Journal: Brazilian Journal of Medical and Biological Research

Article Title: Ropinirole alters gene expression profiles in SH-SY5Y cells: a whole genome microarray study

doi: 10.1590/1414-431X20154857

Figure Lengend Snippet: TaqMan¯ real-time PCR confirmation of microarray data in SH-SY5Y cells. CALM3 , EPS15 , RIPK5 , and PIK3C2B underwent TaqMan real-time PCR in SH-SY5Y cells. The y-axis represents the fold-change in expression after ROP treatment, and microarray and TaqMan data are plotted on the x-axis. There were no significant differences between the microarray and TaqMan data (P>0.05; Student's t- test).

Article Snippet: Assay IDs of genes CALM3 , EPS15 , RIPK5 , and PIK3C2B were Hs00270914_m1, Hs00179978_m1, Hs00418647_m1, and Hs00153248_m1, respectively.

Techniques: Real-time Polymerase Chain Reaction, Microarray, Expressing

Journal: Brazilian Journal of Medical and Biological Research

Article Title: Ropinirole alters gene expression profiles in SH-SY5Y cells: a whole genome microarray study

doi: 10.1590/1414-431X20154857

Figure Lengend Snippet: Validation of microarray data in HeLa cells. A , CALM3 , EPS15 , RIPK5 , and PIK3C2B underwent TaqMan ¯ real-time PCR in HeLa cells. The y-axis represents the fold-change in expression after ROP treatment, and microarray and TaqMan data are plotted on the x-axis. *P<0.05, TaqMan data compared to microarray data (Student's t -test). B , Western blot analysis in HeLa cells. β-actin was used as the loading control. PIC3C2B protein levels were elevated by treatment with 10 µM. *P<0.05, 10 µM ROP compared to control (Student's t -test).

Article Snippet: Assay IDs of genes CALM3 , EPS15 , RIPK5 , and PIK3C2B were Hs00270914_m1, Hs00179978_m1, Hs00418647_m1, and Hs00153248_m1, respectively.

Techniques: Biomarker Discovery, Microarray, Real-time Polymerase Chain Reaction, Expressing, Western Blot, Control

Journal: Cell reports

Article Title: Multiplexed drug-based selection and counterselection genetic manipulations in Drosophila

doi: 10.1016/j.celrep.2021.109700

Figure Lengend Snippet:

Article Snippet: pGAL4AD 768–881 , This work, available from AddGene , Cat# 165820.

Techniques: Virus, Recombinant, Purification, Gel Extraction, Plasmid Preparation, Software

Journal: EMBO Reports

Article Title: A retained intron in the 3′‐ UTR of Calm3 mRNA mediates its Staufen2‐ and activity‐dependent localization to neuronal dendrites

doi: 10.15252/embr.201744334

Figure Lengend Snippet: Primers and shRNA sequences

Article Snippet: Intermediate Calm3 3′‐UTR (Calm3 M ) was PCR‐amplified from a rat EST plasmid obtained from ImaGenes (IRBQp994H052D) (accession number AF231407 ), using the primers Calm3 M Fwd and Rev, and then cloned into psiCHECK2 plasmid via SalI/NotI.

Techniques: shRNA, Sequencing, Luciferase, Clone Assay, Northern Blot