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Image Search Results
Journal: Frontiers in Endocrinology
Article Title: Biphasic expression of thyroid hormone receptor TRβ1 in mammalian retina and anterior ocular tissues
doi: 10.3389/fendo.2023.1174600
Figure Lengend Snippet: TRβ1 localization in cones and inner retinal neurons detected in Thrb b1 lacZ reporter mice. (A) , TRβ1 (β-galactosidase, bgal protein immunofluorescence) co-staining with cone markers (TRβ2, Arr3) in Thrb +/ b1 mice. (B) , TRβ1 (bgal) in amacrine (arrowheads) and horizontal cells (arrows) shown by staining for calbindin ( left ). Staining was sometimes weak and appeared punctate. TRβ1 was also detected in ganglion cells (arrowheads) by staining for RBPMS ( right ). In panel (B) , Thrb b1/b1 homozygotes were analyzed to enhance detection of bgal. For RBPMS, a general nuclear stain (DAPI, blue) shows tissue background. AC, amacrine cell; dAC, displaced AC; GC, ganglion cell; HC, horizontal cell; INL, inner nuclear layer; ipl, inner plexiform layer; IS/OS, inner/outer segments; ONL, outer nuclear layer; opl, outer plexiform layer.
Article Snippet: Primary antibodies ( target antigen, type, dilution, source, RRID ): TRβ2, rabbit polyclonal, 1:2,000 ( ) (RRID : AB_2927439); β-galactosidase, chicken polyclonal, 1:500 (Abcam ab9361, RRID : AB_307210); Arr3, rabbit polyclonal, 1:1,000 (Millipore, AB15282, RRID : AB_1163387);
Techniques: Immunofluorescence, Staining