Journal: eLife

Article Title: LKB1 coordinates neurite remodeling to drive synapse layer emergence in the outer retina

doi: 10.7554/eLife.56931

Figure Lengend Snippet: Antibodies used in LKB1 mutant tissue analysis. Antibodies were utilized that label individual neuron populations and synapses in the outer retina.

Article Snippet: Calbindin D-28k , Full-length recombinant human Calbindin D-28K , Horizontal cells; amacrine cells; retinal ganglion cells , Novus biologicals; chicken polyclonal; NBP2-50028; no RRID , 1:2000.

Techniques: Mutagenesis, Labeling, Concentration Assay, Recombinant, Derivative Assay, Purification

Journal: PLoS Genetics

Article Title: Rgs6 is Required for Adult Maintenance of Dopaminergic Neurons in the Ventral Substantia Nigra

doi: 10.1371/journal.pgen.1004863

Figure Lengend Snippet: (A) Rgs6 expression revealed by immunohistofluorescence (red) together with TH (green) in mDA neurons of SNc but not VTA. Scale bar 410 µm. (B) Co-immunofluorescence analysis of TH (green) and Rgs6 or Pitx3 (red, as indicated) in SNc and VTA of adult mice. Arrowheads point to Pitx3-negative or Rgs6-negative mDA neurons in dorsal SNc. Scale bar 100 µm. (C) Triple immunofluorescence staining for TH (green), Pitx3 (red) and Calb1 (blue) on tissue sections of adult mice indicates that the majority of TH+Pitx3− cells of dSNc (arrowheads) are positive for Calb1, while TH+Pitx3+ cells of vSNc are negative for Calb1, consistent with depiction in A. Scale bar 100 µm.

Article Snippet: Primary antibodies used were against Pitx3 (rabbit home-made, 1∶400), Rgs6 (rabbit home-made 1∶25), Th (Millipore MAB318, 1∶1000), Th (Millipore AB152, 1∶500), Calb1 (R&D Systems AF3320, 1∶40), Fgf10 (Millipore ABN44, 1∶1000), Slc6a3 (Santa Cruz sc-32258, 1∶250), Drd2 (Santa Cruz sc5303, 1∶100), Aldh1a1 (Abcam ab24343, 1∶400), BDNF (Abcam ab108319, 1∶25), phospho-p27 (Abcam ab32096, 1∶50), phospho-Erk1/2 (Cell Signaling 4376, 1∶25), DJ-1 (Abcam ab18257, 1∶500) Lrrk2 (Epitomics 3514-1, 1∶50), Pink1 (Novus Biologicals BC100-494, 1∶50), LC3B (Cell Signaling 3868, 1∶50), and Vmat2 (Millipore AB1598P, 1∶200).

Techniques: Expressing, Immunohistofluorescence, Immunofluorescence, Staining

Journal: Frontiers in Neural Circuits

Article Title: Spatial distribution of D1R- and D2R-expressing medium-sized spiny neurons differs along the rostro-caudal axis of the mouse dorsal striatum

doi: 10.3389/fncir.2013.00124

Figure Lengend Snippet: Striatal interneurons distribution in the D2R/A2aR-expressing MSNs-poor zone of the caudal striatum . Striatal sections at the caudal level from Drd2-EGFP mice ( n = 5 for each staining) labeled with antibodies for choline acetyltransferase (ChAT, A ), parvalbumin (ParV, B ), neuropeptide Y (NPY, C ) and calretinin (CalR, D ). Scale bars, 200 μm. Insets, higher magnification (GFP, green, A 1 ; ChAT, magenta, A 2 ; merge, A 3 ), (GFP, green, B 1 ; ParV, magenta, B 2 ; merge, B 3 ), (GFP, green, C 1 ; NPY, magenta, C 2 ; merge C 3 ) and (GFP, green, D 1 ; CalR, magenta, D 2 ; merge D 3 ). Scale bars, 100 μm. DStr, dorsal striatum; GPe, external globus pallidus, GFP, green fluorescent protein.

Article Snippet: Finally, they were incubated overnight or 72 h at 4°C with the primary antibodies: chicken and rabbit anti-GFP (1:500 and 1:1000 respectively, Invitrogen), rabbit anti-vesicular glutamate transporter 1 (VGluT1) or anti-VGluT2 (1:1000 gift from S. El Mestikawy), mouse anti-tyrosine hydroxylase (TH) (1:1000, Millipore), rat anti-dopamine transporter (DAT) (1:1000, Millipore), mouse anti-NeuN (1:500, Millipore), mouse anti-D1R (1:500 gift from R. R. Luedtke), rabbit anti-Gα olf (1:500) (Hervé et al., ), rabbit anti-β-galactosidase (1:1000, Cappel, MP Biomedicals), guinea-pig anti-MOR (1:500 gift from T. Kaneko) mouse anti-DARPP-32 (1:1000 gift from P. Greengard), rabbit anti-calretinin (CalR), anti-calbindin-D28k and anti-parvalbumin (ParV) (1:1000, Swant), rabbit anti-neuropeptide Y (NPY) (1:400, Abcam), goat anti-ChAT (1:400, Millipore), rabbit anti-substance P (1:500, Millipore), rabbit anti-preproenkephalin (ppENK) (1:500, Neuromics) and rabbit anti-RFP (1:1000, MBL).

Techniques: Expressing, Staining, Labeling

Journal: PLOS Pathogens

Article Title: Deciphering infected cell types, hub gene networks and cell-cell communication in infectious bronchitis virus via single-cell RNA sequencing

doi: 10.1371/journal.ppat.1012232

Figure Lengend Snippet: (a) White arrows indicate the localization of IBV N protein in AQP2-expressing collecting duct cells. Green fluorescence shows positive staining for IBV N and red fluorescence shows staining for AQP2. (b) White arrows indicate the localization of IBV N protein in CALB1-expressing distal tubule cells. Red fluorescence shows positive staining for IBV N and green fluorescence shows staining for CALB1. (c) Changes in cell communication numbers: The top network diagram shows cell clusters as nodes, with line thickness indicating changes in communication numbers. The lower heatmap details these changes, with rows representing signal-sending cells and columns indicating signal-receiving cells. The color scale reflects the inter-group differences in signal communication frequency between different cell types (number of communications in the infected group—number in control group). The bar plots at the top and right side represent the overall differences in the number of signals sent/received by specific cell clusters. (d) Changes in cell communication strength: Similar to (c), with the top network diagram displaying changes in communication strength (communication strength in the infected group—strength in the control group). In the lower heatmap, the color scale reflects the inter-group differences in signal communication strength between different cell types. The bar plots at the top and right side represent the overall differences in the strength of signals sent/received by specific cell clusters (infected group—control group). (e) Inter-group differences in the communication strength of specific signaling pathways (receptor-ligand pairs) across cell clusters. Rows represent signal pathways and columns correspond to cell clusters, with heatmap colors depicting the strength variation of signals (infect group vs. control group). Upper left triangles for signal sent and lower right triangles for signal received. The bar plot on the right side shows the overall difference in communication strength of these signaling pathways between the IBV-infected group and the control group.

Article Snippet: mouse mab calb1 , 1:200 , Boster Bio (BM0203).

Techniques: Expressing, Fluorescence, Staining, Infection, Control, Protein-Protein interactions

Journal: PLOS Pathogens

Article Title: Deciphering infected cell types, hub gene networks and cell-cell communication in infectious bronchitis virus via single-cell RNA sequencing

doi: 10.1371/journal.ppat.1012232

Figure Lengend Snippet: Antbodies and reagents used in present study.

Article Snippet: mouse mab calb1 , 1:200 , Boster Bio (BM0203).

Techniques: Blocking Assay, Immunofluorescence

Journal: The Journal of Neuroscience

Article Title: Sex-Dependent Regulation of Aromatase-Mediated Synaptic Plasticity in the Basolateral Amygdala

doi: 10.1523/JNEUROSCI.1532-16.2016

Figure Lengend Snippet: AROM expression in major subregions of rodent amygdala. A, Coronal section through anterior amygdala of an young adult mouse, immunostained for AROM using rabbit polyclonal antibodies (Yague et al., 2006). Substantial AROM expression is detectable in the MeA and CeA, in the BL and La nucleus of the BLA, and in the adjacent piriform cortex (Pir). Virtually, no AROM immunoreactivity is seen in the BM of BLA. Low levels of expression were found in the cortical amygdala (CoA). Scale bar, 250 μm. B, Western blots showing AROM protein expression in amygdala subregions CeA, BLA, and MeA of juvenile male and female rats using monoclonal antibodies against AROM (Acris). Tissue from hippocampus (Hip), cerebellum (Cer), and somatosensory cortex (Cor) was blotted for comparison. The strongest signal is found in the MeA. AROM expression levels in the CeA and BLA are comparable to levels in the hippocampus, neocortex, and cerebellum (note: a representative band for the female CeA was inserted from a different gel). C, Quantitative analysis of Western blot data, comparing AROM expression in BLA of age-matched (P20–P24) juvenile male and female rats. No difference between the sexes was evident (rel. expression of AROM: 0.46 ± 0.06 in females; 0.48 ± 0.09 in males; p = 0.88; n = 8 of each sex).

Article Snippet: Rabbit polyclonal anti-calbindin D28K antibodies (Calb; 1:2000; Merck Millipore, Billerica, MA, USA, Cat# AB1778, RRID:AB_2068336) were applied for characterization of the cultures.

Techniques: Expressing, Western Blot, Bioprocessing, Comparison