Journal: Brain structure & function

Article Title: Global brain signal in awake rats

doi: 10.1007/s00429-019-01996-5

Figure Lengend Snippet: Correspondence between the global rsfMRI signal and neuronal spikes in the hippocampus. (A) Representative time courses of the global rsfMRI signal (blue) and calcium signal (green). Global signal peaks (blue arrows) and neural spikes (red arrows) in the hippocampus were strongly coupled within 2–6 sec of hemodynamic delay. (B) Permutation test of the correspondence between the global signal and GCaMP peaks (p = 0.016, 1000 permutations).

Article Snippet: Simultaneous calcium-rsfMRI signal recording A two-wavelength GCaMP fiber photometry system (Doric Lenses Inc., Quebec, Canada) was utilized for calcium signal recording ( ) ( ).

Techniques:

Journal: The Journal of Physiology

Article Title: Role of Orai1 and store-operated calcium entry in mouse lacrimal gland signalling and function

doi: 10.1113/jphysiol.2013.267740

Figure Lengend Snippet: Isolated lacrimal acinar cells were loaded with the fluorescent calcium indicator fura-5F. In the presence of 1.8 m m extracellular Ca 2+ , the responses to the phospholipase C-coupled agonist MeCh were compared in cells from WT and Orai1-KO mice.

Article Snippet: Lacrimal glands are responsive to both muscarinic and α-adrenergic stimulation, and the secretion of both fluid and protein appear to be dependent on the phospholipase C-coupled calcium signalling system (Dartt, ; Putney & Bird, ).

Techniques: Isolation, Mouse Assay

Journal: International Journal of Molecular Sciences

Article Title: IGF-1 Deficiency Rescue and Intracellular Calcium Blockade Improves Survival and Corresponding Mechanisms in a Mouse Model of Acute Kidney Injury

doi: 10.3390/ijms21114095

Figure Lengend Snippet: Favorable effects of IGF-1, BTP-2 monotherapies, and IGF-1 + BTP-2 combination therapies on LPS induced AKI associated major proinflammatory cytokines within the kidney. See text for interpretation. Data are mean ± SEM * p

Article Snippet: Treatment In a preventive, therapeutic strategy, some of the mice received a dose of ~3.0 × 106 TU in a 20 μL volume of an intramuscular injection of Lenti-IGF-1 ( ) 24 h before LPS injection; whereas, the calcium signaling blocker BTP-2 (16 mg/kg, Cayman Chemical, Ann Arbor, MI, USA) was administrated 1 h before the LPS injection.

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: IGF-1 Deficiency Rescue and Intracellular Calcium Blockade Improves Survival and Corresponding Mechanisms in a Mouse Model of Acute Kidney Injury

doi: 10.3390/ijms21114095

Figure Lengend Snippet: Favorable effects of IGF-1 and BTP-2 monotherapies and IGF-1 + BTP-2 combination therapy on LPS induced serum creatinine (Sr-CRT) and gene expression of the molecules reflecting kidney damage. See text for interpretation. Data are mean ± SEM * p

Article Snippet: Treatment In a preventive, therapeutic strategy, some of the mice received a dose of ~3.0 × 106 TU in a 20 μL volume of an intramuscular injection of Lenti-IGF-1 ( ) 24 h before LPS injection; whereas, the calcium signaling blocker BTP-2 (16 mg/kg, Cayman Chemical, Ann Arbor, MI, USA) was administrated 1 h before the LPS injection.

Techniques: Expressing

Journal: International Journal of Molecular Sciences

Article Title: IGF-1 Deficiency Rescue and Intracellular Calcium Blockade Improves Survival and Corresponding Mechanisms in a Mouse Model of Acute Kidney Injury

doi: 10.3390/ijms21114095

Figure Lengend Snippet: Favorable effects of therapies on LPS induced AKI expression of genes within the kidney related to the induction of calcium signaling. The target for BTP-2 is Orai1, which is decreased by this therapeutic agent as expected. See text for further interpretation. Data are mean ± SEM * p

Article Snippet: Treatment In a preventive, therapeutic strategy, some of the mice received a dose of ~3.0 × 106 TU in a 20 μL volume of an intramuscular injection of Lenti-IGF-1 ( ) 24 h before LPS injection; whereas, the calcium signaling blocker BTP-2 (16 mg/kg, Cayman Chemical, Ann Arbor, MI, USA) was administrated 1 h before the LPS injection.

Techniques: Expressing

Journal: International Journal of Molecular Sciences

Article Title: IGF-1 Deficiency Rescue and Intracellular Calcium Blockade Improves Survival and Corresponding Mechanisms in a Mouse Model of Acute Kidney Injury

doi: 10.3390/ijms21114095

Figure Lengend Snippet: Experimental design for acute kidney injury (AKI) parameters such as survival, serum IGF-1 rescue and gene expression analysis in lipopolysaccharide (LPS) induced AKI animals treated with IGF-1 and BTP-2.

Article Snippet: Treatment In a preventive, therapeutic strategy, some of the mice received a dose of ~3.0 × 106 TU in a 20 μL volume of an intramuscular injection of Lenti-IGF-1 ( ) 24 h before LPS injection; whereas, the calcium signaling blocker BTP-2 (16 mg/kg, Cayman Chemical, Ann Arbor, MI, USA) was administrated 1 h before the LPS injection.

Techniques: Expressing

Journal: International Journal of Molecular Sciences

Article Title: IGF-1 Deficiency Rescue and Intracellular Calcium Blockade Improves Survival and Corresponding Mechanisms in a Mouse Model of Acute Kidney Injury

doi: 10.3390/ijms21114095

Figure Lengend Snippet: Schematic molecular pathway responses to the induction of AKI by LPS and the preventative therapeutic counteractions by IGF-1 and BTP-2. The left panel shows the effects of LPS, while the right panel shows the effects of combination therapy, IGF-1 + BTP-2. LPS initiates the destructive process by activating TLR-4, which leads to an enhancement of IP3 receptor activity in the ER, which in turn enhances the calcium reflux from the ER to the cytosol. The depletion of ER calcium activates plasma membrane calcium influx, via Stim, through Orai1/TRPC6 channels. The consequent increase in cytosol calcium propels the pathway that leads to inflammation and injury, as described in the text. On the right panel, the red X indicates the sites of action of combination therapy (IGF-1 + BTP-2), which had positive effects on all of the adverse system changes caused by LPS administration. The action of BTP-2 is to specifically inhibit Orai1 and TRPC6, which counteracts the effect of LPS on calcium influx into the cytosol. The function of BTP-2 to inhibit the calcium channel regulator is complemented by IGF-1, which acts on SERCA to increase calcium influx into the ER from the cytosol. The consequent decrease in cytosolic calcium counteracts much of the adverse actions of LPS, as described in the text.

Article Snippet: Treatment In a preventive, therapeutic strategy, some of the mice received a dose of ~3.0 × 106 TU in a 20 μL volume of an intramuscular injection of Lenti-IGF-1 ( ) 24 h before LPS injection; whereas, the calcium signaling blocker BTP-2 (16 mg/kg, Cayman Chemical, Ann Arbor, MI, USA) was administrated 1 h before the LPS injection.

Techniques: Activity Assay, Reflux