calm1234 Search Results


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A) Representative MIP image of the apical surface area of a MCC expressing calmodulin-GFP. Calmodulin displays colocalization with the basal bodies and the apical actin network. B) Fluorescent intensity profile along the double headed arrow in A shows the colocalization of calmodulin with both basal bodies (centrin signal) and the apical actin network. C)Schematic depicting the localization of calmodulin in a MCC in relation to the basal bodies and the apical actin network. D) Representative images of the skin epithelium from control and calmodulin inhibitor treated embryos. MCC fail to fully integrate into the superficial skin epithelium when calmodulin activity is blocked, as evident by their small apical surface area. E) Quantification of the apical surface are of MCCs from control (n=100 MCCs) and calmodulin inhibitor-treated embryos (n=100 MCCs). two-sided, unpaired Student’s t -test. F) Representative images of the skin neuroepithelium from embryos expressing wild-type calmodulin or calcium binding deficient calmodulin mutant <t>(CALM1234).</t> Expression of CALM1234 results in defective MCC apical emergence (green arrows) while expression of WT calmodulin does not affect MCC epithelial integration (white arrows). G) Quantification of MCC epithelial integration upon expression of WT and calcium binding deficient mutant calmodulin. χ 2 test H) Quantification of the apical surface area of MCCs expressing WT and calcium binding deficient mutant calmodulin. N=80 control MCCs, 46 MCCs expressing WT calmodulin and 20 MCCs expressing mutant calmodulin. two-sided, unpaired Student’s t -test.
Calm1234, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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A) Representative MIP image of the apical surface area of a MCC expressing calmodulin-GFP. Calmodulin displays colocalization with the basal bodies and the apical actin network. B) Fluorescent intensity profile along the double headed arrow in A shows the colocalization of calmodulin with both basal bodies (centrin signal) and the apical actin network. C)Schematic depicting the localization of calmodulin in a MCC in relation to the basal bodies and the apical actin network. D) Representative images of the skin epithelium from control and calmodulin inhibitor treated embryos. MCC fail to fully integrate into the superficial skin epithelium when calmodulin activity is blocked, as evident by their small apical surface area. E) Quantification of the apical surface are of MCCs from control (n=100 MCCs) and calmodulin inhibitor-treated embryos (n=100 MCCs). two-sided, unpaired Student’s t -test. F) Representative images of the skin neuroepithelium from embryos expressing wild-type calmodulin or calcium binding deficient calmodulin mutant (CALM1234). Expression of CALM1234 results in defective MCC apical emergence (green arrows) while expression of WT calmodulin does not affect MCC epithelial integration (white arrows). G) Quantification of MCC epithelial integration upon expression of WT and calcium binding deficient mutant calmodulin. χ 2 test H) Quantification of the apical surface area of MCCs expressing WT and calcium binding deficient mutant calmodulin. N=80 control MCCs, 46 MCCs expressing WT calmodulin and 20 MCCs expressing mutant calmodulin. two-sided, unpaired Student’s t -test.

Journal: bioRxiv

Article Title: Calcium transients regulate epithelial integration of multiciliated cells during Xenopus skin development

doi: 10.1101/2024.11.01.621480

Figure Lengend Snippet: A) Representative MIP image of the apical surface area of a MCC expressing calmodulin-GFP. Calmodulin displays colocalization with the basal bodies and the apical actin network. B) Fluorescent intensity profile along the double headed arrow in A shows the colocalization of calmodulin with both basal bodies (centrin signal) and the apical actin network. C)Schematic depicting the localization of calmodulin in a MCC in relation to the basal bodies and the apical actin network. D) Representative images of the skin epithelium from control and calmodulin inhibitor treated embryos. MCC fail to fully integrate into the superficial skin epithelium when calmodulin activity is blocked, as evident by their small apical surface area. E) Quantification of the apical surface are of MCCs from control (n=100 MCCs) and calmodulin inhibitor-treated embryos (n=100 MCCs). two-sided, unpaired Student’s t -test. F) Representative images of the skin neuroepithelium from embryos expressing wild-type calmodulin or calcium binding deficient calmodulin mutant (CALM1234). Expression of CALM1234 results in defective MCC apical emergence (green arrows) while expression of WT calmodulin does not affect MCC epithelial integration (white arrows). G) Quantification of MCC epithelial integration upon expression of WT and calcium binding deficient mutant calmodulin. χ 2 test H) Quantification of the apical surface area of MCCs expressing WT and calcium binding deficient mutant calmodulin. N=80 control MCCs, 46 MCCs expressing WT calmodulin and 20 MCCs expressing mutant calmodulin. two-sided, unpaired Student’s t -test.

Article Snippet: For microinjection of DNA constructs, atub:UtrGFP, atub:GECO-RED, PACR (Addgene #55774 ) , atub:PACR, CALM-GFP (Addgene #47602), CALMWT (Addgene #111499), CALM1234 (Addgene #111518).

Techniques: Expressing, Control, Activity Assay, Binding Assay, Mutagenesis