric ganglia. Neurons are characterized by short dendrites ( B ). C , Calbindin-immunoreactive neurons are also found in cultured myenteric ganglia; however, they have a relatively smooth appearance. Scale bars, 30 μm.

    Article Snippet: Immunoreactivity was then demonstrated by incubating the tissues with affinity-purified rabbit polyclonal antibodies (24–48 hr; 4°C) to GluR1 and GluR2/3 (0.5 μg/ml; ), EAAC1 (0.06 μg/ml; ), GluR5/6/7 , or NSE (1:3000; Polysciences, Warrington, PA); mouse monoclonal antibodies to calbindin (CBP; 1:100; Sigma) or nonphosphorylated neurofilament H (SMI-32; 1:500; Sternberger Monoclonals); or goat antibodies to calretinin (1:6000; Chemicon, Temecula, CA) (see Table ).

    Techniques: Cell Culture

    CRH-expressing Cajal-Retzius-like neurons demonstrate unique neurochemical characteristics. A , CRH-immunoreactive neurons in the outer molecular layer of the dentate gyrus of the P5 rat ( arrow ) are aligned along the hippocampal fissure ( asterisks ) and possess a prominent thick dendrite with branches descending to the pial surface, typical of Cajal-Retzius cells. B , These Cajal-Retzius cell-like CRH-IR neurons ( straight arrow ) do not coexpress GAD67 mRNA ( curved arrows ). C , D , CRH-IR Cajal-Retzius-like neurons ( arrows ) do not coexpress neurochemical markers ascribed to subpopulations of rat Cajal-Retzius cells: calretinin ( C ), shown as granular black deposits ( curved arrows ) or reelin mRNA ( D , curved arrows ). E , F , In contrast, neurons colocalizing calretinin and reelin mRNA ( arrowheads ) are frequently observed near the hippocampal fissure. G , H , CRH-expressing neurons in the inner molecular layer of the dentate gyrus are morphologically distinct from the Cajal-Retzius-like population abutting the hippocampal fissure but demonstrate similar neurochemical characteristics and do not coexpress GAD67 mRNA ( blue , curved arrows ). Scale bar, 25 μ m.

    Journal: The Journal of neuroscience : the official journal of the Society for Neuroscience

    Article Title: Novel and Transient Populations of Corticotropin-Releasing Hormone-Expressing Neurons in Developing Hippocampus Suggest Unique Functional Roles: A Quantitative Spatiotemporal Analysis

    doi:

    Figure Lengend Snippet: CRH-expressing Cajal-Retzius-like neurons demonstrate unique neurochemical characteristics. A , CRH-immunoreactive neurons in the outer molecular layer of the dentate gyrus of the P5 rat ( arrow ) are aligned along the hippocampal fissure ( asterisks ) and possess a prominent thick dendrite with branches descending to the pial surface, typical of Cajal-Retzius cells. B , These Cajal-Retzius cell-like CRH-IR neurons ( straight arrow ) do not coexpress GAD67 mRNA ( curved arrows ). C , D , CRH-IR Cajal-Retzius-like neurons ( arrows ) do not coexpress neurochemical markers ascribed to subpopulations of rat Cajal-Retzius cells: calretinin ( C ), shown as granular black deposits ( curved arrows ) or reelin mRNA ( D , curved arrows ). E , F , In contrast, neurons colocalizing calretinin and reelin mRNA ( arrowheads ) are frequently observed near the hippocampal fissure. G , H , CRH-expressing neurons in the inner molecular layer of the dentate gyrus are morphologically distinct from the Cajal-Retzius-like population abutting the hippocampal fissure but demonstrate similar neurochemical characteristics and do not coexpress GAD67 mRNA ( blue , curved arrows ). Scale bar, 25 μ m.

    Article Snippet: After CRH detection, sections were rinsed, incubated in 5% normal horse serum for 30 min, and then exposed to calretinin antiserum (1:20,000; Chemicon, Temecula, CA) for 36 –48 hr at 4°C.

    Techniques: Expressing

    Histological analysis of the PFCs of TgDyrk1A mice. A , Density of the different inhibitory neuronal populations represented by the parvalbumin (PV), calretinin (CR), and somatostatin (SOM) positive cells in the PFC. B Ratio of the vesicular VGLUT1 (excitatory) versus VGAT (inhibitory) presynaptic markers as an index of the excitatory–inhibitory balance. C , Number of presynaptic inhibitory and excitatory punctae in the PFC. D , Confocal images showing VGLUT1 (green) and VGAT (red) punctae in WT (top) and TgDyrk1A (bottom) PFC. E , F , Number of postsynaptic inhibitory (Gephyrin; E ) and excitatory (PSD-95; F ) sites. G , Density of excitatory and inhibitory terminals contacting with excitatory pyramidal neurons. H , Single confocal images illustrating inhibitory terminals (red) contacting the soma of pyramidal neurons (green) in WT (top) and TgDyrk1A (bottom) PFC. I , Density of excitatory and inhibitory terminals contacting with parvalbumin positive neurons. J , Representative confocal images of inhibitory perisomatic contacts (red) on parvalbumin positive neurons (green) of WT (top) and TgDyrk1A (bottom) PFC. Blue bars represent WT mice, and red bars represent TgDyrk1A mice. Scale bars: D , 5 μm; H , J , 1 μm. Arrows show colocalization of VGAT puncta with neuronal soma. Data are represented as mean ± SE. * p

    Journal: The Journal of Neuroscience

    Article Title: Overexpression of Dyrk1A, a Down Syndrome Candidate, Decreases Excitability and Impairs Gamma Oscillations in the Prefrontal Cortex

    doi: 10.1523/JNEUROSCI.2517-15.2016

    Figure Lengend Snippet: Histological analysis of the PFCs of TgDyrk1A mice. A , Density of the different inhibitory neuronal populations represented by the parvalbumin (PV), calretinin (CR), and somatostatin (SOM) positive cells in the PFC. B Ratio of the vesicular VGLUT1 (excitatory) versus VGAT (inhibitory) presynaptic markers as an index of the excitatory–inhibitory balance. C , Number of presynaptic inhibitory and excitatory punctae in the PFC. D , Confocal images showing VGLUT1 (green) and VGAT (red) punctae in WT (top) and TgDyrk1A (bottom) PFC. E , F , Number of postsynaptic inhibitory (Gephyrin; E ) and excitatory (PSD-95; F ) sites. G , Density of excitatory and inhibitory terminals contacting with excitatory pyramidal neurons. H , Single confocal images illustrating inhibitory terminals (red) contacting the soma of pyramidal neurons (green) in WT (top) and TgDyrk1A (bottom) PFC. I , Density of excitatory and inhibitory terminals contacting with parvalbumin positive neurons. J , Representative confocal images of inhibitory perisomatic contacts (red) on parvalbumin positive neurons (green) of WT (top) and TgDyrk1A (bottom) PFC. Blue bars represent WT mice, and red bars represent TgDyrk1A mice. Scale bars: D , 5 μm; H , J , 1 μm. Arrows show colocalization of VGAT puncta with neuronal soma. Data are represented as mean ± SE. * p

    Article Snippet: To characterize subpopulations of interneurons, we used antiparvalbumin (1:1500; Sigma), anti-calretinin (1:1000; Sigma), and anti-somatostatin (1:500; Millipore) antibodies.

    Techniques: Mouse Assay

    A: Sheets of tumor cells forming Call-Exner bodies (40x). B: Sinus histiocytosis observed in enlarged lymph nodes both along the abdominal aorta as well as in the peripheral subcutaneous tissue (40x). C: The histologic diagnosis of granulosa cell tumor was confirmed calretinin staining (1:100; 60x). D: Immunoreactivity of tumor infiltrating lymphocytes but not tumor cells for CD45 (1:20; 40x).

    Journal: Bone

    Article Title: Low magnitude mechanical signals mitigate osteopenia without compromising longevity in an aged murine model of spontaneous granulosa cell ovarian cancer

    doi: 10.1016/j.bone.2012.05.004

    Figure Lengend Snippet: A: Sheets of tumor cells forming Call-Exner bodies (40x). B: Sinus histiocytosis observed in enlarged lymph nodes both along the abdominal aorta as well as in the peripheral subcutaneous tissue (40x). C: The histologic diagnosis of granulosa cell tumor was confirmed calretinin staining (1:100; 60x). D: Immunoreactivity of tumor infiltrating lymphocytes but not tumor cells for CD45 (1:20; 40x).

    Article Snippet: Diagnosis of granulosa cell tumors by histologic examination was confirmed by immunohistochemical staining with calretinin ( Abcam; Cambridge, MA ), a molecular marker that is known to be widely expressed in GCT's.

    Techniques: Staining

    Ototoxic injury and regeneration in organotypic cultures of the chick utricle. Hair cells are immunolabeled for calretinin ( red ) and stereocilia bundles, and cell–cell junctions are labeled with phalloidin ( green ). A Numerous hair cells ( red ) were present in utricles that were cultured for 8 days in normal medium. B Treatment for 24 h with 1 mM streptomycin caused a dramatic reduction in the numbers of labeled hair cells ( red ) and stereocilia bundles. C After 7 days recovery from streptomycin injury, numerous regenerated hair cells ( red ) were evident. Scale bar = 20 µm.

    Journal: JARO: Journal of the Association for Research in Otolaryngology

    Article Title: Maintained Expression of the Planar Cell Polarity Molecule Vangl2 and Reformation of Hair Cell Orientation in the Regenerating Inner Ear

    doi: 10.1007/s10162-010-0209-4

    Figure Lengend Snippet: Ototoxic injury and regeneration in organotypic cultures of the chick utricle. Hair cells are immunolabeled for calretinin ( red ) and stereocilia bundles, and cell–cell junctions are labeled with phalloidin ( green ). A Numerous hair cells ( red ) were present in utricles that were cultured for 8 days in normal medium. B Treatment for 24 h with 1 mM streptomycin caused a dramatic reduction in the numbers of labeled hair cells ( red ) and stereocilia bundles. C After 7 days recovery from streptomycin injury, numerous regenerated hair cells ( red ) were evident. Scale bar = 20 µm.

    Article Snippet: Vestibular hair cells were labeled with a rabbit polyclonal antibody raised against calretinin (Ab-1, Oncogene Sciences; diluted 1:500).

    Techniques: Immunolabeling, Labeling, Cell Culture

    Micrograph immunohistochemical positive reaction for calretinin (original magnification ×400).

    Journal: The American Journal of Case Reports

    Article Title: Positive TTF-1 Expression in Malignant Mesothelioma: A Case Report

    doi: 10.12659/AJCR.895661

    Figure Lengend Snippet: Micrograph immunohistochemical positive reaction for calretinin (original magnification ×400).

    Article Snippet: They were also immunohistochemically stained with primary antibodies cytokeratin 5/6 (Cell Marque, Cell Marque Corporation, Rocklin, California, USA), cytokeratin 7 (Roche Diagnostics International AG, Rotkreuz, Switzerland), cytokeratin 20 (Roche Diagnostics International AG, Rotkreuz, Switzerland), calretinin (Roche Diagnostics International AG, Rotkreuz, Switzerland), and TTF-1 (Roche Diagnostics International AG, Rotkreuz, Switzerland) using the ultra-view™ universal alkaline phosphatase red detection kit (Roche Diagnostics International AG, Rotkreuz, Switzerland) on a Benchmark XT device (Ventana Medical Systems, Inc).

    Techniques: Immunohistochemistry

    CaMKIIα-hKO mice, a representative mouse model of immature dentate gyrus' (iDG), possess a perpetually immature hippocampal formation. Expression of early neuronal markers is elevated in mutant mice (calretinin shownin a , b ), which display a deficit in the mature neuronal marker calbindin ( c , d ). Neurogenesis is substantially increased in the dentate gyrus of mutant iDG mice ( e ). Scale bar=100 μm. *** P

    Journal: Translational Psychiatry

    Article Title: Detection of an immature dentate gyrus feature in human schizophrenia/bipolar patients

    doi: 10.1038/tp.2012.56

    Figure Lengend Snippet: CaMKIIα-hKO mice, a representative mouse model of immature dentate gyrus' (iDG), possess a perpetually immature hippocampal formation. Expression of early neuronal markers is elevated in mutant mice (calretinin shownin a , b ), which display a deficit in the mature neuronal marker calbindin ( c , d ). Neurogenesis is substantially increased in the dentate gyrus of mutant iDG mice ( e ). Scale bar=100 μm. *** P

    Article Snippet: Primary antibodies included: BrdU (rat monoclonal, 1:500, Abcam; Cambridge, MA, USA), Calbindin (rabbit polyclonal, 1:1000; Swant, Bellinoza, Switzerland) and Calretinin (rabbit polyclonal, 1:1000; Swant).

    Techniques: Mouse Assay, Expressing, Mutagenesis, Marker

    Identification of immature dentate gyrus' (iDG) in a subset of schizophrenia/bipolar disorder (SCZ/BPD) patients. Expression of ( a ) calretinin, ( b ) calbindin and ( c ) PCNA ratios within the dentate gyrus (DG) of SCZ, BPD and major depressive disorder (MDD) cohorts. Calretinin was consistently overexpressed in DG neurons in SZ/BPD patients vscontrol ( d2–f2 ; boxed areas magnified in d3–f3 ; DAPI d1–f1 ). Elevated calretinin expression is positively associated with ( g ) suicide death, ( h ) psychosis diagnosis and ( i ) disease duration and ( j )negatively associated with age at disease onset. ( i–j ) SCZ = green, BPD = blue, MDD = brown. Scale bars: 1 mm ( d–f1, 2 ); 100 μm ( d–f3 ). P values: *

    Journal: Translational Psychiatry

    Article Title: Detection of an immature dentate gyrus feature in human schizophrenia/bipolar patients

    doi: 10.1038/tp.2012.56

    Figure Lengend Snippet: Identification of immature dentate gyrus' (iDG) in a subset of schizophrenia/bipolar disorder (SCZ/BPD) patients. Expression of ( a ) calretinin, ( b ) calbindin and ( c ) PCNA ratios within the dentate gyrus (DG) of SCZ, BPD and major depressive disorder (MDD) cohorts. Calretinin was consistently overexpressed in DG neurons in SZ/BPD patients vscontrol ( d2–f2 ; boxed areas magnified in d3–f3 ; DAPI d1–f1 ). Elevated calretinin expression is positively associated with ( g ) suicide death, ( h ) psychosis diagnosis and ( i ) disease duration and ( j )negatively associated with age at disease onset. ( i–j ) SCZ = green, BPD = blue, MDD = brown. Scale bars: 1 mm ( d–f1, 2 ); 100 μm ( d–f3 ). P values: *

    Article Snippet: Primary antibodies included: BrdU (rat monoclonal, 1:500, Abcam; Cambridge, MA, USA), Calbindin (rabbit polyclonal, 1:1000; Swant, Bellinoza, Switzerland) and Calretinin (rabbit polyclonal, 1:1000; Swant).

    Techniques: Expressing

    Reduction of inhibitory interneurons and morphological alteration of the mitral cell layer in Tbr1 +/− olfactory bulbs. Double immunostaining of TBR2 and a VGLUT1, b VGLUT2, c Neurofilament-light chain (NF-LC), d Calretinin, e Parvalbumin, and f Calbindin was performed to compare olfactory bulbs of Tbr1 +/− and WT mice. g Quantification of Calretinin + interneurons at the glomerular layer (GL), external plexiform layer (EPL), mitral cell layer (MCL), and granular cell layer (GCL). h Quantification of parvalbumin + interneurons at the external plexiform layer (EPL). i Quantification of calbindin + interneurons at the glomerular layer (GL). Data represent mean plus SEM and the results of individual mice are shown. * p

    Journal: Molecular Autism

    Article Title: Haploinsufficiency of autism causative gene Tbr1 impairs olfactory discrimination and neuronal activation of the olfactory system in mice

    doi: 10.1186/s13229-019-0257-5

    Figure Lengend Snippet: Reduction of inhibitory interneurons and morphological alteration of the mitral cell layer in Tbr1 +/− olfactory bulbs. Double immunostaining of TBR2 and a VGLUT1, b VGLUT2, c Neurofilament-light chain (NF-LC), d Calretinin, e Parvalbumin, and f Calbindin was performed to compare olfactory bulbs of Tbr1 +/− and WT mice. g Quantification of Calretinin + interneurons at the glomerular layer (GL), external plexiform layer (EPL), mitral cell layer (MCL), and granular cell layer (GCL). h Quantification of parvalbumin + interneurons at the external plexiform layer (EPL). i Quantification of calbindin + interneurons at the glomerular layer (GL). Data represent mean plus SEM and the results of individual mice are shown. * p

    Article Snippet: The primary antibodies used in this report are as follows: rabbit anti-C-FOS (1:200; Cell Signaling), anti-calretinin (1:250; Swant), anti-calbidin (1:100; Cell Signaling), anti-neurofilament light chain (1:100; AB9568), anti-parvalbumin (1:200; Swant), rat anti-TBR2 (Eomes) (1:200; eBioscience 12-4875, PE-conjugated), anti-TBX21 (T-bet) (1:200; BD Biosciences), anti-VGLUT1 (1:200; Millipore AB5905), anti-VGLUT2 (1:200; Neuromab, 75-067).

    Techniques: Double Immunostaining, Mouse Assay

    EGFP-positive OFF bipolar cells ( A-D ) and ON bipolar cells ( F-H ). The injected cells are shown in red; the EGFP staining appears in green; and, as a reference for the stratification pattern, D and H show the calretinin immunolabeling in blue. A-D ). Injection of EGFP-positive cells with Alexa594-hydrazide resulted in the yellow appearance of the cell body, revealing that all OFF bipolar cell types express Cx45. F-H ). The yellow staining of injected somata shows that the three major types of cone ON bipolar cells (type 5-7) express Cx45. We have indicated the stratification levels in the IPL as S1-S5. The corresponding Nomarski images of the vertical retina sections are shown in E and I . The retinal layers are indicated: ONL (outer nuclear layer), OPL (outer plexiform layer), INL, and IPL. Scale bars, 20 μm.

    Journal: The Journal of Neuroscience

    Article Title: Deletion of Connexin45 in Mouse Retinal Neurons Disrupts the Rod/Cone Signaling Pathway between AII Amacrine and ON Cone Bipolar Cells and Leads to Impaired Visual Transmission

    doi: 10.1523/JNEUROSCI.3232-04.2005

    Figure Lengend Snippet: EGFP-positive OFF bipolar cells ( A-D ) and ON bipolar cells ( F-H ). The injected cells are shown in red; the EGFP staining appears in green; and, as a reference for the stratification pattern, D and H show the calretinin immunolabeling in blue. A-D ). Injection of EGFP-positive cells with Alexa594-hydrazide resulted in the yellow appearance of the cell body, revealing that all OFF bipolar cell types express Cx45. F-H ). The yellow staining of injected somata shows that the three major types of cone ON bipolar cells (type 5-7) express Cx45. We have indicated the stratification levels in the IPL as S1-S5. The corresponding Nomarski images of the vertical retina sections are shown in E and I . The retinal layers are indicated: ONL (outer nuclear layer), OPL (outer plexiform layer), INL, and IPL. Scale bars, 20 μm.

    Article Snippet: Then the slices were incubated in either Cx36 polyclonal antibodies (51-6300, diluted 1:500; Zymed, San Francisco, CA) or a calretinin antibody (diluted 1:250; Swant, Bellinzona, Switzerland) at 4°C overnight.

    Techniques: Injection, Staining, Immunolabeling

    Immunohistochemical staining of the tumor. (A) The cells were entirely positive for CK. (B) The tumor cells were strong positive for Calretinin. (C) Negative expression of CEA was present in the tumor cells. (D) Ki67 index was approximately 50% in mesothelima

    Journal: International Journal of Clinical and Experimental Pathology

    Article Title: Mesothelioma of the tunica vaginalis testis with prominent adenomatoid features: a case report

    doi:

    Figure Lengend Snippet: Immunohistochemical staining of the tumor. (A) The cells were entirely positive for CK. (B) The tumor cells were strong positive for Calretinin. (C) Negative expression of CEA was present in the tumor cells. (D) Ki67 index was approximately 50% in mesothelima

    Article Snippet: Tissue blocks were cut into 4-μm slides, deparaffinized in xylene, rehydrated with graded alcohols, and immunostained with the following antibodies: cytokeratin (CK, AE1/AE3, 1:50, DAKO), cytokeratin 5/6 (CK 5/6, 1:200, DAKO), cytokeratin 7 (CK 7, 1:200, DAKO), cytokeratin 20 (CK 20, 1:200, DAKO), vimentin (1:200, DAKO), calretinin (1:100, DAKO), CD30 (1:100, DAKO), carcino-embryonic antigen (CEA, 1:100, DAKO), α-fetoprofein (α-AFP, 1:200, DAKO), thyroid transcription factor 1 (TTF-1, 1:100, DAKO), Prostate Specific Antigen (PSA, 1:100, Santa cruz), S-100 (1:100, Santa Cruz), CD34 (1:100, DAKO), α-inhibin (1:100, DAKO), PLAP (1:100, DAKO), and Ki67 (1:200, DAKO).

    Techniques: Immunohistochemistry, Staining, Expressing

    Immunohistochemistry of AII amacrine cells, staining (green) for calretinin-positive ( arrows ) (A) , and quantitative measurement of cell numbers (B) . AII amacrine cells were lost after 7 days of OH and protected by 50 mg/kg SNJ-1945. Scale bar=20 μm.

    Journal: Journal of Ocular Pharmacology and Therapeutics

    Article Title: Degeneration and Dysfunction of Retinal Neurons in Acute Ocular Hypertensive Rats: Involvement of Calpains

    doi: 10.1089/jop.2013.0100

    Figure Lengend Snippet: Immunohistochemistry of AII amacrine cells, staining (green) for calretinin-positive ( arrows ) (A) , and quantitative measurement of cell numbers (B) . AII amacrine cells were lost after 7 days of OH and protected by 50 mg/kg SNJ-1945. Scale bar=20 μm.

    Article Snippet: The sections were then incubated overnight at 4°C with primary antibodies for protein kinase Cα (PKCα, 1:100; Cell Signaling Technologies, Beverly, MA) for visualization of rod bipolar cells and amacrine cells; Goα (1:50; Chemicon/Millipore, CA) for cone-ON bipolar and rod bipolar cells; calretinin (1:1,000; Santa Cruz Technologies, Santa Cruz, CA) for AII amacrine cells; calbindin (1:1,000; Swant, Bellinzona, Switzerland) for horizontal cells; and glial fibrillary acid protein (GFAP) (1:1,000.

    Techniques: Immunohistochemistry, Staining

    Histopathological sections showing tumor cells with glandular and papillary, cord-like structures (A) and a hobnail-like appearance of tumor cells with irregular and hyperchromatic nuclei resembling malignant mesothelioma (B). Immunohistochemical evaluation demonstrates the tumor cells to be positive for thyroid transcription factor-1 (C) and negative for calretinin (D).

    Journal: Journal of Pathology and Translational Medicine

    Article Title: Pseudomesotheliomatous carcinoma of the lung in the parietal pleura

    doi: 10.4132/jptm.2019.11.14

    Figure Lengend Snippet: Histopathological sections showing tumor cells with glandular and papillary, cord-like structures (A) and a hobnail-like appearance of tumor cells with irregular and hyperchromatic nuclei resembling malignant mesothelioma (B). Immunohistochemical evaluation demonstrates the tumor cells to be positive for thyroid transcription factor-1 (C) and negative for calretinin (D).

    Article Snippet: Calretinin (RTU, Ventana Medical Systems) , cytokeratin 5/6 (RTU, Ventana Medical Systems), Wilms’ tumor product-1 (1:100, Dako), and D2-40 (RTU, Ventana Medical Systems) were negative.

    Techniques: Immunohistochemistry

    ON cone bipolar cell subtypes were identified by immunohistochemistry after physiological recordings. The IPLs were marked using immunolabeling with calretinin or ChAT antibody. A , Subtype 5s ON bipolar cells (neurobiotin, green) identified with ChAT

    Journal: The Journal of Neuroscience

    Article Title: Roles of ON Cone Bipolar Cell Subtypes in Temporal Coding in the Mouse Retina

    doi: 10.1523/JNEUROSCI.3965-13.2014

    Figure Lengend Snippet: ON cone bipolar cell subtypes were identified by immunohistochemistry after physiological recordings. The IPLs were marked using immunolabeling with calretinin or ChAT antibody. A , Subtype 5s ON bipolar cells (neurobiotin, green) identified with ChAT

    Article Snippet: Then, the slice preparation was fixed with 4% paraformaldehyde for 30 min, incubated with streptavidin-conjugated Alexa Fluor 488 (1:200; Life Technologies) and anti-calretinin antibody (1:2000; EMD Millipore) overnight at 4°C, and then incubated with the secondary antibody for calretinin staining (anti-mouse Alexa Fluor 568, Life Technologies) for 2 h at room temperature.

    Techniques: Immunohistochemistry, Immunolabeling

    Morphologic characteristics of Prdm16+ ganglion cells. (A–D) A Thy1-YFP-H transgenic mouse retinal flatmount stained with antibodies to GFP (green); Prdm16 (red); and calretinin (gray). One round Prdm16+ nucleus coexpresses YFP (arrows) and its

    Journal: Investigative Ophthalmology & Visual Science

    Article Title: The Transcription Factor Prdm16 Marks a Single Retinal Ganglion Cell Subtype in the Mouse Retina

    doi: 10.1167/iovs.17-22442

    Figure Lengend Snippet: Morphologic characteristics of Prdm16+ ganglion cells. (A–D) A Thy1-YFP-H transgenic mouse retinal flatmount stained with antibodies to GFP (green); Prdm16 (red); and calretinin (gray). One round Prdm16+ nucleus coexpresses YFP (arrows) and its

    Article Snippet: The following primary reagents were used: mouse anti-Ap2α (1:250, 5E4-c; Developmental Hybridoma Studies Bank [DHSB], Iowa City, IA, USA); mouse anti-Brn3a (1:250, MAB-1585; Millipore Corp., Billerica, MA, USA); mouse anti-calretinin (1:500, MAB-1568; Millipore Corp); rabbit anti-cocaine and amphetamine regulated transcript (CART, 1:1000, H-003-62; Phoenix Pharmaceuticals, Burlingame, CA, USA); rabbit anti-Erg1 (1:250, ab92513; Abcam, Cambridge, MA, USA); chicken anti-GFP (1:1000, ab13970; Abcam); Griffonia simplicifolia isolectin B4-biotin ( Gsi B4, 1:250, B-1205; Vector Laboratories, Burlingame, CA, USA); mouse anti-Isl1/2 (1:100, 39.4D5-c, DHSB); rabbit anti-melanopsin (1:1000, gift from Russel Van Gelder, University of Washington) ; mouse anti-non-phosphorylated neurofilament H (Smi32, 1:500, Smi-32R; Covance, Princeton, NJ, USA); mouse anti-parvalbumin (1:250, PV25; Swant, Marly, Switzerland); rat anti-Pdgfrβ (1:250, NBP1-43349; Novus Biologicals, Littleton, CO, USA); goat anti-Prdm16 (1:20, sc55697; Santa Cruz Biotechnology, Dallas, TX, USA); sheep anti-Prdm16 (1 μg/mL, AF6295; R & D Systems, Minneapolis, MN, USA); rabbit anti-α-smooth muscle actin (αSMA, 1:100, ab5694; Abcam); and rabbit anti-Tbr2 (1:500, ab23345; Abcam).

    Techniques: Transgenic Assay, Staining

    A subset of mCHERRY-positive cell bodies in the midbrain, the LHb, the PMH, and the RMN are immuno-positive for CALBINDIN (CALB1) and CALRETININ (CALB2). Immunofluorescent histological analysis of ( A–G ) mCHERRY (red) and CALB1 (green) and ( H–N ) mCHERRY (red) and CALB2 (green) in the ( A , H ) midbrain, ( B , I ) arcuate nucleus, and ( C , J ) premammillary of the hypothalamus (PMH) ( D , K ), the lateral septum, ( E , L ) LHb, ( F , M ) BLP of the amygdala, and ( G , N ) RMN (yellow arrows illustrate example of cells where co-localization is detected; scale bar: 50 μm). Arc, arcuate nucleus; D3V, dorsal third ventricle; LS, lateral septum; MHb, medial habenula; PV, paraventricular thalamic nucleus; 3V, third ventricle; Arc, Arcuate nucleus; BLP, Basolateral amygdaloid nucleus posterior division; D3V, Dorsal third ventricle; LHb, Lateral habenula; LS, Lateral septum; MHb, Medial habenula; PMH, Premammillary nucleus of the hypothalamus; PV, Paraventricular thalamic nucleus; RMN, Retromammillary; SNc, Substantia nigra pars compacta; VTA: Ventral tegmental area; 3V, Third ventricle.

    Journal: eNeuro

    Article Title: Off-Target Effects in Transgenic Mice: Characterization of Dopamine Transporter (DAT)-Cre Transgenic Mouse Lines Exposes Multiple Non-Dopaminergic Neuronal Clusters Available for Selective Targeting within Limbic Neurocircuitry

    doi: 10.1523/ENEURO.0198-19.2019

    Figure Lengend Snippet: A subset of mCHERRY-positive cell bodies in the midbrain, the LHb, the PMH, and the RMN are immuno-positive for CALBINDIN (CALB1) and CALRETININ (CALB2). Immunofluorescent histological analysis of ( A–G ) mCHERRY (red) and CALB1 (green) and ( H–N ) mCHERRY (red) and CALB2 (green) in the ( A , H ) midbrain, ( B , I ) arcuate nucleus, and ( C , J ) premammillary of the hypothalamus (PMH) ( D , K ), the lateral septum, ( E , L ) LHb, ( F , M ) BLP of the amygdala, and ( G , N ) RMN (yellow arrows illustrate example of cells where co-localization is detected; scale bar: 50 μm). Arc, arcuate nucleus; D3V, dorsal third ventricle; LS, lateral septum; MHb, medial habenula; PV, paraventricular thalamic nucleus; 3V, third ventricle; Arc, Arcuate nucleus; BLP, Basolateral amygdaloid nucleus posterior division; D3V, Dorsal third ventricle; LHb, Lateral habenula; LS, Lateral septum; MHb, Medial habenula; PMH, Premammillary nucleus of the hypothalamus; PV, Paraventricular thalamic nucleus; RMN, Retromammillary; SNc, Substantia nigra pars compacta; VTA: Ventral tegmental area; 3V, Third ventricle.

    Article Snippet: Incubation of primary antibodies diluted in 0.1% PBS-T with 10% serum took place overnight at 4°C [rabbit TH 1:1000 #AB172, Millipore; chicken green fluorescent protein (GFP) 1:1000 #ab13970, Abcam (detects YFP); rabbit Calbindin (CALB1) 1:500 #AB1778, Millipore; rabbit Calretinin (CALB2) 1:500 #NBP1-88221, Novus Biologicals] or initiated for 2 h at RT and then overnight at 4°C (rat DAT 1:500 #MAB369, Millipore).

    Techniques: