Journal: BMC Cancer

Article Title: Uterine tumors with sex cord-like elements: a clinicopathologic study of 19 cases

doi: 10.1186/s12885-026-15591-5

Figure Lengend Snippet: Calretinin, α-inhibin, Melan A and steroidogenic factor-1 (SF-1) expression in uterine tumor resembling ovarian sex cord tumor (UTROSCT) and adenosarcoma (AS). A Diffuse calretinin expression in UTROSCT; ( B ) Dot-like α-inhibin expression in UTROSCT; ( C ) Polygonal cells in UTROSCT showed Melan A positive; ( D ) SF-1 expression in UTROSCT; ( E - H ) Calretinin, α-inhibin, Melan A and SF-1 expression in AS. (×200)

Article Snippet: Calretinin , Polyclonal , Zhongshan Golden Bridge Biotechnology LLC , Beijing , China , DAKO.

Techniques: Expressing

Journal: Stem Cell Research & Therapy

Article Title: SIRT1-modified human umbilical cord mesenchymal stem cells ameliorate experimental peritoneal fibrosis by inhibiting the TGF-β/Smad3 pathway

doi: 10.1186/s13287-020-01878-2

Figure Lengend Snippet: Oligonucleotide primer sequences for qRT-PCR

Article Snippet: The primary antibody of SIRT1 (ab110304, 1:1000 dilution), TGF-β (ab92486, 1:1000 dilution), α-SMA (ab265588, 1:1500 dilution), Fibronectin (ab268021, 1:2000 dilution), IL-6 (ab233706, 1:1000 dilution), IL-1β (ab234437, 1:1500 dilution), and Smad3 (ab40854, 1:2000 dilution) were purchased from Abcam (Cambridge, UK); pSmad3 (#8769, 1:1500 dilution), TNF-α (#3707, 1:1500 dilution), MCP-1 (#12199, 1:1000 dilution), Calretinin (#17114, 1:2000 dilution), Snail (#3879, 1:1000 dilution), and β-Actin (#4970, 1:3000 dilution) were obtained from Cell Signaling Technology, Inc. (Danvers, USA).

Techniques: Sequencing

Journal: Stem Cell Research & Therapy

Article Title: SIRT1-modified human umbilical cord mesenchymal stem cells ameliorate experimental peritoneal fibrosis by inhibiting the TGF-β/Smad3 pathway

doi: 10.1186/s13287-020-01878-2

Figure Lengend Snippet: SIRT1-modified hUCMSCs attenuated EMT in PD-induced peritoneal damage. a Immunohistochemical analyses of α-SMA expression in peritoneal tissues (bar 200 μm). b Accumulation of α-SMA-positive area of a . qRT-PCR was used to measure the mRNA levels of α-SMA ( c ), Fibronectin ( d ), Col III ( e ), Snail ( f ), and Calretinin ( g ) in the peritoneal omentum tissues from the indicated groups. h Western blot was used to measure the protein levels of Fibronectin, α-SMA, Snail, and Calretinin in the indicated conditions, and the relative expressions were normalized to control ( i ). Data are presented as mean ± SD. n = 8 for each group. * p < 0.05, ** p < 0.01, and *** p < 0.001 between the indicated groups

Article Snippet: The primary antibody of SIRT1 (ab110304, 1:1000 dilution), TGF-β (ab92486, 1:1000 dilution), α-SMA (ab265588, 1:1500 dilution), Fibronectin (ab268021, 1:2000 dilution), IL-6 (ab233706, 1:1000 dilution), IL-1β (ab234437, 1:1500 dilution), and Smad3 (ab40854, 1:2000 dilution) were purchased from Abcam (Cambridge, UK); pSmad3 (#8769, 1:1500 dilution), TNF-α (#3707, 1:1500 dilution), MCP-1 (#12199, 1:1000 dilution), Calretinin (#17114, 1:2000 dilution), Snail (#3879, 1:1000 dilution), and β-Actin (#4970, 1:3000 dilution) were obtained from Cell Signaling Technology, Inc. (Danvers, USA).

Techniques: Modification, Immunohistochemical staining, Expressing, Quantitative RT-PCR, Western Blot, Control

Journal: Cell reports

Article Title: RNA-programmable cell-type monitoring and manipulation in the human cortex with CellREADR

doi: 10.1016/j.celrep.2025.116037

Figure Lengend Snippet: (A) Human organotypic slice model. The human neocortex (middle and inferior temporal gyri; left) was sectioned at 350 μm and cultured on semi-permeable membranes for up to 16 days in vitro (DIV; right). CellREADR AAV vectors were applied to slices on DIV0. Orientation: A, anterior; D, dorsal; P, posterior; V, ventral. (B) Schematic of CellREADR design. In the single-virus design (left), binding of the sensor to the target initiates ADAR-mediated editing of a stop codon to express the downstream effector (mNeon). The binary system (right) uses a CellREADR virus (top right) to drive expression of tTA2 (Tet-advanced transactivator). A reporter virus, conditionally expressing a selected effector molecule (e.g., mNeon, ChIEF, or GCamp7f) under the control of the Tet-responsive element (TRE), is co-applied. (C) CellREADR targeting of diverse neuronal populations. Binary CellREADRs (mNeonGreen) designed against somatostatin ( SST ), Unc-5 netrin receptor B ( UNC5B ), parvalbumin ( PVALB ), forkhead box protein P2 ( FOXP2 ), and calretinin ( CALB2 ) were applied to the neocortex of at least 3 donors. Slices were immunostained for mNeon at DIV7. The CellREADR binary system drove robust expression of mNeon in differing lamina and in cells of various morphologies. Magnified images are indicated by dashed red boxes; throughout, pia and white matter (WM) are illustrated with dotted lines. Scale bars: 200 μm (large image) and 50 μm (magnified image). (D) Histological analysis of binary FOXP2 and CALB2 CellREADR targeting. CellREADR mNeon distribution (green, left) and protein expression of target (purple, middle) are depicted at DIV7. Fluorescence intensity profiles at far left reveal the depth distribution of cells targeted by CellREADR. Dashed boxes indicate areas depicted in (F). Scale bars: 200 μm. (E) Localization of CellREADR-targeted cells. CellREADR mNeon fluorescence intensity profiles measured across the depth of the cortex (0% = pial surface, 100% = WM boundary) averaged from 6 slices (tissues from 3 donors). The solid line shows the mean; the shaded area shows the SEM. FOXP2 -mNeon was relatively uniform throughout cortical layers, while CALB2 -mNeon was more restricted to outer layers. (F) Immunohistochemical characterization of CellREADR specificity. Representative images showing colocalization of CellREADR-mNeon with the corresponding target. Scale bars: 50 μm. (G) Quantification of CellREADR specificities as measured by immunostaining. Each point denotes the specificity of labeling measured from an individual donor’s tissue ( FOXP2 , n = 5 donors; binary CALB2 , n = 7; singular CALB2 , n = 3). Horizontal bars indicate mean specificity values for each CellREADR. Throughout the figures, data are presented as mean ± SEM. (H) Benchmarking CellREADR efficiency with a human interneuron enhancer virus DLX2.0-YFP. CALB2 CellREADR and DLX2.0-YFP viruses (rAAV2-retro) were applied to slices cut sequentially from the same neocortical tissue specimen. Tissue was immunostained at DIV7 against mNeon or YFP. Slice boundaries are indicated by the solid white line, and the WM is demarcated by a dashed white line; boxes mark the inset images on the bottom. Scale bars: 1 mm (top images) and 200 μm (bottom images). (I) Localization of DLX2.0-YFP expression. Representative image illustrating the efficiency and distribution of labeling achieved with a DLX2.0-YFP virus (left), with fluorescence intensity profile (right; averages from 3 donors) illustrating the distribution of labeled cells. Compared to CALB2 CellREADR labeling (D and H), DLX2.0-YFP + cells were less concentrated in the outer cortex. Scale bar: 250 μm. (J) DLX2.0-YFP targeting of the CALB2 population. Quantification of colocalization of DLX2.0-YFP expression and CALB2 measured by immunostaining (images not shown); note the inter-subject variability in the targeting of CALB2 cells ( n = 7 donors). (K) Efficiency of binary and singular CALB2 CellREADRs compared to DLX2.0-YFP enhancer virus. Quantification of cells labeled at DIV7 per unit area (binary CALB2 , n = 6 donors; singular CALB2 , n = 3; DLX2.0-YFP, n = 3). Note that although the CellREADRs were designed to target only CALB2 interneurons rather than multiple interneuron subclasses, they labeled more cells than the DLX2.0 virus (one-way ANOVA with Tukey’s post hoc).

Article Snippet: The following antibodies were used for immunohistochemical labeling of mNeon, mCherry, FoxP2, Calretinin and NeuN: mouse anti-mNeon Ab 1:500, Proteintech 32f6; chicken anti-mCherry Ab 1:500, Origene TA150127; rabbit anti-FoxP2 Ab 1:500, Abcam ab16046; rabbit anti-Calretinin Ab 1:500, Swant CR7697; mouse anti-NeuN Ab 1:500, Cell Signaling #94403S.

Techniques: Cell Culture, In Vitro, Virus, Binding Assay, Expressing, Control, Fluorescence, Immunohistochemical staining, Immunostaining, Labeling

Journal: BMC Medicine

Article Title: Safety and neuroprotective efficacy of the VCP inhibitor ML240 in large-animal and human retinal explants: a preclinical ex vivo study

doi: 10.1186/s12916-025-04610-0

Figure Lengend Snippet: VCP inhibition by ML240 does not affect other retinal cell types in macaque and human explants in vitro. Macaque and human retinal explants were cultured and treated with mPEG 5kDa -cholane-encapsulated ML240 (5 µM) or vehicle control for 8 DIV. A Inner retinal neurons were visualized by immunofluorescence staining with markers for bipolar cells (PCKα, first row), horizontal cells (calbindin, indicated by white arrows, second row), amacrine cells (calretinin, third row), and ganglion cells (RBPMS, fourth row). DAPI was used for nuclei counterstaining. Higher magnification images of the different cell types are presented in the upper right corner of every image. No differences were observed in terms of inner retinal structure and cell morphology between treated groups and vehicle control groups. Scale bar: 50 µm

Article Snippet: Primary antibodies were incubated overnight at 4 °C (Rabbit anti-iba1 (polyclonal), 1:500, Fujifilm Wako Chemicals, 019–19741; mouse anti-rhodopsin (monoclonal), 1:350, Sigma-Aldrich, MAB5316; rabbit anti-M opsin (polyclonal), 1:200, Sigma-Aldrich, AB5405; mouse anti-cone arrestin (monoclonal), 1:500, Millipore, MABN2636; mouse anti-PKCα (monoclonal), 1:300, Novus biologicals, NB600-201SS; rabbit anti-RNA-binding protein with multiple splicing (RBPMS) (polyclonal), 1:100, Novus biologicals, NBP2-20,112; mouse anti-calretinin (monoclonal), 1:300, Chemicon, MAB1568; chicken anti-calbindin (polyclonal), 1:500, Novus Biologicals, NBP2-50028SS).

Techniques: Inhibition, In Vitro, Cell Culture, Control, Immunofluorescence, Staining