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  • 99
    Thermo Fisher calcein acetoxymethyl ester
    Induction of EMT by eHSP90α in pancreatic ductal epithelial cells. (A) mRNA and protein levels of TCF12, Twist-1, Snail, Slug, E-cadherin, fibronectin, connexin-26, connexin-43, and GAPDH in HPDE cells treated with PBS, 15 μg/ml rHSP90α, or 15 μg/ml rHSP90α plus control IgG or anti-CD91 antibody. (B,C) The <t>Calcein-transfer</t> assay was performed to evaluate gap-junction activity of HPDE cells treated 24 h with PBS or 15 μg/ml of rHSP90α. Treated HPDE cells were labeled with Calcein <t>acetoxymethyl</t> ester and DiI dyes and then added to a monolayer of unstained, untreated HPDE cells for 0.2 or 3-h co-culture. Finally, the monolayer of cells were trypsinized and analyzed by flow cytometry. Representative dot plots are shown in (B). The cells in the R1 region were categorized as Calcein-accepting cells. The ratio of Calcein-accepting cells (designated as “% Transfer”) was quantified by the CellQuest software, and mean ± SD values of three independent experiments have been provided to indicate that cellular gap-junction activity was significantly inhibited after rHSP90α treatment (C). # , P
    Calcein Acetoxymethyl Ester, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/calcein acetoxymethyl ester/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    calcein acetoxymethyl ester - by Bioz Stars, 2021-07
    99/100 stars
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    99
    Millipore calcein
    Osteoblastic bone formation is reduced without sensory nerve innervation. a Representative images of immunofluorescence staining and quantitative analysis of the CGRP + sensory nerves (green) in the femurs of 12-week-old TrkA wt and TrkA Avil −/− mice. DAPI stains nuclei blue. Scale bar: 100 μm. b Representative micro-computed tomography (μCT) images of femurs from 12-week-old male TrkA wt and TrkA Avil −/− mice. Quantitative analysis of trabecular bone fraction (Tb. BV/TV) and trabecular number (Tb. N). Scale bar: 1 mm. c Histomorphological analysis of osteoblast (N.Ob/B.Pm) and osteoclast (N.Oc/B.Pm) numbers on the trabecular bone surface of femurs of 12-week-old TrkA wt and TrkA Avil −/− mice. d Trichrome staining and quantitative analysis of osteoid surface per bone surface (OS/BS) in femoral bone tissue from 12-week-old TrkA wt and TrkA Avil −/− mice. Scale bar, 50 μm. e ELISA analysis of serum OCN and CTX levels in 12-week-old TrkA wt and TrkA Avil −/− mice. f Representative images of <t>calcein</t> double labeling of trabecular bone of femurs with quantification of mineral apposition rate and bone formation rate in 12-week-old TrkA wt and TrkA Avil −/− mice. Scale bar, 20 μm. g Representative images of immunofluorescence staining and quantitative analysis of the CGRP + sensory nerves (green) in the vertebrae of 12-week-old TrkA wt and TrkA Avil −/− mice. DAPI stains nuclei blue. Scale bar: 100 μm. h Representative μCT images of vertebra from 12-week-old TrkA wt and TrkA Avil −/− mice. Quantitative analysis of trabecular bone fraction (Tb. BV/TV) and trabecular number (Tb. N). Scale bar: 1 mm. i Histomorphological analysis of osteoblast (N.Ob/B.Pm) numbers on the trabecular bone surface of 12-week-old TrkA wt and TrkA Avil −/− mice vertebra. N ≥ 5 per group. * P
    Calcein, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/calcein/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    calcein - by Bioz Stars, 2021-07
    99/100 stars
      Buy from Supplier

    99
    Millipore calcein am
    Analysis of the distribution of MSC within A) the “KG2/PVA‐cryogel” and B) the “KG2/PVA/polyP:NP‐cryogel” by cLSM. The cells were incubated for 8 h, then stained with <t>Calcein</t> AM and inspected. The compressed stacks are given in (A) and (B). In (C), individual slices from the poly‐free and the polyP‐containing cryogel (from the top to the bottom) are shown: C‐a–C‐c) aspects within the “KG2/PVA‐cryogel” and C‐d–C‐f) within “KG2/PVA/polyP:NP‐cryogel”. This series shows the increasing density of the cells within the polyP containing cryogel.
    Calcein Am, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/calcein am/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    calcein am - by Bioz Stars, 2021-07
    99/100 stars
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    N/A
    Calcein is a xanthene that is commonly used for the fluorometric determination of calcium in solution As this form of calcein is not membrane permeable it can be used in
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    N/A
    Calcein Blue is a metallofluorochromic indicator for EDTA titration of calcium at high pH and copper nickel and cobalt at low pH Calcein Blue demonstrates enhanced fluorescence upon binding Ca
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    N/A
    Calcein UltraBlue is a membrane impermeant fluorescent dye It is a rich blue emitting variant of the green fluorophore calcein Item No 16221 which has been entrapped in liposomes and
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    N/A
    Calcein Blue is a membrane impermeant fluorescent dye It is a blue emitting variant of the green fluorophore Calcein Item No 16221 which has been entrapped in liposomes and similar
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    Image Search Results


    Induction of EMT by eHSP90α in pancreatic ductal epithelial cells. (A) mRNA and protein levels of TCF12, Twist-1, Snail, Slug, E-cadherin, fibronectin, connexin-26, connexin-43, and GAPDH in HPDE cells treated with PBS, 15 μg/ml rHSP90α, or 15 μg/ml rHSP90α plus control IgG or anti-CD91 antibody. (B,C) The Calcein-transfer assay was performed to evaluate gap-junction activity of HPDE cells treated 24 h with PBS or 15 μg/ml of rHSP90α. Treated HPDE cells were labeled with Calcein acetoxymethyl ester and DiI dyes and then added to a monolayer of unstained, untreated HPDE cells for 0.2 or 3-h co-culture. Finally, the monolayer of cells were trypsinized and analyzed by flow cytometry. Representative dot plots are shown in (B). The cells in the R1 region were categorized as Calcein-accepting cells. The ratio of Calcein-accepting cells (designated as “% Transfer”) was quantified by the CellQuest software, and mean ± SD values of three independent experiments have been provided to indicate that cellular gap-junction activity was significantly inhibited after rHSP90α treatment (C). # , P

    Journal: Oncoimmunology

    Article Title: Myeloid-derived macrophages and secreted HSP90α induce pancreatic ductal adenocarcinoma development

    doi: 10.1080/2162402X.2018.1424612

    Figure Lengend Snippet: Induction of EMT by eHSP90α in pancreatic ductal epithelial cells. (A) mRNA and protein levels of TCF12, Twist-1, Snail, Slug, E-cadherin, fibronectin, connexin-26, connexin-43, and GAPDH in HPDE cells treated with PBS, 15 μg/ml rHSP90α, or 15 μg/ml rHSP90α plus control IgG or anti-CD91 antibody. (B,C) The Calcein-transfer assay was performed to evaluate gap-junction activity of HPDE cells treated 24 h with PBS or 15 μg/ml of rHSP90α. Treated HPDE cells were labeled with Calcein acetoxymethyl ester and DiI dyes and then added to a monolayer of unstained, untreated HPDE cells for 0.2 or 3-h co-culture. Finally, the monolayer of cells were trypsinized and analyzed by flow cytometry. Representative dot plots are shown in (B). The cells in the R1 region were categorized as Calcein-accepting cells. The ratio of Calcein-accepting cells (designated as “% Transfer”) was quantified by the CellQuest software, and mean ± SD values of three independent experiments have been provided to indicate that cellular gap-junction activity was significantly inhibited after rHSP90α treatment (C). # , P

    Article Snippet: HPDE cells treated with PBS or 15 μg/ml of rHSP90α were trypsinized and stained with Calcein acetoxymethyl ester and DiI (Invitrogen) for use as dye-donor cells.

    Techniques: Activity Assay, Labeling, Co-Culture Assay, Flow Cytometry, Cytometry, Software

    Osteoblastic bone formation is reduced without sensory nerve innervation. a Representative images of immunofluorescence staining and quantitative analysis of the CGRP + sensory nerves (green) in the femurs of 12-week-old TrkA wt and TrkA Avil −/− mice. DAPI stains nuclei blue. Scale bar: 100 μm. b Representative micro-computed tomography (μCT) images of femurs from 12-week-old male TrkA wt and TrkA Avil −/− mice. Quantitative analysis of trabecular bone fraction (Tb. BV/TV) and trabecular number (Tb. N). Scale bar: 1 mm. c Histomorphological analysis of osteoblast (N.Ob/B.Pm) and osteoclast (N.Oc/B.Pm) numbers on the trabecular bone surface of femurs of 12-week-old TrkA wt and TrkA Avil −/− mice. d Trichrome staining and quantitative analysis of osteoid surface per bone surface (OS/BS) in femoral bone tissue from 12-week-old TrkA wt and TrkA Avil −/− mice. Scale bar, 50 μm. e ELISA analysis of serum OCN and CTX levels in 12-week-old TrkA wt and TrkA Avil −/− mice. f Representative images of calcein double labeling of trabecular bone of femurs with quantification of mineral apposition rate and bone formation rate in 12-week-old TrkA wt and TrkA Avil −/− mice. Scale bar, 20 μm. g Representative images of immunofluorescence staining and quantitative analysis of the CGRP + sensory nerves (green) in the vertebrae of 12-week-old TrkA wt and TrkA Avil −/− mice. DAPI stains nuclei blue. Scale bar: 100 μm. h Representative μCT images of vertebra from 12-week-old TrkA wt and TrkA Avil −/− mice. Quantitative analysis of trabecular bone fraction (Tb. BV/TV) and trabecular number (Tb. N). Scale bar: 1 mm. i Histomorphological analysis of osteoblast (N.Ob/B.Pm) numbers on the trabecular bone surface of 12-week-old TrkA wt and TrkA Avil −/− mice vertebra. N ≥ 5 per group. * P

    Journal: Nature Communications

    Article Title: Prostaglandin E2 mediates sensory nerve regulation of bone homeostasis

    doi: 10.1038/s41467-018-08097-7

    Figure Lengend Snippet: Osteoblastic bone formation is reduced without sensory nerve innervation. a Representative images of immunofluorescence staining and quantitative analysis of the CGRP + sensory nerves (green) in the femurs of 12-week-old TrkA wt and TrkA Avil −/− mice. DAPI stains nuclei blue. Scale bar: 100 μm. b Representative micro-computed tomography (μCT) images of femurs from 12-week-old male TrkA wt and TrkA Avil −/− mice. Quantitative analysis of trabecular bone fraction (Tb. BV/TV) and trabecular number (Tb. N). Scale bar: 1 mm. c Histomorphological analysis of osteoblast (N.Ob/B.Pm) and osteoclast (N.Oc/B.Pm) numbers on the trabecular bone surface of femurs of 12-week-old TrkA wt and TrkA Avil −/− mice. d Trichrome staining and quantitative analysis of osteoid surface per bone surface (OS/BS) in femoral bone tissue from 12-week-old TrkA wt and TrkA Avil −/− mice. Scale bar, 50 μm. e ELISA analysis of serum OCN and CTX levels in 12-week-old TrkA wt and TrkA Avil −/− mice. f Representative images of calcein double labeling of trabecular bone of femurs with quantification of mineral apposition rate and bone formation rate in 12-week-old TrkA wt and TrkA Avil −/− mice. Scale bar, 20 μm. g Representative images of immunofluorescence staining and quantitative analysis of the CGRP + sensory nerves (green) in the vertebrae of 12-week-old TrkA wt and TrkA Avil −/− mice. DAPI stains nuclei blue. Scale bar: 100 μm. h Representative μCT images of vertebra from 12-week-old TrkA wt and TrkA Avil −/− mice. Quantitative analysis of trabecular bone fraction (Tb. BV/TV) and trabecular number (Tb. N). Scale bar: 1 mm. i Histomorphological analysis of osteoblast (N.Ob/B.Pm) numbers on the trabecular bone surface of 12-week-old TrkA wt and TrkA Avil −/− mice vertebra. N ≥ 5 per group. * P

    Article Snippet: Briefly, we injected 0.1% calcein (Sigma-Aldrich, C0875) in phosphate-buffered saline at a concentration of 10mg per kg into the mice subcutaneously 7 days and 1 day before sacrifice.

    Techniques: Immunofluorescence, Staining, Mouse Assay, Micro-CT, Enzyme-linked Immunosorbent Assay, Labeling

    Osteoblastic bone formation is blunted after sensory denervation. a Representative images of immunofluorescence staining and quantitative analysis of the CGRP + sensory nerves in the femurs of 8-week-old iDTR Avil +/− mice injected with vehicle or 1 ug per kg per day DTX 3 time a week for four consecutive weeks. Scale bar: 100 μm. b Representative μCT images of femurs from iDTR Avil +/− mice injected with vehicle or DTX. Quantitative analysis of trabecular bone fraction and trabecular number. Scale bar: 1 mm. c Histomorphological analysis of the osteoblast (N.Ob/B.Pm) and osteoclast (N.Oc/B.Pm) numbers on the trabecular bone surface of femurs of iDTR Avil +/− mice injected with vehicle or DTX. d Representative trichrome staining and quantitative analysis of OS/BS in femoral bone tissue from iDTR Avil +/− mice injected with vehicle or DTX. Scale bar, 50 μm. e ELISA analysis of serum OCN and CTX levels in iDTR Avil +/− mice injected with vehicle or DTX. f Representative images of calcein double labeling of femoral trabecular bone with quantification of MAR and BFR in iDTR Avil +/− mice injected with vehicle or DTX. Scale bar, 20 μm. g Representative images of immunofluorescence staining and quantitative analysis of the CGRP + sensory nerves (green) in the vertebra of iDTR Avil +/− mice injected with vehicle or DTX. DAPI stains nuclei blue. Scale bar: 100 μm. h Representative μCT images of vertebrae from iDTR Avil +/− mice injected with vehicle or DTX. Quantitative analysis of trabecular bone fraction (Tb. BV/TV) and trabecular number (Tb. N). Scale bar: 1 mm. i Histomorphological analysis of osteoblast (N.Ob/B.Pm) numbers on the trabecular bone surface of 12-week-old TrkA wt and TrkA Avil −/− mice vertebra. N ≥ 5 per group. * P

    Journal: Nature Communications

    Article Title: Prostaglandin E2 mediates sensory nerve regulation of bone homeostasis

    doi: 10.1038/s41467-018-08097-7

    Figure Lengend Snippet: Osteoblastic bone formation is blunted after sensory denervation. a Representative images of immunofluorescence staining and quantitative analysis of the CGRP + sensory nerves in the femurs of 8-week-old iDTR Avil +/− mice injected with vehicle or 1 ug per kg per day DTX 3 time a week for four consecutive weeks. Scale bar: 100 μm. b Representative μCT images of femurs from iDTR Avil +/− mice injected with vehicle or DTX. Quantitative analysis of trabecular bone fraction and trabecular number. Scale bar: 1 mm. c Histomorphological analysis of the osteoblast (N.Ob/B.Pm) and osteoclast (N.Oc/B.Pm) numbers on the trabecular bone surface of femurs of iDTR Avil +/− mice injected with vehicle or DTX. d Representative trichrome staining and quantitative analysis of OS/BS in femoral bone tissue from iDTR Avil +/− mice injected with vehicle or DTX. Scale bar, 50 μm. e ELISA analysis of serum OCN and CTX levels in iDTR Avil +/− mice injected with vehicle or DTX. f Representative images of calcein double labeling of femoral trabecular bone with quantification of MAR and BFR in iDTR Avil +/− mice injected with vehicle or DTX. Scale bar, 20 μm. g Representative images of immunofluorescence staining and quantitative analysis of the CGRP + sensory nerves (green) in the vertebra of iDTR Avil +/− mice injected with vehicle or DTX. DAPI stains nuclei blue. Scale bar: 100 μm. h Representative μCT images of vertebrae from iDTR Avil +/− mice injected with vehicle or DTX. Quantitative analysis of trabecular bone fraction (Tb. BV/TV) and trabecular number (Tb. N). Scale bar: 1 mm. i Histomorphological analysis of osteoblast (N.Ob/B.Pm) numbers on the trabecular bone surface of 12-week-old TrkA wt and TrkA Avil −/− mice vertebra. N ≥ 5 per group. * P

    Article Snippet: Briefly, we injected 0.1% calcein (Sigma-Aldrich, C0875) in phosphate-buffered saline at a concentration of 10mg per kg into the mice subcutaneously 7 days and 1 day before sacrifice.

    Techniques: Immunofluorescence, Staining, Mouse Assay, Injection, Enzyme-linked Immunosorbent Assay, Labeling

    Deletion of PGE2 receptor EP4 in sensory nerve results in bone loss. a Double-immunofluorescence images of femoral bone sections from 12-week-old EP4 wt or EP4 Avil −/− mice using antibodies against EP4 (red) and CGRP (green). DAPI stains nuclei blue. Scale bar, 50 μm. b , c Representative μCT images of femurs from 12-week-old EP4 wt and EP4 Avil −/− mice. Quantitative analysis of trabecular bone fraction (Tb. BV/TV), trabecular number (Tb. N), cortical thickness (Ct. Th), and cortical bone volume (Cor. BV). d ELISA analysis of serum PGE2 level in 12-week-old EP4 wt and EP4 Avil −/− mice. e Representative images of immunostaining and quantitative analysis of the COX2 + cells (in brown) on trabecular bone surface of femoral bone from 12-week-old EP4 wt and EP4 Avil −/− mice. Scale bar, 20 μm. f Histomorphological analysis of osteoblast (N.Ob/B.Pm) and osteoclast (N.Oc/B.Pm) numbers on the trabecular bone surface of femurs of 12-week-old EP4 wt and EP4 Avil −/− mice. g ELISA analysis of serum OCN and CTX levels in 12-week-old EP4 wt and EP4 Avil −/− mice. h Representative trichrome staining and quantitative analysis of osteoid surface per bone surface (OS/BS) in femoral bone tissue of 12-week-old EP4 wt and EP4 Avil −/− mice. Scale bar, 50 μm. i , j Ten-week-old EP4 wt and EP4 Avil −/− mice were injected with vehicle or 3 mg per kg per day PGE2 for 3 consecutive days, and bone samples were harvested 12 days after injection. Calcein was injected 5 days and 1 day before sacrifice. Representative images of calcein double labeling of femoral trabecular bone with quantification of mineral apposition rate (MAR) and bone formation rate (BFR). Scale bar, 20 μm. N ≥ 5 per group. * P

    Journal: Nature Communications

    Article Title: Prostaglandin E2 mediates sensory nerve regulation of bone homeostasis

    doi: 10.1038/s41467-018-08097-7

    Figure Lengend Snippet: Deletion of PGE2 receptor EP4 in sensory nerve results in bone loss. a Double-immunofluorescence images of femoral bone sections from 12-week-old EP4 wt or EP4 Avil −/− mice using antibodies against EP4 (red) and CGRP (green). DAPI stains nuclei blue. Scale bar, 50 μm. b , c Representative μCT images of femurs from 12-week-old EP4 wt and EP4 Avil −/− mice. Quantitative analysis of trabecular bone fraction (Tb. BV/TV), trabecular number (Tb. N), cortical thickness (Ct. Th), and cortical bone volume (Cor. BV). d ELISA analysis of serum PGE2 level in 12-week-old EP4 wt and EP4 Avil −/− mice. e Representative images of immunostaining and quantitative analysis of the COX2 + cells (in brown) on trabecular bone surface of femoral bone from 12-week-old EP4 wt and EP4 Avil −/− mice. Scale bar, 20 μm. f Histomorphological analysis of osteoblast (N.Ob/B.Pm) and osteoclast (N.Oc/B.Pm) numbers on the trabecular bone surface of femurs of 12-week-old EP4 wt and EP4 Avil −/− mice. g ELISA analysis of serum OCN and CTX levels in 12-week-old EP4 wt and EP4 Avil −/− mice. h Representative trichrome staining and quantitative analysis of osteoid surface per bone surface (OS/BS) in femoral bone tissue of 12-week-old EP4 wt and EP4 Avil −/− mice. Scale bar, 50 μm. i , j Ten-week-old EP4 wt and EP4 Avil −/− mice were injected with vehicle or 3 mg per kg per day PGE2 for 3 consecutive days, and bone samples were harvested 12 days after injection. Calcein was injected 5 days and 1 day before sacrifice. Representative images of calcein double labeling of femoral trabecular bone with quantification of mineral apposition rate (MAR) and bone formation rate (BFR). Scale bar, 20 μm. N ≥ 5 per group. * P

    Article Snippet: Briefly, we injected 0.1% calcein (Sigma-Aldrich, C0875) in phosphate-buffered saline at a concentration of 10mg per kg into the mice subcutaneously 7 days and 1 day before sacrifice.

    Techniques: Immunofluorescence, Mouse Assay, Enzyme-linked Immunosorbent Assay, Immunostaining, Staining, Injection, Labeling

    Analysis of the distribution of MSC within A) the “KG2/PVA‐cryogel” and B) the “KG2/PVA/polyP:NP‐cryogel” by cLSM. The cells were incubated for 8 h, then stained with Calcein AM and inspected. The compressed stacks are given in (A) and (B). In (C), individual slices from the poly‐free and the polyP‐containing cryogel (from the top to the bottom) are shown: C‐a–C‐c) aspects within the “KG2/PVA‐cryogel” and C‐d–C‐f) within “KG2/PVA/polyP:NP‐cryogel”. This series shows the increasing density of the cells within the polyP containing cryogel.

    Journal: Advanced Science

    Article Title: In Situ Polyphosphate Nanoparticle Formation in Hybrid Poly(vinyl alcohol)/Karaya Gum Hydrogels: A Porous Scaffold Inducing Infiltration of Mesenchymal Stem Cells

    doi: 10.1002/advs.201801452

    Figure Lengend Snippet: Analysis of the distribution of MSC within A) the “KG2/PVA‐cryogel” and B) the “KG2/PVA/polyP:NP‐cryogel” by cLSM. The cells were incubated for 8 h, then stained with Calcein AM and inspected. The compressed stacks are given in (A) and (B). In (C), individual slices from the poly‐free and the polyP‐containing cryogel (from the top to the bottom) are shown: C‐a–C‐c) aspects within the “KG2/PVA‐cryogel” and C‐d–C‐f) within “KG2/PVA/polyP:NP‐cryogel”. This series shows the increasing density of the cells within the polyP containing cryogel.

    Article Snippet: The samples were coated with gold; in a second one the cryogels were exposed to Calcein AM (#17783; Sigma) to analyze the cells within the cryogel by cLSM.

    Techniques: Confocal Laser Scanning Microscopy, Incubation, Staining